Cyclin B1 Monoclonal Antibody (GNS1) | Invitrogen MA5-14319 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin B1 Monoclonal Antibody (GNS1)

catalog :

MA5-14319

quantity :

500 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GNS1

reactivity :

Chinese hamsters, human, mouse, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 2	Liu Y, Liu C, Chang Z, Wadas B, Brower C, Song Z, et al. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J Biol Chem. 2016;291:7426-38 pubmed publisher
immunocytochemistry; human; 1:200 western blot; human; 1:10,000	Szeto S, Williams E, Rudner A, Lee J. Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation. Exp Cell Res. 2015;330:248-66 pubmed publisher
immunoprecipitation; mouse	Tane S, Kubota M, Okayama H, Ikenishi A, Yoshitome S, Iwamoto N, et al. Repression of cyclin D1 expression is necessary for the maintenance of cell cycle exit in adult mammalian cardiomyocytes. J Biol Chem. 2014;289:18033-44 pubmed publisher
western blot; human	Caffarelli N, Fehr A, Yu D. Cyclin A degradation by primate cytomegalovirus protein pUL21a counters its innate restriction of virus replication. PLoS Pathog. 2013;9:e1003825 pubmed publisher
western blot; human	Kollar P, Bárta T, Hosek J, Soucek K, Závalová V, Artinian S, et al. Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response. Evid Based Complement Alternat Med. 2013;2013:350519 pubmed publisher
immunoprecipitation; mouse	Ikenishi A, Okayama H, Iwamoto N, Yoshitome S, Tane S, Nakamura K, et al. Cell cycle regulation in mouse heart during embryonic and postnatal stages. Dev Growth Differ. 2012;54:731-8 pubmed publisher
western blot; human	Fehr A, Gualberto N, Savaryn J, Terhune S, Yu D. Proteasome-dependent disruption of the E3 ubiquitin ligase anaphase-promoting complex by HCMV protein pUL21a. PLoS Pathog. 2012;8:e1002789 pubmed publisher
western blot; mouse; fig 1	Chen S, Fu S, Hsu S, Huang Y, Hsu B. Synergistic Effect of Hyperglycemia and p27(kip1) Suppression on Adult Mouse Islet Beta Cell Replication. Int J Endocrinol. 2012;2012:417390 pubmed publisher
western blot; human	Horiuchi D, Huskey N, Kusdra L, Wohlbold L, Merrick K, Zhang C, et al. Chemical-genetic analysis of cyclin dependent kinase 2 function reveals an important role in cellular transformation by multiple oncogenic pathways. Proc Natl Acad Sci U S A. 2012;109:E1019-27 pubmed publisher
western blot; human; 1:50	Gilley R, Lochhead P, Balmanno K, Oxley D, Clark J, Cook S. CDK1, not ERK1/2 or ERK5, is required for mitotic phosphorylation of BIMEL. Cell Signal. 2012;24:170-80 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Coulonval K, Kooken H, Roger P. Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation. Mol Biol Cell. 2011;22:3971-85 pubmed publisher
immunocytochemistry; human	Knight G, Pugh A, Yates E, Bell I, Wilson R, Moody C, et al. A cyclin-binding motif in human papillomavirus type 18 (HPV18) E1^E4 is necessary for association with CDK-cyclin complexes and G2/M cell cycle arrest of keratinocytes, but is not required for differentiation-dependent viral genome amplification or L. Virology. 2011;412:196-210 pubmed publisher
immunohistochemistry; bovine western blot; bovine; 1:200	Uzbekova S, Arlot Bonnemains Y, Dupont J, Dalbies Tran R, Papillier P, Pennetier S, et al. Spatio-temporal expression patterns of aurora kinases a, B, and C and cytoplasmic polyadenylation-element-binding protein in bovine oocytes during meiotic maturation. Biol Reprod. 2008;78:218-33 pubmed
western blot; bovine; 1:1000	Tosca L, Uzbekova S, Chabrolle C, Dupont J. Possible role of 5'AMP-activated protein kinase in the metformin-mediated arrest of bovine oocytes at the germinal vesicle stage during in vitro maturation. Biol Reprod. 2007;77:452-65 pubmed
western blot; human; 1:500	Wiseman D, Werner S, Crowell P. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. J Pharmacol Exp Ther. 2007;320:1163-70 pubmed
immunocytochemistry; human; 1:100	Brito D, Rieder C. Mitotic checkpoint slippage in humans occurs via cyclin B destruction in the presence of an active checkpoint. Curr Biol. 2006;16:1194-200 pubmed
western blot; human; 1 ug/ml	Lokeshwar V, Cerwinka W, Lokeshwar B. HYAL1 hyaluronidase: a molecular determinant of bladder tumor growth and invasion. Cancer Res. 2005;65:2243-50 pubmed
western blot; human	Davy C, Jackson D, Raj K, Peh W, Southern S, Das P, et al. Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes. J Virol. 2005;79:3998-4011 pubmed publisher
	Valencia C, P xe9 rez Garc xed a F, Águila L, Felmer R, Arias M. Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence. Int J Mol Sci. 2023;24: pubmed publisher
	Pettinato A, Yoo D, VanOudenhove J, Chen Y, Cohn R, Ladha F, et al. Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep. 2021;35:109088 pubmed publisher
	x17b ury x144 A, Krajewski A, Klimaszewska Wi x15b niewska A, Grzanka A, Grzanka D. Expression of cyclin B1, D1 and K in non‑small cell lung cancer H1299 cells following treatment with sulforaphane. Oncol Rep. 2019;41:1313-1323 pubmed publisher
	Qiao R, Weissmann F, Yamaguchi M, Brown N, VanderLinden R, Imre R, et al. Mechanism of APC/CCDC20 activation by mitotic phosphorylation. Proc Natl Acad Sci U S A. 2016;113:E2570-8 pubmed publisher
	Tumurbaatar I, Cizmecioglu O, Hoffmann I, Grummt I, Voit R. Human Cdc14B promotes progression through mitosis by dephosphorylating Cdc25 and regulating Cdk1/cyclin B activity. PLoS ONE. 2011;6:e14711 pubmed publisher
	Davy C, Ayub M, Jackson D, Das P, McIntosh P, Doorbar J. HPV16 E1--E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm. Virology. 2006;349:230-44 pubmed publisher
	Sanchez V, McElroy A, Spector D. Mechanisms governing maintenance of Cdk1/cyclin B1 kinase activity in cells infected with human cytomegalovirus. J Virol. 2003;77:13214-24 pubmed
	Sheen J, Woo J, Dickson R. c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells. Br J Cancer. 2003;89:1479-85 pubmed
	Dellinger R, Karjian P, Neuteboom S. NB1011 induces Ser15 phosphorylation of p53 and activates the G2/M checkpoint. Anticancer Drugs. 2003;14:449-55 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin B1 Monoclonal Antibody (GNS1)

Catalog # :

MA5-14319

Quantity :

500 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Hamster, Human, Mouse

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Species :

Hamster, Human, Mouse

Clone :

GNS1

Isotype :

IgG1

Storage :

4 C

Description :

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

Immunogen :

Human cyclin B1 protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Aliases :

Ccn-2; CCNB; Ccnb1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb1; Cycb1-rs1; Cycb-4; Cycb-5; cyclin B; cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; I79_013589; OTTHUMP00000221981; OTTHUMP00000223023

more info or order :

Invitrogen product webpage