Phospho-SMAD2 (Ser465, Ser467) Monoclonal Antibody (H.205.4) | Invitrogen MA5-15122 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-SMAD2 (Ser465, Ser467) Monoclonal Antibody (H.205.4)

catalog :

MA5-15122

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

H.205.4

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; fig 4	Liu W, Zhou L, Zhou C, Zhang S, Jing J, Xie L, et al. GDF11 decreases bone mass by stimulating osteoclastogenesis and inhibiting osteoblast differentiation. Nat Commun. 2016;7:12794 pubmed publisher
western blot; human; loading ...; fig 2a	Egerman M, Cadena S, Gilbert J, Meyer A, Nelson H, Swalley S, et al. GDF11 Increases with Age and Inhibits Skeletal Muscle Regeneration. Cell Metab. 2015;22:164-74 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-SMAD2 (Ser465, Ser467) Monoclonal Antibody (H.205.4)

Catalog # :

MA5-15122

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mink, Mouse, Rat

Applications :

Immunocytochemistry: 1:100, Western Blot: 1:1,000

Species :

Human, Mink, Mouse, Rat

Clone :

H.205.4

Isotype :

IgG

Storage :

-20°C

Description :

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding pSer465/467 of human Smad2

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100, Western Blot: 1:1,000

Aliases :

7120426M23Rik; EGK_09664; hMAD-2; hSMAD2; JV18; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Madh2; Madr2; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against decapentaplegic-like protein 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2; Smad-2; Smad2Deltaexon3

more info or order :

Invitrogen product webpage