p21 Monoclonal Antibody (R.229.6) | Invitrogen MA5-14949 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p21 Monoclonal Antibody (R.229.6)

catalog :

MA5-14949

quantity :

100 µL

price :

US 496.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

R.229.6

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:800; loading ...; fig 2d	Bloniarz D, Adamczyk Grochala J, Lewinska A, Wnuk M. The lack of functional DNMT2/TRDMT1 gene modulates cancer cell responses during drug-induced senescence. Aging (Albany NY). 2021;13:15833-15874 pubmed publisher
immunocytochemistry; human; 1:300; loading ...; fig 3	Bellotti C, Capanni C, Lattanzi G, Donati D, Lucarelli E, Duchi S. Detection of mesenchymal stem cells senescence by prelamin A accumulation at the nuclear level. Springerplus. 2016;5:1427 pubmed publisher
immunohistochemistry; human; 1:50	Gerashchenko B, Salmina K, Eglitis J, Huna A, Grjunberga V, Erenpreisa J. Disentangling the aneuploidy and senescence paradoxes: a study of triploid breast cancers non-responsive to neoadjuvant therapy. Histochem Cell Biol. 2016;145:497-508 pubmed publisher
western blot; human; fig 3a	Huna A, Salmina K, Erenpreisa J, Vazquez Martin A, Krigerts J, Inashkina I, et al. Role of stress-activated OCT4A in the cell fate decisions of embryonal carcinoma cells treated with etoposide. Cell Cycle. 2015;14:2969-84 pubmed publisher
	Moreno Valladares M, Moncho Amor V, Silva T, Garc xe9 s J, xc1 lvarez Satta M, Matheu A. KRT5+/p63+ Stem Cells Undergo Senescence in the Human Lung with Pathological Aging. Aging Dis. 2023;14:1013-1027 pubmed publisher
	Filla M, Faralli J, Dunn C, Khan H, Peters D. NFATc1 Regulation of Dexamethasone-Induced TGFB2 Expression Is Cell Cycle Dependent in Trabecular Meshwork Cells. Cells. 2023;12: pubmed publisher
	Jung Y, Chae C, Choi G, Shin H, Lim J, Chang H, et al. Cyanidin 3-O-arabinoside suppresses DHT-induced dermal papilla cell senescence by modulating p38-dependent ER-mitochondria contacts. J Biomed Sci. 2022;29:17 pubmed publisher
	Lewi x144 ska A, Przybylski P, Adamczyk Grochala J, B x142 oniarz D, Litwinienko G, Wnuk M. Senolysis-Based Elimination of Chemotherapy-Induced Senescent Breast Cancer Cells by Quercetin Derivative with Blocked Hydroxy Groups. Cancers (Basel). 2022;14: pubmed publisher
	Pennycook B, Barr A. Palbociclib-mediated cell cycle arrest can occur in the absence of the CDK inhibitors p21 and p27. Open Biol. 2021;11:210125 pubmed publisher
	Arnold R, Vehns E, Randl H, Djabali K. Baricitinib, a JAK-STAT Inhibitor, Reduces the Cellular Toxicity of the Farnesyltransferase Inhibitor Lonafarnib in Progeria Cells. Int J Mol Sci. 2021;22: pubmed publisher
	Schwartz R, Shokhirev M, Andrade L, Gutkind J, Iglesias Bartolomé R, Shadel G. Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes. Aging (Albany NY). 2021;13:4747-4777 pubmed publisher
	Charruyer A, Weisenberger T, Li H, Khalifa A, Schroeder A, Belzer A, et al. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis. Aging Cell. 2021;20:e13310 pubmed publisher
	Squarzoni S, Schena E, Sabatelli P, Mattioli E, Capanni C, Cenni V, et al. Interleukin-6 neutralization ameliorates symptoms in prematurely aged mice. Aging Cell. 2021;20:e13285 pubmed publisher
	Krenacs T, Meggyeshazi N, Forika G, Kiss E, Hamar P, Székely T, et al. Modulated Electro-Hyperthermia-Induced Tumor Damage Mechanisms Revealed in Cancer Models. Int J Mol Sci. 2020;21: pubmed publisher
	Pierozan P, Cattani D, Karlsson O. Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) induce epigenetic alterations and promote human breast cell carcinogenesis in vitro. Arch Toxicol. 2020;94:3893-3906 pubmed publisher
	Kim S, Chae C, Lee H, Jung Y, Choi G, Kim J, et al. Sodium butyrate inhibits high cholesterol-induced neuronal amyloidogenesis by modulating NRF2 stabilization-mediated ROS levels: involvement of NOX2 and SOD1. Cell Death Dis. 2020;11:469 pubmed publisher
	Cenni V, Squarzoni S, Loi M, Mattioli E, Lattanzi G, Capanni C. Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin-BAF Interaction and BAF Nuclear Localization. Cells. 2020;9: pubmed publisher
	Liu C, Arnold R, Henriques G, Djabali K. Inhibition of JAK-STAT Signaling with Baricitinib Reduces Inflammation and Improves Cellular Homeostasis in Progeria Cells. Cells. 2019;8: pubmed publisher
	Lewinska A, Adamczyk Grochala J, Bloniarz D, Olszowka J, Kulpa Greszta M, Litwinienko G, et al. AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized Fe3O4 nanoparticles during oxidant-induced senescence in human fibroblasts. Redox Biol. 2020;28:101337 pubmed publisher
	Ahmad F, Leake D. Lysosomal oxidation of LDL alters lysosomal pH, induces senescence, and increases secretion of pro-inflammatory cytokines in human macrophages. J Lipid Res. 2019;60:98-110 pubmed publisher
	Lewinska A, Bocian A, Petrilla V, Adamczyk Grochala J, Szymura K, Hendzel W, et al. Snake venoms promote stress-induced senescence in human fibroblasts. J Cell Physiol. 2019;234:6147-6160 pubmed publisher
	Mattioli E, Andrenacci D, Garofalo C, Prencipe S, Scotlandi K, Remondini D, et al. Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS. Aging Cell. 2018;17:e12824 pubmed publisher
	Pierozan P, Jernerén F, Karlsson O. Perfluorooctanoic acid (PFOA) exposure promotes proliferation, migration and invasion potential in human breast epithelial cells. Arch Toxicol. 2018;92:1729-1739 pubmed publisher
	Pierozan P, Karlsson O. PFOS induces proliferation, cell-cycle progression, and malignant phenotype in human breast epithelial cells. Arch Toxicol. 2018;92:705-716 pubmed publisher
	Lewinska A, Adamczyk Grochala J, Kwasniewicz E, Deregowska A, Semik E, Zabek T, et al. Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression. Redox Biol. 2018;14:20-34 pubmed publisher
	Verreault M, Schmitt C, Goldwirt L, Pelton K, Haidar S, Levasseur C, et al. Preclinical Efficacy of the MDM2 Inhibitor RG7112 in MDM2-Amplified and TP53 Wild-type Glioblastomas. Clin Cancer Res. 2016;22:1185-96 pubmed publisher
	Waby J, Chirakkal H, Yu C, Griffiths G, Benson R, Bingle C, et al. Sp1 acetylation is associated with loss of DNA binding at promoters associated with cell cycle arrest and cell death in a colon cell line. Mol Cancer. 2010;9:275 pubmed publisher

product information

Product Type :

Antibody

Product Name :

p21 Monoclonal Antibody (R.229.6)

Catalog # :

MA5-14949

Quantity :

100 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Human, Non-human primate

Applications :

Flow Cytometry: 1:100-1:400, Immunocytochemistry: 1:800, Immunohistochemistry (Paraffin): 1:50, Immunoprecipitation: 1:50, Western Blot: 1:1,000

Species :

Human, Non-human primate

Clone :

R.229.6

Isotype :

IgG

Storage :

-20°C

Description :

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

Immunogen :

Synthetic peptide corresponding to residues near the carboxy-terminus of human p21

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100-1:400, Immunocytochemistry: 1:800, Immunohistochemistry (Paraffin): 1:50, Immunoprecipitation: 1:50, Western Blot: 1:1,000

Aliases :

Activating Fragment 1; CAP20; cdk inhibitor CIP1; cdk inhibitor CIP1 (p21); CDKI; CDK-interacting protein 1; CDK-interaction protein 1; Cdkn1; CDKN1A; CDNK1A; CIP1; Cip1); cyclin dependent kinase inhibitor 1A; cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA6; MDA-6; melanoma differentiation associated protein 6; melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21 ; p21Cip1; p21Cip1/Waf1; p21WAF; PIC1; putative cyclin-dependent kinase inhibitor 1A (p21/WAF1/CIP1); SDI1; SLC12A9; UV96; WAF1; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

more info or order :

Invitrogen product webpage