CD8 alpha Monoclonal Antibody (C8/144B) | Invitrogen MA5-13473 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD8 alpha Monoclonal Antibody (C8/144B)

catalog :

MA5-13473

quantity :

200 µL

price :

US 499.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C8/144B

reactivity :

human, mouse

application :

immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 53

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:50; fig 1	Azzimonti B, Raimondo L, Squarzanti D, Rosso T, Zanetta P, Aluffi Valletti P, et al. Macrophages expressing TREM-1 are involved in the progression of HPV16-related oropharyngeal squamous cell carcinoma. Ann Med. 2021;53:541-550 pubmed publisher
immunohistochemistry; mouse; 1:50; loading ...; fig 1c	Khosravi Maharlooei M, Li H, Hoelzl M, Zhao G, Ruiz A, Misra A, et al. Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice. J Autoimmun. 2021;119:102612 pubmed publisher
immunohistochemistry; human; fig 4g	Liu X, Kong W, Peterson C, McGrail D, Hoang A, Zhang X, et al. PBRM1 loss defines a nonimmunogenic tumor phenotype associated with checkpoint inhibitor resistance in renal carcinoma. Nat Commun. 2020;11:2135 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; loading ...; fig s4a	Cassetta L, Fragkogianni S, Sims A, Swierczak A, Forrester L, Zhang H, et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell. 2019;35:588-602.e10 pubmed publisher
immunohistochemistry; mouse; fig 6b	Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, et al. Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse. Clin Cancer Res. 2017;23:149-158 pubmed publisher
immunohistochemistry - paraffin section; human; 1:25; fig 1	Chen P, Roh W, Reuben A, Cooper Z, Spencer C, Prieto P, et al. Analysis of Immune Signatures in Longitudinal Tumor Samples Yields Insight into Biomarkers of Response and Mechanisms of Resistance to Immune Checkpoint Blockade. Cancer Discov. 2016;6:827-37 pubmed publisher
immunohistochemistry - paraffin section; human	Zhao L, Li C, Jin P, Ng C, Lin Z, Li Y, et al. Histopathological features of sinonasal inverted papillomas in chinese patients. Laryngoscope. 2016;126:E141-7 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2	Gao T, Ng C, Li C, Li Y, Duan C, Shen L, et al. Smoking is an independent association of squamous metaplasia in Chinese nasal polyps. Int Forum Allergy Rhinol. 2016;6:66-74 pubmed publisher
immunoprecipitation; human; fig 4	Li G, Nguyen C, Ryckman B, Britt W, Kamil J. A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism. Proc Natl Acad Sci U S A. 2015;112:4471-6 pubmed publisher
immunohistochemistry - paraffin section; human; 1:20	Torres Cabala C, Curry J, Li Ning Tapia E, Ramos C, Tetzlaff M, Prieto V, et al. HTLV-1-associated infective dermatitis demonstrates low frequency of FOXP3-positive T-regulatory lymphocytes. J Dermatol Sci. 2015;77:150-5 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; fig 2	Hermans C, Anz D, Engel J, Kirchner T, Endres S, Mayr D. Analysis of FoxP3+ T-regulatory cells and CD8+ T-cells in ovarian carcinoma: location and tumor infiltration patterns are key prognostic markers. PLoS ONE. 2014;9:e111757 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Azzimonti B, Zavattaro E, Provasi M, Vidali M, Conca A, Catalano E, et al. Intense Foxp3+ CD25+ regulatory T-cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8+/Foxp3+ CD25+ ratio. Br J Dermatol. 2015;172:64-73 pubmed publisher
immunohistochemistry; human; 1:20	McQuitty E, Curry J, Tetzlaff M, Prieto V, Duvic M, Torres Cabala C. The differential diagnosis of CD8-positive ("type D") lymphomatoid papulosis. J Cutan Pathol. 2014;41:88-100 pubmed publisher
immunohistochemistry; human	Erickson J, Gilchuk P, Hastings A, Tollefson S, Johnson M, Downing M, et al. Viral acute lower respiratory infections impair CD8+ T cells through PD-1. J Clin Invest. 2012;122:2967-82 pubmed publisher
immunohistochemistry; human; 1:100; fig 5I	Ruffell B, Au A, Rugo H, Esserman L, Hwang E, Coussens L. Leukocyte composition of human breast cancer. Proc Natl Acad Sci U S A. 2012;109:2796-801 pubmed publisher
immunohistochemistry; human	Chang A, Henderson S, Brandt D, Liu N, Guttikonda R, Hsieh C, et al. In situ B cell-mediated immune responses and tubulointerstitial inflammation in human lupus nephritis. J Immunol. 2011;186:1849-60 pubmed publisher
immunohistochemistry; human	Roguedas A, Pers J, Lemasson G, Devauchelle V, Tobon G, Saraux A, et al. Memory B-cell aggregates in skin biopsy are diagnostic for primary Sjögren's syndrome. J Autoimmun. 2010;35:241-7 pubmed publisher
immunohistochemistry; mouse	Chen C, Yeh Y, Wu G, Huang Y, Lai W, Liu J, et al. Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging. Reproduction. 2010;140:105-12 pubmed publisher
immunohistochemistry; human; 1:50	Milani V, Stangl S, Issels R, Gehrmann M, Wagner B, Hube K, et al. Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report. J Transl Med. 2009;7:50 pubmed publisher
immunohistochemistry; human	Zurac S, Girtan M, Lavric L, Petsakos G, Staniceanu F, Bastian A, et al. Local immune alterations in oral mucosa of the edentulous patients; possible cause of teeth loss?. Rom J Intern Med. 2008;46:249-53 pubmed
flow cytometry; human immunohistochemistry; human	Harlin H, Meng Y, Peterson A, Zha Y, Tretiakova M, Slingluff C, et al. Chemokine expression in melanoma metastases associated with CD8+ T-cell recruitment. Cancer Res. 2009;69:3077-85 pubmed publisher
immunohistochemistry; human	Pers J, Devauchelle V, Daridon C, Bendaoud B, Le Berre R, Bordron A, et al. BAFF-modulated repopulation of B lymphocytes in the blood and salivary glands of rituximab-treated patients with Sjögren's syndrome. Arthritis Rheum. 2007;56:1464-77 pubmed
immunohistochemistry; human	Sharma P, Shen Y, Wen S, Yamada S, Jungbluth A, Gnjatic S, et al. CD8 tumor-infiltrating lymphocytes are predictive of survival in muscle-invasive urothelial carcinoma. Proc Natl Acad Sci U S A. 2007;104:3967-72 pubmed
immunohistochemistry; human	Sato E, Olson S, Ahn J, Bundy B, Nishikawa H, Qian F, et al. Intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio are associated with favorable prognosis in ovarian cancer. Proc Natl Acad Sci U S A. 2005;102:18538-43 pubmed
immunohistochemistry; human	Avunduk A, Avunduk M, Pazarli H, Oguz V, Varnell E, Kaufman H, et al. Immunohistochemical analysis of orbital connective tissue specimens of patients with active Graves ophthalmopathy. Curr Eye Res. 2005;30:631-8 pubmed
	Quigley L, Pang L, Tavancheh E, Ernst M, Behren A, Huynh J, et al. Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry. STAR Protoc. 2023;4:101961 pubmed publisher
	Johnson B, Creason A, Stommel J, Keck J, Parmar S, Betts C, et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med. 2022;3:100525 pubmed publisher
	Klein O, Kee D, Gao B, Markman B, Da Gama Duarte J, Quigley L, et al. Combination immunotherapy with nivolumab and ipilimumab in patients with rare gynecological malignancies: results of the CA209-538 clinical trial. J Immunother Cancer. 2021;9: pubmed publisher
	Wang J, Browne L, Slapetova I, Shang F, Lee K, Lynch J, et al. Multiplexed immunofluorescence identifies high stromal CD68+PD-L1+ macrophages as a predictor of improved survival in triple negative breast cancer. Sci Rep. 2021;11:21608 pubmed publisher
	Teng C, Wang T, Shih F, Shyu W, Jeng L. Therapeutic efficacy of dendritic cell vaccine combined with programmed death 1 inhibitor for hepatocellular carcinoma. J Gastroenterol Hepatol. 2021;36:1988-1996 pubmed publisher
	Teng C, Li T, Wang T, Liao D, Wen Y, Wu T, et al. Increased infiltration of regulatory T cells in hepatocellular carcinoma of patients with hepatitis B virus pre-S2 mutant. Sci Rep. 2021;11:1136 pubmed publisher
	Herrera M, Mezheyeuski A, Villabona L, Corvigno S, Strell C, Klein C, et al. Prognostic Interactions between FAP+ Fibroblasts and CD8a+ T Cells in Colon Cancer. Cancers (Basel). 2020;12: pubmed publisher
	Teng C, Wang T, Wu T, Lin J, Shih F, Shyu W, et al. Combination therapy with dendritic cell vaccine and programmed death ligand 1 immune checkpoint inhibitor for hepatocellular carcinoma in an orthotopic mouse model. Ther Adv Med Oncol. 2020;12:1758835920922034 pubmed publisher
	Mitra A, Andrews M, Roh W, De Macedo M, Hudgens C, Carapeto F, et al. Spatially resolved analyses link genomic and immune diversity and reveal unfavorable neutrophil activation in melanoma. Nat Commun. 2020;11:1839 pubmed publisher
	Hawkshaw N, Pilkington S, Murphy S, Al Gazaq N, Farrar M, Watson R, et al. UV radiation recruits CD4+GATA3+ and CD8+GATA3+ T cells while altering the lipid microenvironment following inflammatory resolution in human skin in vivo. Clin Transl Immunology. 2020;9:e01104 pubmed publisher
	Lundgren S, Elebro J, Heby M, Nodin B, Leandersson K, Micke P, et al. Quantitative, qualitative and spatial analysis of lymphocyte infiltration in periampullary and pancreatic adenocarcinoma. Int J Cancer. 2020;146:3461-3473 pubmed publisher
	Werneburg S, Jung J, Kunjamma R, Ha S, Luciano N, Willis C, et al. Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating Disease. Immunity. 2020;52:167-182.e7 pubmed publisher
	Reddy S, Reuben A, Barua S, Jiang H, Zhang S, Wang L, et al. Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer. Cancer Immunol Res. 2019;7:1025-1035 pubmed publisher
	Lane R, Femel J, Breazeale A, Loo C, Thibault G, Kaempf A, et al. IFNγ-activated dermal lymphatic vessels inhibit cytotoxic T cells in melanoma and inflamed skin. J Exp Med. 2018;215:3057-3074 pubmed publisher
	Ganesh S, Shui X, Craig K, Park J, Wang W, Brown B, et al. RNAi-Mediated β-Catenin Inhibition Promotes T Cell Infiltration and Antitumor Activity in Combination with Immune Checkpoint Blockade. Mol Ther. 2018;26:2567-2579 pubmed publisher
	Xue D, Xia T, Wang J, Chong M, Wang S, Zhang C. Role of regulatory T cells and CD8+ T lymphocytes in the dissemination of circulating tumor cells in primary invasive breast cancer. Oncol Lett. 2018;16:3045-3053 pubmed publisher
	Barua S, Solis L, Parra E, Uraoka N, Jiang M, Wang H, et al. A Functional Spatial Analysis Platform for Discovery of Immunological Interactions Predictive of Low-Grade to High-Grade Transition of Pancreatic Intraductal Papillary Mucinous Neoplasms. Cancer Inform. 2018;17:1176935118782880 pubmed publisher
	Stover D, Gil Del Alcazar C, Brock J, Guo H, Overmoyer B, Balko J, et al. Phase II study of ruxolitinib, a selective JAK1/2 inhibitor, in patients with metastatic triple-negative breast cancer. NPJ Breast Cancer. 2018;4:10 pubmed publisher
	Parra E, Uraoka N, Jiang M, Cook P, Gibbons D, Forget M, et al. Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues. Sci Rep. 2017;7:13380 pubmed publisher
	Kawakami F, Sircar K, Rodriguez Canales J, Fellman B, Urbauer D, Tamboli P, et al. Programmed cell death ligand 1 and tumor-infiltrating lymphocyte status in patients with renal cell carcinoma and sarcomatoid dedifferentiation. Cancer. 2017;123:4823-4831 pubmed publisher
	Reuben A, Spencer C, Prieto P, Gopalakrishnan V, Reddy S, Miller J, et al. Genomic and immune heterogeneity are associated with differential responses to therapy in melanoma. NPJ Genom Med. 2017;2: pubmed publisher
	Schweiger T, Berghoff A, Glogner C, Glueck O, Rajky O, Traxler D, et al. Tumor-infiltrating lymphocyte subsets and tertiary lymphoid structures in pulmonary metastases from colorectal cancer. Clin Exp Metastasis. 2016;33:727-39 pubmed publisher
	Shanmugasundaram U, Hilton J, Critchfield J, Greenblatt R, Giudice L, Averbach S, et al. Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium. Am J Reprod Immunol. 2016;76:137-48 pubmed publisher
	Leonard B, Starrett G, Maurer M, Oberg A, Van Bockstal M, Van Dorpe J, et al. APOBEC3G Expression Correlates with T-Cell Infiltration and Improved Clinical Outcomes in High-grade Serous Ovarian Carcinoma. Clin Cancer Res. 2016;22:4746-55 pubmed publisher
	Koyama S, Akbay E, Li Y, Aref A, Skoulidis F, Herter Sprie G, et al. STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment. Cancer Res. 2016;76:999-1008 pubmed publisher
	Dayrit J, Wang W, Goh S, Ramdial P, Lazar A, Calonje E. T-cell-rich angiomatoid polypoid pseudolymphoma of the skin: a clinicopathologic study of 17 cases and a proposed nomenclature. J Cutan Pathol. 2011;38:475-82 pubmed publisher
	Voehringer D, Liang H, Locksley R. Homeostasis and effector function of lymphopenia-induced "memory-like" T cells in constitutively T cell-depleted mice. J Immunol. 2008;180:4742-53 pubmed
	Avunduk M, Avunduk A, Oztekin E, Baltaci A, Ozyazgan Y, Mogolkoc R. Etanercept treatment in the endotoxin-induced uveitis of rats. Exp Eye Res. 2004;79:357-65 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human tonsil stained with CD8 antibody using peroxidase-conjugate and DAB chromogen. Note membrane staining of T cells.

product information

Product Type :

Antibody

Product Name :

CD8 alpha Monoclonal Antibody (C8/144B)

Catalog # :

MA5-13473

Quantity :

200 µL

Price :

US 499.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1:2, Immunohistochemistry (Paraffin): 1:25-1:50

Species :

Human

Clone :

C8/144B

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Immunogen :

A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:2, Immunohistochemistry (Paraffin): 1:25-1:50

Aliases :

BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

more info or order :

Invitrogen product webpage