Phospho-NFkB p65 (Ser536) Monoclonal Antibody (T.849.2) | Invitrogen MA5-15160 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-NFkB p65 (Ser536) Monoclonal Antibody (T.849.2)

catalog :

MA5-15160

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

T.849.2

reactivity :

Chinese hamsters, human, mouse, rat, dogs, pigs , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 3e	Gong F, Gu J, Wang H. Up regulated Tmbim1 activation promotes high fat diet (HFD)-induced cardiomyopathy by enhancement of inflammation and oxidative stress. Biochem Biophys Res Commun. 2018;504:797-804 pubmed publisher
western blot; human; fig 3f	Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
western blot; pigs ; 1:1000; fig 2	Ganesan S, Reynolds C, Hollinger K, Pearce S, Gabler N, Baumgard L, et al. Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2016;310:R1288-96 pubmed publisher
western blot; human; 1:1000; fig 4d	Jia D, Tan Y, Liu H, Ooi S, Li L, Wright K, et al. Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo. Oncotarget. 2016;7:771-85 pubmed publisher
western blot; human	Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, et al. Î²-Catenin and NF-ÎºB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Cell Death Differ. 2015;22:298-310 pubmed publisher
immunohistochemistry; domestic rabbit; 1:1000	Fouad A, Jresat I. Therapeutic potential of cannabidiol against ischemia/reperfusion liver injury in rats. Eur J Pharmacol. 2011;670:216-23 pubmed publisher
immunohistochemistry; rat; 1:100	Fouad A, Al Sultan A, Yacoubi M. Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats. Eur J Pharmacol. 2011;655:91-8 pubmed publisher
	Ji L, Wang T, Tian L, Song H, Gao M. Roxatidine inhibits fibrosis by inhibiting NF‑κB and MAPK signaling in macrophages sensing breast implant surface materials. Mol Med Rep. 2020;21:161-172 pubmed publisher
	Jia D, Li L, Andrew S, Allan D, Li X, Lee J, et al. An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells. Cell Death Dis. 2017;8:e2932 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-NFkB p65 (Ser536) Monoclonal Antibody (T.849.2)

Catalog # :

MA5-15160

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Canine, Hamster, Human, Mouse, Non-human primate, Porcine, Rat

Applications :

Flow Cytometry: 1:1,600, Immunocytochemistry: 1:1,600, Immunoprecipitation: 1:50, Western Blot: 1:1,000

Species :

Canine, Hamster, Human, Mouse, Non-human primate, Porcine, Rat

Clone :

T.849.2

Isotype :

IgG

Storage :

-20°C

Description :

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. Phosphorylation of p65 at Ser 536 located in the carboxy-terminal transactivation domains (TAD) leads to nuclear localization of the transcriptionally active complex and NF-kB mediated transactivation of several downstream genes. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding pSer536 of human NFkB p65

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:1,600, Immunocytochemistry: 1:1,600, Immunoprecipitation: 1:50, Western Blot: 1:1,000

Aliases :

avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; I79_021812; MGC131774; NF KB; NFkappaB p65; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kappaB transcription factor p65 subunit; NFkB; NF-kB p65 subunit; nfkb rela; NFKB1; nfkb3; NFkB-p50; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; P65 transcription factor; putative transcription factor p65 homolog; REL A; rel1; rela; RELA proto-oncogene, NF-kB subunit; rela.L; rela-a; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A L homeolog; v-rel reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65; XELAEV_18022301mg; Xrel1; xrela

more info or order :

Invitrogen product webpage