Bax Monoclonal Antibody (2D2), Biotin | Invitrogen MA5-13991 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Bax Monoclonal Antibody (2D2), Biotin

catalog :

MA5-13991

quantity :

500 uL

price :

US 524.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

2D2

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 44

Published Application/Species/Sample/Dilution	Reference
western blot; human	Essabbani A, Garcia L, Zonetti M, Fisco T, Pucci S, Chiocchia G. Exon-skipping strategy by ratio modulation between cytoprotective versus pro-apoptotic clusterin forms increased sensitivity of LNCaP to cell death. PLoS ONE. 2013;8:e54920 pubmed publisher
immunohistochemistry; human; 1:400	Mitra A, Castelao J, Hawes D, Tsao Wei D, Jiang X, Shi S, et al. Combination of molecular alterations and smoking intensity predicts bladder cancer outcome: a report from the Los Angeles Cancer Surveillance Program. Cancer. 2013;119:756-65 pubmed publisher
western blot; human	Slipicevic A, Øy G, Rosnes A, Stakkestad Ø, Emilsen E, Engesæter B, et al. Low-dose anisomycin sensitizes melanoma cells to TRAIL induced apoptosis. Cancer Biol Ther. 2013;14:146-54 pubmed publisher
immunohistochemistry; human	Ozer H, Yenicesu G, Arici S, Cetin M, Tuncer E, Cetin A. Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors. Diagn Pathol. 2012;7:124 pubmed publisher
immunohistochemistry; rat; 1:100	Baykara B, Micili S, Tugyan K, Tekmen I, Bagriyanik H, Sonmez U, et al. The protective effects of carnosine in alcohol-induced hepatic injury in rats. Toxicol Ind Health. 2014;30:25-32 pubmed publisher
immunohistochemistry; human	Lazarevic B, Hammarstrom C, Yang J, Ramberg H, Diep L, Karlsen S, et al. The effects of short-term genistein intervention on prostate biomarker expression in patients with localised prostate cancer before radical prostatectomy. Br J Nutr. 2012;108:2138-47 pubmed publisher
immunohistochemistry; human	Gao C, Guo W, Wang A. Significance of decreased apoptosis, role of Bid expression and Bcl-Xl/Bid ratio in human jejunal stromal tumors. APMIS. 2012;120:171-81 pubmed publisher
immunohistochemistry; human; 1:300	Arvai K, Nagy K, Barti Juhasz H, Petak I, Krenacs T, Micsik T, et al. Molecular profiling of parathyroid hyperplasia, adenoma and carcinoma. Pathol Oncol Res. 2012;18:607-14 pubmed publisher
immunohistochemistry; human	Aydin F, Levent Y, Nilgun S, Pancar Y, Yasar T. Implications of bax, fas, and p53 in the pathogenesis of early-stage mycosis fungoides and alterations in expression following photochemotherapy. Indian J Dermatol. 2011;56:501-4 pubmed publisher
western blot; human	Tian L, Yue W, Zhu F, Li S, Li W. Human mesenchymal stem cells play a dual role on tumor cell growth in vitro and in vivo. J Cell Physiol. 2011;226:1860-7 pubmed publisher
western blot; human; 1:100	Yu F, Hao X, Zhao H, Ge C, Yao M, Yang S, et al. Delta-like 1 contributes to cell growth by increasing the interferon-inducible protein 16 expression in hepatocellular carcinoma. Liver Int. 2010;30:703-14 pubmed publisher
western blot; human	Li C, Cheng Y, Liao P, Kang J. Translocation of p53 to mitochondria is regulated by its lipid binding property to anionic phospholipids and it participates in cell death control. Neoplasia. 2010;12:150-60 pubmed
western blot; human	Wu J, Tsai K, Shee J, Li Y, Chen C, Chuang J, et al. 4'-Chloro-3,5-dihydroxystilbene, a resveratrol derivative, induces lung cancer cell death. Acta Pharmacol Sin. 2010;31:81-92 pubmed publisher
immunohistochemistry; human; 1:100	Karamitopoulou E, Rentsch C, Markwalder R, Vallan C, Thalmann G, Brunner T. Prognostic significance of apoptotic cell death in bladder cancer: a tissue microarray study on 179 urothelial carcinomas from cystectomy specimens. Pathology. 2010;42:37-42 pubmed publisher
immunohistochemistry; human	Gao C, Wang A. Significance of increased apoptosis and Bax expression in human small intestinal adenocarcinoma. J Histochem Cytochem. 2009;57:1139-48 pubmed publisher
immunohistochemistry; human; 1:50	Song K, Jung C, Park W, Park C. Expression of the antiapoptosis gene Survivin predicts poor prognosis of stage III gastric adenocarcinoma. Jpn J Clin Oncol. 2009;39:290-6 pubmed publisher
immunoprecipitation; human immunohistochemistry; human western blot; human	Pucci S, Mazzarelli P, Paola M, Sesti F, Fabiola S, Boothman D, et al. Interleukin-6 affects cell death escaping mechanisms acting on Bax-Ku70-Clusterin interactions in human colon cancer progression. Cell Cycle. 2009;8:473-81 pubmed
western blot; human	Lin Y, Hou Y, Lin C, Hsu Y, Sheu J, Lai C, et al. Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells. Biochem Biophys Res Commun. 2009;378:683-8 pubmed publisher
immunohistochemistry; human; 1:100	Gol M, Tuna B. Effect of fetal sex on apoptosis-regulating proteins in trophoblasts of full-term human placenta. Gynecol Obstet Invest. 2009;67:53-6 pubmed publisher
immunohistochemistry; human	Gao C, Wang A, Han Y. Microwave antigen retrieval blocks endogenous peroxidase activity in immunohistochemistry. Appl Immunohistochem Mol Morphol. 2008;16:393-9 pubmed publisher
immunohistochemistry; human	Murphy K, Chen F, Clark D. Identification of immunohistochemical biomarkers for papillary thyroid carcinoma using gene expression profiling. Hum Pathol. 2008;39:420-6 pubmed publisher
immunohistochemistry; human; 200 ug/ml	Aktas S, Kargi A, Olgun N, Diniz G, Erbay A, Vergin C. Prognostic significance of cell proliferation and apoptosis-regulating proteins in Epstein-Barr virus positive and negative pediatric Hodgkin lymphoma. Lymphat Res Biol. 2007;5:175-82 pubmed
immunocytochemistry; human	Ricci F, Pucci S, Sesti F, Missiroli F, Cerulli L, Spagnoli L. Modulation of Ku70/80, clusterin/ApoJ isoforms and Bax expression in indocyanine-green-mediated photo-oxidative cell damage. Ophthalmic Res. 2007;39:164-73 pubmed
western blot; human	Mukherjee Nee Chakraborty S, Ghosh U, Bhattacharyya N, Bhattacharya R, Dey S, Roy M. Curcumin-induced apoptosis in human leukemia cell HL-60 is associated with inhibition of telomerase activity. Mol Cell Biochem. 2007;297:31-9 pubmed
western blot; human; 1:2000	Chakraborty S, Kundu T, Dey S, Bhattacharya R, Siddiqi M, Roy M. Tea-induced apoptosis in human leukemia K562 cells as assessed by comet formation. Asian Pac J Cancer Prev. 2006;7:201-7 pubmed
western blot; human	Yang Y, Hsu Y, Wu C, Chen H, Chang M. Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells. Biochem Biophys Res Commun. 2006;343:428-34 pubmed
western blot; human	Nishikawa T, Nakajima T, Moriguchi M, Jo M, Sekoguchi S, Ishii M, et al. A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. J Hepatol. 2006;44:1074-82 pubmed
western blot; human; 1:2000	Chakraborty S, Ghosh U, Bhattacharyya N, Bhattacharya R, Roy M. Inhibition of telomerase activity and induction of apoptosis by curcumin in K-562 cells. Mutat Res. 2006;596:81-90 pubmed
western blot; human; 1:2000	Kundu T, Dey S, Roy M, Siddiqi M, Bhattacharya R. Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin. Cancer Lett. 2005;230:111-21 pubmed
immunohistochemistry; human; 1:150	Torlakovic E, Nielsen S, Vyberg M. Antibody selection in immunohistochemical detection of cyclin D1 in mantle cell lymphoma. Am J Clin Pathol. 2005;124:782-9 pubmed
western blot; human	Skvortsov S, Skvortsova I, Sarg B, Loeffler Ragg J, Lindner H, Lukas P, et al. Irreversible pan-ErbB tyrosine kinase inhibitor CI-1033 induces caspase-independent apoptosis in colorectal cancer DiFi cell line. Apoptosis. 2005;10:1175-86 pubmed
immunohistochemistry; human; 1:100	Bassarova A, Nesland J, Sedloev T, Lilleby W, Hristova S, Trifonov D, et al. Simultaneous bilateral breast carcinomas: a category with frequent coexpression of HER-2 and ER-alpha, high Ki-67 and bcl-2, and low p53. Int J Surg Pathol. 2005;13:239-46 pubmed
immunohistochemistry; human	Kokturk N, Firat P, Akay H, Kadilar C, Ozturk C, Zorlu F, et al. Prognostic significance of Bax and Fas ligand in erionite and asbestos induced Turkish malignant pleural mesothelioma. Lung Cancer. 2005;50:189-98 pubmed
immunohistochemistry; human; 1:100	Narula N, Narula J, Zhang P, Haider N, Raghunath P, Brittin R, et al. Is the myofibrillarlytic myocyte a forme fruste apoptotic myocyte?. Ann Thorac Surg. 2005;79:1333-7; discussion 1337 pubmed
	Alkhedaide A, Alshehri Z, Sabry A, Abdel Ghaffar T, Soliman M, Attia H. Protective effect of grape seed extract against cadmium-induced testicular dysfunction. Mol Med Rep. 2016;13:3101-9 pubmed publisher
	Sirvent J, Aguilar M, Olona M, Pelegri A, Blazquez S, Gutierrez C. Prognostic value of apoptosis in breast cancer (pT1-pT2). A TUNEL, p53, bcl-2, bag-1 and Bax immunohistochemical study. Histol Histopathol. 2004;19:759-70 pubmed publisher
	Liu S, Leung R, Chan K, Chiu P, Cheung A, Tam K, et al. p73 expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy. Clin Cancer Res. 2004;10:3309-16 pubmed
	Bassarova A, Nesland J, Sedloev T, Danielsen H, Christova S. Pilomatrix carcinoma with lymph node metastases. J Cutan Pathol. 2004;31:330-5 pubmed
	Qiu L, Sinniah R, I Hong Hsu S. Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy. J Am Soc Nephrol. 2004;15:79-90 pubmed
	Gourdier I, Del Rio M, Crabbe L, Candeil L, Copois V, Ychou M, et al. Drug specific resistance to oxaliplatin is associated with apoptosis defect in a cellular model of colon carcinoma. FEBS Lett. 2002;529:232-6 pubmed
	Ortiz Rey J, Alvarez Alvarez C, Antón Badiola I, San Miguel Fraile P, de la Fuente Buceta A. Human Meissner corpuscles express Bcl-2 but not Bax protein. Neurosci Lett. 2002;329:240-2 pubmed
	Bukholm I, Bukholm G, Nesland J. Reduced expression of both Bax and Bcl-2 is independently associated with lymph node metastasis in human breast carcinomas. APMIS. 2002;110:214-20 pubmed
	Lopes J, Nesland J, Reis Filho J, Holm R. Differential Ki67 and bcl-2 immunoexpression in solid-glandular and spindle cell components of biphasic synovial sarcoma: a double immunostaining assessment with cytokeratin and vimentin. Histopathology. 2002;40:464-71 pubmed
	Ong F, Moonen L, Gallee M, ten Bosch C, Zerp S, Hart A, et al. Prognostic factors in transitional cell cancer of the bladder: an emerging role for Bcl-2 and p53. Radiother Oncol. 2001;61:169-75 pubmed

product information

Product Type :

Antibody

Product Name :

Bax Monoclonal Antibody (2D2), Biotin

Catalog # :

MA5-13991

Quantity :

500 uL

Price :

US 524.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 2-4 ug/mL, Western Blot: 1 ug/mL

Species :

Human

Clone :

2D2

Isotype :

IgG1, kappa

Storage :

4 C

Description :

BAX is a members of the Bcl-2 Family and plays an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of BAX and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa). BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.

Immunogen :

A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein. This amino acid sequence is NOT shared by mouse and rat bax protein.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 2-4 ug/mL, Western Blot: 1 ug/mL

Aliases :

apoptosis regulator BAX; Apoptosis regulator Bcl-X; Bax; Bax zeta; BAX-ALPHA; Bax-alpha protein; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2 associated X, apoptosis regulator; BCL2 like 1; BCL2-associated protein; Bcl-2-associated protein Bax; Bcl2-associated protein Bax; Bcl-2-associated X protein; BCL2-associated X protein; BCL2-associated X protein omega; BCL2L; BCL2L1; Bcl2-L-1; BCL2L4; Bcl2-L-4; BCL2-like 1; bcl-2-like protein 1; Bcl-2-like protein 4; BCLX; Bcl-X; BCL-XL/S; PPP1R52; protein phosphatase 1, regulatory subunit 52

more info or order :

Invitrogen product webpage