Cyclin E Monoclonal Antibody (HE12), Biotin | Invitrogen MA5-14333 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin E Monoclonal Antibody (HE12), Biotin

catalog :

MA5-14333

quantity :

500µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

HE12

reactivity :

African green monkey, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; human	Bisteau X, Paternot S, Colleoni B, Ecker K, Coulonval K, De Groote P, et al. CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point. PLoS Genet. 2013;9:e1003546 pubmed publisher
immunocytochemistry; human	Suzuki M, Yamauchi M, Oka Y, Suzuki K, Yamashita S. Live-cell imaging visualizes frequent mitotic skipping during senescence-like growth arrest in mammary carcinoma cells exposed to ionizing radiation. Int J Radiat Oncol Biol Phys. 2012;83:e241-50 pubmed publisher
western blot; mouse	Cimica V, Smith M, Zhang Z, Mathur D, Mani S, Kalpana G. Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen. BMC Cancer. 2010;10:634 pubmed publisher
western blot; human	Holubcová Z, Matula P, Sedláčková M, Vinarsky V, Dolezalova D, Bárta T, et al. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011;29:46-56 pubmed publisher
western blot; human	Bárta T, Vinarsky V, Holubcová Z, Dolezalova D, Verner J, Pospisilova S, et al. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010;28:1143-52 pubmed publisher
immunohistochemistry; human	Martin D, Boersma B, Yi M, Reimers M, Howe T, Yfantis H, et al. Differences in the tumor microenvironment between African-American and European-American breast cancer patients. PLoS ONE. 2009;4:e4531 pubmed publisher
western blot; human	Cheng T, Teng C, Tsai W, Yeh C, Wu M, Hsu H, et al. Multitarget therapy of malignant cancers by the head-to-tail tandem array multiple shRNAs expression system. Cancer Gene Ther. 2009;16:516-31 pubmed publisher
western blot; human	Hudson T, Hartle D, Hursting S, Nunez N, Wang T, Young H, et al. Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms. Cancer Res. 2007;67:8396-405 pubmed
western blot; human; 1:500	Wiseman D, Werner S, Crowell P. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. J Pharmacol Exp Ther. 2007;320:1163-70 pubmed
immunohistochemistry; human; 1:100	Kliman H, Honig S, Walls D, Luna M, McSweet J, Copperman A. Optimization of endometrial preparation results in a normal endometrial function test (EFT) and good reproductive outcome in donor ovum recipients. J Assist Reprod Genet. 2006;23:299-303 pubmed
immunohistochemistry; human	Boersma B, Howe T, Goodman J, Yfantis H, Lee D, Chanock S, et al. Association of breast cancer outcome with status of p53 and MDM2 SNP309. J Natl Cancer Inst. 2006;98:911-9 pubmed
western blot; African green monkey western blot; human	Yang X, Liu J, Ye L, Liao Q, Wu J, Gao J, et al. HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression. Virus Res. 2006;121:134-43 pubmed
western blot; human	Siitonen T, Koistinen P, Savolainen E. Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells. Leuk Res. 2005;29:1335-42 pubmed
western blot; human; 1:200	Lu L, Schulz H, Wolf D. The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biol. 2002;3:22 pubmed
	Drayton S, Rowe J, Jones R, Vatcheva R, Cuthbert Heavens D, Marshall J, et al. Tumor suppressor p16INK4a determines sensitivity of human cells to transformation by cooperating cellular oncogenes. Cancer Cell. 2003;4:301-10 pubmed
	Joaquin M, Watson R. The cell cycle-regulated B-Myb transcription factor overcomes cyclin-dependent kinase inhibitory activity of p57(KIP2) by interacting with its cyclin-binding domain. J Biol Chem. 2003;278:44255-64 pubmed
	Dubowy R, Feinberg R, Keefe D, Doncel G, Williams S, McSweet J, et al. Improved endometrial assessment using cyclin E and p27. Fertil Steril. 2003;80:146-56 pubmed
	Murillo G, Salti G, Kosmeder J, Pezzuto J, Mehta R. Deguelin inhibits the growth of colon cancer cells through the induction of apoptosis and cell cycle arrest. Eur J Cancer. 2002;38:2446-54 pubmed
	Lewis J, Thomas T, Klinge C, Gallo M, Thomas T. Regulation of cell cycle and cyclins by 16alpha-hydroxyestrone in MCF-7 breast cancer cells. J Mol Endocrinol. 2001;27:293-307 pubmed
	Hennigan R, Stambrook P. Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes. Mol Biol Cell. 2001;12:2352-63 pubmed
	Richter J, Wagner U, Kononen J, Fijan A, Bruderer J, Schmid U, et al. High-throughput tissue microarray analysis of cyclin E gene amplification and overexpression in urinary bladder cancer. Am J Pathol. 2000;157:787-94 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin E Monoclonal Antibody (HE12), Biotin

Catalog # :

MA5-14333

Quantity :

500µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Mouse

Clone :

HE12

Isotype :

IgG1

Storage :

4° C

Description :

Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Immunogen :

Recombinant human cyclin E protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry (Paraffin): 2-4 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

G1/S-specific cyclin-E1, cyclin Es, cyclin Et