EGFR Monoclonal Antibody (111.6), Biotin | Invitrogen MA5-13266 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Monoclonal Antibody (111.6), Biotin

catalog :

MA5-13266

quantity :

500 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

111.6

reactivity :

human, mouse, dogs, domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 49

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; fig 3a	Kang S, Wang Y, Reder N, Liu J. Multiplexed Molecular Imaging of Biomarker-Targeted SERS Nanoparticles on Fresh Tissue Specimens with Channel-Compressed Spectrometry. PLoS ONE. 2016;11:e0163473 pubmed publisher
immunocytochemistry; human immunohistochemistry; human	Fichter C, Timme S, Braun J, Gudernatsch V, Schöpflin A, Bogatyreva L, et al. EGFR, HER2 and HER3 dimerization patterns guide targeted inhibition in two histotypes of esophageal cancer. Int J Cancer. 2014;135:1517-30 pubmed publisher
immunohistochemistry; human	Terragni R, Casadei Gardini A, Sabattini S, Bettini G, Amadori D, Talamonti C, et al. EGFR, HER-2 and KRAS in canine gastric epithelial tumors: a potential human model?. PLoS ONE. 2014;9:e85388 pubmed publisher
immunohistochemistry; dogs; 1:100	Danne C, Dubrac S, Trieu Cuot P, Dramsi S. Single cell stochastic regulation of pilus phase variation by an attenuation-like mechanism. PLoS Pathog. 2014;10:e1003860 pubmed publisher
immunohistochemistry; human; 1:100	Böger C, Kalthoff H, Goodman S, Behrens H, Rocken C. Integrins and their ligands are expressed in non-small cell lung cancer but not correlated with parameters of disease progression. Virchows Arch. 2014;464:69-78 pubmed publisher
immunohistochemistry; human	Keren S, Shoude Z, Lu Z, Beibei Y. Role of EGFR as a prognostic factor for survival in head and neck cancer: a meta-analysis. Tumour Biol. 2014;35:2285-95 pubmed
immunohistochemistry; human	Foda A. No-cost manual method for preparation of tissue microarrays having high quality comparable to semiautomated methods. Appl Immunohistochem Mol Morphol. 2013;21:271-4 pubmed publisher
blocking or activating experiments; human; 1,000 ug/ml	Pötz O, Schneiderhan Marra N, Henzler T, Herget T, Joos T. Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays. Methods Mol Biol. 2012;795:191-202 pubmed publisher
immunohistochemistry; human; 1:50	Munirah M, Siti Aishah M, Reena M, Sharifah N, Rohaizak M, Norlia A, et al. Identification of different subtypes of breast cancer using tissue microarray. Rom J Morphol Embryol. 2011;52:669-77 pubmed
immunohistochemistry; human	Soonthornthum T, Arias Pulido H, Joste N, Lomo L, Muller C, Rutledge T, et al. Epidermal growth factor receptor as a biomarker for cervical cancer. Ann Oncol. 2011;22:2166-78 pubmed publisher
immunohistochemistry; human	Soda Y, Marumoto T, Friedmann Morvinski D, Soda M, Liu F, Michiue H, et al. Transdifferentiation of glioblastoma cells into vascular endothelial cells. Proc Natl Acad Sci U S A. 2011;108:4274-80 pubmed publisher
immunohistochemistry; human; 1:200	Vollebergh M, Lips E, Nederlof P, Wessels L, Schmidt M, van Beers E, et al. An aCGH classifier derived from BRCA1-mutated breast cancer and benefit of high-dose platinum-based chemotherapy in HER2-negative breast cancer patients. Ann Oncol. 2011;22:1561-70 pubmed publisher
immunohistochemistry; human; 1:100	Brunner A, Grimm C, Polterauer S, Hefler L, Stani J, Dudek G, et al. Expression of epidermal growth factor receptor and vascular endothelial growth factor in vaginal squamous cell cancer. Am J Obstet Gynecol. 2011;204:171.e1-6 pubmed publisher
flow cytometry; human	Tarcic G, Yarden Y. MAP Kinase activation by receptor tyrosine kinases: in control of cell migration. Methods Mol Biol. 2010;661:125-35 pubmed publisher
immunohistochemistry; human; 1:25	Mathieu A, Weynand B, Verbeken E, da Silva S, Decaestecker C, Salmon I, et al. Comparison of four antibodies for immunohistochemical evaluation of epidermal growth factor receptor expression in non-small cell lung cancer. Lung Cancer. 2010;69:46-50 pubmed publisher
immunocytochemistry; human; 4 ug/ml	Gruber H, Hoelscher G, Loeffler B, Chow Y, Ingram J, Halligan W, et al. Prostaglandin E1 and misoprostol increase epidermal growth factor production in 3D-cultured human annulus cells. Spine J. 2009;9:760-6 pubmed publisher
immunocytochemistry; human	Xu Y, Shao Y, Zhou J, Voorhees J, Fisher G. Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes. J Cell Biochem. 2009;107:873-80 pubmed publisher
immunohistochemistry; human	Dogu G, Ozkan M, Ozturk F, Dikilitas M, Er O, Ozturk A. Triple-negative breast cancer: immunohistochemical correlation with basaloid markers and prognostic value of survivin. Med Oncol. 2010;27:34-9 pubmed publisher
immunoprecipitation; human	Lowe A, Ozer B, Wiepz G, Bertics P, Abbott N. Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: epidermal growth factor receptor as a model system. Lab Chip. 2008;8:1357-64 pubmed publisher
immunohistochemistry; human	Yoshida A, Kanno H, Watabe D, Akasaka T, Sawai T. The role of heparin-binding EGF-like growth factor and amphiregulin in the epidermal proliferation of psoriasis in cooperation with TNFalpha. Arch Dermatol Res. 2008;300:37-45 pubmed
immunohistochemistry; human; 1:100	Kreike B, van Kouwenhove M, Horlings H, Weigelt B, Peterse H, Bartelink H, et al. Gene expression profiling and histopathological characterization of triple-negative/basal-like breast carcinomas. Breast Cancer Res. 2007;9:R65 pubmed
immunocytochemistry; human	Carlson A, Coghlan L, Gillenwater A, Richards Kortum R. Dual-mode reflectance and fluorescence near-video-rate confocal microscope for architectural, morphological and molecular imaging of tissue. J Microsc. 2007;228:11-24 pubmed
immunohistochemistry; human	Hatiboglu M, Cosar M, Iplikcioglu A, Ozcan D. Sex steroid and epidermal growth factor profile of giant meningiomas associated with pregnancy. Surg Neurol. 2008;69:356-62; discussion 362-3 pubmed
immunohistochemistry; human; 1:50	Marci V, Volante M, Cappia S, Righi L, Novello C, Scagliotti G, et al. Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma. Virchows Arch. 2007;451:729-36 pubmed
immunohistochemistry; domestic sheep	Zhou P, Liu D, Cang M, Ma Y, Yang D, Li H, et al. TGFalpha and EGFR in ovine preimplantation embryos and effects on development. Anim Reprod Sci. 2008;104:370-81 pubmed
immunohistochemistry; human; 1:50	Tampellini M, Longo M, Cappia S, Bacillo E, Alabiso I, Volante M, et al. Co-expression of EGF receptor, TGFalpha and S6 kinase is significantly associated with colorectal carcinomas with distant metastases at diagnosis. Virchows Arch. 2007;450:321-8 pubmed
immunohistochemistry; mouse western blot; mouse	Miettinen P, Ustinov J, Ormio P, Gao R, Palgi J, Hakonen E, et al. Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth. Diabetes. 2006;55:3299-308 pubmed
immunohistochemistry; human; 1:100	Hannemann J, Kristel P, VAN Tinteren H, Bontenbal M, van Hoesel Q, Smit W, et al. Molecular subtypes of breast cancer and amplification of topoisomerase II alpha: predictive role in dose intensive adjuvant chemotherapy. Br J Cancer. 2006;95:1334-41 pubmed
immunohistochemistry; human	Doger F, Meteoglu I, Tuncyurek P, Okyay P, Cevikel H. Does the EGFR and VEGF expression predict the prognosis in colon cancer?. Eur Surg Res. 2006;38:540-4 pubmed
immunohistochemistry; human; 1:10	Hsu E, Gillenwater A, Hasan M, Williams M, El Naggar A, Richards Kortum R. Real-time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular-specific contrast agent. Int J Cancer. 2006;118:3062-71 pubmed
immunohistochemistry; human; 1 ug/ml	Watwe V, Javle M, Lawrence D, Groth J, Iyer R, El Hajjar D, et al. Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer. Am J Clin Oncol. 2005;28:560-4 pubmed
immunocytochemistry; human	Nida D, Rahman M, Carlson K, Richards Kortum R, Follen M. Fluorescent nanocrystals for use in early cervical cancer detection. Gynecol Oncol. 2005;99:S89-94 pubmed
immunohistochemistry; human; 1:100	Akayama Y, Takekida S, Ohara N, Tateiwa H, Chen W, Nakabayashi K, et al. Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum. Hum Reprod. 2005;20:2708-14 pubmed
immunoprecipitation; human	Jang C, Tingey M, Korpi N, Wiepz G, Schiller J, Bertics P, et al. Using liquid crystals to report membrane proteins captured by affinity microcontact printing from cell lysates and membrane extracts. J Am Chem Soc. 2005;127:8912-3 pubmed
immunohistochemistry; human; 1:500	Haas Kogan D, Prados M, Tihan T, Eberhard D, Jelluma N, Arvold N, et al. Epidermal growth factor receptor, protein kinase B/Akt, and glioma response to erlotinib. J Natl Cancer Inst. 2005;97:880-7 pubmed
	Riebensahm C, Joosse S, Mohme M, Hanssen A, Matschke J, Goy Y, et al. Clonality of circulating tumor cells in breast cancer brain metastasis patients. Breast Cancer Res. 2019;21:101 pubmed publisher
	Hsu E, Anslyn E, Dharmawardhane S, Alizadeh Naderi R, Aaron J, Sokolov K, et al. A far-red fluorescent contrast agent to image epidermal growth factor receptor expression. Photochem Photobiol. 2004;79:272-9 pubmed
	Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
	Gossett D, Alo P, Bristow R, Galati M, Kyshtoobayeva A, Fruehauf J, et al. Inability of immunohistochemistry to predict clinical outcomes of endometrial cancer patients. Int J Gynecol Cancer. 2004;14:145-51 pubmed
	Santoro A, Cavina R, Latteri F, Zucali P, Ginanni V, Campagnoli E, et al. Activity of a specific inhibitor, gefitinib (Iressa, ZD1839), of epidermal growth factor receptor in refractory non-small-cell lung cancer. Ann Oncol. 2004;15:33-7 pubmed
	Barzilay E, Ben Califa N, Supino Rosin L, Kashman Y, Hirschberg K, Elazar Z, et al. Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity. J Biol Chem. 2004;279:6847-52 pubmed
	Sokolov K, Aaron J, Hsu B, Nida D, Gillenwater A, Follen M, et al. Optical systems for in vivo molecular imaging of cancer. Technol Cancer Res Treat. 2003;2:491-504 pubmed
	Chen B, van den Brekel M, Buschers W, Balm A, van Velthuysen M. Validation of tissue array technology in head and neck squamous cell carcinoma. Head Neck. 2003;25:922-30 pubmed
	Nielsen U, Cardone M, Sinskey A, MacBeath G, Sorger P. Profiling receptor tyrosine kinase activation by using Ab microarrays. Proc Natl Acad Sci U S A. 2003;100:9330-5 pubmed
	Daneshmand M, Parolin D, Hirte H, Major P, Goss G, Stewart D, et al. A pharmacodynamic study of the epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 in metastatic colorectal cancer patients. Clin Cancer Res. 2003;9:2457-64 pubmed
	Piepkorn M, Predd H, Underwood R, Cook P. Proliferation-differentiation relationships in the expression of heparin-binding epidermal growth factor-related factors and erbB receptors by normal and psoriatic human keratinocytes. Arch Dermatol Res. 2003;295:93-101 pubmed
	Planque S, Taguchi H, Burr G, Bhatia G, Karle S, Zhou Y, et al. Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity. J Biol Chem. 2003;278:20436-43 pubmed
	Praetorius L, Krogh J, Horn T. Retroperitoneal cystic papillary carcinoma of probable nephrogenic origin. Scand J Urol Nephrol. 2001;35:147-9 pubmed
	Baron A, Lafky J, Connolly D, Peoples J, O Kane D, Suman V, et al. A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera. J Immunol Methods. 1998;219:23-43 pubmed

product information

Product Type :

Antibody

Product Name :

EGFR Monoclonal Antibody (111.6), Biotin

Catalog # :

MA5-13266

Quantity :

500 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL

Species :

Human

Clone :

111.6

Isotype :

IgG1

Storage :

4° C

Description :

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

Extracellular domain of human recombinant EGFR protein

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2