Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5) | Invitrogen MA5-14918 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5)

catalog :

MA5-14918

quantity :

100 uL

price :

US 474.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

F.724.5

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 4i	Zhao P, Xu Y, Jiang L, Fan X, Ku Z, Li L, et al. LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions. Mol Neurodegener. 2022;17:44 pubmed publisher
western blot; human; 1:200; fig 1g	Hall Roberts H, Agarwal D, Obst J, Smith T, Monzón Sandoval J, Di Daniel E, et al. TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages. Alzheimers Res Ther. 2020;12:151 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 2h	Lopes A, Silva R, Fonseca M, Gomes F, Maganin A, Ribeiro L, et al. Molecular basis of carrageenan-induced cytokines production in macrophages. Cell Commun Signal. 2020;18:141 pubmed publisher
western blot; mouse; loading ...; fig 6b	Bergsma A, Ganguly S, Wiegand M, Dick D, Williams B, Miranti C. Regulation of cytoskeleton and adhesion signaling in osteoclasts by tetraspanin CD82. Bone Rep. 2019;10:100196 pubmed publisher
western blot; mouse; loading ...; fig 3f	Zhao Y, Wu X, Li X, Jiang L, Gui X, Liu Y, et al. TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function. Neuron. 2018;97:1023-1031.e7 pubmed publisher
	Birkle T, Brown G. Syk inhibitors protect against microglia-mediated neuronal loss in culture. Front Aging Neurosci. 2023;15:1120952 pubmed publisher
	Bushey R, Saxena R, Campa M, Gottlin E, He Y, Patz E. Antitumor Immune Mechanisms of the Anti-Complement Factor H Antibody GT103. Mol Cancer Ther. 2023;22:778-789 pubmed publisher
	Jung E, Suh K, Han J, Kim H, Kang H, Choi W, et al. Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway. Aging Cell. 2022;21:e13623 pubmed publisher
	Obst J, Hall Roberts H, Smith T, Kreuzer M, Magno L, Di Daniel E, et al. PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages. Sci Rep. 2021;11:19842 pubmed publisher
	Natunen T, Martiskainen H, Marttinen M, Gabbouj S, Koivisto H, Kemppainen S, et al. Diabetic phenotype in mouse and humans reduces the number of microglia around β-amyloid plaques. Mol Neurodegener. 2020;15:66 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5)

Catalog # :

MA5-14918

Quantity :

100 uL

Price :

US 474.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Human

Applications :

Flow Cytometry: 1:200-1:800, Immunocytochemistry: 1:100-1:400, Immunoprecipitation: 1:50, Western Blot: 1:1,000

Species :

Human

Clone :

F.724.5

Isotype :

IgG

Storage :

-20 C

Description :

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding pTyr525/526 of human Syk

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:200-1:800, Immunocytochemistry: 1:100-1:400, Immunoprecipitation: 1:50, Western Blot: 1:1,000

Aliases :

DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72Syk; p72-Syk; ptk72; SP1; spleen associated tyrosine kinase; spleen tyrosine kinase; SYK; syk signaling; Sykb; tyrosine-kinase syk; tyrosine-protein kinase SYK

more info or order :

Invitrogen product webpage