This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
EGFR Monoclonal Antibody (199.12)
catalog :
MA5-13319
quantity :
500 µL
price :
US 496
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
199.12
reactivity :
African green monkey, human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, blocking or activating experiments
citations: 66
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:100; loading ...; fig 6s1a
Lyashenko E, Niepel M, Dixit P, Lim S, Sorger P, Vitkup D. Receptor-based mechanism of relative sensing and cell memory in mammalian signaling networks. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; fig s5a
Kadlecova Z, Spielman S, Loerke D, Mohanakrishnan A, Reed D, Schmid S. Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2. J Cell Biol. 2017;216:167-179 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig s6
Truong D, Puleo J, Llave A, Mouneimne G, Kamm R, Nikkhah M. Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment. Sci Rep. 2016;6:34094 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 1c
Elkin S, Oswald N, Reed D, Mettlen M, Macmillan J, Schmid S. Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis. Traffic. 2016;17:1139-49 pubmed publisher
  • immunocytochemistry; human; 1:1000
Zhou J, Joshi B, Duan X, Pant A, Qiu Z, Kuick R, et al. EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging. Clin Transl Gastroenterol. 2015;6:e101 pubmed publisher
  • immunoprecipitation; human; 0.8 ug/time
  • western blot; human
Hasegawa Y, Takahashi M, Ariki S, Asakawa D, Tajiri M, Wada Y, et al. Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling. Oncogene. 2015;34:838-45 pubmed publisher
  • chromatin immunoprecipitation; human
Chou C, Lee H, Tsou P, Chen C, Hsu J, Yamaguchi H, et al. mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes. Sci Signal. 2014;7:rs1 pubmed publisher
  • blocking or activating experiments; human
Otero C, Linke M, Sanchez P, Gonzalez A, Schaap I. Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization. PLoS ONE. 2013;8:e83086 pubmed publisher
  • immunocytochemistry; human
Henriksen L, Grandal M, Knudsen S, van Deurs B, Grøvdal L. Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands. PLoS ONE. 2013;8:e58148 pubmed publisher
  • chromatin immunoprecipitation; human
Han W, Carpenter R, Cao X, Lo H. STAT1 gene expression is enhanced by nuclear EGFR and HER2 via cooperation with STAT3. Mol Carcinog. 2013;52:959-69 pubmed publisher
  • ELISA; human; 0.3 ug/ml
Schwenk J, Poetz O, Zeillinger R, Joos T. A Miniaturized Ligand Binding Assay for EGFR. Int J Proteomics. 2012;2012:247059 pubmed publisher
  • immunoprecipitation; human; 1.5 ug
Siegemund M, Pollak N, Seifert O, Wahl K, Hanak K, Vogel A, et al. Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity. Cell Death Dis. 2012;3:e295 pubmed publisher
  • immunocytochemistry; human
Rong G, Reinhard B. Monitoring the size and lateral dynamics of ErbB1 enriched membrane domains through live cell plasmon coupling microscopy. PLoS ONE. 2012;7:e34175 pubmed publisher
  • flow cytometry; human
  • immunoprecipitation; human
  • immunocytochemistry; human
Grandal M, Grøvdal L, Henriksen L, Andersen M, Holst M, Madshus I, et al. Differential roles of Grb2 and AP-2 in p38 MAPK- and EGF-induced EGFR internalization. Traffic. 2012;13:576-85 pubmed publisher
  • blocking or activating experiments; human; 100 ug/ml
Pötz O, Schneiderhan Marra N, Henzler T, Herget T, Joos T. Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays. Methods Mol Biol. 2012;795:191-202 pubmed publisher
  • immunoprecipitation; African green monkey
  • western blot; African green monkey
Yang X, Guo Z, Sun F, Li W, Alfano A, Shimelis H, et al. Novel membrane-associated androgen receptor splice variant potentiates proliferative and survival responses in prostate cancer cells. J Biol Chem. 2011;286:36152-60 pubmed publisher
  • western blot; human
Deb T, Zuo A, Wang Y, Barndt R, Cheema A, Sengupta S, et al. Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex. Am J Physiol Cell Physiol. 2011;300:C1139-54 pubmed publisher
  • immunoprecipitation; rat; 2 ug/ml
Poindexter N, Williams R, Powis G, Jen E, Caudle A, Chada S, et al. IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes. Exp Dermatol. 2010;19:714-22 pubmed publisher
  • western blot; human
Chou C, Jing N, Yamaguchi H, Tsou P, Lee H, Chen C, et al. High speed digital protein interaction analysis using microfluidic single molecule detection system. Lab Chip. 2010;10:1793-8 pubmed publisher
  • immunocytochemistry; human
Chung I, Akita R, Vandlen R, Toomre D, Schlessinger J, Mellman I. Spatial control of EGF receptor activation by reversible dimerization on living cells. Nature. 2010;464:783-7 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
Hsu S, Miller S, Wang Y, Hung M. Nuclear EGFR is required for cisplatin resistance and DNA repair. Am J Transl Res. 2009;1:249-58 pubmed
  • western blot; human
Zhang Y, Su H, Rahimi M, Tochihara R, Tang C. EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer. Int J Cancer. 2009;125:2021-8 pubmed publisher
  • immunoprecipitation; human; fig 6
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 6
Roepstorff K, Grandal M, Henriksen L, Knudsen S, Lerdrup M, Grøvdal L, et al. Differential effects of EGFR ligands on endocytic sorting of the receptor. Traffic. 2009;10:1115-27 pubmed publisher
  • immunoprecipitation; human
Bitler B, Menzl I, Huerta C, Sands B, Knowlton W, Chang A, et al. Intracellular MUC1 peptides inhibit cancer progression. Clin Cancer Res. 2009;15:100-9 pubmed publisher
  • western blot; human; 1:500
Klessner J, Desai B, Amargo E, Getsios S, Green K. EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2. Mol Biol Cell. 2009;20:328-37 pubmed publisher
  • immunocytochemistry; human
Yoon H, Lee J, Randazzo P. ARAP1 regulates endocytosis of EGFR. Traffic. 2008;9:2236-52 pubmed publisher
  • immunoprecipitation; human; 1:50
  • immunohistochemistry; human; 1:500
  • western blot; human; 1:500
Rajagopalan V, Zucker I, Jones J, Carlson M, MA Y. Cardiac ErbB-1/ErbB-2 mutant expression in young adult mice leads to cardiac dysfunction. Am J Physiol Heart Circ Physiol. 2008;295:H543-54 pubmed publisher
  • immunoprecipitation; human
Umebayashi K, Stenmark H, Yoshimori T. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 2008;19:3454-62 pubmed publisher
  • immunoprecipitation; African green monkey
Xu W, Soga S, Beebe K, Lee M, Kim Y, Trepel J, et al. Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition. Br J Cancer. 2007;97:741-4 pubmed
  • immunoprecipitation; human
  • western blot; human
Yamasaki F, Zhang D, Bartholomeusz C, Sudo T, Hortobagyi G, Kurisu K, et al. Sensitivity of breast cancer cells to erlotinib depends on cyclin-dependent kinase 2 activity. Mol Cancer Ther. 2007;6:2168-77 pubmed
  • immunoprecipitation; human; 2 ug/time
Yamasaki F, Johansen M, Zhang D, Krishnamurthy S, Felix E, Bartholomeusz C, et al. Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt. Cancer Res. 2007;67:5779-88 pubmed
  • immunoprecipitation; human
  • western blot; human
Faivre E, Lange C. Progesterone receptors upregulate Wnt-1 to induce epidermal growth factor receptor transactivation and c-Src-dependent sustained activation of Erk1/2 mitogen-activated protein kinase in breast cancer cells. Mol Cell Biol. 2007;27:466-80 pubmed
  • immunoprecipitation; mouse
Crotty T, Cai J, Sakane F, Taketomi A, Prescott S, Topham M. Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling. Proc Natl Acad Sci U S A. 2006;103:15485-90 pubmed
  • immunoprecipitation; human
Martin N, Mohney R, Dunn S, Das M, Scappini E, O Bryan J. Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling. Mol Pharmacol. 2006;70:1643-53 pubmed
  • immunocytochemistry; human; 1:500
Funes M, Miller J, Lai C, Carraway K, Sweeney C. The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3. J Biol Chem. 2006;281:19310-9 pubmed
  • immunoprecipitation; human
Lo H, Ali Seyed M, Wu Y, Bartholomeusz G, Hsu S, Hung M. Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1. J Cell Biochem. 2006;98:1570-83 pubmed
  • flow cytometry; human; 10 ug/ml
Kim Y, Bhandari R, Cochran J, Kuriyan J, Wittrup K. Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast. Proteins. 2006;62:1026-35 pubmed
  • flow cytometry; mouse; 10 ug/ml
Whitson K, Whitson S, Red Brewer M, McCoy A, Vitali A, Walker F, et al. Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor. Biochemistry. 2005;44:14920-31 pubmed
  • western blot; human
Pangburn H, Kraus H, Ahnen D, Rice P. Sulindac metabolites inhibit epidermal growth factor receptor activation and expression. J Carcinog. 2005;4:16 pubmed
  • immunoprecipitation; human
  • western blot; human
Xia W, Gerard C, Liu L, Baudson N, Ory T, Spector N. Combining lapatinib (GW572016), a small molecule inhibitor of ErbB1 and ErbB2 tyrosine kinases, with therapeutic anti-ErbB2 antibodies enhances apoptosis of ErbB2-overexpressing breast cancer cells. Oncogene. 2005;24:6213-21 pubmed
  • immunoprecipitation; African green monkey
Xu W, Yuan X, Xiang Z, Mimnaugh E, Marcu M, Neckers L. Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex. Nat Struct Mol Biol. 2005;12:120-6 pubmed
  • western blot; human; fig 7
Shen X, Kramer R. Adhesion-mediated squamous cell carcinoma survival through ligand-independent activation of epidermal growth factor receptor. Am J Pathol. 2004;165:1315-29 pubmed
Choi J, Kang C, Park J. EGFR-targeted fluorescent imaging using the da Vinci® Firefly™ camera for gallbladder cancer. World J Surg Oncol. 2022;20:201 pubmed publisher
Acevedo D xed az A, Morales Cab xe1 n B, Zayas Santiago A, Mart xed nez Montemayor M, Su xe1 rez Arroyo I. SCAMP3 Regulates EGFR and Promotes Proliferation and Migration of Triple-Negative Breast Cancer Cells through the Modulation of AKT, ERK, and STAT3 Signaling Pathways. Cancers (Basel). 2022;14: pubmed publisher
Troitskaya O, Novak D, Nushtaeva A, Savinkova M, Varlamov M, Ermakov M, et al. EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor. Int J Mol Sci. 2021;22: pubmed publisher
Gerosa L, Chidley C, Fröhlich F, Sanchez G, Lim S, Muhlich J, et al. Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells. Cell Syst. 2020;11:478-494.e9 pubmed publisher
Dixit P, Lyashenko E, Niepel M, Vitkup D. Maximum Entropy Framework for Predictive Inference of Cell Population Heterogeneity and Responses in Signaling Networks. Cell Syst. 2020;10:204-212.e8 pubmed publisher
Kaur H, Sparvoli D, Osakada H, Iwamoto M, Haraguchi T, Turkewitz A. An endosomal syntaxin and the AP-3 complex are required for formation and maturation of candidate lysosome-related secretory organelles (mucocysts) in Tetrahymena thermophila. Mol Biol Cell. 2017;28:1551-1564 pubmed publisher
Pendharkar S, Mattoo S, Grover S. Sexual dysfunctions in alcohol-dependent men: A study from north India. Indian J Med Res. 2016;144:393-399 pubmed publisher
Zelenko Z, Gallagher E, Tobin Hess A, Belardi V, Rostoker R, Blank J, et al. Silencing vimentin expression decreases pulmonary metastases in a pre-diabetic mouse model of mammary tumor progression. Oncogene. 2017;36:1394-1403 pubmed publisher
Zhu H, Cao X, Ali Osman F, Keir S, Lo H. EGFR and EGFRvIII interact with PUMA to inhibit mitochondrial translocalization of PUMA and PUMA-mediated apoptosis independent of EGFR kinase activity. Cancer Lett. 2010;294:101-10 pubmed publisher
Schmidt M, Hoeller D, Yu J, Furnari F, Cavenee W, Dikic I, et al. Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex. Mol Cell Biol. 2004;24:8981-93 pubmed
Fan Y, Wong L, Deb T, Johnson G. Ligand regulates epidermal growth factor receptor kinase specificity: activation increases preference for GAB1 and SHC versus autophosphorylation sites. J Biol Chem. 2004;279:38143-50 pubmed
Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
Barzilay E, Ben Califa N, Supino Rosin L, Kashman Y, Hirschberg K, Elazar Z, et al. Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity. J Biol Chem. 2004;279:6847-52 pubmed
Nielsen U, Cardone M, Sinskey A, MacBeath G, Sorger P. Profiling receptor tyrosine kinase activation by using Ab microarrays. Proc Natl Acad Sci U S A. 2003;100:9330-5 pubmed
Schmidt M, Furnari F, Cavenee W, Bogler O. Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization. Proc Natl Acad Sci U S A. 2003;100:6505-10 pubmed
Cordero J, Cozzolino M, Lu Y, Vidal M, Slatopolsky E, Stahl P, et al. 1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor. J Biol Chem. 2002;277:38965-71 pubmed
Stoll S, Kansra S, Elder J. Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture. J Biol Chem. 2002;277:26839-45 pubmed
Alper O -, Bergmann Leitner E, Bennett T, Hacker N, Stromberg K, Stetler Stevenson W. Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells. J Natl Cancer Inst. 2001;93:1375-84 pubmed
Gaudry C, Palka H, Dusek R, Huen A, Khandekar M, Hudson L, et al. Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation. J Biol Chem. 2001;276:24871-80 pubmed
Tikhomirov O, Carpenter G. Geldanamycin induces ErbB-2 degradation by proteolytic fragmentation. J Biol Chem. 2000;275:26625-31 pubmed
Tang C, Gong X, Moscatello D, Wong A, Lippman M. Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer. Cancer Res. 2000;60:3081-7 pubmed
Supino Rosin L, Yoshimura A, Yarden Y, Elazar Z, Neumann D. Intracellular retention and degradation of the epidermal growth factor receptor, two distinct processes mediated by benzoquinone ansamycins. J Biol Chem. 2000;275:21850-5 pubmed
Supino Rosin L, Yoshimura A, Altaratz H, Neumann D. A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation. Eur J Biochem. 1999;263:410-9 pubmed
Baron A, Lafky J, Connolly D, Peoples J, O Kane D, Suman V, et al. A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera. J Immunol Methods. 1998;219:23-43 pubmed
product information
Product Type :
Antibody
Product Name :
EGFR Monoclonal Antibody (199.12)
Catalog # :
MA5-13319
Quantity :
500 µL
Price :
US 496
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human
Applications :
Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL
Species :
Human
Clone :
199.12
Isotype :
IgG2a
Storage :
4° C
Description :
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Immunogen :
Extracellular domain of human recombinant EGFR protein
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL
Aliases :
2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA