This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Ezrin Monoclonal Antibody (3C12)
catalog :
MA5-13862
quantity :
500 µL
price :
US 496.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3C12
reactivity :
Chinese hamsters, human, mouse, rat, dogs, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 75
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:500; loading ...; fig s3a
Marques P, Nyegaard S, Collins R, Troise F, Freeman S, Trimble W, et al. Multimerization and Retention of the Scavenger Receptor SR-B1 in the Plasma Membrane. Dev Cell. 2019;: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 2a
Fathi A, Mosaad H, Hussein S, Roshdy M, Ismail E. Prognostic significance of CD133 and ezrin expression in colorectal carcinoma. IUBMB Life. 2017;69:328-340 pubmed publisher
  • western blot; human; loading ...; fig 1b
Riecken L, Zoch A, Wiehl U, Reichert S, Scholl I, Cui Y, et al. CPI-17 drives oncogenic Ras signaling in human melanomas via Ezrin-Radixin-Moesin family proteins. Oncotarget. 2016;7:78242-78254 pubmed publisher
  • immunohistochemistry; rat; 1:400; loading ...; fig 7d
Hayashi M, Inagaki A, Novak I, Matsuda H. The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells. Pflugers Arch. 2016;468:1171-81 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
Poncy A, Antoniou A, Cordi S, Pierreux C, Jacquemin P, Lemaigre F. Transcription factors SOX4 and SOX9 cooperatively control development of bile ducts. Dev Biol. 2015;404:136-48 pubmed publisher
  • immunohistochemistry; human; 1:1000
Costa Y, Tjioe K, Nonogaki S, Soares F, Lauris J, Oliveira D. Are podoplanin and ezrin involved in the invasion process of the ameloblastomas?. Eur J Histochem. 2015;59:2451 pubmed publisher
  • western blot; mouse
Hartmann M, Parra L, Ruschel A, Böhme S, Li Y, Morrison H, et al. Tumor Suppressor NF2 Blocks Cellular Migration by Inhibiting Ectodomain Cleavage of CD44. Mol Cancer Res. 2015;13:879-90 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
Cao H, Zhang S, SHEN J, Wu Z, Wu J, Wang S, et al. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6:5435-48 pubmed
  • immunohistochemistry; human; 1:100
Gao W, Zhang C, Feng Y, Chen G, Wen S, Huangfu H, et al. Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma. PLoS ONE. 2012;7:e50710 pubmed publisher
  • immunocytochemistry; dogs
  • western blot; dogs
Chen J, Lin Y, Jou T. Phosphorylation of EBP50 negatively regulates ?-PIX-dependent Rac1 activity in anoikis. Cell Death Differ. 2012;19:1027-37 pubmed publisher
  • immunocytochemistry; mouse; 1:50
Prevot P, Simion A, Grimont A, Colletti M, Khalaileh A, Van den Steen G, et al. Role of the ductal transcription factors HNF6 and Sox9 in pancreatic acinar-to-ductal metaplasia. Gut. 2012;61:1723-32 pubmed publisher
  • immunohistochemistry; human; 1:100
Schlecht N, Brandwein Gensler M, Smith R, Kawachi N, Broughel D, Lin J, et al. Cytoplasmic ezrin and moesin correlate with poor survival in head and neck squamous cell carcinoma. Head Neck Pathol. 2012;6:232-43 pubmed publisher
  • western blot; human
Sperka T, Geissler K, Merkel U, Scholl I, Rubio I, Herrlich P, et al. Activation of Ras requires the ERM-dependent link of actin to the plasma membrane. PLoS ONE. 2011;6:e27511 pubmed publisher
  • immunohistochemistry; human; 1:200
Pasonen Seppänen S, Hyttinen J, Rilla K, Jokela T, Noble P, Tammi M, et al. Role of CD44 in the organization of keratinocyte pericellular hyaluronan. Histochem Cell Biol. 2012;137:107-20 pubmed publisher
  • immunohistochemistry; human; 1:300
Athanasopoulou A, Aroukatos P, Nakas D, Repanti M, Papadaki H, Bravou V. Decreased ezrin and paxillin expression in human urothelial bladder tumors correlate with tumor progression. Urol Oncol. 2013;31:836-42 pubmed publisher
  • immunohistochemistry; human; 1:400
  • western blot; human
Xie J, Zhang F, Tao L, Lu Z, Xu X, Jian Shen -, et al. Expression of ezrin in human embryonic, fetal, and normal adult tissues. J Histochem Cytochem. 2011;59:1001-8 pubmed publisher
  • immunohistochemistry - paraffin section; human
Almeida G, Castro G, Snitcovsky I, Siqueira S, Akaishi E, Camargo O, et al. Sequential dose-dense Doxorubicin and Ifosfamide in advanced soft-tissue sarcoma patients in an out-patient-basis schedule. Sarcoma. 2011;2011:984340 pubmed publisher
  • western blot; human; fig 1
Olaku V, Matzke A, Mitchell C, Hasenauer S, Sakkaravarthi A, Pace G, et al. c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice. Mol Biol Cell. 2011;22:2777-86 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
  • western blot; human; 1:2000; fig 5
Riquelme G, Vallejos C, de Gregorio N, Morales B, Godoy V, Berrios M, et al. Lipid rafts and cytoskeletal proteins in placental microvilli membranes from preeclamptic and IUGR pregnancies. J Membr Biol. 2011;241:127-40 pubmed publisher
  • western blot; human
Zhang Y, Dong J, Zhu X, Wang W, Yang Q. The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain. Biochem Pharmacol. 2011;82:287-94 pubmed publisher
  • immunohistochemistry; human
Wang Y, Chen W, Huang Z, Yang Z, Zhang B, Wang J, et al. Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112:96-104 pubmed publisher
  • western blot; human; 1:100
Ou Yang M, Liu H, Zhang Y, Zhu X, Yang Q. ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells. Biochimie. 2011;93:954-61 pubmed publisher
  • western blot; human; fig 2
Natoli M, Leoni B, D Agnano I, D Onofrio M, Brandi R, Arisi I, et al. Cell growing density affects the structural and functional properties of Caco-2 differentiated monolayer. J Cell Physiol. 2011;226:1531-43 pubmed publisher
  • western blot; mouse
Shaffer M, Huang Y, Corbo E, Wu G, Velez M, Choi J, et al. Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice. PLoS ONE. 2010;5:e12404 pubmed publisher
  • immunohistochemistry; human; fig 7
Hara M, Yanagihara T, Hirayama Y, Ogasawara S, Kurosawa H, Sekine S, et al. Podocyte membrane vesicles in urine originate from tip vesiculation of podocyte microvilli. Hum Pathol. 2010;41:1265-75 pubmed publisher
  • immunohistochemistry - paraffin section; human; tbl 1
Giusiano S, Secq V, Carcopino X, Carpentier S, Andrac L, Lavaut M, et al. Immunohistochemical profiling of node negative breast carcinomas allows prediction of metastatic risk. Int J Oncol. 2010;36:889-98 pubmed
  • immunohistochemistry; human
Zhai J, Yang X, Yang F, Hu J, Hua W. Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma. Chin J Cancer. 2010;29:317-20 pubmed
  • western blot; human
Mao D, Hinds P. p35 is required for CDK5 activation in cellular senescence. J Biol Chem. 2010;285:14671-80 pubmed publisher
  • western blot; human
Chuan Y, Iglesias Gato D, Fernandez Perez L, Cedazo Minguez A, Pang S, Norstedt G, et al. Ezrin mediates c-Myc actions in prostate cancer cell invasion. Oncogene. 2010;29:1531-42 pubmed publisher
  • immunohistochemistry; human; 1:1500
  • western blot; human
Yeh C, Pang S, Chen T, Wu R, Weng W, Chen M. Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinoma. BMC Cancer. 2009;9:233 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Morris Z, McClatchey A. Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma. Proc Natl Acad Sci U S A. 2009;106:9767-72 pubmed publisher
  • immunoprecipitation; rat
Fukasawa H, Bornheimer S, Kudlicka K, Farquhar M. Slit diaphragms contain tight junction proteins. J Am Soc Nephrol. 2009;20:1491-503 pubmed publisher
  • western blot; mouse
Jung C, Matzke A, Niemann H, Schwerk C, Tenenbaum T, Orian Rousseau V. Involvement of CD44v6 in InlB-dependent Listeria invasion. Mol Microbiol. 2009;72:1196-207 pubmed publisher
  • immunohistochemistry; human; tbl 3
Charpin C, Giusiano S, Secq V, Carpentier S, Andrac L, Lavaut M, et al. Quantitative immunocytochemical profile to predict early outcome of disease in triple-negative breast carcinomas. Int J Oncol. 2009;34:983-93 pubmed
  • immunohistochemistry; human; 1:40
Fouassier L, Rosenberg P, Mergey M, Saubamea B, Clapéron A, Kinnman N, et al. Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation. Am J Pathol. 2009;174:869-80 pubmed publisher
  • immunohistochemistry - paraffin section; human; tbl 1
  • immunohistochemistry; human
Charpin C, Secq V, Giusiano S, Carpentier S, Andrac L, Lavaut M, et al. A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays. Int J Cancer. 2009;124:2124-34 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Morand S, Ueyama T, Tsujibe S, Saito N, Korzeniowska A, Leto T. Duox maturation factors form cell surface complexes with Duox affecting the specificity of reactive oxygen species generation. FASEB J. 2009;23:1205-18 pubmed publisher
  • immunocytochemistry; human
  • western blot; human; 1:400
Chifenti B, Morelli M, Zavaglia M, Coviello D, Guerneri S, Santucci A, et al. Establishment and characterization of 4 new human pancreatic cancer cell lines: evidences of different tumor phenotypes. Pancreas. 2009;38:184-96 pubmed publisher
  • western blot; human; fig 2
Bovenschen N, de Koning P, Quadir R, Broekhuizen R, Damen J, Froelich C, et al. NK cell protease granzyme M targets alpha-tubulin and disorganizes the microtubule network. J Immunol. 2008;180:8184-91 pubmed
  • immunohistochemistry; rat
  • immunohistochemistry; human
Daniel C, Albrecht H, Lüdke A, Hugo C. Nestin expression in repopulating mesangial cells promotes their proliferation. Lab Invest. 2008;88:387-97 pubmed publisher
  • immunohistochemistry; human; 1:200
  • western blot; human
Li Q, Wu M, Wang H, Xu G, Zhu T, Zhang Y, et al. Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells. Cancer Lett. 2008;261:55-63 pubmed
  • immunoprecipitation; human
  • western blot; human
Ruppelt A, Mosenden R, Grönholm M, Aandahl E, Tobin D, Carlson C, et al. Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin. J Immunol. 2007;179:5159-68 pubmed
  • immunohistochemistry; human
Torer N, Kayaselcuk F, Nursal T, Yildirim S, Tarim A, Noyan T, et al. Adhesion molecules as prognostic markers in pancreatic adenocarcinoma. J Surg Oncol. 2007;96:419-23 pubmed
  • immunocytochemistry; human
  • western blot; human
Yang Q, Onuki R, Nakai C, Sugiyama Y. Ezrin and radixin both regulate the apical membrane localization of ABCC2 (MRP2) in human intestinal epithelial Caco-2 cells. Exp Cell Res. 2007;313:3517-25 pubmed
  • immunohistochemistry; human
Bal N, Yildirim S, Nursal T, Bolat F, Kayaselcuk F. Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type. World J Gastroenterol. 2007;13:3726-9 pubmed
  • western blot; dogs
Yu C, Chen J, Lin Y, Shen K, Lin W, Chien C, et al. A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135. Mol Biol Cell. 2007;18:1710-22 pubmed
  • western blot; human
Chuan Y, Pang S, Cedazo Minguez A, Norstedt G, Pousette A, Flores Morales A. Androgen induction of prostate cancer cell invasion is mediated by ezrin. J Biol Chem. 2006;281:29938-48 pubmed
  • immunohistochemistry; human; 1:100
Madan R, Brandwein Gensler M, Schlecht N, Elias K, Gorbovitsky E, Belbin T, et al. Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma. Head Neck. 2006;28:1018-27 pubmed
  • immunohistochemistry; human; 1:200
Park H, Jung W, Bacchini P, Bertoni F, Kim Y, Park Y. Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma. Pathol Res Pract. 2006;202:509-15 pubmed
  • immunohistochemistry; human; 1:300
Valdman A, Fang X, Pang S, Nilsson B, Ekman P, Egevad L. Ezrin expression in prostate cancer and benign prostatic tissue. Eur Urol. 2005;48:852-7 pubmed
  • immunohistochemistry; human; 1:200
Weng W, Ahlén J, Astrom K, Lui W, Larsson C. Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas. Clin Cancer Res. 2005;11:6198-204 pubmed
  • immunohistochemistry; human; 1:100
Ishizawa K, Komori T, Hirose T. Stromal cells in hemangioblastoma: neuroectodermal differentiation and morphological similarities to ependymoma. Pathol Int. 2005;55:377-85 pubmed
  • immunohistochemistry; rat; 1:50
Goldwich A, Baulmann D, Ohlmann A, Flügel Koch C, Schocklmann H, Tamm E. Myocilin is expressed in the glomerulus of the kidney and induced in mesangioproliferative glomerulonephritis. Kidney Int. 2005;67:140-51 pubmed
Chen L, Perera N, Karoukis A, Feathers K, Ali R, Thompson D, et al. Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells. Sci Rep. 2022;12:12694 pubmed publisher
Hargest V, Bub T, Neale G, Schultz Cherry S. Astrovirus-induced epithelial-mesenchymal transition via activated TGF-β increases viral replication. PLoS Pathog. 2022;18:e1009716 pubmed publisher
Basu A, Penumutchu S, Nguyen K, Mbonye U, Tolbert B, Karn J, et al. A Structurally Conserved RNA Element within SARS-CoV-2 ORF1a RNA and S mRNA Regulates Translation in Response to Viral S Protein-Induced Signaling in Human Lung Cells. J Virol. 2022;96:e0167821 pubmed publisher
Wolk A, Hatipoglu D, Cutler A, Ali M, Bell L, Hua Qi J, et al. Role of FGF and Hyaluronan in Choroidal Neovascularization in Sorsby Fundus Dystrophy. Cells. 2020;9: pubmed publisher
Sundaram S, Palaniappan B, Nepal N, Chaffins S, Sundaram U, Arthur S. Mechanism of Dyslipidemia in Obesity-Unique Regulation of Ileal Villus Cell Brush Border Membrane Sodium-Bile Acid Cotransport. Cells. 2019;8: pubmed publisher
Jeong J, Choi J, Kim W, Dann P, Takyar F, Gefter J, et al. Inhibition of ezrin causes PKCα-mediated internalization of erbb2/HER2 tyrosine kinase in breast cancer cells. J Biol Chem. 2019;294:887-901 pubmed publisher
Ashley N, Ouaret D, Bodmer W. Cellular polarity modulates drug resistance in primary colorectal cancers via orientation of the multidrug resistance protein ABCB1. J Pathol. 2019;247:293-304 pubmed publisher
Sonnenschein H, Lawrence K, Wittenberg K, Slykas F, Dohleman E, Knoublauch J, et al. Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and α-actinin, linking innate immunity to the cytoskeleton. FEBS Open Bio. 2018;8:1064-1082 pubmed publisher
Kschonsak Y, Hoffmann I. Activated ezrin controls MISP levels to ensure correct NuMA polarization and spindle orientation. J Cell Sci. 2018;131: pubmed publisher
Röwer C, George C, Reimer T, Stengel B, Radtke A, Gerber B, et al. Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues. Transl Oncol. 2017;11:1-10 pubmed publisher
Schütte M, Risch T, Abdavi Azar N, Boehnke K, Schumacher D, Keil M, et al. Molecular dissection of colorectal cancer in pre-clinical models identifies biomarkers predicting sensitivity to EGFR inhibitors. Nat Commun. 2017;8:14262 pubmed publisher
Demacopulo B, Lema B, Cabrini R, Kreimann E. Similar expression pattern of NHERF1 and EZRIN in papillary but not in solid areas of human serous ovarian carcinomas. Acta Histochem. 2016;118:797-805 pubmed publisher
Pokharel D, Padula M, Lu J, Tacchi J, Luk F, Djordjevic S, et al. Proteome analysis of multidrug-resistant, breast cancer-derived microparticles. J Extracell Vesicles. 2014;3: pubmed publisher
Eißmann M, Schwamb B, Melzer I, Moser J, Siele D, Köhl U, et al. A functional yeast survival screen of tumor-derived cDNA libraries designed to identify anti-apoptotic mammalian oncogenes. PLoS ONE. 2013;8:e64873 pubmed publisher
Fukasawa H, Obayashi H, Schmieder S, Lee J, Ghosh P, Farquhar M. Phosphorylation of podocalyxin (Ser415) Prevents RhoA and ezrin activation and disrupts its interaction with the actin cytoskeleton. Am J Pathol. 2011;179:2254-65 pubmed publisher
Casaletto J, Saotome I, Curto M, McClatchey A. Ezrin-mediated apical integrity is required for intestinal homeostasis. Proc Natl Acad Sci U S A. 2011;108:11924-9 pubmed publisher
Saotome I, Curto M, McClatchey A. Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine. Dev Cell. 2004;6:855-64 pubmed
Pang S, Fang X, Valdman A, Norstedt G, Pousette A, Egevad L, et al. Expression of ezrin in prostatic intraepithelial neoplasia. Urology. 2004;63:609-12 pubmed
Moilanen J, Lassus H, Leminen A, Vaheri A, Butzow R, Carpen O. Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients. Gynecol Oncol. 2003;90:273-81 pubmed
Pang S, Dillner K, Wu X, Pousette A, Norstedt G, Flores Morales A. Gene expression profiling of androgen deficiency predicts a pathway of prostate apoptosis that involves genes related to oxidative stress. Endocrinology. 2002;143:4897-906 pubmed
Allenspach E, Cullinan P, Tong J, Tang Q, Tesciuba A, Cannon J, et al. ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse. Immunity. 2001;15:739-50 pubmed
Knipper M, Richardson G, Mack A, Muller M, Goodyear R, Limberger A, et al. Thyroid hormone-deficient period prior to the onset of hearing is associated with reduced levels of beta-tectorin protein in the tectorial membrane: implication for hearing loss. J Biol Chem. 2001;276:39046-52 pubmed
product information
Product Type :
Antibody
Product Name :
Ezrin Monoclonal Antibody (3C12)
Catalog # :
MA5-13862
Quantity :
500 µL
Price :
US 496.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Bovine, Hamster, Human, Marsupial, Mouse, Non-human primate, Rat
Applications :
Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL
Species :
Bovine, Hamster, Human, Marsupial, Mouse, Non-human primate, Rat
Clone :
3C12
Isotype :
IgG1
Storage :
4° C
Description :
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Immunogen :
A recombinant fragment of human ezrin corresponding to aa 362-585
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL
Aliases :
AW146364; CVIL; CVL; Cytovillin; cytovillin 2; DKFZp762H157; epididymis secretory protein Li 105; ezr; ezr.L; ezr.S; EZRI; Ezrin; ezrin L homeolog; ezrin L homeolog; ezrin S homeolog; FLJ26216; HEL-S-105; MGC1584; p81; R75297; VIL2; villin 2; villin 2 (ezrin); villin-2; XELAEV_18027043mg
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA