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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GFP Monoclonal Antibody (GF28R)
catalog :
MA5-15256
quantity :
100 µg
price :
US 237.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GF28R
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 92
Reference
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image
image 1 :
Invitrogen MA5-15256 image 1
1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1ug (2) and 10ug (3) of cell lysates used
product information
Product Type :
Antibody
Product Name :
GFP Monoclonal Antibody (GF28R)
Catalog # :
MA5-15256
Quantity :
100 µg
Price :
US 237.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Tag
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 1:500-1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:3,000
Species :
Tag
Clone :
GF28R
Isotype :
IgG1
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Immunogen :
GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 1:500-1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000-1:3,000
Aliases :
GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA