P-Glycoprotein Monoclonal Antibody (F4) | Invitrogen MA5-13854 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

P-Glycoprotein Monoclonal Antibody (F4)

catalog :

MA5-13854

quantity :

500 uL

price :

US 470.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

Chinese hamsters, human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; loading ...; fig 4b	Kasendra M, Luc R, Yin J, Manatakis D, Kulkarni G, Lucchesi C, et al. Duodenum Intestine-Chip for preclinical drug assessment in a human relevant model. elife. 2020;9: pubmed publisher
immunocytochemistry; human; 1:25; fig 2i	Clark P, Al Ahmad A, Qian T, Zhang R, Wilson H, Weichert J, et al. Analysis of Cancer-Targeting Alkylphosphocholine Analogue Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model. Mol Pharm. 2016;13:3341-9 pubmed publisher
immunocytochemistry; human; 1:25; fig 2	Stebbins M, Wilson H, Canfield S, Qian T, Palecek S, Shusta E. Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells. Methods. 2016;101:93-102 pubmed publisher
immunocytochemistry; rat	Xu D, Yan M, Li H, Fang P, Liu Y. Influence of P-glycoprotein on brucine transport at the in vitro blood-brain barrier. Eur J Pharmacol. 2012;690:68-76 pubmed publisher
immunocytochemistry; human; 4 ug/ml immunohistochemistry; human; 4 ug/ml	Yamamoto A, Shofuda T, Islam M, Nakamura Y, Yamasaki M, Okano H, et al. ABCB1 is predominantly expressed in human fetal neural stem/progenitor cells at an early development stage. J Neurosci Res. 2009;87:2615-23 pubmed publisher
immunohistochemistry; human	Ozger H, Eralp L, Atalar A, Toker B, Esberk Ateş L, Sungur M, et al. [The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis]. Acta Orthop Traumatol Turc. 2009;43:28-34 pubmed publisher
immunocytochemistry; human	Zhou Y, Li K, Li H. Effect of the novel antipsychotic drug perospirone on P-glycoprotein function and expression in Caco-2 cells. Eur J Clin Pharmacol. 2008;64:697-703 pubmed publisher
western blot; human	Schwock J, Pham N, Cao M, Hedley D. Efficacy of Hsp90 inhibition for induction of apoptosis and inhibition of growth in cervical carcinoma cells in vitro and in vivo. Cancer Chemother Pharmacol. 2008;61:669-81 pubmed
	Daumar P, Goisnard A, Dubois C, Roux M, Depresle M, Penault Llorca F, et al. Chemical biology fluorescent tools for in vitro investigation of the multidrug resistant P-glycoprotein (P-gp) expression in tumor cells. RSC Adv. 2023;13:27016-27035 pubmed publisher
	Mamana J, Humber G, Espinal E, Seo S, Vollmuth N, Sin J, et al. Coxsackievirus B3 infects and disrupts human induced-pluripotent stem cell derived brain-like endothelial cells. Front Cell Infect Microbiol. 2023;13:1171275 pubmed publisher
	Abalenikhina Y, Shchulkin A, Mylnikov P, Rokunov E, Yakusheva E. Mechanisms of P-Glycoprotein Regulation Under Exogenous and Endogenous Oxidative Stress In Vitro. Acta Naturae. 2022;14:69-78 pubmed publisher
	Aniogo E, George B, Abrahamse H. Characterization of resistant MCF-7 breast cancer cells developed by repeated cycles of photodynamic therapy. Front Pharmacol. 2022;13:964141 pubmed publisher
	Pediaditakis I, Kodella K, Manatakis D, Le C, Barthakur S, Sorets A, et al. A microengineered Brain-Chip to model neuroinflammation in humans. iScience. 2022;25:104813 pubmed publisher
	Chai A, Callaghan R, Gelissen I. The Ubiquitin E3 Ligase Nedd4 Regulates the Expression and Amyloid-β Peptide Export Activity of P-Glycoprotein. Int J Mol Sci. 2022;23: pubmed publisher
	Goisnard A, Daumar P, Dubois C, Aubel C, Roux M, Depresle M, et al. LightSpot®-FL-1 Fluorescent Probe: An Innovative Tool for Cancer Drug Resistance Analysis by Direct Detection and Quantification of the P-glycoprotein (P-gp) on Monolayer Culture and Spheroid Triple Negative Breast Cancer Models. Cancers (Basel). 2021;13: pubmed publisher
	Kobori T, Tameishi M, Tanaka C, Urashima Y, Obata T. Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells. PLoS ONE. 2021;16:e0250889 pubmed publisher
	Ji R, Li Y, He C, Zhu X, He A, Lu Y. Detection and analysis of multiple biomarkers in ovarian cancer: clinical significance in diagnosis, treatment, and prognosis evaluation. Gland Surg. 2020;9:2175-2186 pubmed publisher
	Shen P, Cheng Y. Long noncoding RNA lncARSR confers resistance to Adriamycin and promotes osteosarcoma progression. Cell Death Dis. 2020;11:362 pubmed publisher
	Ouyang X, Dong C, Ubogu E. In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves. J Peripher Nerv Syst. 2019;24:195-206 pubmed publisher
	Li Z, Jiang X, Chen P, Wu X, Duan A, Qin Y. Combined effects of octreotide and cisplatin on the proliferation of side population cells from anaplastic thyroid cancer cell lines. Oncol Lett. 2018;16:4033-4042 pubmed publisher
	Palladino S, Helton E, Jain P, Dong C, Crowley M, Crossman D, et al. The Human Blood-Nerve Barrier Transcriptome. Sci Rep. 2017;7:17477 pubmed publisher
	Guan J, Chen X, Zhu H, Luo S, Cao B, Ding L. Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line. World J Gastroenterol. 2004;10:3522-7 pubmed
	Peng Z, Xiao Z, Wang Y, Liu P, Cai Y, Lu S, et al. Reversal of P-glycoprotein-mediated multidrug resistance with small interference RNA (siRNA) in leukemia cells. Cancer Gene Ther. 2004;11:707-12 pubmed
	Novotna M, Libra A, Kopecky M, Pavek P, Fendrich Z, Semecky V, et al. P-glycoprotein expression and distribution in the rat placenta during pregnancy. Reprod Toxicol. 2004;18:785-92 pubmed
	Candeil L, Gourdier I, Peyron D, Vezzio N, Copois V, Bibeau F, et al. ABCG2 overexpression in colon cancer cells resistant to SN38 and in irinotecan-treated metastases. Int J Cancer. 2004;109:848-54 pubmed
	Pavek P, Staud F, Fendrich Z, Sklenarova H, Libra A, Novotna M, et al. Examination of the functional activity of P-glycoprotein in the rat placental barrier using rhodamine 123. J Pharmacol Exp Ther. 2003;305:1239-50 pubmed
	Regina A, Demeule M, Bérubé A, Moumdjian R, Berthelet F, Beliveau R. Differences in multidrug resistance phenotype and matrix metalloproteinases activity between endothelial cells from normal brain and glioma. J Neurochem. 2003;84:316-24 pubmed
	Demeule M, Jodoin J, Beaulieu E, Brossard M, Beliveau R. Dexamethasone modulation of multidrug transporters in normal tissues. FEBS Lett. 1999;442:208-14 pubmed

product information

Product Type :

Antibody

Product Name :

P-Glycoprotein Monoclonal Antibody (F4)

Catalog # :

MA5-13854

Quantity :

500 uL

Price :

US 470.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Hamster, Human

Applications :

Flow Cytometry: 2 ug/test, Immunocytochemistry: 1:10-1:100, Western Blot: 1:10-1:200

Species :

Hamster, Human

Clone :

Isotype :

IgG1

Storage :

4 C

Description :

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

Immunogen :

Human and hamster drug-resistant viable cells followed by crude plasma membranes

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 2 ug/test, Immunocytochemistry: 1:10-1:100, Western Blot: 1:10-1:200

Aliases :

ABC superfamily; P-glycoprotein; ABC20; ABCB1; Abcb1a; Abcb1b; Abcb4; ATP binding cassette subfamily B member 1; ATP-binding cassette sub-family B (MDR/TAP) member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette sub-family B member 1; ATP-binding cassette sub-family B member 1A; ATP-binding cassette sub-family B member 1B; ATP-binding cassette transporter subfamily B, member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1 (P-glycoprotein/multidrug resistance 1); ATP-binding cassette, subfamily B (MDR/TAP), member 1A; ATP-binding cassette, sub-family B (MDR/TAP), member 1A; ATP-binding cassette, subfamily B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 1B; ATP-binding cassette, sub-family B (MDR/TAP), member 4; ATP-binding cassette, subfamily B, member 1A; ATP-binding cassette, subfamily B, member 1B; ATP-dependent translocase ABCB1; ATP-dependent translocase ABCB1; multidrug resistance protein 1; bovine p-glycoprotein; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; ecotropic viral integration site 32; Evi32; GP170; mdr; Mdr1; MDR1A; Mdr1b; Mdr3; mdr-3; multi-drug resistance 3; multidrug resistance p-glycoprotein; multidrug resistance protein 1; Multidrug resistance protein 1A; multidrug resistance protein 1B; Multidrug resistance protein 2; multidrug resistance protein 3; multidrug resistance protein MDR1; multiple drug resistant 1a; P glycoprotein 1; P glycoprotein 3; P-glycoprotein; P-glycoprotein 1; P-glycoprotein 2; P-glycoprotein 3; P-glycoprotein/multidrug resistance 1; Pgp; p-gp; PGP1; PGP2; PGY1; Pgy-1; Pgy1-1; PGY2; Pgy3; Pgy-3; Phospholipid transporter ABCB1

more info or order :

Invitrogen product webpage