This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
EGFR Monoclonal Antibody (H9B4)
catalog :
MA5-13877
quantity :
500 µL
price :
US 486.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H9B4
reactivity :
human, mouse, fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 26
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:1000; loading ...; fig 4b
  • western blot; human; 1:1000; loading ...; fig 4a
Wu Y, Wang Y, Lin Y, Liu Y, Wang Y, Jia J, et al. Dub3 inhibition suppresses breast cancer invasion and metastasis by promoting Snail1 degradation. Nat Commun. 2017;8:14228 pubmed publisher
  • western blot; human; loading ...; fig 1b
Day K, Lorenzatti Hiles G, Kozminsky M, Dawsey S, Paul A, Broses L, et al. HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone. Cancer Res. 2017;77:74-85 pubmed publisher
  • immunohistochemistry; human; 1:200; fig 6a
  • western blot; human; loading ...; fig 1b
Engel B, Bowser J, Broaddus R, Carson D. MUC1 stimulates EGFR expression and function in endometrial cancer. Oncotarget. 2016;7:32796-809 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Arasada R, Amann J, Rahman M, Huppert S, Carbone D. EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling. Cancer Res. 2014;74:5572-84 pubmed publisher
  • western blot; human; fig 1
Grivas P, Day K, Karatsinides A, Paul A, Shakir N, Owainati I, et al. Evaluation of the antitumor activity of dacomitinib in models of human bladder cancer. Mol Med. 2013;19:367-76 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Odintsova E, van Niel G, Conjeaud H, Raposo G, Iwamoto R, Mekada E, et al. Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor. J Biol Chem. 2013;288:26323-34 pubmed publisher
  • immunoprecipitation; mouse; 1:1000
Brouxhon S, Kyrkanides S, Teng X, O Banion M, Clarke R, Byers S, et al. Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers. Mol Carcinog. 2014;53:893-906 pubmed publisher
  • western blot; human; 1:1000; fig 3
Neeraja Dharmaraj -, Engel B, Carson D. Activated EGFR stimulates MUC1 expression in human uterine and pancreatic cancer cell lines. J Cell Biochem. 2013;114:2314-22 pubmed publisher
  • western blot; human; 1:5000; fig 1
Danysh B, Constantinou P, Lukianova Hleb E, Lapotko D, Carson D. The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation. Theranostics. 2012;2:777-87 pubmed publisher
  • western blot; human
Fan P, McDaniel R, Kim H, Clagett D, Haddad B, Jordan V. Modulating therapeutic effects of the c-Src inhibitor via oestrogen receptor and human epidermal growth factor receptor 2 in breast cancer cell lines. Eur J Cancer. 2012;48:3488-98 pubmed publisher
  • immunoprecipitation; human; 1 ug/time
  • western blot; human
Grabowska M, Sandhu B, Day M. EGF promotes the shedding of soluble E-cadherin in an ADAM10-dependent manner in prostate epithelial cells. Cell Signal. 2012;24:532-8 pubmed publisher
  • immunoprecipitation; human; 2-5 ug/time
DeFazio Eli L, Strommen K, Dao Pick T, Parry G, Goodman L, Winslow J. Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action. Breast Cancer Res. 2011;13:R44 pubmed publisher
  • immunocytochemistry; human
Shi Y, Huang W, Tan Y, Jin X, Dua R, Penuel E, et al. A novel proximity assay for the detection of proteins and protein complexes: quantitation of HER1 and HER2 total protein expression and homodimerization in formalin-fixed, paraffin-embedded cell lines and breast cancer tissue. Diagn Mol Pathol. 2009;18:11-21 pubmed publisher
  • western blot; human
Deb T, Coticchia C, Barndt R, Zuo H, Dickson R, Johnson M. Pregnancy-upregulated nonubiquitous calmodulin kinase induces ligand-independent EGFR degradation. Am J Physiol Cell Physiol. 2008;295:C365-77 pubmed publisher
  • immunocytochemistry; mouse; 1:50; loading ...; fig 5
  • western blot; mouse; loading ...; fig 3a
Choi S, Mendrola J, Lemmon M. EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer. Oncogene. 2007;26:1567-76 pubmed
  • western blot; human; 1:1000
Blehm K, Spiess P, Bondaruk J, Dujka M, Villares G, Zhao Y, et al. Mutations within the kinase domain and truncations of the epidermal growth factor receptor are rare events in bladder cancer: implications for therapy. Clin Cancer Res. 2006;12:4671-7 pubmed
  • western blot; human
Odintsova E, Butters T, Monti E, Sprong H, van Meer G, Berditchevski F. Gangliosides play an important role in the organization of CD82-enriched microdomains. Biochem J. 2006;400:315-25 pubmed
  • western blot; human
Contessa J, Abell A, Valerie K, Lin P, Schmidt Ullrich R. ErbB receptor tyrosine kinase network inhibition radiosensitizes carcinoma cells. Int J Radiat Oncol Biol Phys. 2006;65:851-8 pubmed
  • western blot; human; 1:1000; fig 6
Kraemer K, Schmidt U, Fuessel S, Herr A, Wirth M, Meye A. Microarray analyses in bladder cancer cells: inhibition of hTERT expression down-regulates EGFR. Int J Cancer. 2006;119:1276-84 pubmed
  • immunoprecipitation; human
Contessa J, Abell A, Mikkelsen R, Valerie K, Schmidt Ullrich R. Compensatory ErbB3/c-Src signaling enhances carcinoma cell survival to ionizing radiation. Breast Cancer Res Treat. 2006;95:17-27 pubmed
  • western blot; fruit fly ; fig 4b
Dawson J, Berger M, Lin C, Schlessinger J, Lemmon M, Ferguson K. Epidermal growth factor receptor dimerization and activation require ligand-induced conformational changes in the dimer interface. Mol Cell Biol. 2005;25:7734-42 pubmed
  • immunoprecipitation; human
Sturla L, Amorino G, Alexander M, Mikkelsen R, Valerie K, Schmidt Ullrichr R. Requirement of Tyr-992 and Tyr-1173 in phosphorylation of the epidermal growth factor receptor by ionizing radiation and modulation by SHP2. J Biol Chem. 2005;280:14597-604 pubmed
  • western blot; human
Kansra S, Stoll S, Johnson J, Elder J. Src family kinase inhibitors block amphiregulin-mediated autocrine ErbB signaling in normal human keratinocytes. Mol Pharmacol. 2005;67:1145-57 pubmed
Hern xe1 ndez Bule M, Mart xed nez M, Trillo M, Mart xed nez L, Toledano Mac xed as E, xda beda A. Response of human cancer cells to simultaneous treatment with sorafenib and radiofrequency current. Oncol Lett. 2021;22:807 pubmed publisher
Tamura S, Wang Y, Veeneman B, Hovelson D, Bankhead A, Broses L, et al. Molecular Correlates of In Vitro Responses to Dacomitinib and Afatinib in Bladder Cancer. Bladder Cancer. 2018;4:77-90 pubmed publisher
Genther S, Sterling S, Duensing S, Munger K, Sattler C, Lambert P. Quantitative role of the human papillomavirus type 16 E5 gene during the productive stage of the viral life cycle. J Virol. 2003;77:2832-42 pubmed
product information
Product Type :
Antibody
Product Name :
EGFR Monoclonal Antibody (H9B4)
Catalog # :
MA5-13877
Quantity :
500 µL
Price :
US 486.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
Species :
Human
Clone :
H9B4
Isotype :
IgG1
Storage :
4° C
Description :
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Immunogen :
Purified EGFR from A431 cells
Format :
Liquid
Applications w/Dilutions :
Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
Aliases :
2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA