Cyclin B1 Monoclonal Antibody (GNS11) | Invitrogen MA5-14327 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin B1 Monoclonal Antibody (GNS11)

catalog :

MA5-14327

quantity :

500 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GNS11

reactivity :

Chinese hamsters, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; fig 2a western blot; human; fig 3	Steingruber M, Kraut A, Socher E, Sticht H, Reichel A, Stamminger T, et al. Proteomic Interaction Patterns between Human Cyclins, the Cyclin-Dependent Kinase Ortholog pUL97 and Additional Cytomegalovirus Proteins. Viruses. 2016;8: pubmed publisher
western blot; human; loading ...; fig 6a	Cao L, Zhang L, Zhao X, Zhang Y. A Hybrid Chalcone Combining the Trimethoxyphenyl and Isatinyl Groups Targets Multiple Oncogenic Proteins and Pathways in Hepatocellular Carcinoma Cells. PLoS ONE. 2016;11:e0161025 pubmed publisher
immunocytochemistry; human; fig 1 western blot; human; fig 1	Steingruber M, Socher E, Hutterer C, Webel R, Bergbrede T, Lenac T, et al. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain. Viruses. 2015;7:4582-601 pubmed publisher
western blot; human; 1:200	Cattoretti G. MYC expression and distribution in normal mature lymphoid cells. J Pathol. 2013;229:430-40 pubmed publisher
immunohistochemistry; mouse	Pacal M, Bremner R. Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neurons. Dev Dyn. 2012;241:1525-44 pubmed publisher
immunohistochemistry; human; 1:100	Nar A, Ozen O, Tutuncu N, Demirhan B. Cyclin A and cyclin B1 overexpression in differentiated thyroid carcinoma. Med Oncol. 2012;29:294-300 pubmed publisher
immunohistochemistry - paraffin section; human immunohistochemistry; human; 1:50	Yoo J, Jung J, Lee M, Seo K, Shim B, Kim S, et al. Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis. J Korean Med Sci. 2007;22:318-25 pubmed
immunoprecipitation; human	Davy C, Jackson D, Raj K, Peh W, Southern S, Das P, et al. Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes. J Virol. 2005;79:3998-4011 pubmed publisher
	Su Y, Xiao L, Huang S, Wu C, Chang L, Chen Y, et al. Inhibiting WEE1 Augments the Antitumor Efficacy of Cisplatin in Urothelial Carcinoma by Enhancing the DNA Damage Process. Cells. 2023;12: pubmed publisher
	Zonderland G, Vanzo R, Gadi S, Mart xed n Doncel E, Coscia F, Mund A, et al. The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. Mol Cell. 2022;82:3350-3365.e7 pubmed publisher
	Rata S, Suarez Peredo Rodriguez M, Joseph S, Peter N, Echegaray Iturra F, Yang F, et al. Two Interlinked Bistable Switches Govern Mitotic Control in Mammalian Cells. Curr Biol. 2018;28:3824-3832.e6 pubmed publisher
	Petermann A, Pippin J, Hiromura K, Monkawa T, Durvasula R, Couser W, et al. Mitotic cell cycle proteins increase in podocytes despite lack of proliferation. Kidney Int. 2003;63:113-22 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin B1 Monoclonal Antibody (GNS11)

Catalog # :

MA5-14327

Quantity :

500 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Hamster, Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 2-4 ug/mL, Immunoprecipitation: 2 ug/mL, Western Blot: 1:100-1:1,000

Species :

Hamster, Human, Mouse, Rat

Clone :

GNS11

Isotype :

IgG2a

Storage :

4 C

Description :

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

Immunogen :

Human cyclin B1 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:10-1:200, Immunohistochemistry (Paraffin): 2-4 ug/mL, Immunoprecipitation: 2 ug/mL, Western Blot: 1:100-1:1,000

Aliases :

Ccn-2; CCNB; Ccnb1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb1; Cycb1-rs1; Cycb-4; Cycb-5; cyclin B; cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; I79_013589; OTTHUMP00000221981; OTTHUMP00000223023

more info or order :

Invitrogen product webpage