Thrombospondin 1 Monoclonal Antibody (A4.1) | Invitrogen MA5-13377 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Thrombospondin 1 Monoclonal Antibody (A4.1)

catalog :

MA5-13377

quantity :

500 µL

price :

US 496

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4.1

reactivity :

human, mouse, rat, chicken, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 37

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human	Baumann M, Giunta C, Krabichler B, Ruschendorf F, Zoppi N, Colombi M, et al. Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss. Am J Hum Genet. 2012;90:201-16 pubmed publisher
western blot; rat	Chen J, Zhan Y, Yang C, Tzeng S. Oxidative stress-induced attenuation of thrombospondin-1 expression in primary rat astrocytes. J Cell Biochem. 2011;112:59-70 pubmed publisher
blocking or activating experiments; human; 10 ug/ml	De Luna N, Gallardo E, Sonnet C, Chazaud B, Dominguez Perles R, Suarez Calvet X, et al. Role of thrombospondin 1 in macrophage inflammation in dysferlin myopathy. J Neuropathol Exp Neurol. 2010;69:643-53 pubmed publisher
blocking or activating experiments; human; 40 ug/ml immunohistochemistry; human western blot; human	Fitchev P, Wcislak S, Lee C, Bergh A, Brendler C, Stellmach V, et al. Thrombospondin-1 regulates the normal prostate in vivo through angiogenesis and TGF-beta activation. Lab Invest. 2010;90:1078-90 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; mouse	Wang S, Lincoln T, Murphy Ullrich J. Glucose downregulation of PKG-I protein mediates increased thrombospondin1-dependent TGF-{beta} activity in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2010;298:C1188-97 pubmed publisher
blocking or activating experiments; rat	Johansson A, Olerud J, Johansson M, Carlsson P. Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation. Transpl Int. 2009;22:1182-8 pubmed
blocking or activating experiments; human	Hyder S, Liang Y, Wu J, Welbern V. Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells. Endocr Relat Cancer. 2009;16:809-17 pubmed publisher
blocking or activating experiments; human; 1:50	Goel H, Moro L, Murphy Ullrich J, Hsieh C, Wu C, Jiang Z, et al. Beta1 integrin cytoplasmic variants differentially regulate expression of the antiangiogenic extracellular matrix protein thrombospondin 1. Cancer Res. 2009;69:5374-82 pubmed publisher
blocking or activating experiments; human; 10 nM	Hyder S, Liang Y, Wu J. Estrogen regulation of thrombospondin-1 in human breast cancer cells. Int J Cancer. 2009;125:1045-53 pubmed publisher
western blot; rat; 1:200	Moore N, Costa L, Shaffer S, Goodlett D, Guizzetti M. Shotgun proteomics implicates extracellular matrix proteins and protease systems in neuronal development induced by astrocyte cholinergic stimulation. J Neurochem. 2009;108:891-908 pubmed publisher
western blot; human	Kang J, Kim M, Chang S, Sim S, Kim M, Jo Y. CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A. J Cell Biochem. 2008;104:1192-203 pubmed publisher
western blot; human	Robinet A, Emonard H, Banyai L, Laronze J, Patthy L, Hornebeck W, et al. Collagen-binding domains of gelatinase A and thrombospondin-derived peptides impede endocytic clearance of active gelatinase A and promote HT1080 fibrosarcoma cell invasion. Life Sci. 2008;82:376-82 pubmed publisher
blocking or activating experiments; human	Park J, Lee H, Ko K, Park S, Kim J, Choe G, et al. Meteorin regulates angiogenesis at the gliovascular interface. Glia. 2008;56:247-58 pubmed
blocking or activating experiments; mouse	Kaipainen A, Kieran M, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, et al. PPARalpha deficiency in inflammatory cells suppresses tumor growth. PLoS ONE. 2007;2:e260 pubmed
blocking or activating experiments; human; 100 ug/ml	Guzik K, Bzowska M, Smagur J, Krupa O, Sieprawska M, Travis J, et al. A new insight into phagocytosis of apoptotic cells: proteolytic enzymes divert the recognition and clearance of polymorphonuclear leukocytes by macrophages. Cell Death Differ. 2007;14:171-82 pubmed
western blot; human	Naumov G, Bender E, Zurakowski D, Kang S, Sampson D, Flynn E, et al. A model of human tumor dormancy: an angiogenic switch from the nonangiogenic phenotype. J Natl Cancer Inst. 2006;98:316-25 pubmed
western blot; mouse western blot; human	Kang J, Kim S, Hong K. Induction of TSP1 gene expression by heat shock is mediated via an increase in mRNA stability. FEBS Lett. 2006;580:510-6 pubmed
immunohistochemistry; pigs	Kermorvant Duchemin E, Sennlaub F, Sirinyan M, Brault S, Andelfinger G, Kooli A, et al. Trans-arachidonic acids generated during nitrative stress induce a thrombospondin-1-dependent microvascular degeneration. Nat Med. 2005;11:1339-45 pubmed
blocking or activating experiments; human	Hasina R, Pontier A, Fekete M, Martin L, Qi X, Brigaudeau C, et al. NOL7 is a nucleolar candidate tumor suppressor gene in cervical cancer that modulates the angiogenic phenotype. Oncogene. 2006;25:588-98 pubmed
blocking or activating experiments; mouse	Gomez Duran A, Mulero Navarro S, Chang X, Fernandez Salguero P. LTBP-1 blockade in dioxin receptor-null mouse embryo fibroblasts decreases TGF-beta activity: Role of extracellular proteases plasmin and elastase. J Cell Biochem. 2006;97:380-92 pubmed
western blot; human	Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, et al. Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch. Am J Pathol. 2005;166:1883-94 pubmed
	Jacquemin G, Wurmser A, Huyghe M, Sun W, Homayed Z, Merle C, et al. Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. elife. 2022;11: pubmed publisher
	Ernens I, Bousquenaud M, Lenoir B, Devaux Y, Wagner D. Adenosine stimulates angiogenesis by up-regulating production of thrombospondin-1 by macrophages. J Leukoc Biol. 2015;97:9-18 pubmed publisher
	Emonard H, Bellon G, Troeberg L, Berton A, Robinet A, Henriet P, et al. Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism. J Biol Chem. 2004;279:54944-51 pubmed
	Doyen V, Rubio M, Braun D, Nakajima T, Abe J, Saito H, et al. Thrombospondin 1 is an autocrine negative regulator of human dendritic cell activation. J Exp Med. 2003;198:1277-83 pubmed
	Bocci G, Francia G, Man S, Lawler J, Kerbel R. Thrombospondin 1, a mediator of the antiangiogenic effects of low-dose metronomic chemotherapy. Proc Natl Acad Sci U S A. 2003;100:12917-22 pubmed
	Rofstad E, Henriksen K, Galappathi K, Mathiesen B. Antiangiogenic treatment with thrombospondin-1 enhances primary tumor radiation response and prevents growth of dormant pulmonary micrometastases after curative radiation therapy in human melanoma xenografts. Cancer Res. 2003;63:4055-61 pubmed
	Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt Clermont P, Kovacic H. Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol Cell Biol. 2003;23:5401-8 pubmed
	Rhodes K, Moon L, Fawcett J. Inhibiting cell proliferation during formation of the glial scar: effects on axon regeneration in the CNS. Neuroscience. 2003;120:41-56 pubmed
	Sakai K, Sumi Y, Muramatsu H, Hata K, Muramatsu T, Ueda M. Thrombospondin-1 promotes fibroblast-mediated collagen gel contraction caused by activation of latent transforming growth factor beta-1. J Dermatol Sci. 2003;31:99-109 pubmed
	Siegel Axel D, Runge H, Seipel L, Riessen R. Effects of cerivastatin on human arterial smooth muscle cell growth and extracellular matrix expression at varying glucose and low-density lipoprotein levels. J Cardiovasc Pharmacol. 2003;41:422-33 pubmed
	Volpert O, Pili R, Sikder H, Nelius T, Zaichuk T, Morris C, et al. Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1. Cancer Cell. 2002;2:473-83 pubmed
	Goel M, Diamond S. Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma. Blood. 2002;100:3797-803 pubmed
	Rofstad E, Graff B. Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts. J Invest Dermatol. 2001;117:1042-9 pubmed
	Tenan M, Fulci G, Albertoni M, Diserens A, Hamou M, El Atifi Borel M, et al. Thrombospondin-1 is downregulated by anoxia and suppresses tumorigenicity of human glioblastoma cells. J Exp Med. 2000;191:1789-98 pubmed
	Shi S, Cote R, Hawes D, Thu S, Shi Y, Young L, et al. Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?. J Histochem Cytochem. 1999;47:463-70 pubmed
	Gupta K, Gupta P, Solovey A, Hebbel R. Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin. Biochim Biophys Acta. 1999;1453:63-73 pubmed

product information

Product Type :

Antibody

Product Name :

Thrombospondin 1 Monoclonal Antibody (A4.1)

Catalog # :

MA5-13377

Quantity :

500 µL

Price :

US 496

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Mouse

Reactivity :

Bovine, Chicken, Human, Mouse, Rat

Applications :

Flow Cytometry: Assay Dependent, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Bovine, Chicken, Human, Mouse, Rat

Clone :

A4.1

Isotype :

IgM

Storage :

4° C

Description :

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Immunogen :

Calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay Dependent, Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

Glycoprotein G; tbsp1; THBS; THBS1; THBS-1; thrombospondin; thrombospondin 1; Thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1; TSP; TSP1; TSP-1