Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4) | Invitrogen MA5-15033 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4)

catalog :

MA5-15033

quantity :

100 µL

price :

US 474

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

G.305.4

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3f	Sun H, Jiang M, Fu X, Cai Q, Zhang J, Yin Y, et al. Mesencephalic astrocyte-derived neurotrophic factor reduces cell apoptosis via upregulating HSP70 in SHSY-5Y cells. Transl Neurodegener. 2017;6:12 pubmed publisher
western blot; human; fig 4	Chusri P, Kumthip K, Hong J, Zhu C, Duan X, Jilg N, et al. HCV induces transforming growth factor Î²1 through activation of endoplasmic reticulum stress and the unfolded protein response. Sci Rep. 2016;6:22487 pubmed publisher
western blot; mouse; 1:1000; fig 6e	Treacy Abarca S, Mukherjee S. Legionella suppresses the host unfolded protein response via multiple mechanisms. Nat Commun. 2015;6:7887 pubmed publisher
western blot; mouse; 1:1000; fig 6e	Duś Szachniewicz K, Wozniak M, Nelke K, Gamian E, Gerber H, Ziółkowski P. Protein tyrosine phosphatase receptor R and Z1 expression as independent prognostic indicators in oral squamous cell carcinoma. Head Neck. 2015;37:1816-22 pubmed publisher
	Lu J, Xiong K, Qian X, Choi J, Shim Y, Burnett J, et al. Spata7 is required for maintenance of the retinal connecting cilium. Sci Rep. 2022;12:5575 pubmed publisher
	Weingärtner M, St xfc cheli S, Jebbawi F, Gottstein B, Beldi G, Lundstr xf6 m Stadelmann B, et al. Albendazole reduces hepatic inflammation and endoplasmic reticulum-stress in a mouse model of chronic Echinococcus multilocularis infection. PLoS Negl Trop Dis. 2022;16:e0009192 pubmed publisher
	Dion W, Ballance H, Lee J, Pan Y, Irfan S, Edwards C, et al. Four-dimensional nuclear speckle phase separation dynamics regulate proteostasis. Sci Adv. 2022;8:eabl4150 pubmed publisher
	Baral A, Park P. Leptin Induces Apoptotic and Pyroptotic Cell Death via NLRP3 Inflammasome Activation in Rat Hepatocytes. Int J Mol Sci. 2021;22: pubmed publisher
	Pu H, Qian Q, Wang F, Gong M, Ge X. Schizandrin A induces the apoptosis and suppresses the proliferation, invasion and migration of gastric cancer cells by activating endoplasmic reticulum stress. Mol Med Rep. 2021;24: pubmed publisher
	Du R, Sullivan D, Azizian N, Liu Y, Li Y. Inhibition of ERAD synergizes with FTS to eradicate pancreatic cancer cells. BMC Cancer. 2021;21:237 pubmed publisher
	Arioka Y, Shishido E, Kushima I, Suzuki T, Saito R, Aiba A, et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons. EBioMedicine. 2021;63:103138 pubmed publisher
	Moghadami A, Aboutalebi Vand Beilankouhi E, Kalantary Charvadeh A, Hamzavi M, Mosayyebi B, Sedghi H, et al. Inhibition of USP14 induces ER stress-mediated autophagy without apoptosis in lung cancer cell line A549. Cell Stress Chaperones. 2020;25:909-917 pubmed publisher
	Tang Y, Chen H, Yi Y, Chen G, Yang F, Li Y, et al. Inhibition of eIF2α Dephosphorylation Protects Hepatocytes from Apoptosis by Alleviating ER Stress in Acute Liver Injury. Biomed Res Int. 2020;2020:2626090 pubmed publisher
	Huang H, Tian X, Peng X, Huang L, Mei L, Zhan Y, et al. Antifungal itraconazole ameliorates experimental autoimmune encephalomyelitis through a novel mechanism of action. Adv Clin Exp Med. 2020;29:535-545 pubmed publisher
	Zhang Z, Cui D, Zhang T, Sun Y, Ding S. Swimming Differentially Affects T2DM-Induced Skeletal Muscle ER Stress and Mitochondrial Dysfunction Related to MAM. Diabetes Metab Syndr Obes. 2020;13:1417-1428 pubmed publisher
	Zeng J, Jin Q, Ruan Y, Sun C, Xu G, Chu M, et al. Inhibition of TGFβ-activated protein kinase 1 ameliorates myocardial ischaemia/reperfusion injury via endoplasmic reticulum stress suppression. J Cell Mol Med. 2020;24:6846-6859 pubmed publisher
	Kim H, Joe Y, Rah S, Kim S, Park S, Park J, et al. Carbon monoxide-induced TFEB nuclear translocation enhances mitophagy/mitochondrial biogenesis in hepatocytes and ameliorates inflammatory liver injury. Cell Death Dis. 2018;9:1060 pubmed publisher

image

image 1 :

Western blot analysis of pancreatic acinar (AR42J) cells using Phospho-PERK pThr980 Monoclonal Antibody (Product # MA5-15033). Membranes containing whole cell extracts from untreated (-) AR42J cells or AR42J cells treated (+) for 20 minutes with thapsigargin (Tg) were probed with MA5-15033 followed by an anti-rabbit IgG secondary antibody.

product information

Product Type :

Antibody

Product Name :

Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4)

Catalog # :

MA5-15033

Quantity :

100 µL

Pricing :

US 474

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Mouse, Rat

Applications :

Western Blot: 1:1,000

Species :

Mouse, Rat

Clone :

G.305.4

Isotype :

IgG

Storage :

-20°C

Description :

PERK, a member of the GCN2 subfamily of Ser/Thr protein kinases, phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It likely serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1 Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction Expression of this Type I membrane protein is ubiquitous, with highest levels seen in secretory tissues. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding pThr980 of mouse PERK

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:1,000

Aliases :

2.7.11.1; AI427929; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PEK; Perk; PRKR-like endoplasmic reticulum kinase; WRS

more info or order :

Invitrogen product webpage