KSHV ORF45 Monoclonal Antibody (2D4A5) | Invitrogen MA5-14769 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

KSHV ORF45 Monoclonal Antibody (2D4A5)

catalog :

MA5-14769

quantity :

100 uL

price :

US 504.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2D4A5

reactivity :

Human gammaherpesvirus 8, human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1e	Méndez Solís O, Bendjennat M, Naipauer J, Theodoridis P, Ho J, Verdun R, et al. Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis. Cell Rep. 2021;37:110144 pubmed publisher
western blot; human; fig 1a, 8b	Johnston B, Pringle E, McCormick C. KSHV activates unfolded protein response sensors but suppresses downstream transcriptional responses to support lytic replication. PLoS Pathog. 2019;15:e1008185 pubmed publisher
western blot; human; fig 2	Pringle E, Robinson C, McCormick C. Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Interferes with mTORC1 Regulation of Autophagy and Viral Protein Synthesis. J Virol. 2019;93: pubmed publisher
	Zhang H, Wong J, Ni G, Cano P, Dittmer D, Damania B. Mitochondrial protein, TBRG4, modulates KSHV and EBV reactivation from latency. PLoS Pathog. 2022;18:e1010990 pubmed publisher
	Kang S, Lee M, Ryu H, Lee J, Lee M. Dimethyl Sulfoxide Enhances Kaposi's Sarcoma-Associated Herpesvirus Production During Lytic Replication. Front Microbiol. 2021;12:778525 pubmed publisher
	Ni G, Ma Z, Wong J, Zhang Z, Cousins E, Major M, et al. PPP6C Negatively Regulates STING-Dependent Innate Immune Responses. MBio. 2020;11: pubmed publisher
	Zhang H, Ni G, Damania B. ADAR1 Facilitates KSHV Lytic Reactivation by Modulating the RLR-Dependent Signaling Pathway. Cell Rep. 2020;31:107564 pubmed publisher
	Hopcraft S, Pattenden S, James L, FRYE S, Dittmer D, Damania B. Chromatin remodeling controls Kaposi's sarcoma-associated herpesvirus reactivation from latency. PLoS Pathog. 2018;14:e1007267 pubmed publisher
	Ma Z, Hopcraft S, Yang F, Petrucelli A, Guo H, Ting J, et al. NLRX1 negatively modulates type I IFN to facilitate KSHV reactivation from latency. PLoS Pathog. 2017;13:e1006350 pubmed publisher

product information

Product Type :

Antibody

Product Name :

KSHV ORF45 Monoclonal Antibody (2D4A5)

Catalog # :

MA5-14769

Quantity :

100 uL

Price :

US 504.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Virus

Applications :

ELISA: 1:10,000, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Virus

Clone :

2D4A5

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.

Immunogen :

Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

Kaposi's Sarcoma Virus; Kaposi's sarcoma-associated herpesvirus

more info or order :

Invitrogen product webpage