This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MiTF Monoclonal Antibody (D5)
catalog :
MA5-14154
quantity :
500 µL
price :
US 450.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
D5
reactivity :
human, mouse, dogs, domestic sheep
application :
western blot, immunohistochemistry, immunocytochemistry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 39
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; loading ...; fig 2b
Zarei M, Giannikou K, Du H, Liu H, Duarte M, Johnson S, et al. MITF is a driver oncogene and potential therapeutic target in kidney angiomyolipoma tumors through transcriptional regulation of CYR61. Oncogene. 2021;40:112-126 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3a, s3b
  • western blot; mouse; 1:1000; loading ...; fig s1b
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2a
Choudhary P, Booth H, Gutteridge A, Surmacz B, Louca I, Steer J, et al. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage. Stem Cells Transl Med. 2017;6:490-501 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig s8
Fallahi Sichani M, Becker V, Izar B, Baker G, Lin J, Boswell S, et al. Adaptive resistance of melanoma cells to RAF inhibition via reversible induction of a slowly dividing de-differentiated state. Mol Syst Biol. 2017;13:905 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Miracco C, Toscano M, Butorano M, Baldino G, Tacchini D, Barone A, et al. Unusual clear cell, lymphoplasmacyte-rich, dural-based tumor with divergent differentiation: a tricky case mimicking a meningioma. Hum Pathol. 2015;46:1050-6 pubmed publisher
  • western blot; human
Nicholas C, Yang J, Peters S, Bill M, Baiocchi R, Yan F, et al. PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27(Kip1.). PLoS ONE. 2013;8:e74710 pubmed publisher
  • immunohistochemistry; human; 1:40
Pizem J, Nicholson K, Mraz J, Prieto V. Melanocytic differentiation is present in a significant proportion of nonpigmented diffuse neurofibromas: a potential diagnostic pitfall. Am J Surg Pathol. 2013;37:1182-91 pubmed publisher
  • western blot; human
Todd J, Becker T, Kefford R, Rizos H. Secondary c-Kit mutations confer acquired resistance to RTK inhibitors in c-Kit mutant melanoma cells. Pigment Cell Melanoma Res. 2013;26:518-26 pubmed publisher
  • western blot; human
Ondrusová L, Vachtenheim J, Réda J, Záková P, Benkova K. MITF-independent pro-survival role of BRG1-containing SWI/SNF complex in melanoma cells. PLoS ONE. 2013;8:e54110 pubmed publisher
  • immunohistochemistry; human; 1:40
Duval C, Chagnoleau C, Pouradier F, Sextius P, Condom E, Bernerd F. Human skin model containing melanocytes: essential role of keratinocyte growth factor for constitutive pigmentation-functional response to ?-melanocyte stimulating hormone and forskolin. Tissue Eng Part C Methods. 2012;18:947-57 pubmed publisher
  • immunohistochemistry; human; 1:40
Shirley S, Greene V, Duncan L, Torres Cabala C, Grimm E, Kusewitt D. Slug expression during melanoma progression. Am J Pathol. 2012;180:2479-89 pubmed publisher
  • immunohistochemistry; human
Gavino A, Woods M, Andea A. MART-1 is a reliable melanocytic marker in lichen planus-like keratosis: a study on 70 cases. Am J Dermatopathol. 2011;33:675-80 pubmed publisher
  • immunohistochemistry; human
Martignoni G, Bonetti F, Chilosi M, Brunelli M, Segala D, Amin M, et al. Cathepsin K expression in the spectrum of perivascular epithelioid cell (PEC) lesions of the kidney. Mod Pathol. 2012;25:100-11 pubmed publisher
  • western blot; human
Boyle G, Woods S, Bonazzi V, Stark M, Hacker E, Aoude L, et al. Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor. Pigment Cell Melanoma Res. 2011;24:525-37 pubmed publisher
  • immunohistochemistry; human; 1:25
Petersson F, Michal M, Vanecek T, Hora M, Trivunić S, Halbhuber Z, et al. Bilateral renal tumors; conventional clear cell carcinoma and contralateral t(6;11)/t(X;17)-like tumor Histomorphologic, immunohistochemical, ultrastructural and molecular genetic studies including the report of a novel mutation in the VHL gene. Ann Diagn Pathol. 2011;15:362-9 pubmed publisher
  • chromatin immunoprecipitation; human
  • EMSA; human
  • immunocytochemistry; human; 1:300
Li X, Kishore A, Dao D, Zheng W, Roman C, Word R. A novel isoform of microphthalmia-associated transcription factor inhibits IL-8 gene expression in human cervical stromal cells. Mol Endocrinol. 2010;24:1512-28 pubmed publisher
  • immunohistochemistry; human; 1:25
Petersson F, Michal M, Grossmann P, Franco M, Zamecnik M, Hes O. Low-grade sarcoma in classical seminoma - the first case reported. Int J Clin Exp Pathol. 2009;3:203-9 pubmed
  • immunohistochemistry; human
Mascarenhas J, Littlejohn E, Wolsky R, Young K, Nelson M, Salgia R, et al. PAX3 and SOX10 activate MET receptor expression in melanoma. Pigment Cell Melanoma Res. 2010;23:225-37 pubmed publisher
  • western blot; domestic sheep; 1:1000
Zhu Z, He J, Jia X, Jiang J, Bai R, Yu X, et al. MicroRNA-25 functions in regulation of pigmentation by targeting the transcription factor MITF in Alpaca (Lama pacos) skin melanocytes. Domest Anim Endocrinol. 2010;38:200-9 pubmed publisher
  • immunohistochemistry; human; 1:25
Kacerovska D, Michal M, Kutzner H, Rychnovsky J, Kazakov D. Metastatic desmoplastic malignant melanoma associated with low-grade myofibroblastic sarcoma. Am J Dermatopathol. 2009;31:490-4 pubmed publisher
  • immunohistochemistry; human
Martignoni G, Pea M, Gobbo S, Brunelli M, Bonetti F, Segala D, et al. Cathepsin-K immunoreactivity distinguishes MiTF/TFE family renal translocation carcinomas from other renal carcinomas. Mod Pathol. 2009;22:1016-22 pubmed publisher
  • immunohistochemistry; human
  • western blot; human; 2 ug/ml
Hasegawa J, Goto Y, Murata H, Takata M, Saida T, Imokawa G. Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin. Pigment Cell Melanoma Res. 2008;21:687-99 pubmed
  • immunohistochemistry; human
Chilosi M, Pea M, Martignoni G, Brunelli M, Gobbo S, Poletti V, et al. Cathepsin-k expression in pulmonary lymphangioleiomyomatosis. Mod Pathol. 2009;22:161-6 pubmed publisher
  • western blot; human; 1:1000
Boyle G, Pedley J, Martyn A, Banducci K, Strutton G, Brown D, et al. Macrophage inhibitory cytokine-1 is overexpressed in malignant melanoma and is associated with tumorigenicity. J Invest Dermatol. 2009;129:383-91 pubmed publisher
  • western blot; human
Bemis L, Chen R, Amato C, Classen E, Robinson S, Coffey D, et al. MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. Cancer Res. 2008;68:1362-8 pubmed publisher
  • immunohistochemistry; human; 1:100
Hisaoka M, Ishida T, Kuo T, Matsuyama A, Imamura T, Nishida K, et al. Clear cell sarcoma of soft tissue: a clinicopathologic, immunohistochemical, and molecular analysis of 33 cases. Am J Surg Pathol. 2008;32:452-60 pubmed publisher
  • western blot; human; 1:300
Roberts D, Newton R, Leonard J, Sturm R. Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes. J Cell Physiol. 2008;215:344-55 pubmed
  • immunohistochemistry; human
Demirag F, Unsal E, Tastepe I. Biphasic malignant mesothelioma cases with osseous differentiation and long survival: a review of the literature. Lung Cancer. 2007;57:233-6 pubmed
  • EMSA; human
Esumi N, Kachi S, Campochiaro P, Zack D. VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family. J Biol Chem. 2007;282:1838-50 pubmed
  • immunohistochemistry; human; 1:200
De Raeve L, Claes A, Ruiter D, van Muijen G, Roseeuw D, van Kempen L. Distinct phenotypic changes between the superficial and deep component of giant congenital melanocytic naevi: a rationale for curettage. Br J Dermatol. 2006;154:485-92 pubmed
  • immunocytochemistry; mouse
  • western blot; mouse
Wellbrock C, Marais R. Elevated expression of MITF counteracts B-RAF-stimulated melanocyte and melanoma cell proliferation. J Cell Biol. 2005;170:703-8 pubmed
  • immunohistochemistry; human; 1:100
Tadokoro T, Yamaguchi Y, Batzer J, Coelho S, Zmudzka B, Miller S, et al. Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation. J Invest Dermatol. 2005;124:1326-32 pubmed
  • immunohistochemistry; human
Busam K, Kucukgol D, Sato E, Frosina D, Teruya Feldstein J, Jungbluth A. Immunohistochemical analysis of novel monoclonal antibody PNL2 and comparison with other melanocyte differentiation markers. Am J Surg Pathol. 2005;29:400-6 pubmed
Hanniford D, Ulloa Morales A, Karz A, Berzoti Coelho M, Moubarak R, Sánchez Sendra B, et al. Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis. Cancer Cell. 2020;37:55-70.e15 pubmed publisher
Deyrup A, Althof P, Zhou M, Morgan M, Solomon A, Bridge J, et al. Paraganglioma-like dermal melanocytic tumor: a unique entity distinct from cellular blue nevus, clear cell sarcoma, and cutaneous melanoma. Am J Surg Pathol. 2004;28:1579-86 pubmed
Martínez Barba E, Polo García L, Ferri Niguez B, Ruiz Maciá J, Kutzner H, Requena L. Congenital giant melanocytic nevus with pigmented epithelioid cells: a variant of epithelioid blue nevus. Am J Dermatopathol. 2002;24:30-5 pubmed
Xu X, Chu A, Pasha T, Elder D, Zhang P. Immunoprofile of MITF, tyrosinase, melan-A, and MAGE-1 in HMB45-negative melanomas. Am J Surg Pathol. 2002;26:82-7 pubmed
Prasad M, Jungbluth A, Iversen K, Huvos A, Busam K. Expression of melanocytic differentiation markers in malignant melanomas of the oral and sinonasal mucosa. Am J Surg Pathol. 2001;25:782-7 pubmed
Miettinen M, Fernandez M, Franssila K, Gatalica Z, Lasota J, Sarlomo Rikala M. Microphthalmia transcription factor in the immunohistochemical diagnosis of metastatic melanoma: comparison with four other melanoma markers. Am J Surg Pathol. 2001;25:205-11 pubmed
product information
Product Type :
Antibody
Product Name :
MiTF Monoclonal Antibody (D5)
Catalog # :
MA5-14154
Quantity :
500 µL
Price :
US 450.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Canine, Human
Applications :
Gel Shift: 1 mg/mL, Immunohistochemistry (Paraffin): 4-8 µg/mL
Species :
Canine, Human
Clone :
D5
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
Immunogen :
N-terminal fragment of human Mi protein
Format :
Liquid
Applications w/Dilutions :
Gel Shift: 1 mg/mL, Immunohistochemistry (Paraffin): 4-8 µg/mL
Aliases :
BCC2; Bhlhe32; black eyed white; bw; Class E basic helix-loop-helix protein 32; CMM8; Gsfbcc2; melanocyte inducing transcription factor; melanogenesis associated transcription factor; mi; microphtalmia-associated transcription factor; microphthalmia; microphthalmia associated transcription factor isoform M-; microphthalmia transcription factor; microphthalmia-associated transcription factor; microphthalmia-associated transcription factor A; microphthalmia-associated transcription factor isoform H; microphthalmia-associated transcription factor isoform H protein; microphthalmia-associated transcription factor Trnc-1; microphthalmia-associated transcription factor Trnc-2; microphthalmia-associated transcription factor variant 1; microphthalmia-associated transcription factor variant 2; microphthalmia-associated transcription factor variant 3; microphthalmia-associated transcription factor variant MITF-M(+); MITF; MITF-H; MITF-M; MITF-M-; transcription factor, isoform 7; vit; Vitiligo; Wh; WS2; WS2A
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA