SMAD4 Monoclonal Antibody (SMD46 (DCS-46)) | Invitrogen MA5-14300 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SMAD4 Monoclonal Antibody (SMD46 (DCS-46))

catalog :

MA5-14300

quantity :

500 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SMD46 (DCS-46)

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 3

Reference
Choiniere J, Wu J, Wang L. Pyruvate Dehydrogenase Kinase 4 Deficiency Results in Expedited Cellular Proliferation through E2F1-Mediated Increase of Cyclins. Mol Pharmacol. 2017;91:189-196 pubmed publisher
Xiao D, Wen J, Li J, Wang K, Hu Z, Zhou J, et al. Effect of DPC4 gene on invasion and metastasis of colorectal carcinoma cells. Acta Biochim Biophys Sin (Shanghai). 2006;38:883-92 pubmed
Abe T, Furue M, Kondow A, Matsuzaki K, Asashima M. Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A. Mech Dev. 2005;122:671-80 pubmed

product information

Product Type :

Antibody

Product Name :

SMAD4 Monoclonal Antibody (SMD46 (DCS-46))

Catalog # :

MA5-14300

Quantity :

500 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Species :

Human

Clone :

SMD46 (DCS-46)

Isotype :

IgG1

Storage :

4 C

Description :

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-relatedprotein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

Immunogen :

Purified recombinant DPC4 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 ug/mL, Western Blot: 1-2 ug/mL

Aliases :

AW743858; D18Wsu70e; Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; DPC4; hSMAD4; JIP; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Madh4; mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; MYHRS; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4; SMAD4