RAD51 Monoclonal Antibody (51RAD01 (3C10)) | Invitrogen MA5-14419 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

RAD51 Monoclonal Antibody (51RAD01 (3C10))

catalog :

MA5-14419

quantity :

500 uL

price :

US 480.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

51RAD01 (3C10)

reactivity :

Chlamydomonas reinhardtii, Rhynchospora tenuis, Rhynchospora pubera, Tetrahymena thermophila, fission yeast, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; loading ...; fig 5c	Bloniarz D, Adamczyk Grochala J, Lewinska A, Wnuk M. The lack of functional DNMT2/TRDMT1 gene modulates cancer cell responses during drug-induced senescence. Aging (Albany NY). 2021;13:15833-15874 pubmed publisher
western blot; mouse; 1:200; fig 2a	Atkins A, Xu M, Li M, Rogers N, Pryzhkova M, Jordan P. SMC5/6 is required for replication fork stability and faithful chromosome segregation during neurogenesis. elife. 2020;9: pubmed publisher
immunohistochemistry - paraffin section; Tetrahymena thermophila; 1:50; loading ...; fig 2a	Tian M, Loidl J. A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation. Nucleic Acids Res. 2018;46:11822-11834 pubmed publisher
western blot; human; 1:200; loading ...; fig 4b	Skvarova Kramarzova K, Osborn M, Webber B, Defeo A, McElroy A, Kim C, et al. CRISPR/Cas9-Mediated Correction of the FANCD1 Gene in Primary Patient Cells. Int J Mol Sci. 2017;18: pubmed publisher
immunocytochemistry; Tetrahymena thermophila; loading ...	Shodhan A, Kataoka K, Mochizuki K, Novatchkova M, Loidl J. A Zip3-like protein plays a role in crossover formation in the SC-less meiosis of the protist Tetrahymena. Mol Biol Cell. 2017;28:825-833 pubmed publisher
western blot; human; loading ...; fig 3	Ishiguro K, Zhu Y, Lin Z, Penketh P, Shyam K, Zhu R, et al. Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines. J Transl Sci. 2016;2:117-124 pubmed
immunohistochemistry - paraffin section; human; 1:100; fig 6 immunocytochemistry; human; 1:100; fig 5 western blot; human; 1:500; fig 5	Annovazzi L, Caldera V, Mellai M, Riganti C, Battaglia L, Chirio D, et al. The DNA damage/repair cascade in glioblastoma cell lines after chemotherapeutic agent treatment. Int J Oncol. 2015;46:2299-308 pubmed publisher
immunohistochemistry; Rhynchospora tenuis; 1:75; fig s1 immunohistochemistry; Rhynchospora pubera	Cabral G, Marques A, Schubert V, Pedrosa Harand A, SchlÃ¶gelhofer P. Chiasmatic and achiasmatic inverted meiosis of plants with holocentric chromosomes. Nat Commun. 2014;5:5070 pubmed publisher
immunohistochemistry; human; 1:100	Nakanoko T, Saeki H, Morita M, Nakashima Y, Ando K, Oki E, et al. Rad51 expression is a useful predictive factor for the efficacy of neoadjuvant chemoradiotherapy in squamous cell carcinoma of the esophagus. Ann Surg Oncol. 2014;21:597-604 pubmed publisher
immunohistochemistry; human; 1:75	Mattila R, Rautava J, SyrjÃ¤nen S. Human papillomavirus in oral atrophic lichen planus lesions. Oral Oncol. 2012;48:980-984 pubmed publisher
immunocytochemistry; human	Hsu J, Lin T, Tsai R. Nucleostemin prevents telomere damage by promoting PML-IV recruitment to SUMOylated TRF1. J Cell Biol. 2012;197:613-24 pubmed publisher
immunocytochemistry; human; 1:50 western blot; human; 1:200	Howard Till R, Lukaszewicz A, Loidl J. The recombinases Rad51 and Dmc1 play distinct roles in DNA break repair and recombination partner choice in the meiosis of Tetrahymena. PLoS Genet. 2011;7:e1001359 pubmed publisher
immunohistochemistry; mouse western blot; mouse	Lin Y, Sengupta S, Gurdziel K, Bell G, Jacks T, Flores E. p63 and p73 transcriptionally regulate genes involved in DNA repair. PLoS Genet. 2009;5:e1000680 pubmed publisher
immunocytochemistry; human; 1:100	Erenpreisa J, Cragg M, Salmina K, Hausmann M, Scherthan H. The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells. Exp Cell Res. 2009;315:2593-603 pubmed publisher
immunocytochemistry; Chlamydomonas reinhardtii; 1:50	Mochizuki K, Novatchkova M, Loidl J. DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. J Cell Sci. 2008;121:2148-58 pubmed publisher
immunoprecipitation; human	Fischer J, Lancia J, Mathur A, Smith M. Selenium protection from DNA damage involves a Ref1/p53/Brca1 protein complex. Anticancer Res. 2006;26:899-904 pubmed
immunocytochemistry; fission yeast; 1:50	Lorenz A, Estreicher A, Kohli J, Loidl J. Meiotic recombination proteins localize to linear elements in Schizosaccharomyces pombe. Chromosoma. 2006;115:330-40 pubmed
western blot; human	Dutta A, Chakraborty A, Saha A, Ray S, Chatterjee A. Interaction of radiation- and bleomycin-induced lesions and influence of glutathione level on the interaction. Mutagenesis. 2005;20:329-35 pubmed
western blot; human	Chinnaiyan P, Huang S, Vallabhaneni G, Armstrong E, Varambally S, Tomlins S, et al. Mechanisms of enhanced radiation response following epidermal growth factor receptor signaling inhibition by erlotinib (Tarceva). Cancer Res. 2005;65:3328-35 pubmed
	Singh A, Kumar S, Beniwal R, Mohanty A, Kushwaha B, Rao H. Local DNA synthesis is critical for DNA repair during oocyte maturation. J Cell Sci. 2021;134: pubmed publisher
	Bondarieva A, Raveendran K, Telychko V, Rao H, Ravindranathan R, Zorzompokou C, et al. Proline-rich protein PRR19 functions with cyclin-like CNTD1 to promote meiotic crossing over in mouse. Nat Commun. 2020;11:3101 pubmed publisher
	El Bezawy R, Tripari M, Percio S, Cicchetti A, Tortoreto M, Stucchi C, et al. SPOP Deregulation Improves the Radiation Response of Prostate Cancer Models by Impairing DNA Damage Repair. Cancers (Basel). 2020;12: pubmed publisher
	Mi Y, Dong H, Sun X, Ren F, Tang Y, Zheng P. The association of Helicobacter pylori CagA EPIYA motifs and vacA genotypes with homologous recombination repair markers during the gastric precancerous cascade. Int J Biol Markers. 2020;35:49-55 pubmed publisher
	Leonardi S, Buttarelli M, De Stefano I, Ferrandina G, Petrillo M, Babini G, et al. The relevance of prelamin A and RAD51 as molecular biomarkers in cervical cancer. Oncotarget. 2017;8:94247-94258 pubmed publisher
	Tanaka N, Patel A, Tang L, Silver N, Lindemann A, Takahashi H, et al. Replication Stress Leading to Apoptosis within the S-phase Contributes to Synergism between Vorinostat and AZD1775 in HNSCC Harboring High-Risk TP53 Mutation. Clin Cancer Res. 2017;23:6541-6554 pubmed publisher
	Howard Till R, Lukaszewicz A, Novatchkova M, Loidl J. A single cohesin complex performs mitotic and meiotic functions in the protist tetrahymena. PLoS Genet. 2013;9:e1003418 pubmed publisher
	Loidl J, Lukaszewicz A, Howard Till R, Koestler T. The Tetrahymena meiotic chromosome bouquet is organized by centromeres and promotes interhomolog recombination. J Cell Sci. 2012;125:5873-80 pubmed publisher
	McCabe N, Turner N, Lord C, Kluzek K, Bialkowska A, Swift S, et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res. 2006;66:8109-15 pubmed
	Loidl J, Scherthan H. Organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila. J Cell Sci. 2004;117:5791-801 pubmed
	Wilkinson D, Weller S. Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage respon. J Virol. 2004;78:4783-96 pubmed
	Ivanov A, Cragg M, Erenpreisa J, Emzinsh D, Lukman H, Illidge T. Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks. J Cell Sci. 2003;116:4095-106 pubmed
	Sarkisian C, Master S, Huber L, Ha S, Chodosh L. Analysis of murine Brca2 reveals conservation of protein-protein interactions but differences in nuclear localization signals. J Biol Chem. 2001;276:37640-8 pubmed

product information

Product Type :

Antibody

Product Name :

RAD51 Monoclonal Antibody (51RAD01 (3C10))

Catalog # :

MA5-14419

Quantity :

500 uL

Price :

US 480.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 2-4 ug/mL, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Species :

Human, Mouse, Rat

Clone :

51RAD01 (3C10)

Isotype :

IgG1, kappa

Storage :

4 C

Description :

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

Immunogen :

Recombinant Rad51 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 2-4 ug/mL, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Aliases :

AV304093; BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51; DNA repair protein RAD51 homolog 1; DNA repair protein RAD51 homolog A; DNA repair protein rhp51; FANCR; homolog to S.cerevisiae; HRAD51; HsRad51; HsT16930; MGC128559 protein; MRMV2; MUT5; rad51; RAD51 homolog; RAD51 homolog (RecA homolog); RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RAD51 recombinase; RAD51 recombinase L homeolog; rad51.1; rad51.L; rad51-1; RAD51A; rad51-a; rad51-b; Reca; RecA family recombinase Rhp51; RecA, E. coli, homolog of; RecA-like protein; recombinase RAD51; recombination protein A; RGD1563603; rhp51; SPAC644.14c; wu:fb38e12; xrad51; XRad51.1; YER095W; zgc:77754

more info or order :

Invitrogen product webpage