XRCC1 Monoclonal Antibody (33-2-5), Biotin | Invitrogen MA5-13409 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

XRCC1 Monoclonal Antibody (33-2-5), Biotin

catalog :

MA5-13409

quantity :

500 µL

price :

US 456.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

33-2-5

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; human	Masaoka A, Gassman N, Horton J, Kedar P, Witt K, Hobbs C, et al. Interaction between DNA Polymerase ? and BRCA1. PLoS ONE. 2013;8:e66801 pubmed publisher
immunohistochemistry; human; 1:200 western blot; human	Sultana R, Abdel Fatah T, Abbotts R, Hawkes C, Albarakati N, Seedhouse C, et al. Targeting XRCC1 deficiency in breast cancer for personalized therapy. Cancer Res. 2013;73:1621-34 pubmed publisher
immunohistochemistry; human; 1:200	Abdel Fatah T, Sultana R, Abbotts R, Hawkes C, Seedhouse C, Chan S, et al. Clinicopathological and functional significance of XRCC1 expression in ovarian cancer. Int J Cancer. 2013;132:2778-86 pubmed publisher
western blot; human; 1:1000	Perego P, Cossa G, Tinelli S, Corna E, Carenini N, Gatti L, et al. Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition. Biochem Pharmacol. 2012;83:27-36 pubmed publisher
immunocytochemistry; human western blot; human; 1:1000	Jin R, Sun Y, Qi X, Zhang H, Zhang Y, Li N, et al. E2F1 is involved in DNA single-strand break repair through cell-cycle-dependent upregulation of XRCC1 expression. DNA Repair (Amst). 2011;10:926-33 pubmed publisher
western blot; human; 1:1000	Todorova M, Larroque A, Dauphin Pierre S, Fang Y, Jean Claude B. Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species. Mol Cancer Ther. 2010;9:869-82 pubmed publisher
immunohistochemistry; human; 1:300	Kang C, Jang B, Kim D, Chung D, Kim Y, Jheon S, et al. The prognostic significance of ERCC1, BRCA1, XRCC1, and betaIII-tubulin expression in patients with non-small cell lung cancer treated by platinum- and taxane-based neoadjuvant chemotherapy and surgical resection. Lung Cancer. 2010;68:478-83 pubmed publisher
western blot; human	Chou W, Wang H, Wong F, Ding S, Wu P, Shieh S, et al. Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair. EMBO J. 2008;27:3140-50 pubmed publisher
immunocytochemistry; human	Kulkarni A, McNeill D, Gleichmann M, Mattson M, Wilson D. XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells. Nucleic Acids Res. 2008;36:5111-21 pubmed publisher
western blot; human	Kuo C, Liu J, Shiah H, Ma L, Chang J. Tamoxifen accelerates proteasomal degradation of O6-methylguanine DNA methyltransferase in human cancer cells. Int J Cancer. 2007;121:2293-300 pubmed
western blot; human	Bekker Jensen S, Fugger K, Danielsen J, Gromova I, Sehested M, Celis J, et al. Human Xip1 (C2orf13) is a novel regulator of cellular responses to DNA strand breaks. J Biol Chem. 2007;282:19638-43 pubmed
immunocytochemistry; human	Nakajima S, Lan L, Kanno S, Usami N, Kobayashi K, Mori M, et al. Replication-dependent and -independent responses of RAD18 to DNA damage in human cells. J Biol Chem. 2006;281:34687-95 pubmed
western blot; human; 1:150	Bu D, Tomlinson G, Lewis C, Zhang C, Kildebeck E, Euhus D. An intronic polymorphism associated with increased XRCC1 expression, reduced apoptosis and familial breast cancer. Breast Cancer Res Treat. 2006;99:257-65 pubmed
western blot; human	Wong H, Wilson D. XRCC1 and DNA polymerase beta interaction contributes to cellular alkylating-agent resistance and single-strand break repair. J Cell Biochem. 2005;95:794-804 pubmed
immunocytochemistry; human; 1:500 western blot; human; 1:500	Takanami T, Nakamura J, Kubota Y, Horiuchi S. The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability. Mutat Res. 2005;582:135-45 pubmed
	Audebert M, Salles B, Calsou P. Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining. J Biol Chem. 2004;279:55117-26 pubmed
	Lan L, Nakajima S, Oohata Y, Takao M, Okano S, Masutani M, et al. In situ analysis of repair processes for oxidative DNA damage in mammalian cells. Proc Natl Acad Sci U S A. 2004;101:13738-43 pubmed
	Park S, Lam W, Cheng Y. X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance. Cancer Res. 2002;62:459-65 pubmed
	Martinet W, Knaapen M, De Meyer G, Herman A, Kockx M. Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering. Circ Res. 2001;88:733-9 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.

image 2 :

Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.

product information

Product Type :

Antibody

Product Name :

XRCC1 Monoclonal Antibody (33-2-5), Biotin

Catalog # :

MA5-13409

Quantity :

500 µL

Price :

US 456.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Rat

Applications :

Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Rat

Clone :

33-2-5

Isotype :

IgG2b

Storage :

4° C

Description :

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.

Immunogen :

Recombinant human XRCC1 protein

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

DNA repair protein XRCC1; RCC; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross complementing 1; x-ray repair cross-complementing group 1 protein; X-ray repair cross-complementing protein 1; XRCC1; Xrcc-1

more info or order :

Invitrogen product webpage