Cytokeratin 8 Monoclonal Antibody (8A5D12) | Invitrogen MA5-15460 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cytokeratin 8 Monoclonal Antibody (8A5D12)

catalog :

MA5-15460

quantity :

100 µL

price :

US 400.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8A5D12

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	Wrzesinski K, Rogowska Wrzesinska A, Kanlaya R, Borkowski K, SchwÃ¤mmle V, Dai J, et al. The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments. PLoS ONE. 2014;9:e106973 pubmed publisher

image

image 1 :

Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 antibody.

product information

Product Type :

Antibody

Product Name :

Cytokeratin 8 Monoclonal Antibody (8A5D12)

Catalog # :

MA5-15460

Quantity :

100 µL

Price :

US 400.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human

Clone :

8A5D12

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Immunogen :

Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

AA960620; AL022697; AU019895; C CY K2C8; CARD2; cb186; CK 8; CK8; CK-8; CYK8; CYKER; cytokeratin 8; cytokeratin 8 (370 AA); cytokeratin endo A; Cytokeratin-8; cytokeratin-A; DreK8; EndoA; etID33550.23; etID43142.23; etID43142.8; hard keratin; K2C8; K8; K-8; KERA; keratin 8; keratin 8, type II; keratin complex 2, basic, gene 8; keratin, type 2, gene 8; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8-like protein; keratin-8; KO; Krt-2.8; Krt2-8; krt8; LOW QUALITY PROTEIN: keratin, type II cytoskeletal 8; sb:cb186; type II cytokeratin A (no.8); type-II keratin Kb8; wu:fa20h05; wu:fa95h10; wu:fb96c06; zfk8; zf-K8

more info or order :

Invitrogen product webpage