Phospho-IkB alpha (Ser32) Monoclonal Antibody (B.142.7) | Invitrogen MA5-15087 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-IkB alpha (Ser32) Monoclonal Antibody (B.142.7)

catalog :

MA5-15087

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

B.142.7

reactivity :

guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs

application :

western blot, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5b	Rasheed S, Leong H, Lakshmanan M, Raju A, Dadlani D, Chong F, et al. GNA13 expression promotes drug resistance and tumor-initiating phenotypes in squamous cell cancers. Oncogene. 2018;37:1340-1353 pubmed publisher
	Jiang J, Qi L, Lv Z, Jin S, Wei X, Shi F. Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens. Antioxidants (Basel). 2019;8: pubmed publisher
	Lee J, Ryu H, Lee S, Kim M, Kwon O, Kim M, et al. Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharide‑induced pulmonary inflammation by inhibiting interleukin‑8 production and NF‑κB activation. Int J Mol Med. 2019;44:949-959 pubmed publisher
	Wang L, Yan Z, Vihinen H, Eriksson O, Wang W, Soliymani R, et al. FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function. J Cell Biol. 2019;218:97-111 pubmed publisher

image

image 1 :

product information

Product Type :

Antibody

Product Name :

Phospho-IkB alpha (Ser32) Monoclonal Antibody (B.142.7)

Catalog # :

MA5-15087

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Bovine, Canine, Chicken, Guinea Pig, Human, Mouse, Non-human primate, Porcine, Rat

Applications :

Immunoprecipitation: 1:100, Western Blot: 1:1,000

Species :

Bovine, Canine, Chicken, Guinea Pig, Human, Mouse, Non-human primate, Porcine, Rat

Clone :

B.142.7

Isotype :

IgG

Storage :

-20°C

Description :

IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding pSer32 of human IkB alpha.

Format :

Liquid

Applications w/Dilutions :

Immunoprecipitation: 1:100, Western Blot: 1:1,000

Aliases :

AI462015; ECI-6; ECI-6/IKBA; I kappa B-alpha; I(Kappa)B(alpha); I79_018146; IkappaB alpha; IkappaBalpha; I-kappaBalpha; I-kappa-B-alpha; Ikba; IKBalpha; IKB-alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappaB inhibitor alpha; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; Nfkbia; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; Rel-associated pp40; REL-associated protein pp40; RL/IF-1

more info or order :

Invitrogen product webpage