SNRPB Monoclonal Antibody (Y12) | Invitrogen MA5-13449 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SNRPB Monoclonal Antibody (Y12)

catalog :

MA5-13449

quantity :

500 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Y12

reactivity :

hamsters, Pacific oyster, human, mouse, rat, pigs , fruit fly , domestic rabbit, roundworm

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...; fig 1a	Malkiel S, Jeganathan V, Wolfson S, Manjarrez Orduno N, Marasco E, Aranow C, et al. Checkpoints for Autoreactive B Cells in the Peripheral Blood of Lupus Patients Assessed by Flow Cytometry. Arthritis Rheumatol. 2016;68:2210-20 pubmed publisher
immunohistochemistry - frozen section; rat immunohistochemistry; rat	RamÃrez Sandoval R, LuÃ©vano RodrÃguez N, RodrÃguez RodrÃguez M, PÃ©rez PÃ©rez M, SaldÃvar Elias S, Gurrola Carlos R, et al. An Animal Model Using Metallic Ions to Produce Autoimmune Nephritis. J Immunol Res. 2015;2015:269610 pubmed publisher
immunoprecipitation; mouse; 1:1000	Chen Y, Chang J, Jong Y, Liu T, Yuo C. High expression level of Tra2-Î²1 is responsible for increased SMN2 exon 7 inclusion in the testis of SMA mice. PLoS ONE. 2015;10:e0120721 pubmed publisher
immunohistochemistry - paraffin section; human; fig 6 immunoprecipitation; human immunocytochemistry; human; fig 4 immunocytochemistry; mouse; fig 1	Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher
immunocytochemistry; roundworm ; 1:250	Rouhana L, Vieira A, Roberts Galbraith R, Newmark P. PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells. Development. 2012;139:1083-94 pubmed publisher
immunoprecipitation; mouse	Jia Y, Mu J, Ackerman S. Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration. Cell. 2012;148:296-308 pubmed publisher
immunoprecipitation; mouse	Shababi M, Glascock J, Lorson C. Combination of SMN trans-splicing and a neurotrophic factor increases the life span and body mass in a severe model of spinal muscular atrophy. Hum Gene Ther. 2011;22:135-44 pubmed publisher
immunocytochemistry; mouse	Fulka H, Novakova Z, Mosko T, Fulka J. The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei. Histochem Cell Biol. 2009;132:457-68 pubmed publisher
immunoprecipitation; human	Kataoka N, Fujita M, Ohno M. Functional association of the Microprocessor complex with the spliceosome. Mol Cell Biol. 2009;29:3243-54 pubmed publisher
immunoprecipitation; human	Coady T, Shababi M, Tullis G, Lorson C. Restoration of SMN function: delivery of a trans-splicing RNA re-directs SMN2 pre-mRNA splicing. Mol Ther. 2007;15:1471-8 pubmed
immunohistochemistry; human; 1:100	Comitato A, Spampanato C, Chakarova C, Sanges D, Bhattacharya S, Marigo V. Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells. Hum Mol Genet. 2007;16:1699-707 pubmed
immunocytochemistry; human; 1:1000 western blot; human	Almstead L, Sarnow P. Inhibition of U snRNP assembly by a virus-encoded proteinase. Genes Dev. 2007;21:1086-97 pubmed
immunocytochemistry; Pacific oyster	Spada F, Koch J, Sadoni N, Mitchell N, Ganot P, De Boni U, et al. Conserved patterns of nuclear compartmentalization are not observed in the chordate Oikopleura. Biol Cell. 2007;99:273-87 pubmed
immunocytochemistry; fruit fly	Anne J, Ollo R, Ephrussi A, Mechler B. Arginine methyltransferase Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation in Drosophila. Development. 2007;134:137-46 pubmed
immunohistochemistry; hamsters	Cameron R, Liu C, Mixon A, Pihkala J, Rahn R, Cameron P. Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression. Cell Motil Cytoskeleton. 2007;64:19-48 pubmed
western blot; human; loading ...; fig 3	Carissimi C, Saieva L, Gabanella F, Pellizzoni L. Gemin8 is required for the architecture and function of the survival motor neuron complex. J Biol Chem. 2006;281:37009-16 pubmed
immunoprecipitation; human	Carissimi C, Saieva L, Baccon J, Chiarella P, Maiolica A, Sawyer A, et al. Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly. J Biol Chem. 2006;281:8126-34 pubmed
immunohistochemistry; mouse	Dehghani H, Reith C, Hahnel A. Subcellular localization of protein kinase C delta and epsilon affects transcriptional and post-transcriptional processes in four-cell mouse embryos. Reproduction. 2005;130:453-65 pubmed
immunocytochemistry; human	Xu H, Somers Z, Robinson M, Hebert M. Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis. BMC Cell Biol. 2005;6:29 pubmed
immunocytochemistry; mouse western blot; mouse	Krauss S, Lo A, Short S, Koury M, Mohandas N, Chasis J. Nuclear substructure reorganization during late-stage erythropoiesis is selective and does not involve caspase cleavage of major nuclear substructural proteins. Blood. 2005;106:2200-5 pubmed
	Ajiro M, Awaya T, Kim Y, Iida K, Denawa M, Tanaka N, et al. Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia. Nat Commun. 2021;12:4507 pubmed publisher
	Norppa A, Frilander M. The integrity of the U12 snRNA 3' stem-loop is necessary for its overall stability. Nucleic Acids Res. 2021;49:2835-2847 pubmed publisher
	Hsieh Y, Guo C, Yalamanchili H, Abreha M, Al Ouran R, Li Y, et al. Tau-Mediated Disruption of the Spliceosome Triggers Cryptic RNA Splicing and Neurodegeneration in Alzheimer's Disease. Cell Rep. 2019;29:301-316.e10 pubmed publisher
	Gao G, Zhang L, Villarreal O, He W, Su D, Bedford E, et al. PRMT1 loss sensitizes cells to PRMT5 inhibition. Nucleic Acids Res. 2019;47:5038-5048 pubmed publisher
	Buskin A, Zhu L, Chichagova V, Basu B, Mozaffari Jovin S, Dolan D, et al. Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nat Commun. 2018;9:4234 pubmed publisher
	King R, Newmark P. In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea. BMC Dev Biol. 2013;13:8 pubmed publisher
	Narayanan U, Achsel T, Luhrmann R, Matera A. Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein. Mol Cell. 2004;16:223-34 pubmed
	Metz A, Soret J, Vourc h C, Tazi J, Jolly C. A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules. J Cell Sci. 2004;117:4551-8 pubmed
	Polak P, Simone F, Kaberlein J, Luo R, Thirman M. ELL and EAF1 are Cajal body components that are disrupted in MLL-ELL leukemia. Mol Biol Cell. 2003;14:1517-28 pubmed
	Pu W, Krapivinsky G, Krapivinsky L, Clapham D. pICln inhibits snRNP biogenesis by binding core spliceosomal proteins. Mol Cell Biol. 1999;19:4113-20 pubmed

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product information

Product Type :

Antibody

Product Name :

SNRPB Monoclonal Antibody (Y12)

Catalog # :

MA5-13449

Quantity :

500 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Drosophila, Human, Mouse, Porcine, Rabbit, Rat

Applications :

Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:100, Immunomicroscopy: Assay-dependent, RNA Immunoprecipitation: 1.5 µg/10^6 cells, Western Blot: 0.5-1.0 µg/mL

Species :

Drosophila, Human, Mouse, Porcine, Rabbit, Rat

Clone :

Y12

Isotype :

IgG3, kappa

Storage :

4° C

Description :

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1:50-1:100, Immunomicroscopy: Assay-dependent, RNA Immunoprecipitation: 1.5 µg/10^6 cells, Western Blot: 0.5-1.0 µg/mL

Aliases :

AL024368; AU018828; B polypeptide of Sm protein; CCMS; COD; sm protein B; Sm protein B/B'; SM11; small nuclear ribonucleoprotein B; small nuclear ribonucleoprotein polypeptide B; small nuclear ribonucleoprotein polypeptides B and B'; small nuclear ribonucleoprotein polypeptides B and B1; small nuclear ribonucleoprotein-associated protein B; Small nuclear ribonucleoprotein-associated proteins B and B'; smB; sm-B; Sm-B/B'; SmB/B'; sm-B/Sm-B'; SmB/SmB'; SNRNP-B; Snrpb; SNRPB1

more info or order :

Invitrogen product webpage