E2F1 Monoclonal Antibody (KH95), Biotin | Invitrogen MA5-14341 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

E2F1 Monoclonal Antibody (KH95), Biotin

catalog :

MA5-14341

quantity :

500µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

KH95

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human	McClendon A, Dean J, Rivadeneira D, Yu J, Reed C, Gao E, et al. CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy. Cell Cycle. 2012;11:2747-55 pubmed publisher
immunohistochemistry; human; 1:100	Xanthoulis A, Kotsinas A, Tiniakos D, Fiska A, Tentes A, Kyroudi A, et al. The relationship between E2F family members and tumor growth in colorectal adenocarcinomas: A comparative immunohistochemical study of 100 cases. Appl Immunohistochem Mol Morphol. 2014;22:471-7 pubmed publisher
immunohistochemistry; human; 1:250	Vékony H, Roser K, Löning T, Raaphorst F, Leemans C, Van der Waal I, et al. Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands. Histopathology. 2008;53:658-66 pubmed publisher
western blot; human	Bromberg Z, Raj N, Goloubinoff P, Deutschman C, Weiss Y. Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome. Crit Care Med. 2008;36:246-55 pubmed
	Au N, Cheang M, Huntsman D, Yorida E, Coldman A, Elliott W, et al. Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers. J Pathol. 2004;204:101-9 pubmed
	Mady H, Hasso S, Melhem M. Expression of E2F-4 gene in colorectal adenocarcinoma and corresponding covering mucosa: an immunohistochemistry, image analysis, and immunoblot study. Appl Immunohistochem Mol Morphol. 2002;10:225-30 pubmed
	Hsueh C, Wu Y, Schwartz G. UCN-01 suppresses E2F-1 mediated by ubiquitin-proteasome-dependent degradation. Clin Cancer Res. 2001;7:669-74 pubmed

product information

Product Type :

Antibody

Product Name :

E2F1 Monoclonal Antibody (KH95), Biotin

Catalog # :

MA5-14341

Quantity :

500µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Gel Shift: 1mg/mL, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 1:50-100, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Mouse

Clone :

KH95

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

E2F's are DNA-binding proteins that associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 also requires DP-1 for efficient DNA-binding and transcription modification. E2F1 is proposed to be involved in several cellular processes that range from tumor suppression, cell cycle progression, and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis. These observations place E2F among a group of apoptosis inducers that includes; c-Myc, Adenovirus E1A, and HPV E7 protein.

Immunogen :

Recombinant human E2F-1 protein

Format :

Liquid

Applications w/Dilutions :

Gel Shift: 1mg/mL, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 1:50-100, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3, transcription factor E2F1