HSF1 Monoclonal Antibody (4B4) | Invitrogen MA5-14622 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSF1 Monoclonal Antibody (4B4)

catalog :

MA5-14622

quantity :

500 uL

price :

US 474.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

4B4

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 4b	Vilaboa N, Boré A, Martin Saavedra F, Bayford M, Winfield N, Firth Clark S, et al. New inhibitor targeting human transcription factor HSF1: effects on the heat shock response and tumor cell survival. Nucleic Acids Res. 2017;45:5797-5817 pubmed publisher
western blot; mouse; 1:1000	Patel P, Julien J, Kriz J. Early-stage treatment with Withaferin A reduces levels of misfolded superoxide dismutase 1 and extends lifespan in a mouse model of amyotrophic lateral sclerosis. Neurotherapeutics. 2015;12:217-33 pubmed publisher
immunoprecipitation; human western blot; human	Logan I, McNeill H, Cook S, Lu X, Meek D, Fuller Pace F, et al. Heat shock factor-1 modulates p53 activity in the transcriptional response to DNA damage. Nucleic Acids Res. 2009;37:2962-73 pubmed publisher
immunoprecipitation; human western blot; human	Lee Y, Kim E, Lee J, Jeoung D, Bae S, Kwon S, et al. HSF1 as a mitotic regulator: phosphorylation of HSF1 by Plk1 is essential for mitotic progression. Cancer Res. 2008;68:7550-60 pubmed publisher
western blot; human	Lu M, Kim H, Li C, Kim S, Kwak I, Lee Y, et al. Two distinct disulfide bonds formed in human heat shock transcription factor 1 act in opposition to regulate its DNA binding activity. Biochemistry. 2008;47:6007-15 pubmed publisher
western blot; mouse	Lee Y, Lee H, Lee J, Jeoung D, Kang C, Bae S, et al. A novel function for HSF1-induced mitotic exit failure and genomic instability through direct interaction between HSF1 and Cdc20. Oncogene. 2008;27:2999-3009 pubmed
immunocytochemistry; human	Valgardsdottir R, Chiodi I, Giordano M, Rossi A, Bazzini S, Ghigna C, et al. Transcription of Satellite III non-coding RNAs is a general stress response in human cells. Nucleic Acids Res. 2008;36:423-34 pubmed
immunocytochemistry; human western blot; human	Valgardsdottir R, Chiodi I, Giordano M, Cobianchi F, Riva S, Biamonti G. Structural and functional characterization of noncoding repetitive RNAs transcribed in stressed human cells. Mol Biol Cell. 2005;16:2597-604 pubmed
	Lecomte S, Reverdy L, Le Quement C, Le Masson F, Amon A, Le Goff P, et al. Unraveling complex interplay between heat shock factor 1 and 2 splicing isoforms. PLoS ONE. 2013;8:e56085 pubmed publisher
	Denegri M, Moralli D, Rocchi M, Biggiogera M, Raimondi E, Cobianchi F, et al. Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies. Mol Biol Cell. 2002;13:2069-79 pubmed
	Mathew A, Mathur S, Jolly C, Fox S, Kim S, Morimoto R. Stress-specific activation and repression of heat shock factors 1 and 2. Mol Cell Biol. 2001;21:7163-71 pubmed

product information

Product Type :

Antibody

Product Name :

HSF1 Monoclonal Antibody (4B4)

Catalog # :

MA5-14622

Quantity :

500 uL

Price :

US 474.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Rat

Reactivity :

Human, Mouse, Rat

Applications :

Gel Shift: 1mg/mL, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Species :

Human, Mouse, Rat

Clone :

4B4

Isotype :

IgG1

Storage :

4 C

Description :

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

Immunogen :

Recombinant mouse HSF1 protein (aa1-503)

Format :

Liquid

Applications w/Dilutions :

Gel Shift: 1mg/mL, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Aliases :

AA960185; heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF; HSF 1; Hsf1; Hsf1alpha; Hsf1beta; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; HSTF1

more info or order :

Invitrogen product webpage