Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (E.665.10)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (E.665.10)

catalog :

MA5-15228

quantity :

200 µL

price :

US 449.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

E.665.10

reactivity :

Chinese hamsters, human, mouse, rat, zebrafish

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5d	Beauvais D, Jung O, Yang Y, Sanderson R, Rapraeger A. Syndecan-1 (CD138) Suppresses Apoptosis in Multiple Myeloma by Activating IGF1 Receptor: Prevention by SynstatinIGF1R Inhibits Tumor Growth. Cancer Res. 2016;76:4981-93 pubmed publisher
immunohistochemistry - paraffin section; zebrafish ; 1:100	de Oliveira S, Boudinot P, Calado Ã, Mulero V. Duox1-derived H2O2 modulates Cxcl8 expression and neutrophil recruitment via JNK/c-JUN/AP-1 signaling and chromatin modifications. J Immunol. 2015;194:1523-33 pubmed publisher
	Yang H, Song B, Sim B, Jung Y, Chae U, Lee D, et al. Establishment and Characterization of Immortalized Miniature Pig Pancreatic Cell Lines Expressing Oncogenic K-RasG12D. Int J Mol Sci. 2020;21: pubmed publisher

image

image 1 :

Flow cytometric analysis of Phospho-JNK on Jurkat cells left untreated (green histogram) or treated with anisomycin (blue histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-15228) at a dilution of 1:200 for 1 hour at room temperature. As a negative control anisomycin-treated cells were stained with an isotype control (red histogram). Following staining with either MA5-15228 or isotype control, cells were stained with a fluorescently-conjugated anti-mouse IgG secondary antibody and analyzed on a flow cytometer.

image 2 :

Immunofluorescent analysis of Phospho-JNK (green) in HeLa cells either left untreated (left panel) or treated with 25ug/ml anisomycin (right panel) for 25 min. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-15228) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

product information

Product Type :

Antibody

Product Name :

Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (E.665.10)

Catalog # :

MA5-15228

Quantity :

200 µL

Price :

US 449.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Hamster, Human, Mouse, Rat, Yeast

Applications :

ChIP assay: 1-3 µL, Flow Cytometry: 1:400, Immunocytochemistry: 1:200, Immunoprecipitation: 1:250, Western Blot: 1:2,000

Species :

Hamster, Human, Mouse, Rat, Yeast

Clone :

E.665.10

Isotype :

IgG1

Storage :

-20°C

Description :

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding pThr183/Tyr185 of human SAPK/JNK

Format :

Liquid

Applications w/Dilutions :

ChIP assay: 1-3 µL, Flow Cytometry: 1:400, Immunocytochemistry: 1:200, Immunoprecipitation: 1:250, Western Blot: 1:2,000

Aliases :

AI849689; AI851083; C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; I79_012561; JNK; JNK 55; JNK/SAPK alpha; JNK1; JNK1 beta1 protein kinase; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK-46; JNK-55; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; Mapk8; Mapk9; mitogen activated protein kinase 8; mitogen activated protein kinase 9; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 9; OTTHUMP00000019552; p54 gamma; p54a; p54-alpha; p54aSAPK; Phospho-JNK; PRKM; PRKM8; Prkm9; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK; SAPK 1; SAPK gamma; SAPK1; SAPK1A; SAPK1c; SAPK-alpha; stress activated protein kinase alpha II; stress-activated protein kinase 1; stress-activated protein kinase 1a; Stress-activated protein kinase 1c; stress-activated protein kinase JNK1; Stress-activated protein kinase JNK2

more info or order :

Invitrogen product webpage