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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Cytokeratin 10 Monoclonal Antibody (DE-K10)
catalog :
MA5-13705
quantity :
500 uL
price :
US 464.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DE-K10
reactivity :
human, mouse, rat, dogs, cat, domestic horse
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 55
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 4e
Ashcroft F, Mahammad N, Midtun Flatekvål H, Jullumstrø Feuerherm A, Johansen B. cPLA2α Enzyme Inhibition Attenuates Inflammation and Keratinocyte Proliferation. Biomolecules. 2020;10: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:400; fig 5
Stewart M, Bechberger J, Welch I, Naus C, Laird D. Cx26 knockout predisposes the mammary gland to primary mammary tumors in a DMBA-induced mouse model of breast cancer. Oncotarget. 2015;6:37185-99 pubmed publisher
  • immunohistochemistry - paraffin section; domestic horse; 1:100; fig 1
  • western blot; domestic horse; 1:1000; fig 2
Linardi R, Megee S, Mainardi S, Senoo M, Galantino Homer H. Expression and localization of epithelial stem cell and differentiation markers in equine skin, eye and hoof. Vet Dermatol. 2015;26:213-e47 pubmed publisher
  • immunohistochemistry; human; 1:100
Matsusaki M, Fujimoto K, Shirakata Y, Hirakawa S, Hashimoto K, Akashi M. Development of full-thickness human skin equivalents with blood and lymph-like capillary networks by cell coating technology. J Biomed Mater Res A. 2015;103:3386-96 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 2b
von Neubeck C, Geniza M, Kauer P, Robinson R, Chrisler W, Sowa M. The effect of low dose ionizing radiation on homeostasis and functional integrity in an organotypic human skin model. Mutat Res. 2015;775:10-8 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
van Drongelen V, Danso M, Out J, Mulder A, Lavrijsen A, Bouwstra J, et al. Explant cultures of atopic dermatitis biopsies maintain their epidermal characteristics in vitro. Cell Tissue Res. 2015;361:789-97 pubmed publisher
  • western blot; human; 1:200
Neumann C, Bigliardi Qi M, Widmann C, Bigliardi P. The δ-opioid receptor affects epidermal homeostasis via ERK-dependent inhibition of transcription factor POU2F3. J Invest Dermatol. 2015;135:471-480 pubmed publisher
  • immunohistochemistry; human; fig 1
Ouyang H, Xue Y, Lin Y, Zhang X, Xi L, Patel S, et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature. 2014;511:358-61 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:600; tbl 3
Lindström A, Hellberg D. Immunohistochemical LRIG3 expression in cervical intraepithelial neoplasia and invasive squamous cell cervical cancer: association with expression of tumor markers, hormones, high-risk HPV-infection, smoking and patient outcome. Eur J Histochem. 2014;58:2227 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 6
Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, et al. Phenotypic plasticity in normal breast derived epithelial cells. BMC Cell Biol. 2014;15:20 pubmed publisher
  • immunohistochemistry; human; 1:100
van Drongelen V, Danso M, Mulder A, Mieremet A, Van Smeden J, Bouwstra J, et al. Barrier properties of an N/TERT-based human skin equivalent. Tissue Eng Part A. 2014;20:3041-9 pubmed publisher
  • immunohistochemistry; human
Mitchell D, Israr M, Alam S, Dinello D, Kishel J, Jia R, et al. HIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium. HIV Med. 2014;15:196-202 pubmed publisher
  • immunohistochemistry; rat
Haisma E, Rietveld M, de Breij A, van Dissel J, El Ghalbzouri A, Nibbering P. Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model. PLoS ONE. 2013;8:e82800 pubmed publisher
  • immunohistochemistry; human; 2 ug/ml
von Neubeck C, Shankaran H, Geniza M, Kauer P, Robinson R, Chrisler W, et al. Integrated experimental and computational approach to understand the effects of heavy ion radiation on skin homeostasis. Integr Biol (Camb). 2013;5:1229-43 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 6
Lian X, Selekman J, Bao X, Hsiao C, Zhu K, Palecek S. A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells. PLoS ONE. 2013;8:e60016 pubmed publisher
  • immunohistochemistry; human
Pal S, Sakamoto K, Aragaki T, Akashi T, Yamaguchi A. The expression profiles of acidic epithelial keratins in ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013;115:523-31 pubmed publisher
  • immunocytochemistry; human
Moffatt Jauregui C, Robinson B, de Moya A, Brockman R, Roman A, Cash M, et al. Establishment and characterization of a telomerase immortalized human gingival epithelial cell line. J Periodontal Res. 2013;48:713-21 pubmed publisher
  • immunohistochemistry; human; 1:200
Kretz M, Siprashvili Z, Chu C, Webster D, Zehnder A, Qu K, et al. Control of somatic tissue differentiation by the long non-coding RNA TINCR. Nature. 2013;493:231-5 pubmed publisher
  • immunohistochemistry; human; 1:50
Commandeur S, van Drongelen V, de Gruijl F, El Ghalbzouri A. Epidermal growth factor receptor activation and inhibition in 3D in vitro models of normal skin and human cutaneous squamous cell carcinoma. Cancer Sci. 2012;103:2120-6 pubmed publisher
  • western blot; human
Yang F, Waters K, Webb Robertson B, Sowa M, von Neubeck C, Aldrich J, et al. Quantitative phosphoproteomics identifies filaggrin and other targets of ionizing radiation in a human skin model. Exp Dermatol. 2012;21:352-7 pubmed publisher
  • immunohistochemistry; human; 1:500
Lara M, Gonzalez Gonzalez E, Speaker T, Hickerson R, Leake D, Milstone L, et al. Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays. Hum Gene Ther. 2012;23:816-23 pubmed publisher
  • immunohistochemistry; human
Mitchell D, Israr M, Alam S, Kishel J, Dinello D, Meyers C. Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium. HIV Med. 2012;13:276-90 pubmed publisher
  • immunocytochemistry; human
Alexaki V, Pelekanou V, Notas G, Venihaki M, Kampa M, Dessirier V, et al. B-cell maturation antigen (BCMA) activation exerts specific proinflammatory effects in normal human keratinocytes and is preferentially expressed in inflammatory skin pathologies. Endocrinology. 2012;153:739-49 pubmed publisher
  • immunohistochemistry; human
Li B, Li J, Devlin B, Markert M. Thymic microenvironment reconstitution after postnatal human thymus transplantation. Clin Immunol. 2011;140:244-59 pubmed publisher
  • immunohistochemistry; human
Sakamoto K, Aragaki T, Morita K, Kawachi H, Kayamori K, Nakanishi S, et al. Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis. Histopathology. 2011;58:531-42 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Obarzanek Fojt M, Favre B, Kypriotou M, Ryser S, Huber M, Hohl D. Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes. Eur J Cell Biol. 2011;90:279-90 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Obarzanek Fojt M, Favre B, Huber M, Ryser S, Moodycliffe A, Wipff P, et al. Induction of p38, tumour necrosis factor-? and RANTES by mechanical stretching of keratinocytes expressing mutant keratin 10R156H. Br J Dermatol. 2011;164:125-34 pubmed publisher
  • immunohistochemistry; human
Israr M, Mitchell D, Alam S, Dinello D, Kishel J, Meyers C. The HIV protease inhibitor lopinavir/ritonavir (Kaletra) alters the growth, differentiation and proliferation of primary gingival epithelium. HIV Med. 2011;12:145-56 pubmed publisher
  • immunocytochemistry; human
  • immunohistochemistry; human; 1:350
Sen G, Reuter J, Webster D, Zhu L, Khavari P. DNMT1 maintains progenitor function in self-renewing somatic tissue. Nature. 2010;463:563-7 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:600; tbl 1
Samir R, Asplund A, Tot T, Pekar G, Hellberg D. Tissue tumor marker expression in smokers, including serum cotinine concentrations, in women with cervical intraepithelial neoplasia or normal squamous cervical epithelium. Am J Obstet Gynecol. 2010;202:579.e1-7 pubmed publisher
  • immunohistochemistry; human
Metallo C, Azarin S, Moses L, Ji L, de Pablo J, Palecek S. Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs. Tissue Eng Part A. 2010;16:213-23 pubmed publisher
  • immunohistochemistry; human; 1:100
Charest J, Jennings J, King W, Kowalczyk A, Garcia A. Cadherin-mediated cell-cell contact regulates keratinocyte differentiation. J Invest Dermatol. 2009;129:564-72 pubmed publisher
  • immunohistochemistry; human
Markert M, Li J, Devlin B, Hoehner J, Rice H, Skinner M, et al. Use of allograft biopsies to assess thymopoiesis after thymus transplantation. J Immunol. 2008;180:6354-64 pubmed
  • immunohistochemistry; human; 1:50
Lalli A, Tilakaratne W, Ariyawardana A, Fitchett C, Leigh I, Hagi Pavli E, et al. An altered keratinocyte phenotype in oral submucous fibrosis: correlation of keratin K17 expression with disease severity. J Oral Pathol Med. 2008;37:211-20 pubmed publisher
Hernandez B, Skiba N, Pl xf6 ssl K, Strain M, Liu Y, Grigsby D, et al. Polarized Desmosome and Hemidesmosome Shedding via Small Extracellular Vesicles is an Early Indicator of Outer Blood-Retina Barrier Dysfunction. J Extracell Biol. 2023;2: pubmed publisher
Yin W, Egawa N, Zheng K, Griffin H, Tian P, Aiyenuro A, et al. HPV E6 inhibits E6AP to regulate epithelial homeostasis by modulating keratinocyte differentiation commitment and YAP1 activation. PLoS Pathog. 2023;19:e1011464 pubmed publisher
Li S, Lee D, Kim H, Kim J, Jung Y, Jung H. Unraveled roles of Cav1.2 in proliferation and stemness of ameloblastoma. Cell Biosci. 2022;12:145 pubmed publisher
Tsirvouli E, Ashcroft F, Johansen B, Kuiper M. Logical and experimental modeling of cytokine and eicosanoid signaling in psoriatic keratinocytes. iScience. 2021;24:103451 pubmed publisher
Dobrokhotov O, Sunagawa M, Torii T, Mii S, Kawauchi K, Enomoto A, et al. Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells. Front Cell Dev Biol. 2021;9:728383 pubmed publisher
Montero A, Qu xed lez C, Valencia L, Gir xf3 n P, Jorcano J, Velasco D. Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents. Int J Mol Sci. 2021;22: pubmed publisher
Choi D, Jeon B, Lim M, Lee D, Ye S, Jeong S, et al. 3D cell culture using a clinostat reproduces microgravity-induced skin changes. NPJ Microgravity. 2021;7:20 pubmed publisher
Jackson R, Maarsingh J, Herbst Kralovetz M, Van Doorslaer K. 3D Oral and Cervical Tissue Models for Studying Papillomavirus Host-Pathogen Interactions. Curr Protoc Microbiol. 2020;59:e129 pubmed publisher
Kim J, Villadsen R. The Expression Pattern of Epidermal Differentiation Marker Keratin 10 in the Normal Human Breast and Breast Cancer Cells. J Histochem Cytochem. 2020;68:561-570 pubmed publisher
Kashgari G, Meinecke L, Gordon W, Ruiz B, Yang J, Ma A, et al. Epithelial Migration and Non-adhesive Periderm Are Required for Digit Separation during Mammalian Development. Dev Cell. 2020;52:764-778.e4 pubmed publisher
Hogervorst M, Rietveld M, de Gruijl F, El Ghalbzouri A. A shift from papillary to reticular fibroblasts enables tumour-stroma interaction and invasion. Br J Cancer. 2018;118:1089-1097 pubmed publisher
Mehta F, Son J, Hewitt S, Jang E, Lydon J, Korach K, et al. Distinct functions and regulation of epithelial progesterone receptor in the mouse cervix, vagina, and uterus. Oncotarget. 2016;7:17455-67 pubmed publisher
Mugita Y, Minematsu T, Huang L, Nakagami G, Kishi C, Ichikawa Y, et al. Histopathology of Incontinence-Associated Skin Lesions: Inner Tissue Damage Due to Invasion of Proteolytic Enzymes and Bacteria in Macerated Rat Skin. PLoS ONE. 2015;10:e0138117 pubmed publisher
Hughes M, Jiang T, Lin S, Leung Y, Kobielak K, Widelitz R, et al. Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis. J Invest Dermatol. 2014;134:24-32 pubmed publisher
Fukui D, Bando G, Ishikawa Y, Kadota K. Adenosquamous carcinoma with cilium formation, mucin production and keratinization in the nasal cavity of a red fox (Vulpes vulpes schrencki). J Comp Pathol. 2007;137:142-5 pubmed
Aho S. Plakin proteins are coordinately cleaved during apoptosis but preferentially through the action of different caspases. Exp Dermatol. 2004;13:700-7 pubmed
Chang Y, Liu H, Wang W, Lee D, Tsai S. A study of cytokeratin profiles in localized cutaneous amyloids. Arch Dermatol Res. 2004;296:83-8 pubmed
Apaydin R, Gurbuz Y, Bayramgurler D, Muezzinoglu B, Bilen N. Cytokeratin expression in lichen amyloidosus and macular amyloidosis. J Eur Acad Dermatol Venereol. 2004;18:305-9 pubmed
Hale L, Buckley R, Puck J, Patel D. Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency. Clin Immunol. 2004;110:63-70 pubmed
Hale L, Markert M. Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus. J Immunol. 2004;172:617-24 pubmed
Sanchez Carbayo M, Socci N, Charytonowicz E, Lu M, Prystowsky M, Childs G, et al. Molecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes. Cancer Res. 2002;62:6973-80 pubmed
product information
Product Type :
Antibody
Product Name :
Cytokeratin 10 Monoclonal Antibody (DE-K10)
Catalog # :
MA5-13705
Quantity :
500 uL
Price :
US 464.00
Clonality :
Monoclonal
Purity :
Protein A/G
Host :
Mouse
Reactivity :
Canine, Feline, Human, Rat
Applications :
Immunohistochemistry (Paraffin): 0.5-1.0 ug/mL, Western Blot: 2 ug/mL
Species :
Canine, Feline, Human, Rat
Clone :
DE-K10
Isotype :
IgG1, kappa
Storage :
4 C
Description :
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e., skin) and non-keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8. 5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
Immunogen :
Cytoskeletal preparation extracted from human ectocervical epithelium
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 0.5-1.0 ug/mL, Western Blot: 2 ug/mL
Aliases :
56 kDa cytokeratin; BCIE; BIE; CK10; CK-10; CK-17; cytokeratin 10; cytokeratin-10; cytokeratin-17; D130054E02Rik; EHK; epidermal keratin 10; epithelial keratin 10; intermediate filament protein; K10; K1C1; Ka10; Ker10; keratin 10; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin complex 1, acidic, gene 10; keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 59 kDa; keratin-10; keratin-17; KPP; Krt-1.10; KRT10; krt10 {ECO:0000250; Krt1-10; suprabasal cytokeratin 10; type I keratin KA10; UniProtKB:P13645}
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA