Cyclin E Monoclonal Antibody (HE12) | Invitrogen MA5-14336 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin E Monoclonal Antibody (HE12)

catalog :

MA5-14336

quantity :

500 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HE12

reactivity :

African green monkey, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 35

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 4b	Pozner A, Terooatea T, Buck Koehntop B. Cell-specific Kaiso (ZBTB33) Regulation of Cell Cycle through Cyclin D1 and Cyclin E1. J Biol Chem. 2016;291:24538-24550 pubmed
immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 2	Duchnowska R, Wysocki P, Korski K, Czartoryska ArÅ‚ukowicz B, NiwiÅ„ska A, Orlikowska M, et al. Immunohistochemical prediction of lapatinib efficacy in advanced HER2-positive breast cancer patients. Oncotarget. 2016;7:550-64 pubmed publisher
western blot; human	Bisteau X, Paternot S, Colleoni B, Ecker K, Coulonval K, De Groote P, et al. CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point. PLoS Genet. 2013;9:e1003546 pubmed publisher
immunocytochemistry; human	Suzuki M, Yamauchi M, Oka Y, Suzuki K, Yamashita S. Live-cell imaging visualizes frequent mitotic skipping during senescence-like growth arrest in mammary carcinoma cells exposed to ionizing radiation. Int J Radiat Oncol Biol Phys. 2012;83:e241-50 pubmed publisher
western blot; human; 0.5 ug/ml; fig s2	Lofmark S, de Klerk N, Aro H. Neisseria gonorrhoeae infection induces altered amphiregulin processing and release. PLoS ONE. 2011;6:e16369 pubmed publisher
western blot; human; 1:200; fig 6e	Rico Bautista E, Yang C, Lu L, Roth G, Wolf D. Chemical genetics approach to restoring p27Kip1 reveals novel compounds with antiproliferative activity in prostate cancer cells. BMC Biol. 2010;8:153 pubmed publisher
western blot; mouse	Cimica V, Smith M, Zhang Z, Mathur D, Mani S, Kalpana G. Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen. BMC Cancer. 2010;10:634 pubmed publisher
western blot; human	Holubcová Z, Matula P, Sedláčková M, Vinarsky V, Dolezalova D, Bárta T, et al. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011;29:46-56 pubmed publisher
western blot; human	Bárta T, Vinarsky V, Holubcová Z, Dolezalova D, Verner J, Pospisilova S, et al. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010;28:1143-52 pubmed publisher
western blot; human; 1 ug/ml; fig 2	Chimploy K, Díaz G, Li Q, Carter O, Dashwood W, Mathews C, et al. E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin. Int J Cancer. 2009;125:2086-94 pubmed publisher
immunohistochemistry; human	Martin D, Boersma B, Yi M, Reimers M, Howe T, Yfantis H, et al. Differences in the tumor microenvironment between African-American and European-American breast cancer patients. PLoS ONE. 2009;4:e4531 pubmed publisher
western blot; human	Cheng T, Teng C, Tsai W, Yeh C, Wu M, Hsu H, et al. Multitarget therapy of malignant cancers by the head-to-tail tandem array multiple shRNAs expression system. Cancer Gene Ther. 2009;16:516-31 pubmed publisher
western blot; human	Hudson T, Hartle D, Hursting S, Nunez N, Wang T, Young H, et al. Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms. Cancer Res. 2007;67:8396-405 pubmed
western blot; human	Al Ayyoubi S, Gali Muhtasib H. Differential apoptosis by gallotannin in human colon cancer cells with distinct p53 status. Mol Carcinog. 2007;46:176-86 pubmed
western blot; human; 2.6 ug/ml; fig 4	Jones A, Jonsson A, Aro H. Neisseria gonorrhoeae infection causes a G1 arrest in human epithelial cells. FASEB J. 2007;21:345-55 pubmed
western blot; human; 1:500	Wiseman D, Werner S, Crowell P. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. J Pharmacol Exp Ther. 2007;320:1163-70 pubmed
immunohistochemistry; human; 1:100	Kliman H, Honig S, Walls D, Luna M, McSweet J, Copperman A. Optimization of endometrial preparation results in a normal endometrial function test (EFT) and good reproductive outcome in donor ovum recipients. J Assist Reprod Genet. 2006;23:299-303 pubmed
immunohistochemistry; human	Boersma B, Howe T, Goodman J, Yfantis H, Lee D, Chanock S, et al. Association of breast cancer outcome with status of p53 and MDM2 SNP309. J Natl Cancer Inst. 2006;98:911-9 pubmed
western blot; African green monkey western blot; human	Yang X, Liu J, Ye L, Liao Q, Wu J, Gao J, et al. HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression. Virus Res. 2006;121:134-43 pubmed
western blot; human	Siitonen T, Koistinen P, Savolainen E. Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells. Leuk Res. 2005;29:1335-42 pubmed
western blot; human; 1:200	Lu L, Schulz H, Wolf D. The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biol. 2002;3:22 pubmed
	Silconi Z, Rosic V, Benazic S, Radosavljevic G, Mijajlovic M, Pantic J, et al. The Pt(S-pr-thiosal)2 and BCL1 Leukemia Lymphoma: Antitumor Activity In Vitro and In Vivo. Int J Mol Sci. 2022;23: pubmed publisher
	Bai X, Zhou Y, Lin Q, Huang C, Zhang S, Carlson R, et al. Bio-nanoparticle based therapeutic vaccine induces immunogenic response against triple negative breast cancer. Am J Cancer Res. 2021;11:4141-4174 pubmed
	Bajzikova M, Kovarova J, Coelho A, Boukalova S, Oh S, Rohlenova K, et al. Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells. Cell Metab. 2019;29:399-416.e10 pubmed publisher
	Jurisevic M, Arsenijević A, Pantic J, Gajovic N, Milovanovic J, Milovanovic M, et al. The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis. Oncotarget. 2018;9:28195-28212 pubmed publisher
	Wang L, Tu Z, Liu C, Liu H, Kaldis P, Chen Z, et al. Dual roles of TRF1 in tethering telomeres to the nuclear envelope and protecting them from fusion during meiosis. Cell Death Differ. 2018;25:1174-1188 pubmed publisher
	Tadokoro T, Fujihara S, Chiyo T, Oura K, Samukawa E, Yamana Y, et al. Induction of apoptosis by Galectin-9 in liver metastatic cancer cells: In vitro study. Int J Oncol. 2017;51:607-614 pubmed publisher
	Yang C, Fazli L, Loguercio S, Zharkikh I, Aza Blanc P, Gleave M, et al. Downregulation of c-SRC kinase CSK promotes castration resistant prostate cancer and pinpoints a novel disease subclass. Oncotarget. 2015;6:22060-71 pubmed
	Drayton S, Rowe J, Jones R, Vatcheva R, Cuthbert Heavens D, Marshall J, et al. Tumor suppressor p16INK4a determines sensitivity of human cells to transformation by cooperating cellular oncogenes. Cancer Cell. 2003;4:301-10 pubmed
	Joaquin M, Watson R. The cell cycle-regulated B-Myb transcription factor overcomes cyclin-dependent kinase inhibitory activity of p57(KIP2) by interacting with its cyclin-binding domain. J Biol Chem. 2003;278:44255-64 pubmed
	Dubowy R, Feinberg R, Keefe D, Doncel G, Williams S, McSweet J, et al. Improved endometrial assessment using cyclin E and p27. Fertil Steril. 2003;80:146-56 pubmed
	Murillo G, Salti G, Kosmeder J, Pezzuto J, Mehta R. Deguelin inhibits the growth of colon cancer cells through the induction of apoptosis and cell cycle arrest. Eur J Cancer. 2002;38:2446-54 pubmed
	Lewis J, Thomas T, Klinge C, Gallo M, Thomas T. Regulation of cell cycle and cyclins by 16alpha-hydroxyestrone in MCF-7 breast cancer cells. J Mol Endocrinol. 2001;27:293-307 pubmed
	Hennigan R, Stambrook P. Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes. Mol Biol Cell. 2001;12:2352-63 pubmed
	Richter J, Wagner U, Kononen J, Fijan A, Bruderer J, Schmid U, et al. High-throughput tissue microarray analysis of cyclin E gene amplification and overexpression in urinary bladder cancer. Am J Pathol. 2000;157:787-94 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin E Monoclonal Antibody (HE12)

Catalog # :

MA5-14336

Quantity :

500 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 1-2 µg/test, Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Mouse

Clone :

HE12

Isotype :

IgG1

Storage :

4° C

Description :

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Immunogen :

Recombinant human cyclin E protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1-2 µg/test, Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

AW538188; Ccne; Ccne1; CycE1; CYCLE; cyclin E; cyclin E1; cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1; pCCNE1