DNA-PK Monoclonal Antibody (18-2 + 25-4 + 42-psc) | Invitrogen MA5-13404 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DNA-PK Monoclonal Antibody (18-2 + 25-4 + 42-psc)

catalog :

MA5-13404

quantity :

500µL

price :

US 349.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

18-2 + 25-4 + 42-psc

reactivity :

Chinese hamster, human, mouse, rat, chicken, pig, platanna

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments, RNA immunoprecipitation

citations: 90

Published Application/Species/Dilution	Reference
western blot (knockdown validation); human; fig 4 RNA immunoprecipitation; human; fig 3	Zhang Y, He Q, Hu Z, Feng Y, Fan L, Tang Z, et al. Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nat Struct Mol Biol. 2016;23:522-30 pubmed publisher
western blot (knockdown validation); human; fig 3d	Bouley J, Saad L, Grall R, Schellenbauer A, Biard D, Paget V, et al. A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines. Oncotarget. 2015;6:27980-8000 pubmed publisher
western blot; human; fig 6a	Capitanio J, Montpetit B, Wozniak R. Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9. elife. 2017;6: pubmed publisher
western blot; human; 1:1000; fig s2	Bot C, Pfeiffer A, Giordano F, Manjeera D, Dantuma N, Ström L. Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage.. J Cell Sci. 2017;130:1134-1146 pubmed publisher
immunoprecipitation; human; 1:2000; fig 7	He S, Zhao Z, Yang Y, O'Connell D, Zhang X, Oh S, et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015;6:7839 pubmed publisher
western blot; human; fig 5	Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, et al. The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells. Cancer Med. 2015;4:426-36 pubmed publisher
western blot; mouse; 1:500	Gapud E, Dorsett Y, Yin B, Callen E, Bredemeyer A, Mahowald G, et al. Ataxia telangiectasia mutated (Atm) and DNA-PKcs kinases have overlapping activities during chromosomal signal joint formation. Proc Natl Acad Sci U S A. 2011;108:2022-7 pubmed publisher
western blot; human; fig 2	Sakasai R, Teraoka H, Tibbetts R. Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin. DNA Repair (Amst). 2010;9:76-82 pubmed publisher
western blot; mouse; fig 2a	Cousin W, Ho M, Desai R, Tham A, Chen R, Kung S, et al. Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage. PLoS ONE. 2013;8:e63528 pubmed publisher
immunocytochemistry; human; fig 5	Sakasai R, Teraoka H, Takagi M, Tibbetts R. Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison. J Biol Chem. 2010;285:15201-8 pubmed publisher
	Lin Y, Shih H, Shang Z, Matsunaga S, Chen B. DNA-PKcs is required to maintain stability of Chk1 and Claspin for optimal replication stress response. Nucleic Acids Res. 2014;42:4463-73 pubmed publisher
	Nishida Y, Mizutani N, Inoue M, Omori Y, Tamiya-Koizumi K, Takagi A, et al. Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines. Biochim Biophys Acta. 2014;1839:265-74 pubmed publisher
	Mizutani T, Ju Y, Imamichi Y, Osaki T, Yazawa T, Kawabe S, et al. C/EBPβ (CCAAT/enhancer-binding protein β) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1). Biochem J. 2014;460:459-71 pubmed publisher
	Papeta N, Zheng Z, Schon E, Brosel S, Altintas M, Nasr S, et al. Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice. J Clin Invest. 2010;120:4055-64 pubmed publisher
	Schild-Poulter C, Su A, Shih A, Kelly O, Fritzler M, Goldstein R, et al. Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases. Rheumatology (Oxford). 2008;47:165-71 pubmed publisher
	Johns T, McKay M, Cvrljevic A, Gan H, Taylor C, Xu H, et al. MAb 806 enhances the efficacy of ionizing radiation in glioma xenografts expressing the de2-7 epidermal growth factor receptor. Int J Radiat Oncol Biol Phys. 2010;78:572-8 pubmed publisher
	Abbaszadeh F, Clingen P, Arlett C, Plowman P, Bourton E, Themis M, et al. A novel splice variant of the DNA-PKcs gene is associated with clinical and cellular radiosensitivity in a patient with xeroderma pigmentosum. J Med Genet. 2010;47:176-81 pubmed publisher
	Velkova A, Carvalho M, Johnson J, Tavtigian S, Monteiro A. Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle. 2010;9:1421-33 pubmed
	Gao P, Yoo S, Lee K, Rosensweig C, Takahashi J, Chen B, et al. Phosphorylation of the cryptochrome 1 C-terminal tail regulates circadian period length. J Biol Chem. 2013;288:35277-86 pubmed publisher
	Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res. 2011;39:9605-19 pubmed publisher
	Giffin W, Gong W, Schild-Poulter C, Hache R. Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription. Mol Cell Biol. 1999;19:4065-78 pubmed
	Meulle A, Salles B, Daviaud D, Valet P, Muller C. Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis. PLoS ONE. 2008;3:e3345 pubmed publisher
	Choi Y, Nash K, Byrne B, Muzyczka N, Song S. The effect of DNA-dependent protein kinase on adeno-associated virus replication. PLoS ONE. 2010;5:e15073 pubmed publisher
	Mo X, Dynan W. Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites. Mol Cell Biol. 2002;22:8088-99 pubmed
	Mandic A, Viktorsson K, Molin M, Eguchi H, Hayashi S, Toi M, et al. Cisplatin induces the proapoptotic conformation of Bak in a deltaMEKK1-dependent manner. Mol Cell Biol. 2001;21:3684-91 pubmed
	Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D. DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression. Cell Death Differ. 2009;16:795-806 pubmed publisher
	Sand-Dejmek J, Adelmant G, Sobhian B, Calkins A, Marto J, Iglehart D, et al. Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin. Mol Cancer. 2011;10:74 pubmed publisher
	Noguchi M, Yu D, Hirayama R, Ninomiya Y, Sekine E, Kubota N, et al. Inhibition of homologous recombination repair in irradiated tumor cells pretreated with Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin. Biochem Biophys Res Commun. 2006;351:658-63 pubmed
	Britton S, Frit P, Biard D, Salles B, Calsou P. ARTEMIS nuclease facilitates apoptotic chromatin cleavage. Cancer Res. 2009;69:8120-6 pubmed publisher
	Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L. Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies. Exp Mol Pathol. 2004;77:1-6 pubmed
	Zumer K, Low A, Jiang H, Saksela K, Peterlin B. Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes. Mol Cell Biol. 2012;32:1354-62 pubmed publisher
	Das A, Chen B, Story M, Sato M, Minna J, Chen D, et al. Somatic mutations in the tyrosine kinase domain of epidermal growth factor receptor (EGFR) abrogate EGFR-mediated radioprotection in non-small cell lung carcinoma. Cancer Res. 2007;67:5267-74 pubmed
	Volcic M, Karl S, Baumann B, Salles D, Daniel P, Fulda S, et al. NF-κB regulates DNA double-strand break repair in conjunction with BRCA1-CtIP complexes. Nucleic Acids Res. 2012;40:181-95 pubmed publisher
	Friesland S, Kanter-Lewensohn L, Tell R, Munck-Wikland E, Lewensohn R, Nilsson A. Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy. Head Neck. 2003;25:313-21 pubmed
	Hu S, Qu Y, Xu X, Xu Q, Geng J, Xu J. Nuclear survivin and its relationship to DNA damage repair genes in non-small cell lung cancer investigated using tissue array. PLoS ONE. 2013;8:e74161 pubmed publisher
	Viana-Pereira M, Lee A, Popov S, Bax D, Al-Sarraj S, Bridges L, et al. Microsatellite instability in pediatric high grade glioma is associated with genomic profile and differential target gene inactivation. PLoS ONE. 2011;6:e20588 pubmed publisher
	Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Gueret M. Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing. Nucleic Acids Res. 2003;31:6272-82 pubmed
	Shackelford D. DNA end joining activity is reduced in Alzheimer's disease. Neurobiol Aging. 2006;27:596-605 pubmed
	Ohno M, Kunimoto M, Nishizuka M, Osada S, Imagawa M. Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression. Biochem Biophys Res Commun. 2009;390:738-42 pubmed publisher
	Udayakumar D, Bladen C, Hudson F, Dynan W. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003;278:41631-5 pubmed
	Wang H, Perrault A, Takeda Y, Qin W, Wang H, Iliakis G. Biochemical evidence for Ku-independent backup pathways of NHEJ. Nucleic Acids Res. 2003;31:5377-88 pubmed
	Luo B, Yu S, Zhuang L, Xia S, Zhao Z, Rong L. Induction of ERBB2 nuclear transport after radiation in breast cancer cells. J Huazhong Univ Sci Technolog Med Sci. 2009;29:350-3 pubmed publisher
	Dean J, McClendon A, Knudsen E. Modification of the DNA damage response by therapeutic CDK4/6 inhibition. J Biol Chem. 2012;287:29075-87 pubmed publisher
	Deutsch E, Maggiorella L, Wen B, Bonnet M, Khanfir K, Frascogna V, et al. Tyrosine kinase inhibitor AG1024 exerts antileukaemic effects on STI571-resistant Bcr-Abl expressing cells and decreases AKT phosphorylation. Br J Cancer. 2004;91:1735-41 pubmed
	Landais I, Lee H, Lu H. Coupling caspase cleavage and ubiquitin-proteasome-dependent degradation of SSRP1 during apoptosis. Cell Death Differ. 2006;13:1866-78 pubmed
	Croset A, Cordelières F, Berthault N, Buhler C, Sun J, Quanz M, et al. Inhibition of DNA damage repair by artificial activation of PARP with siDNA. Nucleic Acids Res. 2013;41:7344-55 pubmed publisher
	Ruiz-Herrera A, Smirnova A, Khouriauli L, Nergadze S, Mondello C, Giulotto E. Gene amplification in human cells knocked down for RAD54. Genome Integr. 2011;2:5 pubmed publisher
	Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay J. The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination. J Exp Med. 2004;199:315-21 pubmed
	Mastrocola A, Kim S, Trinh A, Rodenkirch L, Tibbetts R. The RNA-binding protein fused in sarcoma (FUS) functions downstream of poly(ADP-ribose) polymerase (PARP) in response to DNA damage. J Biol Chem. 2013;288:24731-41 pubmed publisher
	M'kacher R, Bennaceur A, Farace F, Lauge A, Plassa L, Wittmer E, et al. Multiple molecular mechanisms contribute to radiation sensitivity in mantle cell lymphoma. Oncogene. 2003;22:7905-12 pubmed
	Takai H, Xie Y, de Lange T, Pavletich N. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev. 2010;24:2019-30 pubmed publisher
	Shimomura A, Takasaki A, Nomura R, Hayashi N, Senda T. Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1. Med Mol Morphol. 2013;46:14-9 pubmed publisher
	Shao R, Cao C, Zhang H, Kohn K, Wold M, Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999;18:1397-406 pubmed
	Iwabuchi K, Matsui T, Hashimoto M, Matsumoto Y, Kurihara T, Date T. Characterization of a cancer cell line that expresses a splicing variant form of 53BP1: separation of checkpoint and repair functions in 53BP1. Biochem Biophys Res Commun. 2008;376:509-13 pubmed publisher
	Zhong X, Safa A. Phosphorylation of RNA helicase A by DNA-dependent protein kinase is indispensable for expression of the MDR1 gene product P-glycoprotein in multidrug-resistant human leukemia cells. Biochemistry. 2007;46:5766-75 pubmed
	Choul-Li S, Drobecq H, Aumercier M. DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms. Biochem Biophys Res Commun. 2009;390:839-44 pubmed publisher
	Böhringer M, Obermeier K, Griner N, Waldraff D, Dickinson E, Eirich K, et al. siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors. Oncogene. 2013;32:5458-70 pubmed publisher
	Kang M, Jung S, Kim M, Bae J, Kim H, Kim J, et al. DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells. FEBS J. 2008;275:5969-81 pubmed publisher
	Lau A, Swinbank K, Ahmed P, Taylor D, Jackson S, Smith G, et al. Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase. Nat Cell Biol. 2005;7:493-500 pubmed
	Dregalla R, Zhou J, Idate R, Battaglia C, Liber H, Bailey S. Regulatory roles of tankyrase 1 at telomeres and in DNA repair: suppression of T-SCE and stabilization of DNA-PKcs. Aging (Albany NY). 2010;2:691-708 pubmed
	Javvadi P, Makino H, Das A, Lin Y, Chen D, Chen B, et al. Threonine 2609 phosphorylation of the DNA-dependent protein kinase is a critical prerequisite for epidermal growth factor receptor-mediated radiation resistance. Mol Cancer Res. 2012;10:1359-68 pubmed publisher
	Brown K, Lataxes T, Shangary S, Mannino J, Giardina J, Chen J, et al. Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism. J Biol Chem. 2000;275:6651-6 pubmed
	Wu P, Frit P, Malivert L, Revy P, Biard D, Salles B, et al. Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell. J Biol Chem. 2007;282:31937-43 pubmed
	Yasaei H, Slijepcevic P. Defective Artemis causes mild telomere dysfunction. Genome Integr. 2010;1:3 pubmed publisher
	Cho N, Cordon-Cardo C, Li G, Kim S. Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults. J Pathol. 2002;198:21-9 pubmed
	Ma Y, Pannicke U, Schwarz K, Lieber M. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002;108:781-94 pubmed
	Chen L, Trujillo K, Sung P, Tomkinson A. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase. J Biol Chem. 2000;275:26196-205 pubmed
	Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, De Stefano M, et al. Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity. J Biol Chem. 2012;287:2618-31 pubmed publisher
	Song S, Lu Y, Choi Y, Han Y, Tang Q, Zhao G, et al. DNA-dependent PK inhibits adeno-associated virus DNA integration. Proc Natl Acad Sci U S A. 2004;101:2112-6 pubmed
	Lebrasseur N, Cote G, Miller T, Fielding R, Sawyer D. Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle. Am J Physiol Cell Physiol. 2003;284:C1149-55 pubmed
	Calsou P, Delteil C, Frit P, Drouet J, Salles B. Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment. J Mol Biol. 2003;326:93-103 pubmed
	Daya S, Cortez N, Berns K. Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway. J Virol. 2009;83:11655-64 pubmed publisher
	Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, et al. A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia. J Cell Sci. 2011;124:1943-51 pubmed publisher
	Sekine-Suzuki E, Yu D, Kubota N, Okayasu R, Anzai K. Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells. Biochem Biophys Res Commun. 2008;377:341-5 pubmed publisher
	Sawchuk D, Mansilla-Soto J, Alarcon C, Singha N, Langen H, Bianchi M, et al. Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule. J Biol Chem. 2004;279:29821-31 pubmed
	Hsu H, Gilley D, Blackburn E, Chen D. Ku is associated with the telomere in mammals. Proc Natl Acad Sci U S A. 1999;96:12454-8 pubmed
	Perrault R, Wang H, Wang M, Rosidi B, Iliakis G. Backup pathways of NHEJ are suppressed by DNA-PK. J Cell Biochem. 2004;92:781-94 pubmed
	Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R. Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo. Cancer Sci. 2008;99:810-5 pubmed publisher
	Yu X, Wang H, Wang P, Chen B, Wang Y. The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival. J Cell Physiol. 2011;226:369-74 pubmed publisher
	Hegarat N, Cardoso G, Rusconi F, Francois J, Praseuth D. Analytical biochemistry of DNA--protein assemblies from crude cell extracts. Nucleic Acids Res. 2007;35:e92 pubmed
	Calkins A, Iglehart J, Lazaro J. DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1. Nucleic Acids Res. 2013;41:7378-86 pubmed publisher
	Jiang X, Sun Y, Chen S, Roy K, Price B. The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM. J Biol Chem. 2006;281:15741-6 pubmed
	Mauro M, Rego M, Boisvert R, Esashi F, Cavallo F, Jasin M, et al. p21 promotes error-free replication-coupled DNA double-strand break repair. Nucleic Acids Res. 2012;40:8348-60 pubmed
	Kang K, Zhu C, Wong Y, Gao Q, Ty A, Wong M. Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair. Int J Radiat Oncol Biol Phys. 2012;83:e43-52 pubmed publisher
	Maeda J, Yurkon C, Fujisawa H, Kaneko M, Genet S, Roybal E, et al. Genomic instability and telomere fusion of canine osteosarcoma cells. PLoS ONE. 2012;7:e43355 pubmed publisher
	Nguyen C, Possemato R, Bauerlein E, Xie A, Scully R, Hahn W. Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts. Mol Cell Biol. 2012;32:3963-77 pubmed publisher
	Ma C, Muranyi M, Chu C, Zhang J, Chu W. Involvement of DNA-PKcs in the IL-6 and IL-12 response to CpG-ODN is mediated by its interaction with TRAF6 in dendritic cells. PLoS ONE. 2013;8:e58072 pubmed publisher
	Jhappan C, Yusufzai T, Anderson S, Anver M, Merlino G. The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase. Mol Cell Biol. 2000;20:4075-83 pubmed
	Loong S, Korzh S, Price A. Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis. Oncogene. 2004;23:5562-6 pubmed
	Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver D, Alt F. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity. 1998;9:367-76 pubmed

product information

Product Type :

Antibody

Product Name :

DNA-PK Monoclonal Antibody (18-2 + 25-4 + 42-psc)

Catalog # :

MA5-13404

Quantity :

500µL

Price :

US 349.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Chicken, Hamster, Human, Mouse, Xenopus laevis

Applications :

Flow Cytometry: 3-5 µg/10^6 cells, Gel Shift: 1mg/mL, Immunocytochemistry: 2 µg/ml, Immunofluorescence: 2 µg/ml, Immunohistochemistry (Paraffin): 2-4 µg/ml, Immunoprecipitation: 2 µg/mg protein lysate, Western Blot: 1:1000

Species :

Chicken, Hamster, Human, Mouse, Xenopus laevis

Clone :

18-2 + 25-4 + 42-psc

Isotype :

IgG1/IgG2a

Storage :

4° C

Description :

DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks

Immunogen :

Human DNA-dependent Protein Kinase purified from HeLa cells

Format :

Liquid

Applications w/Dilutions :

Aliases :

DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, hyper-radiosensitivity of murine scid mutation, complementing 1, p460

PubMed References :

10064605, 10330147, 10535943, 10692474, 10805749, 10854421, 11340162, 11955432, 12210059, 12391174, 12519750, 12547193, 12658736, 12917393, 12954774, 12970738, 14576316, 14744996, 14766968, 15123719, 15184871, 15211575, 15215044, 15494718, 15834407, 15908050, 16498457, 16603769, 17083915, 17441731, 17545606, 17617645, 17720816, 18208821, 18377429, 18804090, 18846213, 18854174, 19021771, 19229246, 19513620, 19759155, 19797196, 19808974, 19833092, 19836356, 19959400, 20304914, 20305393, 20638193, 20665702, 20678254, 20801936, 20978358, 21037379, 21188139, 21245316, 21418575, 21576354, 21637783, 21880593, 21908405, 22139836, 22310661, 22516386, 22733811, 22735704, 22851694, 22916246, 23325550, 23435420, 23533581, 23704914, 23761435, 23775790, 23833192, 24066112, 24158435, 24500207, 24530422, 24635384, 25582113, 26234763, 26337656, 27111890, 9768756