Cytokeratin 8 Monoclonal Antibody (TS1) | Invitrogen MA5-14428 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cytokeratin 8 Monoclonal Antibody (TS1)

catalog :

MA5-14428

quantity :

500 µL

price :

US 449

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TS1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig 3	Gilbert S, Loranger A, Marceau N. Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells. Mol Cell Biol. 2004;24:7072-81 pubmed
western blot; human; loading ...; fig 2e	Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, et al. Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON. Sci Rep. 2016;6:34472 pubmed publisher
immunocytochemistry; human; fig 2c western blot; human; fig 3h	Kuga T, Sasaki M, Mikami T, Miake Y, Adachi J, Shimizu M, et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Sci Rep. 2016;6:26557 pubmed publisher
immunohistochemistry; human; 1:300	BaÅŸak K, KiroÄŸlu K. Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report. Medicine (Baltimore). 2014;93:e246 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Snider N, Park H, Omary M. A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization. J Biol Chem. 2013;288:31329-37 pubmed publisher
immunocytochemistry; human western blot; human	Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, et al. A novel mechanism of keratin cytoskeleton organization through casein kinase I? and FAM83H in colorectal cancer. J Cell Sci. 2013;126:4721-31 pubmed publisher
immunoprecipitation; human	Snider N, Leonard J, Kwan R, Griggs N, Rui L, Omary M. Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol. 2013;200:241-7 pubmed publisher
western blot; human	Duan Y, Sun Y, Zhang F, Zhang W, Wang D, Wang Y, et al. Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch. J Biol Chem. 2012;287:40547-59 pubmed publisher
immunohistochemistry; human western blot; human	Létourneau I, Quinn M, Wang L, Portelance L, Caceres K, Cyr L, et al. Derivation and characterization of matched cell lines from primary and recurrent serous ovarian cancer. BMC Cancer. 2012;12:379 pubmed publisher
immunohistochemistry; human; 1:100	Barak S, Wang Z, Miettinen M. Immunoreactivity for calretinin and keratins in desmoid fibromatosis and other myofibroblastic tumors: a diagnostic pitfall. Am J Surg Pathol. 2012;36:1404-9 pubmed publisher
western blot; mouse	Hanada S, Harada M, Abe M, Akiba J, Sakata M, Kwan R, et al. Aging modulates susceptibility to mouse liver Mallory-Denk body formation. J Histochem Cytochem. 2012;60:475-83 pubmed publisher
western blot; mouse	Singla A, Moons D, Snider N, Wagenmaker E, Jayasundera V, Omary M. Oxidative stress, Nrf2 and keratin up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria. Hepatology. 2012;56:322-31 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Chang H, Chen B, Wu C, Tsao Z, Chen Y, Chang C, et al. Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model. J Biomed Sci. 2011;18:6 pubmed publisher
immunocytochemistry; mouse; 1:200	Snider N, Weerasinghe S, INIGUEZ LLUHI J, Herrmann H, Omary M. Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation. J Biol Chem. 2011;286:2273-84 pubmed publisher
immunohistochemistry; human	Carvalho L, Pereira E, Frappart L, Boniol M, Bernardo W, Tarricone V, et al. Molecular characterization of breast cancer in young Brazilian women. Rev Assoc Med Bras (1992). 2010;56:278-87 pubmed
immunocytochemistry; mouse western blot; mouse	Gilbert S, Ruel A, Loranger A, Marceau N. Switch in Fas-activated death signaling pathway as result of keratin 8/18-intermediate filament loss. Apoptosis. 2008;13:1479-93 pubmed publisher
immunohistochemistry; human western blot; human	Ouellet V, Zietarska M, Portelance L, Lafontaine J, Madore J, Puiffe M, et al. Characterization of three new serous epithelial ovarian cancer cell lines. BMC Cancer. 2008;8:152 pubmed publisher
western blot; human; 1:200; fig 6	Page J, Johnson M, Olsavsky K, Strom S, Zarbl H, Omiecinski C. Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci. 2007;97:384-97 pubmed
immunocytochemistry; human western blot; human	Tao G, Nakamichi I, Ku N, Wang J, Frolkis M, Gong X, et al. Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. Exp Cell Res. 2006;312:411-22 pubmed
immunocytochemistry; human; 1:300 western blot; human	Scott G, Atsriku C, Kaminker P, Held J, Gibson B, Baldwin M, et al. Vitamin K3 (menadione)-induced oncosis associated with keratin 8 phosphorylation and histone H3 arylation. Mol Pharmacol. 2005;68:606-15 pubmed
	Peculis R, Mandrika I, Petrovska R, Dortane R, Megnis K, Nazarovs J, et al. Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue. Front Endocrinol (Lausanne). 2020;11:313 pubmed publisher
	Pu T, Xiong L, Liu Q, Zhang M, Cai Q, Liu H, et al. Delineation of retroperitoneal metastatic lymph nodes in ovarian cancer with near-infrared fluorescence imaging. Oncol Lett. 2017;14:2869-2877 pubmed publisher
	Maitra D, Elenbaas J, Whitesall S, Basrur V, D Alecy L, Omary M. Ambient Light Promotes Selective Subcellular Proteotoxicity after Endogenous and Exogenous Porphyrinogenic Stress. J Biol Chem. 2015;290:23711-24 pubmed publisher
	Sørensen C, Andresen B, Jensen U, Jensen T, Jensen P, Gregersen N, et al. Functional testing of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex. Exp Dermatol. 2003;12:472-9 pubmed

product information

Product Type :

Antibody

Product Name :

Cytokeratin 8 Monoclonal Antibody (TS1)

Catalog # :

MA5-14428

Quantity :

500 µL

Price :

US 449

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 1:50, Western Blot: 1:25-1:50

Species :

Human

Clone :

TS1

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Immunogen :

Keratin preparation from a human carcinoma

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 1:50, Western Blot: 1:25-1:50

Aliases :

AA960620; AL022697; AU019895; C CY K2C8; CARD2; cb186; CK 8; CK8; CK-8; CYK8; CYKER; cytokeratin 8; cytokeratin 8 (370 AA); Cytokeratin endo A; cytokeratin-8; Cytokeratin-A; DreK8; EndoA; etID33550.23; etID43142.23; etID43142.8; hard keratin; K2C8; K8; K-8; KERA; keratin 8; keratin 8, type II; keratin complex 2, basic, gene 8; keratin, type 2, gene 8; keratin, type II cytoskeletal 8; Keratin-8; KO; Krt-2.8; krt2-8; KRT8; LOW QUALITY PROTEIN: keratin, type II cytoskeletal 8; sb:cb186; type II cytokeratin A (no.8); type-II keratin Kb8; wu:fa20h05; wu:fa95h10; wu:fb96c06; zfk8; zf-K8