This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MMP9 Monoclonal Antibody (IIA5)
catalog :
MA5-14228
quantity :
500 µL
price :
US 429
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IIA5
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 23
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:200; fig 1
AbdElazeem M, El Sayed M. The pattern of CD44 and matrix metalloproteinase 9 expression is a useful predictor of ulcerative colitis-associated dysplasia and neoplasia. Ann Diagn Pathol. 2015;19:369-74 pubmed publisher
  • immunocytochemistry; human; 1:300
  • western blot; human; 1:200
Dayer C, Stamenkovic I. Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-β (TGF-β) Activation and Fibroblast Differentiation. J Biol Chem. 2015;290:13763-78 pubmed publisher
  • immunohistochemistry; dogs; 1 ug/ml
Allam E, Allen M, Chu T, Ghoneima A, Jack Windsor L. In vivo effects of zoledronic acid on oral mucosal epithelial cells. Oral Dis. 2011;17:291-7 pubmed publisher
  • western blot; mouse; 1:400
Olsen C, Moreira J, Lukanidin E, Ambartsumian N. Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts. BMC Cancer. 2010;10:444 pubmed publisher
  • immunohistochemistry; rat; 1:100
Li S, Wu Y, Kang Y, Yin Y, Gao W, Liu Y, et al. Noninvasive limb ischemic preconditioning protects against myocardial I/R injury in rats. J Surg Res. 2010;164:162-8 pubmed publisher
  • western blot; rat
Duarte A, Duarte E, Correia A, Pires E, Barros M. Cardosins improve neuronal regeneration after cell disruption: a comparative expression study. Cell Biol Toxicol. 2009;25:99-108 pubmed publisher
  • immunocytochemistry; human; 1:30
Travaglino E, Benatti C, Malcovati L, Della Porta M, Galli A, Bonetti E, et al. Biological and clinical relevance of matrix metalloproteinases 2 and 9 in acute myeloid leukaemias and myelodysplastic syndromes. Eur J Haematol. 2008;80:216-26 pubmed
  • immunohistochemistry; mouse; 1:100
Hassane S, Claij N, Lantinga van Leeuwen I, Van Munsteren J, van Lent N, Hanemaaijer R, et al. Pathogenic sequence for dissecting aneurysm formation in a hypomorphic polycystic kidney disease 1 mouse model. Arterioscler Thromb Vasc Biol. 2007;27:2177-83 pubmed
  • immunohistochemistry; rat; 1:50
Oggionni T, Morbini P, Inghilleri S, Palladini G, Tozzi R, Vitulo P, et al. Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis. Eur J Histochem. 2006;50:317-25 pubmed
  • western blot; rat; 1:250
Kalyanasundaram A, Elmore J, Manazer J, Golden A, Franklin D, Galt S, et al. Simvastatin suppresses experimental aortic aneurysm expansion. J Vasc Surg. 2006;43:117-24 pubmed
  • western blot; rat; 1:200
Arbos M, Ferrando J, Quiles M, Vidal J, Lopez Cano M, Gil J, et al. Improved surgical mesh integration into the rat abdominal wall with arginine administration. Biomaterials. 2006;27:758-68 pubmed
Ron x10d evi x107 J, Jankovi x107 Milju x161 J, I x161 i x107 Den x10d i x107 T, Bo x17e i x107 V, x17d ivaljevi x107 V, x160 elemetjev S, et al. Predictive Significance of Two MMP-9 Promoter Polymorphisms and Acetylated c-Jun Transcription Factor for Papillary Thyroid Carcinoma Advancement. Diagnostics (Basel). 2022;12: pubmed publisher
Abdelkader N, Elbaset M, Moustafa P, Ibrahim S. Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling. Arch Pharm Res. 2022;45:475-493 pubmed publisher
Huang S, Sulistyowati E, Chao Y, Wu B, Dai Z, Hsu J, et al. In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis. Biomedicines. 2021;9: pubmed publisher
x160 imi x107 Biland x17e ija T, Vukojevi x107 K, x106 ori x107 A, Vukovi x107 Kekez I, Medvedec Miki x107 I, Lasi x107 Arapovi x107 L, et al. Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development. Life (Basel). 2021;11: pubmed publisher
Zhou H, Li L, Xie W, Wu L, Lin Y, He X. TAGLN and High-mobility Group AT-Hook 2 (HMGA2) Complex Regulates TGF-β-induced Colorectal Cancer Metastasis. Onco Targets Ther. 2020;13:10489-10498 pubmed publisher
Kamar S, Baky M, Omar A. The beneficial influence of rhubarb on 5-fluorouracil-induced ileal mucositis and the combined role of aquaporin-4, tumour necrosis factor-α, nuclear factor-kappa B & matrix metalloproteinase-9 in rat model: histological study. Anat Cell Biol. 2020;53:228-239 pubmed publisher
Jäkel L, Kuiperij H, Gerding L, Custers E, van den Berg E, Jolink W, et al. Disturbed balance in the expression of MMP9 and TIMP3 in cerebral amyloid angiopathy-related intracerebral haemorrhage. Acta Neuropathol Commun. 2020;8:99 pubmed publisher
Deng Z, Zhou L, Wang Y, Liao S, Huang Y, Shan Y, et al. Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions. Aging (Albany NY). 2020;12:11781-11793 pubmed publisher
Shahin N, Abdelkader N, Safar M. A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling. Sci Rep. 2018;8:4280 pubmed publisher
Sahlin L, Stjernholm Vladic Y, Roos N, Masironi B, Ekman Ordeberg G. Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming. Reprod Biol Endocrinol. 2008;6:36 pubmed publisher
Kirkegaard T, Hansen A, Bruun E, Brynskov J. Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease. Gut. 2004;53:701-9 pubmed
Surgucheva I, Sivak J, Fini M, Palazzo R, Surguchov A. Effect of gamma-synuclein overexpression on matrix metalloproteinases in retinoblastoma Y79 cells. Arch Biochem Biophys. 2003;410:167-76 pubmed
product information
Product Type :
Antibody
Product Name :
MMP9 Monoclonal Antibody (IIA5)
Catalog # :
MA5-14228
Quantity :
500 µL
Price :
US 429
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Rat
Applications :
Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1-2 µg/mL
Species :
Human, Rat
Clone :
IIA5
Isotype :
IgG1, kappa
Storage :
4° C
Description :
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
Immunogen :
Human native 92kDa MMP-9
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: Assay-dependent, Western Blot: 1-2 µg/mL
Aliases :
67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; 92-kDa type IV collagenase; AW743869; B/MMP9; Clg4b; collagenase type IVB; fj05a08; Gel B; gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; MANDP2; matrix metallo protease; matrix metallopeptidase 9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); Matrix metalloproteinase9; matrix metalloproteinase-9; MMP; Mmp9; MMP-9; mmp9 protein; MMP-9 protein; MMPs; Preproform of 92-kDa type IV collagenase (MMP-9): N-terminal; pro-MMP-9; type IV collagenase; type V collagenase; wu:fb02g06; wu:fb07b05; wu:fi98c09; wu:fj05a08; ZFMMP-9; zgc:64165
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA