AGO2 Monoclonal Antibody (R.386.2) | Invitrogen MA5-14861 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

AGO2 Monoclonal Antibody (R.386.2)

catalog :

MA5-14861

quantity :

100 uL

price :

US 474.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

R.386.2

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
RNA immunoprecipitation; human; fig s11	Holdt L, Stahringer A, Sass K, Pichler G, Kulak N, Wilfert W, et al. Circular non-coding RNA ANRIL modulates ribosomal RNA maturation and atherosclerosis in humans. Nat Commun. 2016;7:12429 pubmed publisher
immunoprecipitation; mouse; fig s2	Gupta S, Itagaki R, Zheng X, Batkai S, Thum S, Ahmad F, et al. miR-21 promotes fibrosis in an acute cardiac allograft transplantation model. Cardiovasc Res. 2016;110:215-26 pubmed publisher
	Qian J, Lei X, Sun Y, Zheng L, Li J, Zhang S, et al. Long non-coding RNA SNHG8 enhances triple-negative breast cancer cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis. Biol Direct. 2021;16:13 pubmed publisher
	Xie H, Zhao J, Wan J, Zhao J, Wang Q, Yang X, et al. Long non‑coding RNA AC245100.4 promotes prostate cancer tumorigenesis via the microRNA‑145‑5p/RBBP5 axis. Oncol Rep. 2021;45:619-629 pubmed publisher
	Zhao P, Cheng J, Li B, Nie D, Wang H, Li C, et al. LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis. Cancer Cell Int. 2021;21:14 pubmed publisher
	Schimmel K, Stojanovi x107 S, Huang C, Jung M, Meyer M, Xiao K, et al. Combined high-throughput library screening and next generation RNA sequencing uncover microRNAs controlling human cardiac fibroblast biology. J Mol Cell Cardiol. 2021;150:91-100 pubmed publisher
	Mead B, Amaral J, Tomarev S. Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Promote Neuroprotection in Rodent Models of Glaucoma. Invest Ophthalmol Vis Sci. 2018;59:702-714 pubmed publisher

product information

Product Type :

Antibody

Product Name :

AGO2 Monoclonal Antibody (R.386.2)

Catalog # :

MA5-14861

Quantity :

100 uL

Price :

US 474.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunoprecipitation: 1:50, Western Blot: 1:1,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

R.386.2

Isotype :

IgG

Storage :

-20 C

Description :

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or “Slicer” activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

Immunogen :

Synthetic peptide corresponding to mouse Argonaute 2

Format :

Liquid

Applications w/Dilutions :

Immunoprecipitation: 1:50, Western Blot: 1:1,000

Aliases :

1110029L17Rik; 2310051F07Rik; AGO1; AGO2; AI225898; AL022874; argonaute 2; argonaute 2, RISC catalytic component; argonaute RISC catalytic component 2; argonaute RISC catalytic subunit 2; Argonaute1; argonaute2; argonaute2 {ECO:0000255; AW546247; CTA-204B4.6; eIF2C; eIF2C 1; eIF-2C 1; eIF2C 2; eIF-2C 2; eIF2C 2 {ECO:0000255; eIF-2C 2 {ECO:0000255; EIF2C1; Eif2c2; ENSMUSG00000072493; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255; eukaryotic translation initiation factor 2C, 2; GERp95; Gm10365; Golgi ER protein 95 kDa; hAgo1; hAgo2; HAMAP-Rule:MF_03031}; Kiaa4215; mAgo2; mKIAA4215; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-1; protein argonaute-2; Protein slicer; Q10

more info or order :

Invitrogen product webpage