product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Thrombospondin 1 Monoclonal Antibody (A6.1)
catalog :
MA5-13398
quantity :
200 µL
price :
US 425.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A6.1
reactivity :
human, mouse, rat, dogs, bovine, pigs , domestic sheep, domestic horse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 149
Published Application/Species/Sample/DilutionReference
  • western blot; rat; loading ...; fig 9a
Dunford E, Leclair E, Aiken J, Mandel E, Haas T, Birot O, et al. The effects of voluntary exercise and prazosin on capillary rarefaction and metabolism in streptozotocin-induced diabetic male rats. J Appl Physiol (1985). 2017;122:492-502 pubmed publisher
  • western blot; rat; loading ...; fig 3f
Mandel E, Dunford E, Trifonova A, Abdifarkosh G, Teich T, Riddell M, et al. Prazosin Can Prevent Glucocorticoid Mediated Capillary Rarefaction. PLoS ONE. 2016;11:e0166899 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; fig 4
Kim S, Hayashi H, Ishikawa T, Shibata K, Shigetomi E, Shinozaki Y, et al. Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain. J Clin Invest. 2016;126:1983-97 pubmed publisher
  • western blot; human; fig 5
Posner M, Upadhyay A, Abubaker A, Fortunato T, Vara D, Canobbio I, et al. Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes. J Biol Chem. 2016;291:2764-76 pubmed publisher
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 1
Labrousse Arias D, Castillo González R, Rogers N, Torres Capelli M, Barreira B, Aragonés J, et al. HIF-2α-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction. Cardiovasc Res. 2016;109:115-30 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 2
Kuroki H, Hayashi H, Nakagawa S, Sakamoto K, Higashi T, Nitta H, et al. Effect of LSKL peptide on thrombospondin 1-mediated transforming growth factor β signal activation and liver regeneration after hepatectomy in an experimental model. Br J Surg. 2015;102:813-25 pubmed publisher
  • western blot; human; fig 3
de la Rica L, García Gómez A, Comet N, Rodríguez Ubreva J, Ciudad L, Vento Tormo R, et al. NF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol. 2015;16:2 pubmed publisher
  • western blot; mouse
Fei P, Zaitoun I, Farnoodian M, Fisk D, Wang S, Sorenson C, et al. Expression of thrombospondin-1 modulates the angioinflammatory phenotype of choroidal endothelial cells. PLoS ONE. 2014;9:e116423 pubmed publisher
  • western blot; mouse
Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N. High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS ONE. 2014;9:e103148 pubmed publisher
  • western blot; human
Talme T, Bergdahl E, Sundqvist K. Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1. Immunology. 2014;142:176-92 pubmed publisher
  • immunohistochemistry; rat
Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C. Long-term gene therapy with thrombospondin 2 inhibits TGF-? activation, inflammation and angiogenesis in chronic allograft nephropathy. PLoS ONE. 2013;8:e83846 pubmed publisher
  • western blot; mouse
Shin E, Sorenson C, Sheibani N. PEDF expression regulates the proangiogenic and proinflammatory phenotype of the lung endothelium. Am J Physiol Lung Cell Mol Physiol. 2014;306:L620-34 pubmed publisher
  • western blot; mouse
Morrison M, Palenski T, Jamali N, Sheibani N, Sorenson C. Modulation of vascular cell function by bim expression. Int J Cell Biol. 2013;2013:297537 pubmed publisher
  • western blot; mouse; 1:1,000
Zhao Y, Wang S, Sorenson C, Teixeira L, Dubielzig R, Peters D, et al. Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress. Mol Cell Biol. 2013;33:4225-40 pubmed publisher
  • western blot; human
Bergström S, Bergdahl E, Sundqvist K. A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation. Immunology. 2013;140:441-55 pubmed publisher
  • western blot; mouse
Palenski T, Sorenson C, Sheibani N. Inflammatory cytokine-specific alterations in retinal endothelial cell function. Microvasc Res. 2013;89:57-69 pubmed publisher
  • western blot; mouse
Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-? signaling pathways. J Cell Sci. 2013;126:1392-405 pubmed publisher
  • immunohistochemistry; human; 1:50
Bhattacharyya S, Sul K, Krukovets I, Nestor C, Li J, Adognravi O. Novel tissue-specific mechanism of regulation of angiogenesis and cancer growth in response to hyperglycemia. J Am Heart Assoc. 2012;1:e005967 pubmed publisher
  • ELISA; human; 1:500
Martin Manso G, Navarathna D, Galli S, Soto Pantoja D, Kuznetsova S, Tsokos M, et al. Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis. PLoS ONE. 2012;7:e48775 pubmed publisher
  • western blot; mouse; fig 4
Grutzmacher C, Park S, Zhao Y, Morrison M, Sheibani N, Sorenson C. Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes. Am J Physiol Renal Physiol. 2013;304:F19-30 pubmed publisher
  • immunohistochemistry; human; 1:100
Alkim C, Sakiz D, Alkim H, Livaoglu A, Kendir T, Demirsoy H, et al. Thrombospondin-1 and VEGF in inflammatory bowel disease. Libyan J Med. 2012;7: pubmed publisher
  • western blot; rat
Kessler Icekson G, Hochhauser E, Sinai T, Kremer A, Dick J, Tarasenko N, et al. A histone deacetylase inhibitory prodrug - butyroyloxymethyl diethyl phosphate - protects the heart and cardiomyocytes against ischemia injury. Eur J Pharm Sci. 2012;45:592-9 pubmed publisher
  • western blot; mouse
Sheibani N, Morrison M, Gurel Z, Park S, Sorenson C. BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment. Am J Physiol Renal Physiol. 2012;302:F809-19 pubmed publisher
  • immunohistochemistry; rat
Matsuba M, Hutcheon A, Zieske J. Localization of thrombospondin-1 and myofibroblasts during corneal wound repair. Exp Eye Res. 2011;93:534-40 pubmed publisher
  • western blot; human; 1:400
El Btaouri H, Morjani H, Greffe Y, Charpentier E, Martiny L. Role of JNK/ATF-2 pathway in inhibition of thrombospondin-1 (TSP-1) expression and apoptosis mediated by doxorubicin and camptothecin in FTC-133 cells. Biochim Biophys Acta. 2011;1813:695-703 pubmed publisher
  • western blot; human; 1:500
Patten S, Adamcic U, Lacombe K, Minhas K, Skowronski K, Coomber B. VEGFR2 heterogeneity and response to anti-angiogenic low dose metronomic cyclophosphamide treatment. BMC Cancer. 2010;10:683 pubmed publisher
  • western blot; human
Dews M, Fox J, Hultine S, Sundaram P, Wang W, Liu Y, et al. The myc-miR-17~92 axis blunts TGF{beta} signaling and production of multiple TGF{beta}-dependent antiangiogenic factors. Cancer Res. 2010;70:8233-46 pubmed publisher
  • western blot; rat
Ikeda H, Miyatake M, Koshikawa N, Ochiai K, Yamada K, Kiss A, et al. Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation. J Biol Chem. 2010;285:38415-27 pubmed publisher
  • immunohistochemistry; mouse
Wang Y, Cheng W, Ke Y, Cai Z, Chen L, Xi Y, et al. Effect of Qingre Quyu Granule (??????) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice. Chin J Integr Med. 2010;16:442-7 pubmed publisher
  • western blot; rat
Tarasenko N, Kessler Icekson G, Boer P, Inbal A, Schlesinger H, Phillips D, et al. The histone deacetylase inhibitor butyroyloxymethyl diethylphosphate (AN-7) protects normal cells against toxicity of anticancer agents while augmenting their anticancer activity. Invest New Drugs. 2012;30:130-43 pubmed publisher
  • western blot; mouse; 1:1000
Park S, Dimaio T, Scheef E, Sorenson C, Sheibani N. PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions. Am J Physiol Cell Physiol. 2010;299:C1468-84 pubmed publisher
  • immunohistochemistry; human; 1:50
Guerrero D, Guarch R, Ojer A, Casas J, Méndez Meca C, Esteller M, et al. Differential hypermethylation of genes in vulvar cancer and lichen sclerosus coexisting or not with vulvar cancer. Int J Cancer. 2011;128:2853-64 pubmed publisher
  • western blot; human; 1:250
Hohberg M, Knöchel J, Hoffmann C, Chlench S, Wunderlich W, Alter A, et al. Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries. J Cell Physiol. 2011;226:350-61 pubmed publisher
  • western blot; mouse
Grutzmacher C, Park S, Elmergreen T, Tang Y, Scheef E, Sheibani N, et al. Opposing effects of bim and bcl-2 on lung endothelial cell migration. Am J Physiol Lung Cell Mol Physiol. 2010;299:L607-20 pubmed publisher
  • immunohistochemistry; mouse
Lanz T, Ding Z, Ho P, Luo J, Agrawal A, Srinagesh H, et al. Angiotensin II sustains brain inflammation in mice via TGF-beta. J Clin Invest. 2010;120:2782-94 pubmed publisher
  • western blot; human
Tan K, Lessieur E, Cutler A, Nerone P, Vasanji A, Asosingh K, et al. Impaired function of circulating CD34(+) CD45(-) cells in patients with proliferative diabetic retinopathy. Exp Eye Res. 2010;91:229-37 pubmed publisher
  • western blot; human
Olsson A, Björk A, Vallon Christersson J, Isaacs J, Leanderson T. Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors. Mol Cancer. 2010;9:107 pubmed publisher
  • immunohistochemistry; human
  • western blot; human
Haldar S, Lu Y, Jeyaraj D, Kawanami D, Cui Y, Eapen S, et al. Klf15 deficiency is a molecular link between heart failure and aortic aneurysm formation. Sci Transl Med. 2010;2:26ra26 pubmed publisher
  • immunocytochemistry; mouse; 1:70
  • immunohistochemistry; mouse; 1:70
  • western blot; mouse; 1:200
Yonezawa T, Hattori S, Inagaki J, Kurosaki M, Takigawa T, Hirohata S, et al. Type IV collagen induces expression of thrombospondin-1 that is mediated by integrin alpha1beta1 in astrocytes. Glia. 2010;58:755-67 pubmed publisher
  • immunohistochemistry; human; 1:500
Zaslavsky A, Baek K, Lynch R, Short S, Grillo J, Folkman J, et al. Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis. Blood. 2010;115:4605-13 pubmed publisher
  • immunoprecipitation; mouse; 2 ug/time
  • immunohistochemistry; mouse; 1:50
  • western blot; mouse
Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls J, et al. The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1. J Biol Chem. 2010;285:6770-80 pubmed publisher
  • immunohistochemistry; rat
Daniel C, Wagner A, Hohenstein B, Hugo C. Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation. Am J Physiol Renal Physiol. 2009;297:F1299-309 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Park C, Kim H, Lim J, Yoo K, Chung S, Shin S, et al. Vascular endothelial growth factor inhibition by dRK6 causes endothelial apoptosis, fibrosis, and inflammation in the heart via the Akt/eNOS axis in db/db mice. Diabetes. 2009;58:2666-76 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Rex S, Beaulieu L, Perlman D, Vitseva O, Blair P, McComb M, et al. Immune versus thrombotic stimulation of platelets differentially regulates signalling pathways, intracellular protein-protein interactions, and alpha-granule release. Thromb Haemost. 2009;102:97-110 pubmed publisher
  • immunohistochemistry; rat
Szasz T, Eddy S, Paulauskis J, Burnett R, Ellekilde M, Iovanna J, et al. Differential expression of pancreatitis-associated protein and thrombospondins in arterial versus venous tissues. J Vasc Res. 2009;46:551-60 pubmed publisher
  • western blot; human; 1:200
Hyder S, Liang Y, Wu J, Welbern V. Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells. Endocr Relat Cancer. 2009;16:809-17 pubmed publisher
  • blocking or activating experiments; mouse
Isenberg J, Shiva S, Gladwin M. Thrombospondin-1-CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO-cGMP pathway activation. Nitric Oxide. 2009;21:52-62 pubmed publisher
  • western blot; human; 1:200
Hyder S, Liang Y, Wu J. Estrogen regulation of thrombospondin-1 in human breast cancer cells. Int J Cancer. 2009;125:1045-53 pubmed publisher
  • western blot; human; 1:400
Scheef E, Sorenson C, Sheibani N. Attenuation of proliferation and migration of retinal pericytes in the absence of thrombospondin-1. Am J Physiol Cell Physiol. 2009;296:C724-34 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Isenberg J, Maxhimer J, Powers P, Tsokos M, Frazier W, Roberts D. Treatment of liver ischemia-reperfusion injury by limiting thrombospondin-1/CD47 signaling. Surgery. 2008;144:752-61 pubmed publisher
  • western blot; mouse
Huang Q, Sheibani N. High glucose promotes retinal endothelial cell migration through activation of Src, PI3K/Akt1/eNOS, and ERKs. Am J Physiol Cell Physiol. 2008;295:C1647-57 pubmed publisher
  • blocking or activating experiments; human
Gayen Betal S, Setty B. Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain. Transl Res. 2008;152:165-77 pubmed publisher
  • blocking or activating experiments; rat; 20 ug/ml
Anderson C, Hastings N, Blackman B, Price R. Capillary sprout endothelial cells exhibit a CD36 low phenotype: regulation by shear stress and vascular endothelial growth factor-induced mechanism for attenuating anti-proliferative thrombospondin-1 signaling. Am J Pathol. 2008;173:1220-8 pubmed publisher
  • immunohistochemistry; mouse; 8 ug/ml
Gruber H, Bornstein P, Sage E, Ingram J, Zinchenko N, Norton H, et al. Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc. Arthritis Res Ther. 2008;10:R96 pubmed publisher
  • western blot; human
Mazan Mamczarz K, Hagner P, Corl S, Srikantan S, Wood W, Becker K, et al. Post-transcriptional gene regulation by HuR promotes a more tumorigenic phenotype. Oncogene. 2008;27:6151-63 pubmed publisher
  • immunohistochemistry; human
Ide M, Ishii H, Mukae H, Iwata A, Sakamoto N, Kadota J, et al. High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia. Respir Med. 2008;102:1625-30 pubmed publisher
  • immunohistochemistry; mouse; 1:400
Johnson A, O Connell M, Miyamoto H, Huang J, Yao J, Messing E, et al. Androgenic dependence of exophytic tumor growth in a transgenic mouse model of bladder cancer: a role for thrombospondin-1. BMC Urol. 2008;8:7 pubmed publisher
  • immunohistochemistry; human; 1:2000
  • western blot; human
Rojas A, Meherem S, Kim Y, Washington M, Willis J, Markowitz S, et al. The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer. Int J Cancer. 2008;123:14-21 pubmed publisher
  • western blot; mouse
Olerud J, Johansson M, Lawler J, Welsh N, Carlsson P. Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets. Diabetes. 2008;57:1870-7 pubmed publisher
  • western blot; mouse
Kondo S, Tang Y, Scheef E, Sheibani N, Sorenson C. Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2. Am J Physiol Cell Physiol. 2008;294:C1521-30 pubmed publisher
  • immunohistochemistry; human; 1:50
Guerrero D, Guarch R, Ojer A, Casas J, Ropero S, Mancha A, et al. Hypermethylation of the thrombospondin-1 gene is associated with poor prognosis in penile squamous cell carcinoma. BJU Int. 2008;102:747-55 pubmed publisher
  • immunoprecipitation; human
Bhattacharyya S, Marinic T, Krukovets I, Hoppe G, Stenina O. Cell type-specific post-transcriptional regulation of production of the potent antiangiogenic and proatherogenic protein thrombospondin-1 by high glucose. J Biol Chem. 2008;283:5699-707 pubmed
  • immunohistochemistry; human
  • western blot; human
Park J, Lee H, Ko K, Park S, Kim J, Choe G, et al. Meteorin regulates angiogenesis at the gliovascular interface. Glia. 2008;56:247-58 pubmed
  • western blot; mouse
Scheef E, Huang Q, Wang S, Sorenson C, Sheibani N. Isolation and characterization of corneal endothelial cells from wild type and thrombospondin-1 deficient mice. Mol Vis. 2007;13:1483-95 pubmed
  • blocking or activating experiments; human
Derks R, Jankowska Gan E, Xu Q, Burlingham W. Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1. J Immunol. 2007;179:3443-51 pubmed
  • blocking or activating experiments; human; 1:1000
  • western blot; human
Hong S, Chang S, Yeom D, Kang J, Hong K. Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A. Anticancer Drugs. 2007;18:1005-14 pubmed
  • immunohistochemistry; domestic sheep; 1:50
Sozo F, Hooper S, Wallace M. Thrombospondin-1 expression and localization in the developing ovine lung. J Physiol. 2007;584:625-35 pubmed
  • ELISA; human
Annis D, Gunderson K, Mosher D. Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations. J Biol Chem. 2007;282:27067-75 pubmed
  • immunohistochemistry; human
Pohlers D, Beyer A, Koczan D, Wilhelm T, Thiesen H, Kinne R. Constitutive upregulation of the transforming growth factor-beta pathway in rheumatoid arthritis synovial fibroblasts. Arthritis Res Ther. 2007;9:R59 pubmed
  • western blot; mouse; 1:3000
Kondo S, Scheef E, Sheibani N, Sorenson C. PECAM-1 isoform-specific regulation of kidney endothelial cell migration and capillary morphogenesis. Am J Physiol Cell Physiol. 2007;292:C2070-83 pubmed
  • immunohistochemistry; rat
de Nigris F, Balestrieri M, Williams Ignarro S, D Armiento F, Fiorito C, Ignarro L, et al. The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats. Nitric Oxide. 2007;17:50-4 pubmed
  • immunohistochemistry; mouse
Kaipainen A, Kieran M, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, et al. PPARalpha deficiency in inflammatory cells suppresses tumor growth. PLoS ONE. 2007;2:e260 pubmed
  • immunohistochemistry; mouse
Isenberg J, Romeo M, Abu Asab M, Tsokos M, Oldenborg A, Pappan L, et al. Increasing survival of ischemic tissue by targeting CD47. Circ Res. 2007;100:712-20 pubmed
  • immunocytochemistry; human; 2 ug/ml
Raman P, Krukovets I, Marinic T, Bornstein P, Stenina O. Glycosylation mediates up-regulation of a potent antiangiogenic and proatherogenic protein, thrombospondin-1, by glucose in vascular smooth muscle cells. J Biol Chem. 2007;282:5704-14 pubmed
  • immunohistochemistry; human
Kopp H, Hooper A, Broekman M, Avecilla S, Petit I, Luo M, et al. Thrombospondins deployed by thrombopoietic cells determine angiogenic switch and extent of revascularization. J Clin Invest. 2006;116:3277-91 pubmed
  • western blot; mouse; 1:4000
Sheibani N, Scheef E, Dimaio T, Wang Y, Kondo S, Sorenson C. Bcl-2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells. J Cell Physiol. 2007;210:616-25 pubmed
  • immunohistochemistry; mouse; 1:25
Isenberg J, Hyodo F, Matsumoto K, Romeo M, Abu Asab M, Tsokos M, et al. Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide-mediated vascular smooth muscle relaxation. Blood. 2007;109:1945-52 pubmed
  • immunohistochemistry; mouse; 1:50
Giuriato S, Ryeom S, Fan A, Bachireddy P, Lynch R, Rioth M, et al. Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch. Proc Natl Acad Sci U S A. 2006;103:16266-71 pubmed
  • immunocytochemistry; human
Kuznetsova S, Issa P, Perruccio E, Zeng B, Sipes J, Ward Y, et al. Versican-thrombospondin-1 binding in vitro and colocalization in microfibrils induced by inflammation on vascular smooth muscle cells. J Cell Sci. 2006;119:4499-509 pubmed
  • immunohistochemistry; human; 1:500
Duvic M, Talpur R, Ni X, Zhang C, Hazarika P, Kelly C, et al. Phase 2 trial of oral vorinostat (suberoylanilide hydroxamic acid, SAHA) for refractory cutaneous T-cell lymphoma (CTCL). Blood. 2007;109:31-9 pubmed
  • immunohistochemistry; mouse; 1:50
Shoji T, Koyama H, Morioka T, Tanaka S, Kizu A, Motoyama K, et al. Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes. Diabetes. 2006;55:2245-55 pubmed
  • immunohistochemistry; human; 4 ug/ml
Wen X, Yang G, Mao W, Thornton A, Liu J, Bast R, et al. HER2 signaling modulates the equilibrium between pro- and antiangiogenic factors via distinct pathways: implications for HER2-targeted antibody therapy. Oncogene. 2006;25:6986-96 pubmed
  • western blot; mouse
Wang Y, Wang S, Sheibani N. Enhanced proangiogenic signaling in thrombospondin-1-deficient retinal endothelial cells. Microvasc Res. 2006;71:143-51 pubmed
  • immunohistochemistry; human; 1:50
Bodner Adler B, Nather A, Bodner K, Czerwenka K, Kimberger O, Leodolter S, et al. Expression of thrombospondin 1 (TSP 1) in patients with uterine smooth muscle tumors: an immunohistochemical study. Gynecol Oncol. 2006;103:186-9 pubmed
  • immunohistochemistry; rat
Johansson M, Mattsson G, Andersson A, Jansson L, Carlsson P. Islet endothelial cells and pancreatic beta-cell proliferation: studies in vitro and during pregnancy in adult rats. Endocrinology. 2006;147:2315-24 pubmed
  • immunohistochemistry; human; 1,000 ug/ml
  • western blot; human; 1:200
Jo W, Mizukami Y, Duerr E, Zukerberg L, Chung D. Wnt signaling can repress thrombospondin-1 expression in colonic tumorigenesis. Cancer Biol Ther. 2005;4:1361-6 pubmed
  • immunohistochemistry; human; 1:50
Sutton C, O Byrne K, Goddard J, Marshall L, Jones L, Garcea G, et al. Expression of thrombospondin-1 in resected colorectal liver metastases predicts poor prognosis. Clin Cancer Res. 2005;11:6567-73 pubmed
  • western blot; rat
Li Y, Spataro B, Yang J, Dai C, Liu Y. 1,25-dihydroxyvitamin D inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression. Kidney Int. 2005;68:1500-10 pubmed
  • western blot; mouse
Scheef E, Wang S, Sorenson C, Sheibani N. Isolation and characterization of murine retinal astrocytes. Mol Vis. 2005;11:613-24 pubmed
  • immunohistochemistry; rat
Vallbo C, Damber J. Thrombospondins, metallo proteases and thrombospondin receptors messenger RNA and protein expression in different tumour sublines of the Dunning prostate cancer model. Acta Oncol. 2005;44:293-8 pubmed
  • immunohistochemistry; rat
Suzuki K, Wang R, Kubota H, Shibuya H, Saegusa J, Sato T. Kinetics of biglycan, decorin and thrombospondin-1 in mercuric chloride-induced renal tubulointerstitial fibrosis. Exp Mol Pathol. 2005;79:68-73 pubmed
  • western blot; human
Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, et al. Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch. Am J Pathol. 2005;166:1883-94 pubmed
  • ELISA; human; 1:500
Isenberg J, Calzada M, Zhou L, Guo N, Lawler J, Wang X, et al. Endogenous thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor. Matrix Biol. 2005;24:110-23 pubmed
  • immunohistochemistry; mouse
Uno K, Hayashi H, Uchida H, Kuroki M, Oshima K. Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice. Histol Histopathol. 2005;20:493-9 pubmed publisher
  • immunohistochemistry; mouse
Olson L, Zhang J, Taylor H, Rose D, Rosenfeld M. Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling. Proc Natl Acad Sci U S A. 2005;102:3708-13 pubmed
  • western blot; rat
Bouvet C, Gilbert L, Girardot D, Deblois D, Moreau P. Different involvement of extracellular matrix components in small and large arteries during chronic NO synthase inhibition. Hypertension. 2005;45:432-7 pubmed
  • immunoprecipitation; rat
  • immunohistochemistry; rat
  • western blot; rat
Ihara Y, Manabe S, Kanda M, Kawano H, Nakayama T, Sekine I, et al. Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats. Glycobiology. 2005;15:383-92 pubmed
Lemieux P, Roudier E, Birot O. Angiostatic freeze or angiogenic move? Acute cold stress prevents angiokine secretion from murine myotubes but primes primary endothelial cells for greater migratory capacity. Front Physiol. 2022;13:975652 pubmed publisher
Guo B, Friedland S, Alexander W, Myers J, Wang W, O Dell M, et al. Arid1a mutation suppresses TGF-β signaling and induces cholangiocarcinoma. Cell Rep. 2022;40:111253 pubmed publisher
Feng Q, Gu J, Chen J, Zheng W, Pan H, Xu X, et al. TSP1 promotes fibroblast proliferation and extracellular matrix deposition via the IL6/JAK2/STAT3 signalling pathway in keloids. Exp Dermatol. 2022;31:1533-1542 pubmed publisher
Wang B, Li X, Yu N, Yang L, Nan C, Guo L, et al. Intracerebral hemorrhage alters α2δ1 and thrombospondin expression in rats. Exp Ther Med. 2022;23:327 pubmed publisher
Yeung V, Zhang T, Yuan L, Parekh M, Cortinas J, Delavogia E, et al. Extracellular Vesicles Secreted by Corneal Myofibroblasts Promote Corneal Epithelial Cell Migration. Int J Mol Sci. 2022;23: pubmed publisher
Shiau A, Fang S, Hsu C, Chiu M, Lam C, Wu C, et al. Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury. Acta Cardiol Sin. 2022;38:187-200 pubmed publisher
Sterken B, Ackermann T, M xfc ller C, Zuidhof H, Kortman G, Hernandez Segura A, et al. C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells. NPJ Breast Cancer. 2022;8:11 pubmed publisher
Wallis S, Wolska N, Englert H, Posner M, Upadhyay A, Renn xe9 T, et al. A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes. J Thromb Haemost. 2022;20:729-741 pubmed publisher
Delaunay K, Sellam A, Dinet V, Moulin A, Zhao M, Gélizé E, et al. Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration. J Clin Med. 2021;10: pubmed publisher
Chung J, Jeong D, Kim G, Go S, Song J, Moon E, et al. Super-resolution imaging of platelet-activation process and its quantitative analysis. Sci Rep. 2021;11:10511 pubmed publisher
Lam B, Nwadozi E, Haas T, Birot O, Roudier E. High Glucose Treatment Limits Drosha Protein Expression and Alters AngiomiR Maturation in Microvascular Primary Endothelial Cells via an Mdm2-dependent Mechanism. Cells. 2021;10: pubmed publisher
Ferrara M, Chialli G, Ferreira L, Ruggieri E, Careccia G, Preti A, et al. Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions. Front Immunol. 2020;11:1122 pubmed publisher
Federico A, Steinfass T, Larribere L, Novak D, Morís F, Núñez L, et al. Mithramycin A and Mithralog EC-8042 Inhibit SETDB1 Expression and Its Oncogenic Activity in Malignant Melanoma. Mol Ther Oncolytics. 2020;18:83-99 pubmed publisher
Perez Boza J, Boeckx A, Lion M, Dequiedt F, Struman I. hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells. Cell Mol Life Sci. 2020;77:4413-4428 pubmed publisher
Esteban S, Clemente C, Koziol A, Gonzalo P, Rius C, Martinez F, et al. Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis. EMBO Mol Med. 2020;12:e10862 pubmed publisher
Waddingham M, Sonobe T, Tsuchimochi H, Edgley A, Sukumaran V, Chen Y, et al. Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model. J Mol Cell Cardiol. 2019;137:119-131 pubmed publisher
Seif K, Alidzanovic L, Tischler B, Ibrahim N, Zagrapan B, Rauscher S, et al. Neutrophil-Mediated Proteolysis of Thrombospondin-1 Promotes Platelet Adhesion and String Formation. Thromb Haemost. 2018;118:2074-2085 pubmed publisher
Aiken J, Mandel E, Riddell M, Birot O. Hyperglycaemia correlates with skeletal muscle capillary regression and is associated with alterations in the murine double minute-2/forkhead box O1/thrombospondin-1 pathway in type 1 diabetic BioBreeding rats. Diab Vasc Dis Res. 2019;16:28-37 pubmed publisher
de Back D, Nezjad S, Beuger B, Veldhuis M, Clifford E, Ait Ichou F, et al. Apheresis causes complement deposition on red blood cells (RBCs) and RBC antigen alterations, possibly inducing enhanced clearance. Transfusion. 2018;58:2627-2634 pubmed publisher
Farnoodian M, Sorenson C, Sheibani N. PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells. Am J Physiol Cell Physiol. 2018;314:C456-C472 pubmed publisher
Martín Granado V, Ortiz Rivero S, Carmona R, Gutiérrez Herrero S, Barrera M, San Segundo L, et al. C3G promotes a selective release of angiogenic factors from activated mouse platelets to regulate angiogenesis and tumor metastasis. Oncotarget. 2017;8:110994-111011 pubmed publisher
Diaz A, Merino P, Manrique L, Ospina J, Cheng L, Wu F, et al. A Cross Talk between Neuronal Urokinase-type Plasminogen Activator (uPA) and Astrocytic uPA Receptor (uPAR) Promotes Astrocytic Activation and Synaptic Recovery in the Ischemic Brain. J Neurosci. 2017;37:10310-10322 pubmed publisher
Talme T, Bergdahl E, Sundqvist K. Methotrexate and its therapeutic antagonists caffeine and theophylline, target a motogenic T-cell mechanism driven by thrombospondin-1 (TSP-1). Eur J Immunol. 2016;46:1279-90 pubmed publisher
Krencik R, Hokanson K, Narayan A, Dvornik J, Rooney G, Rauen K, et al. Dysregulation of astrocyte extracellular signaling in Costello syndrome. Sci Transl Med. 2015;7:286ra66 pubmed publisher
Farnoodian M, Kinter J, Yadranji Aghdam S, Zaitoun I, Sorenson C, Sheibani N. Expression of pigment epithelium-derived factor and thrombospondin-1 regulate proliferation and migration of retinal pigment epithelial cells. Physiol Rep. 2015;3: pubmed publisher
Audet G, Fulks D, Stricker J, Olfert I. Chronic delivery of a thrombospondin-1 mimetic decreases skeletal muscle capillarity in mice. PLoS ONE. 2013;8:e55953 pubmed publisher
Chandler R, Brennan J, Schisler J, Serber D, Patterson C, Magnuson T. ARID1a-DNA interactions are required for promoter occupancy by SWI/SNF. Mol Cell Biol. 2013;33:265-80 pubmed publisher
Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, et al. Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer. Int J Cancer. 2011;129:2134-46 pubmed publisher
Ali N, Gaughan A, Orosz C, Baran C, McMaken S, Wang Y, et al. Latency associated peptide has in vitro and in vivo immune effects independent of TGF-beta1. PLoS ONE. 2008;3:e1914 pubmed publisher
Li S, Forslöw A, Sundqvist K. Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction. J Immunol. 2005;174:654-61 pubmed
Flügel Koch C, Ohlmann A, Fuchshofer R, Welge Lussen U, Tamm E. Thrombospondin-1 in the trabecular meshwork: localization in normal and glaucomatous eyes, and induction by TGF-beta1 and dexamethasone in vitro. Exp Eye Res. 2004;79:649-63 pubmed
Ziehr J, Sheibani N, Sorenson C. Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice. Am J Physiol Renal Physiol. 2004;287:F1154-63 pubmed
Sengupta K, Banerjee S, Saxena N, Banerjee S. Thombospondin-1 disrupts estrogen-induced endothelial cell proliferation and migration and its expression is suppressed by estradiol. Mol Cancer Res. 2004;2:150-8 pubmed
Hochegger K, Knight S, Hugo C, Mayer G, Lawler J, Mayadas T, et al. Role of thrombospondin-1 in the autologous phase of an accelerated model of anti-glomerular basement membrane glomerulonephritis. Nephron Exp Nephrol. 2004;96:e31-8 pubmed
Uno K, Hayashi H, Kuroki M, Uchida H, Yamauchi Y, Kuroki M, et al. Thrombospondin-1 accelerates wound healing of corneal epithelia. Biochem Biophys Res Commun. 2004;315:928-34 pubmed
Qin H, Shao Q, Thomas T, Kalra J, Alaoui Jamali M, Laird D. Connexin26 regulates the expression of angiogenesis-related genes in human breast tumor cells by both GJIC-dependent and -independent mechanisms. Cell Commun Adhes. 2003;10:387-93 pubmed
Salvesen H, Gulluoglu M, Stefansson I, Akslen L. Significance of CD 105 expression for tumour angiogenesis and prognosis in endometrial carcinomas. APMIS. 2003;111:1011-8 pubmed
Liu X, Wu Z, Sheibani N, Brandt C, Polansky J, Kaufman P. Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells. Exp Eye Res. 2003;77:181-8 pubmed
Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt Clermont P, Kovacic H. Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol Cell Biol. 2003;23:5401-8 pubmed
Alaoui Jamali M, Song D, Benlimame N, Yen L, Deng X, Hernandez Perez M, et al. Regulation of multiple tumor microenvironment markers by overexpression of single or paired combinations of ErbB receptors. Cancer Res. 2003;63:3764-74 pubmed
Stenina O, Krukovets I, Wang K, Zhou Z, Forudi F, Penn M, et al. Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat. Circulation. 2003;107:3209-15 pubmed
Su X, Sorenson C, Sheibani N. Isolation and characterization of murine retinal endothelial cells. Mol Vis. 2003;9:171-8 pubmed
Rice A, Steward M, Quinn C. Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast. J Clin Pathol. 2002;55:921-5 pubmed
Burnett T, Tash J, Hunt J. Investigation of the role of nitric oxide synthase 2 in pregnancy using mutant mice. Reproduction. 2002;124:49-57 pubmed
Mattern J, Koomägi R, Volm M. Characteristics of long-term survivors of untreated lung cancer. Lung Cancer. 2002;36:277-82 pubmed
Li S, Ivanoff A, Bergström S, Sandström A, Christensson B, van Nerven J, et al. T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components. Eur J Immunol. 2002;32:1069-79 pubmed
Kragh M, Quistorff B, Tenan M, Van Meir E, Kristjansen P. Overexpression of thrombospondin-1 reduces growth and vascular index but not perfusion in glioblastoma. Cancer Res. 2002;62:1191-5 pubmed
Filla M, Liu X, Nguyen T, Polansky J, Brandt C, Kaufman P, et al. In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin. Invest Ophthalmol Vis Sci. 2002;43:151-61 pubmed
Rofstad E, Graff B. Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts. J Invest Dermatol. 2001;117:1042-9 pubmed
Zhao X, Hu Y, Miller G, Mitchell R, Libby P. Association of thrombospondin-1 and cardiac allograft vasculopathy in human cardiac allografts. Circulation. 2001;103:525-31 pubmed
Tenan M, Fulci G, Albertoni M, Diserens A, Hamou M, El Atifi Borel M, et al. Thrombospondin-1 is downregulated by anoxia and suppresses tumorigenicity of human glioblastoma cells. J Exp Med. 2000;191:1789-98 pubmed
Shi S, Cote R, Hawes D, Thu S, Shi Y, Young L, et al. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?. J Histochem Cytochem. 1999;47:463-70 pubmed
image
image 1 :
Invitrogen MA5-13398 image 1
image 2 :
Invitrogen MA5-13398 image 2
image 3 :
Invitrogen MA5-13398 image 3
product information
Product Type :
Antibody
Product Name :
Thrombospondin 1 Monoclonal Antibody (A6.1)
Catalog # :
MA5-13398
Quantity :
200 µL
Price :
US 425.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Bovine, Canine, Equine, Human, Mouse, Ovine, Porcine, Rat
Applications :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:25-1:50, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
Species :
Bovine, Canine, Equine, Human, Mouse, Ovine, Porcine, Rat
Clone :
A6.1
Isotype :
IgG1
Storage :
4° C
Description :
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Immunogen :
Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:25-1:50, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
Aliases :
glycoprotein G; tbsp1; THBS; Thbs1; Thbs-1; thrombospondin; thrombospondin 1; thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1; TSP; TSP1; TSP-1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA