Thrombospondin 1 Monoclonal Antibody (A6.1), Biotin | Invitrogen MA5-13395 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Thrombospondin 1 Monoclonal Antibody (A6.1), Biotin

catalog :

MA5-13395

quantity :

500 µL

price :

US 500.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

A6.1

reactivity :

human, mouse, rat, dogs, bovine, pigs , domestic sheep, domestic horse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 111

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5f	Engelbrecht E, Lévesque M, He L, Vanlandewijck M, Nitzsche A, Niazi H, et al. Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta. elife. 2020;9: pubmed publisher
western blot; human	Talme T, Bergdahl E, Sundqvist K. Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1. Immunology. 2014;142:176-92 pubmed publisher
immunohistochemistry; rat	Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C. Long-term gene therapy with thrombospondin 2 inhibits TGF-? activation, inflammation and angiogenesis in chronic allograft nephropathy. PLoS ONE. 2013;8:e83846 pubmed publisher
western blot; mouse	Morrison M, Palenski T, Jamali N, Sheibani N, Sorenson C. Modulation of vascular cell function by bim expression. Int J Cell Biol. 2013;2013:297537 pubmed publisher
western blot; mouse; 1:1000	Zhao Y, Wang S, Sorenson C, Teixeira L, Dubielzig R, Peters D, et al. Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress. Mol Cell Biol. 2013;33:4225-40 pubmed publisher
western blot; human	Bergström S, Bergdahl E, Sundqvist K. A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation. Immunology. 2013;140:441-55 pubmed publisher
western blot; mouse	Palenski T, Sorenson C, Sheibani N. Inflammatory cytokine-specific alterations in retinal endothelial cell function. Microvasc Res. 2013;89:57-69 pubmed publisher
western blot; mouse	Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-? signaling pathways. J Cell Sci. 2013;126:1392-405 pubmed publisher
ELISA; human; 1:500	Martin Manso G, Navarathna D, Galli S, Soto Pantoja D, Kuznetsova S, Tsokos M, et al. Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis. PLoS ONE. 2012;7:e48775 pubmed publisher
immunohistochemistry; human; 1:100	Alkim C, Sakiz D, Alkim H, Livaoglu A, Kendir T, Demirsoy H, et al. Thrombospondin-1 and VEGF in inflammatory bowel disease. Libyan J Med. 2012;7: pubmed publisher
western blot; rat	Kessler Icekson G, Hochhauser E, Sinai T, Kremer A, Dick J, Tarasenko N, et al. A histone deacetylase inhibitory prodrug - butyroyloxymethyl diethyl phosphate - protects the heart and cardiomyocytes against ischemia injury. Eur J Pharm Sci. 2012;45:592-9 pubmed publisher
western blot; mouse	Sheibani N, Morrison M, Gurel Z, Park S, Sorenson C. BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment. Am J Physiol Renal Physiol. 2012;302:F809-19 pubmed publisher
immunohistochemistry; rat	Matsuba M, Hutcheon A, Zieske J. Localization of thrombospondin-1 and myofibroblasts during corneal wound repair. Exp Eye Res. 2011;93:534-40 pubmed publisher
western blot; human; 1:400	El Btaouri H, Morjani H, Greffe Y, Charpentier E, Martiny L. Role of JNK/ATF-2 pathway in inhibition of thrombospondin-1 (TSP-1) expression and apoptosis mediated by doxorubicin and camptothecin in FTC-133 cells. Biochim Biophys Acta. 2011;1813:695-703 pubmed publisher
western blot; human; 1:500	Patten S, Adamcic U, Lacombe K, Minhas K, Skowronski K, Coomber B. VEGFR2 heterogeneity and response to anti-angiogenic low dose metronomic cyclophosphamide treatment. BMC Cancer. 2010;10:683 pubmed publisher
western blot; human	Dews M, Fox J, Hultine S, Sundaram P, Wang W, Liu Y, et al. The myc-miR-17~92 axis blunts TGF{beta} signaling and production of multiple TGF{beta}-dependent antiangiogenic factors. Cancer Res. 2010;70:8233-46 pubmed publisher
western blot; rat	Ikeda H, Miyatake M, Koshikawa N, Ochiai K, Yamada K, Kiss A, et al. Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation. J Biol Chem. 2010;285:38415-27 pubmed publisher
immunohistochemistry; mouse	Wang Y, Cheng W, Ke Y, Cai Z, Chen L, Xi Y, et al. Effect of Qingre Quyu Granule (??????) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice. Chin J Integr Med. 2010;16:442-7 pubmed publisher
western blot; rat	Tarasenko N, Kessler Icekson G, Boer P, Inbal A, Schlesinger H, Phillips D, et al. The histone deacetylase inhibitor butyroyloxymethyl diethylphosphate (AN-7) protects normal cells against toxicity of anticancer agents while augmenting their anticancer activity. Invest New Drugs. 2012;30:130-43 pubmed publisher
western blot; mouse; 1:1000	Park S, Dimaio T, Scheef E, Sorenson C, Sheibani N. PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions. Am J Physiol Cell Physiol. 2010;299:C1468-84 pubmed publisher
immunohistochemistry; human; 1:50	Guerrero D, Guarch R, Ojer A, Casas J, Méndez Meca C, Esteller M, et al. Differential hypermethylation of genes in vulvar cancer and lichen sclerosus coexisting or not with vulvar cancer. Int J Cancer. 2011;128:2853-64 pubmed publisher
western blot; human; 1:250	Hohberg M, Knöchel J, Hoffmann C, Chlench S, Wunderlich W, Alter A, et al. Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries. J Cell Physiol. 2011;226:350-61 pubmed publisher
western blot; mouse	Grutzmacher C, Park S, Elmergreen T, Tang Y, Scheef E, Sheibani N, et al. Opposing effects of bim and bcl-2 on lung endothelial cell migration. Am J Physiol Lung Cell Mol Physiol. 2010;299:L607-20 pubmed publisher
immunohistochemistry; mouse	Lanz T, Ding Z, Ho P, Luo J, Agrawal A, Srinagesh H, et al. Angiotensin II sustains brain inflammation in mice via TGF-beta. J Clin Invest. 2010;120:2782-94 pubmed publisher
western blot; human	Tan K, Lessieur E, Cutler A, Nerone P, Vasanji A, Asosingh K, et al. Impaired function of circulating CD34(+) CD45(-) cells in patients with proliferative diabetic retinopathy. Exp Eye Res. 2010;91:229-37 pubmed publisher
western blot; human	Olsson A, Björk A, Vallon Christersson J, Isaacs J, Leanderson T. Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors. Mol Cancer. 2010;9:107 pubmed publisher
immunohistochemistry; human western blot; human	Haldar S, Lu Y, Jeyaraj D, Kawanami D, Cui Y, Eapen S, et al. Klf15 deficiency is a molecular link between heart failure and aortic aneurysm formation. Sci Transl Med. 2010;2:26ra26 pubmed publisher
immunocytochemistry; mouse; 1:70 immunohistochemistry; mouse; 1:70 western blot; mouse; 1:200	Yonezawa T, Hattori S, Inagaki J, Kurosaki M, Takigawa T, Hirohata S, et al. Type IV collagen induces expression of thrombospondin-1 that is mediated by integrin alpha1beta1 in astrocytes. Glia. 2010;58:755-67 pubmed publisher
immunohistochemistry; human; 1:500	Zaslavsky A, Baek K, Lynch R, Short S, Grillo J, Folkman J, et al. Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis. Blood. 2010;115:4605-13 pubmed publisher
immunoprecipitation; mouse; 2 ug/time immunohistochemistry; mouse; 1:50 western blot; mouse	Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls J, et al. The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1. J Biol Chem. 2010;285:6770-80 pubmed publisher
immunohistochemistry; rat	Daniel C, Wagner A, Hohenstein B, Hugo C. Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation. Am J Physiol Renal Physiol. 2009;297:F1299-309 pubmed publisher
immunohistochemistry; mouse; 1:100	Park C, Kim H, Lim J, Yoo K, Chung S, Shin S, et al. Vascular endothelial growth factor inhibition by dRK6 causes endothelial apoptosis, fibrosis, and inflammation in the heart via the Akt/eNOS axis in db/db mice. Diabetes. 2009;58:2666-76 pubmed publisher
immunoprecipitation; human western blot; human	Rex S, Beaulieu L, Perlman D, Vitseva O, Blair P, McComb M, et al. Immune versus thrombotic stimulation of platelets differentially regulates signalling pathways, intracellular protein-protein interactions, and alpha-granule release. Thromb Haemost. 2009;102:97-110 pubmed publisher
immunohistochemistry; rat	Szasz T, Eddy S, Paulauskis J, Burnett R, Ellekilde M, Iovanna J, et al. Differential expression of pancreatitis-associated protein and thrombospondins in arterial versus venous tissues. J Vasc Res. 2009;46:551-60 pubmed publisher
western blot; human; 1:200	Hyder S, Liang Y, Wu J, Welbern V. Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells. Endocr Relat Cancer. 2009;16:809-17 pubmed publisher
blocking or activating experiments; mouse	Isenberg J, Shiva S, Gladwin M. Thrombospondin-1-CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO-cGMP pathway activation. Nitric Oxide. 2009;21:52-62 pubmed publisher
western blot; human; 1:200	Hyder S, Liang Y, Wu J. Estrogen regulation of thrombospondin-1 in human breast cancer cells. Int J Cancer. 2009;125:1045-53 pubmed publisher
western blot; human; 1:400	Scheef E, Sorenson C, Sheibani N. Attenuation of proliferation and migration of retinal pericytes in the absence of thrombospondin-1. Am J Physiol Cell Physiol. 2009;296:C724-34 pubmed publisher
immunohistochemistry; mouse; 1:100	Isenberg J, Maxhimer J, Powers P, Tsokos M, Frazier W, Roberts D. Treatment of liver ischemia-reperfusion injury by limiting thrombospondin-1/CD47 signaling. Surgery. 2008;144:752-61 pubmed publisher
western blot; mouse	Huang Q, Sheibani N. High glucose promotes retinal endothelial cell migration through activation of Src, PI3K/Akt1/eNOS, and ERKs. Am J Physiol Cell Physiol. 2008;295:C1647-57 pubmed publisher
blocking or activating experiments; human	Gayen Betal S, Setty B. Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain. Transl Res. 2008;152:165-77 pubmed publisher
blocking or activating experiments; rat; 20 ug/ml	Anderson C, Hastings N, Blackman B, Price R. Capillary sprout endothelial cells exhibit a CD36 low phenotype: regulation by shear stress and vascular endothelial growth factor-induced mechanism for attenuating anti-proliferative thrombospondin-1 signaling. Am J Pathol. 2008;173:1220-8 pubmed publisher
immunohistochemistry; mouse; 8 ug/ml	Gruber H, Bornstein P, Sage E, Ingram J, Zinchenko N, Norton H, et al. Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc. Arthritis Res Ther. 2008;10:R96 pubmed publisher
western blot; human	Mazan Mamczarz K, Hagner P, Corl S, Srikantan S, Wood W, Becker K, et al. Post-transcriptional gene regulation by HuR promotes a more tumorigenic phenotype. Oncogene. 2008;27:6151-63 pubmed publisher
immunohistochemistry; human	Ide M, Ishii H, Mukae H, Iwata A, Sakamoto N, Kadota J, et al. High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia. Respir Med. 2008;102:1625-30 pubmed publisher
immunohistochemistry; mouse; 1:400	Johnson A, O Connell M, Miyamoto H, Huang J, Yao J, Messing E, et al. Androgenic dependence of exophytic tumor growth in a transgenic mouse model of bladder cancer: a role for thrombospondin-1. BMC Urol. 2008;8:7 pubmed publisher
immunohistochemistry; human; 1:2000 western blot; human	Rojas A, Meherem S, Kim Y, Washington M, Willis J, Markowitz S, et al. The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer. Int J Cancer. 2008;123:14-21 pubmed publisher
western blot; mouse	Olerud J, Johansson M, Lawler J, Welsh N, Carlsson P. Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets. Diabetes. 2008;57:1870-7 pubmed publisher
western blot; mouse	Kondo S, Tang Y, Scheef E, Sheibani N, Sorenson C. Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2. Am J Physiol Cell Physiol. 2008;294:C1521-30 pubmed publisher
immunohistochemistry; human; 1:50	Guerrero D, Guarch R, Ojer A, Casas J, Ropero S, Mancha A, et al. Hypermethylation of the thrombospondin-1 gene is associated with poor prognosis in penile squamous cell carcinoma. BJU Int. 2008;102:747-55 pubmed publisher
immunoprecipitation; human	Bhattacharyya S, Marinic T, Krukovets I, Hoppe G, Stenina O. Cell type-specific post-transcriptional regulation of production of the potent antiangiogenic and proatherogenic protein thrombospondin-1 by high glucose. J Biol Chem. 2008;283:5699-707 pubmed
immunohistochemistry; human western blot; human	Park J, Lee H, Ko K, Park S, Kim J, Choe G, et al. Meteorin regulates angiogenesis at the gliovascular interface. Glia. 2008;56:247-58 pubmed
western blot; mouse	Scheef E, Huang Q, Wang S, Sorenson C, Sheibani N. Isolation and characterization of corneal endothelial cells from wild type and thrombospondin-1 deficient mice. Mol Vis. 2007;13:1483-95 pubmed
blocking or activating experiments; human	Derks R, Jankowska Gan E, Xu Q, Burlingham W. Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1. J Immunol. 2007;179:3443-51 pubmed
blocking or activating experiments; human; 1:1000 western blot; human	Hong S, Chang S, Yeom D, Kang J, Hong K. Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A. Anticancer Drugs. 2007;18:1005-14 pubmed
immunohistochemistry; domestic sheep; 1:50	Sozo F, Hooper S, Wallace M. Thrombospondin-1 expression and localization in the developing ovine lung. J Physiol. 2007;584:625-35 pubmed
ELISA; human	Annis D, Gunderson K, Mosher D. Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations. J Biol Chem. 2007;282:27067-75 pubmed
immunohistochemistry; human	Pohlers D, Beyer A, Koczan D, Wilhelm T, Thiesen H, Kinne R. Constitutive upregulation of the transforming growth factor-beta pathway in rheumatoid arthritis synovial fibroblasts. Arthritis Res Ther. 2007;9:R59 pubmed
western blot; mouse; 1:3000	Kondo S, Scheef E, Sheibani N, Sorenson C. PECAM-1 isoform-specific regulation of kidney endothelial cell migration and capillary morphogenesis. Am J Physiol Cell Physiol. 2007;292:C2070-83 pubmed
immunohistochemistry; rat	de Nigris F, Balestrieri M, Williams Ignarro S, D Armiento F, Fiorito C, Ignarro L, et al. The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats. Nitric Oxide. 2007;17:50-4 pubmed
immunohistochemistry; mouse	Kaipainen A, Kieran M, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, et al. PPARalpha deficiency in inflammatory cells suppresses tumor growth. PLoS ONE. 2007;2:e260 pubmed
immunohistochemistry; mouse	Isenberg J, Romeo M, Abu Asab M, Tsokos M, Oldenborg A, Pappan L, et al. Increasing survival of ischemic tissue by targeting CD47. Circ Res. 2007;100:712-20 pubmed
immunocytochemistry; human; 2 ug/ml	Raman P, Krukovets I, Marinic T, Bornstein P, Stenina O. Glycosylation mediates up-regulation of a potent antiangiogenic and proatherogenic protein, thrombospondin-1, by glucose in vascular smooth muscle cells. J Biol Chem. 2007;282:5704-14 pubmed
immunohistochemistry; human	Kopp H, Hooper A, Broekman M, Avecilla S, Petit I, Luo M, et al. Thrombospondins deployed by thrombopoietic cells determine angiogenic switch and extent of revascularization. J Clin Invest. 2006;116:3277-91 pubmed
western blot; mouse; 1:4000	Sheibani N, Scheef E, Dimaio T, Wang Y, Kondo S, Sorenson C. Bcl-2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells. J Cell Physiol. 2007;210:616-25 pubmed
immunohistochemistry; mouse; 1:25	Isenberg J, Hyodo F, Matsumoto K, Romeo M, Abu Asab M, Tsokos M, et al. Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide-mediated vascular smooth muscle relaxation. Blood. 2007;109:1945-52 pubmed
immunohistochemistry; mouse; 1:50	Giuriato S, Ryeom S, Fan A, Bachireddy P, Lynch R, Rioth M, et al. Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch. Proc Natl Acad Sci U S A. 2006;103:16266-71 pubmed
immunocytochemistry; human	Kuznetsova S, Issa P, Perruccio E, Zeng B, Sipes J, Ward Y, et al. Versican-thrombospondin-1 binding in vitro and colocalization in microfibrils induced by inflammation on vascular smooth muscle cells. J Cell Sci. 2006;119:4499-509 pubmed
immunohistochemistry; mouse; 1:20	Abeles D, Kwei S, Stavrakis G, Zhang Y, Wang E, Garcia Cardena G. Gene expression changes evoked in a venous segment exposed to arterial flow. J Vasc Surg. 2006;44:863-70 pubmed
immunohistochemistry; human; 1:500	Duvic M, Talpur R, Ni X, Zhang C, Hazarika P, Kelly C, et al. Phase 2 trial of oral vorinostat (suberoylanilide hydroxamic acid, SAHA) for refractory cutaneous T-cell lymphoma (CTCL). Blood. 2007;109:31-9 pubmed
immunohistochemistry; mouse; 1:50	Shoji T, Koyama H, Morioka T, Tanaka S, Kizu A, Motoyama K, et al. Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes. Diabetes. 2006;55:2245-55 pubmed
immunohistochemistry; human; 4 ug/ml	Wen X, Yang G, Mao W, Thornton A, Liu J, Bast R, et al. HER2 signaling modulates the equilibrium between pro- and antiangiogenic factors via distinct pathways: implications for HER2-targeted antibody therapy. Oncogene. 2006;25:6986-96 pubmed
western blot; mouse	Wang Y, Wang S, Sheibani N. Enhanced proangiogenic signaling in thrombospondin-1-deficient retinal endothelial cells. Microvasc Res. 2006;71:143-51 pubmed
immunohistochemistry; human; 1:50	Bodner Adler B, Nather A, Bodner K, Czerwenka K, Kimberger O, Leodolter S, et al. Expression of thrombospondin 1 (TSP 1) in patients with uterine smooth muscle tumors: an immunohistochemical study. Gynecol Oncol. 2006;103:186-9 pubmed
immunohistochemistry; rat	Johansson M, Mattsson G, Andersson A, Jansson L, Carlsson P. Islet endothelial cells and pancreatic beta-cell proliferation: studies in vitro and during pregnancy in adult rats. Endocrinology. 2006;147:2315-24 pubmed
immunohistochemistry; human; 1,000 ug/ml western blot; human; 1:200	Jo W, Mizukami Y, Duerr E, Zukerberg L, Chung D. Wnt signaling can repress thrombospondin-1 expression in colonic tumorigenesis. Cancer Biol Ther. 2005;4:1361-6 pubmed
immunohistochemistry; human; 1:50	Sutton C, O Byrne K, Goddard J, Marshall L, Jones L, Garcea G, et al. Expression of thrombospondin-1 in resected colorectal liver metastases predicts poor prognosis. Clin Cancer Res. 2005;11:6567-73 pubmed
western blot; rat	Li Y, Spataro B, Yang J, Dai C, Liu Y. 1,25-dihydroxyvitamin D inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression. Kidney Int. 2005;68:1500-10 pubmed
western blot; mouse	Scheef E, Wang S, Sorenson C, Sheibani N. Isolation and characterization of murine retinal astrocytes. Mol Vis. 2005;11:613-24 pubmed
immunohistochemistry; rat	Vallbo C, Damber J. Thrombospondins, metallo proteases and thrombospondin receptors messenger RNA and protein expression in different tumour sublines of the Dunning prostate cancer model. Acta Oncol. 2005;44:293-8 pubmed
immunohistochemistry; rat	Suzuki K, Wang R, Kubota H, Shibuya H, Saegusa J, Sato T. Kinetics of biglycan, decorin and thrombospondin-1 in mercuric chloride-induced renal tubulointerstitial fibrosis. Exp Mol Pathol. 2005;79:68-73 pubmed
western blot; human	Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, et al. Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch. Am J Pathol. 2005;166:1883-94 pubmed
ELISA; human; 1:500	Isenberg J, Calzada M, Zhou L, Guo N, Lawler J, Wang X, et al. Endogenous thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor. Matrix Biol. 2005;24:110-23 pubmed
immunohistochemistry; mouse	Uno K, Hayashi H, Uchida H, Kuroki M, Oshima K. Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice. Histol Histopathol. 2005;20:493-9 pubmed publisher
immunohistochemistry; mouse	Olson L, Zhang J, Taylor H, Rose D, Rosenfeld M. Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling. Proc Natl Acad Sci U S A. 2005;102:3708-13 pubmed
western blot; rat	Bouvet C, Gilbert L, Girardot D, Deblois D, Moreau P. Different involvement of extracellular matrix components in small and large arteries during chronic NO synthase inhibition. Hypertension. 2005;45:432-7 pubmed
immunoprecipitation; rat immunohistochemistry; rat western blot; rat	Ihara Y, Manabe S, Kanda M, Kawano H, Nakayama T, Sekine I, et al. Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats. Glycobiology. 2005;15:383-92 pubmed
	Vanhoutte D, Schips T, Vo A, Grimes K, Baldwin T, Brody M, et al. Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy. Nat Commun. 2021;12:3928 pubmed publisher
	Li S, Forslöw A, Sundqvist K. Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction. J Immunol. 2005;174:654-61 pubmed
	Flügel Koch C, Ohlmann A, Fuchshofer R, Welge Lussen U, Tamm E. Thrombospondin-1 in the trabecular meshwork: localization in normal and glaucomatous eyes, and induction by TGF-beta1 and dexamethasone in vitro. Exp Eye Res. 2004;79:649-63 pubmed
	Ziehr J, Sheibani N, Sorenson C. Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice. Am J Physiol Renal Physiol. 2004;287:F1154-63 pubmed
	Sengupta K, Banerjee S, Saxena N, Banerjee S. Thombospondin-1 disrupts estrogen-induced endothelial cell proliferation and migration and its expression is suppressed by estradiol. Mol Cancer Res. 2004;2:150-8 pubmed
	Hochegger K, Knight S, Hugo C, Mayer G, Lawler J, Mayadas T, et al. Role of thrombospondin-1 in the autologous phase of an accelerated model of anti-glomerular basement membrane glomerulonephritis. Nephron Exp Nephrol. 2004;96:e31-8 pubmed
	Uno K, Hayashi H, Kuroki M, Uchida H, Yamauchi Y, Kuroki M, et al. Thrombospondin-1 accelerates wound healing of corneal epithelia. Biochem Biophys Res Commun. 2004;315:928-34 pubmed
	Qin H, Shao Q, Thomas T, Kalra J, Alaoui Jamali M, Laird D. Connexin26 regulates the expression of angiogenesis-related genes in human breast tumor cells by both GJIC-dependent and -independent mechanisms. Cell Commun Adhes. 2003;10:387-93 pubmed
	Salvesen H, Gulluoglu M, Stefansson I, Akslen L. Significance of CD 105 expression for tumour angiogenesis and prognosis in endometrial carcinomas. APMIS. 2003;111:1011-8 pubmed
	Liu X, Wu Z, Sheibani N, Brandt C, Polansky J, Kaufman P. Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells. Exp Eye Res. 2003;77:181-8 pubmed
	Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt Clermont P, Kovacic H. Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol Cell Biol. 2003;23:5401-8 pubmed
	Alaoui Jamali M, Song D, Benlimame N, Yen L, Deng X, Hernandez Perez M, et al. Regulation of multiple tumor microenvironment markers by overexpression of single or paired combinations of ErbB receptors. Cancer Res. 2003;63:3764-74 pubmed
	Stenina O, Krukovets I, Wang K, Zhou Z, Forudi F, Penn M, et al. Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat. Circulation. 2003;107:3209-15 pubmed
	Su X, Sorenson C, Sheibani N. Isolation and characterization of murine retinal endothelial cells. Mol Vis. 2003;9:171-8 pubmed
	Rice A, Steward M, Quinn C. Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast. J Clin Pathol. 2002;55:921-5 pubmed
	Flügel Koch C, Ohlmann A, Piatigorsky J, Tamm E. Disruption of anterior segment development by TGF-beta1 overexpression in the eyes of transgenic mice. Dev Dyn. 2002;225:111-25 pubmed
	Burnett T, Tash J, Hunt J. Investigation of the role of nitric oxide synthase 2 in pregnancy using mutant mice. Reproduction. 2002;124:49-57 pubmed
	Mattern J, Koomägi R, Volm M. Characteristics of long-term survivors of untreated lung cancer. Lung Cancer. 2002;36:277-82 pubmed
	Li S, Ivanoff A, Bergström S, Sandström A, Christensson B, van Nerven J, et al. T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components. Eur J Immunol. 2002;32:1069-79 pubmed
	Kragh M, Quistorff B, Tenan M, Van Meir E, Kristjansen P. Overexpression of thrombospondin-1 reduces growth and vascular index but not perfusion in glioblastoma. Cancer Res. 2002;62:1191-5 pubmed
	Filla M, Liu X, Nguyen T, Polansky J, Brandt C, Kaufman P, et al. In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin. Invest Ophthalmol Vis Sci. 2002;43:151-61 pubmed
	Rofstad E, Graff B. Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts. J Invest Dermatol. 2001;117:1042-9 pubmed
	Zhao X, Hu Y, Miller G, Mitchell R, Libby P. Association of thrombospondin-1 and cardiac allograft vasculopathy in human cardiac allografts. Circulation. 2001;103:525-31 pubmed
	Shi S, Cote R, Hawes D, Thu S, Shi Y, Young L, et al. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?. J Histochem Cytochem. 1999;47:463-70 pubmed

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image 3 :

Formalin-fixed, paraffin-embedded human placenta stained with Thrombospondin antibody using peroxidase-conjugate and AEC chromogen. Note staining of platelets in the blood vessels.

product information

Product Type :

Antibody

Product Name :

Thrombospondin 1 Monoclonal Antibody (A6.1), Biotin

Catalog # :

MA5-13395

Quantity :

500 µL

Price :

US 500.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Canine, Equine, Human, Mouse, Ovine, Porcine, Rat

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): 1:25-1:50, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Bovine, Canine, Equine, Human, Mouse, Ovine, Porcine, Rat

Clone :

A6.1

Isotype :

IgG1

Storage :

4° C

Description :

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Immunogen :

Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): 1:25-1:50, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

glycoprotein G; tbsp1; THBS; Thbs1; Thbs-1; thrombospondin; thrombospondin 1; thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1; TSP; TSP1; TSP-1

more info or order :

Invitrogen product webpage