MMP2 Monoclonal Antibody (CA-4001 (CA719E3C)) | Invitrogen MA5-13590 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MMP2 Monoclonal Antibody (CA-4001 (CA719E3C))

catalog :

MA5-13590

quantity :

500 µL

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CA-4001 (CA719E3C)

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 37

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 4b	Yi R, Zhang J, Sun P, Qian Y, Zhao X. Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice. Molecules. 2019;24: pubmed publisher
immunohistochemistry; human; 1:50	Guan H, Tan J, Zhang F, Gao L, Bai L, Qi D, et al. Myofibroblasts from salivary gland adenoid cystic carcinomas promote cancer invasion by expressing MMP2 and CXCL12. Histopathology. 2015;66:781-90 pubmed publisher
immunohistochemistry; human; 0.3:250	Costescu M, Coman O, Tampa M, Tudose I, Coman L, Georgescu S. Axillary basal cell carcinoma--a rare form of a frequent kind of carcinoma. Rom J Morphol Embryol. 2013;54:851-6 pubmed
immunohistochemistry; chicken	Sevimli A, Yagci A, Bulbul A. Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy. Biotech Histochem. 2013;88:336-44 pubmed publisher
immunohistochemistry; human	Puff C, Risha E, Baumgartner W. Regression of canine cutaneous histiocytoma is associated with an orchestrated expression of matrix metalloproteinases. J Comp Pathol. 2013;149:208-15 pubmed publisher
immunohistochemistry; human; 1:250	Beltran E, Matiasek K, De Risio L, De Stefani A, Feliu Pascual A, Matiasek L. Expression of MMP-2 and MMP-9 in benign canine rostrotentorial meningiomas is not correlated to the extent of peritumoral edema. Vet Pathol. 2013;50:1091-8 pubmed publisher
western blot; mouse	Guo L, Chen C, Shi M, Wang F, Chen X, Diao D, et al. Stat3-coordinated Lin-28-let-7-HMGA2 and miR-200-ZEB1 circuits initiate and maintain oncostatin M-driven epithelial-mesenchymal transition. Oncogene. 2013;32:5272-82 pubmed publisher
western blot; mouse; 5 ug/ml	Barczyk M, Lu N, Popova S, Bolstad A, Gullberg D. ?11?1 integrin-mediated MMP-13-dependent collagen lattice contraction by fibroblasts: evidence for integrin-coordinated collagen proteolysis. J Cell Physiol. 2013;228:1108-19 pubmed publisher
immunohistochemistry; human	Kim J, Kim H, Koo B, Rha K, Yoon Y. Expression of matrix metalloproteinase-12 is correlated with extracapsular spread of tumor from nodes with metastasis in head and neck squamous cell carcinoma. Eur Arch Otorhinolaryngol. 2013;270:1137-42 pubmed publisher
blocking or activating experiments; human; 4 ug/ml	Yamamoto N, Bryce N, Metzler Nolte N, Hambley T. Effects of enzymatic activation on the distribution of fluorescently tagged MMP-2 cleavable peptides in cancer cells and spheroids. Bioconjug Chem. 2012;23:1110-8 pubmed publisher
immunohistochemistry; rat; 1:100	Ma J, Mercer R, Barger M, Schwegler Berry D, Scabilloni J, MA J, et al. Induction of pulmonary fibrosis by cerium oxide nanoparticles. Toxicol Appl Pharmacol. 2012;262:255-64 pubmed publisher
immunohistochemistry; human; 1:25	Omran O, Thabet M. Gelatinases A and B expression in human colorectal cancer in upper Egypt: a clinicopathological study. Ultrastruct Pathol. 2012;36:108-16 pubmed publisher
western blot; rat	Sun M, Chen K, Tsao Y, Kao L, Han W, Lin K, et al. Down-regulation of matrix metalloproteinase-9 by pyrrolidine dithiocarbamate prevented retinal ganglion cell death after transection of optic nerve in rats. Curr Eye Res. 2011;36:1053-63 pubmed publisher
immunohistochemistry; human; 1:100	Mallis A, Teymoortash A, Mastronikolis N, Werner J, Papadas T. MMP-2 expression in 102 patients with glottic laryngeal cancer. Eur Arch Otorhinolaryngol. 2012;269:639-42 pubmed publisher
immunohistochemistry; rat; 1:100	Ucar E, Borazan A, Semerci E, Binici D, Yaldiz M, Aysal A, et al. The effects of interferon alpha2b on chemically-induced peritoneal fibrosis and on peritoneal tissue MMP-2 and TIMP-2 levels in rats. J Int Med Res. 2010;38:187-94 pubmed
western blot; mouse; fig 3a	Seo J, Kim T, Leem Y, Lee K, Park S, Baek I, et al. SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice. Brain Res. 2009;1261:100-8 pubmed publisher
western blot; human; 1:400	He Q, Pang R, Song X, Chen J, Chen H, Chen B, et al. Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARgamma Dependent and Independent Mechanisms. PPAR Res. 2008;2008:649808 pubmed publisher
immunocytochemistry; human; 1:50	Ullmann U, Gilles C, De Rycke M, Van de Velde H, Sermon K, Liebaers I. GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions. Mol Hum Reprod. 2008;14:169-79 pubmed publisher
western blot; human; 1:500	Ou D, Tao Y, Tang F, Yang L. The hepatitis B virus X protein promotes hepatocellular carcinoma metastasis by upregulation of matrix metalloproteinases. Int J Cancer. 2007;120:1208-14 pubmed
blocking or activating experiments; chicken	Linask K, Han M, Cai D, Brauer P, Maisastry S. Cardiac morphogenesis: matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping. Dev Dyn. 2005;233:739-53 pubmed
immunohistochemistry; rat; 1:100	Scabilloni J, Wang L, Antonini J, Roberts J, Castranova V, Mercer R. Matrix metalloproteinase induction in fibrosis and fibrotic nodule formation due to silica inhalation. Am J Physiol Lung Cell Mol Physiol. 2005;288:L709-17 pubmed
	Abdelkader N, Elbaset M, Moustafa P, Ibrahim S. Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling. Arch Pharm Res. 2022;45:475-493 pubmed publisher
	Karagülle O, Yurttas A. Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line. Rev Assoc Med Bras (1992). 2022;68:507-513 pubmed publisher
	Wang L, Zhang Z, Wang L, Wei H, Zhang T, Zhang L, et al. TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways. Cell Death Dis. 2022;13:472 pubmed publisher
	Gajda Morszewski P, Gurgul I, Janczy Cempa E, Mazuryk O, x141 omzik M, Brindell M. Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents. Pharmaceuticals (Basel). 2021;14: pubmed publisher
	Huang S, Sulistyowati E, Chao Y, Wu B, Dai Z, Hsu J, et al. In Vitro Evaluation of the Anti-Inflammatory Effect of KMUP-1 and In Vivo Analysis of Its Therapeutic Potential in Osteoarthritis. Biomedicines. 2021;9: pubmed publisher
	Custers L, Khabirova E, Coorens T, Oliver T, Calandrini C, Young M, et al. Somatic mutations and single-cell transcriptomes reveal the root of malignant rhabdoid tumours. Nat Commun. 2021;12:1407 pubmed publisher
	Celentano A, Yap T, Paolini R, Yiannis C, Mirams M, Koo K, et al. Inhibition of matrix metalloproteinase-2 modulates malignant behaviour of oral squamous cell carcinoma cells. J Oral Pathol Med. 2021;50:323-332 pubmed publisher
	Rhee H, Han M, Kim S, Kim I, Lee H, Bae S, et al. The expression of two isoforms of matrix metalloproteinase-2 in aged mouse models of diabetes mellitus and chronic kidney disease. Kidney Res Clin Pract. 2018;37:222-229 pubmed publisher
	Pellikainen J, Ropponen K, Kataja V, Kellokoski J, Eskelinen M, Kosma V. Expression of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer with a special reference to activator protein-2, HER2, and prognosis. Clin Cancer Res. 2004;10:7621-8 pubmed
	Andersen T, del Carmen Ovejero M, Kirkegaard T, Lenhard T, Foged N, Delaisse J. A scrutiny of matrix metalloproteinases in osteoclasts: evidence for heterogeneity and for the presence of MMPs synthesized by other cells. Bone. 2004;35:1107-19 pubmed
	Gaiotto M, Focchi J, Ribalta J, Stavale J, Baracat E, Lima G, et al. Comparative study of MMP-2 (matrix metalloproteinase 2) immune expression in normal uterine cervix, intraepithelial neoplasias, and squamous cells cervical carcinoma. Am J Obstet Gynecol. 2004;190:1278-82 pubmed
	Bovo A, da Silva I, Takita L, Fochi J, Stavale J, Marks G, et al. A comparative study of MMP-2 in vulvar neoplasms. Gynecol Oncol. 2004;93:454-7 pubmed
	Kirkegaard T, Hansen A, Bruun E, Brynskov J. Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease. Gut. 2004;53:701-9 pubmed
	Alami J, Williams B, Yeger H. Derivation and characterization of a Wilms' tumour cell line, WiT 49. Int J Cancer. 2003;107:365-74 pubmed
	Miao Q, Baumgartner W, Failing K, Alldinger S. Phase-dependent expression of matrix metalloproteinases and their inhibitors in demyelinating canine distemper encephalitis. Acta Neuropathol. 2003;106:486-94 pubmed
	Ratzinger G, Stoitzner P, Ebner S, Lutz M, Layton G, Rainer C, et al. Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin. J Immunol. 2002;168:4361-71 pubmed

product information

Product Type :

Antibody

Product Name :

MMP2 Monoclonal Antibody (CA-4001 (CA719E3C))

Catalog # :

MA5-13590

Quantity :

500 µL

Price :

US 450.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunohistochemistry (Paraffin): Assay-dependent, Inhibition Assays: 2-4 µg/mL, Western Blot: 0.5-1.0 µg/mL

Species :

Human, Mouse, Rat

Clone :

CA-4001 (CA719E3C)

Isotype :

IgG1

Storage :

4° C

Description :

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

Immunogen :

N-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): Assay-dependent, Inhibition Assays: 2-4 µg/mL, Western Blot: 0.5-1.0 µg/mL

Aliases :

72 kDa gelatinase; 72 kDa type IV collagenase; 72 kDa type IV collagenase isoform a preproprotein; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; CLG4; CLG4A; Collagenase; collagenase type IV-A; endopeptidase; GelA; gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloprotease 2; matrix metalloproteinase; matrix metalloproteinase 2; matrix metalloproteinase 2 (72 KDa type IV collagenase); matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP2; MMP-2; MMP-2 protein; MMP-II; MMPs; MONA; neutrophil gelatinase; PEX; prepro-72kDa matrix metalloproteinase; Progelatinase A; TBE1; TBE-1; wu:fa99h12; wu:fk89d01