This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
DNA-PK Monoclonal Antibody (18-2)
catalog :
MA5-13238
quantity :
500 µL
price :
US 480
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
18-2
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments
citations: 57
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human; 1:2000; fig 7
He S, Zhao Z, Yang Y, O Connell D, Zhang X, Oh S, et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015;6:7839 pubmed publisher
  • western blot; human; 1:200; fig 4
Ochi T, Blackford A, Coates J, Jhujh S, Mehmood S, Tamura N, et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015;347:185-188 pubmed publisher
  • western blot; human; 1:200; tbl 2
Britton S, Coates J, Jackson S. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol. 2013;202:579-95 pubmed publisher
  • immunocytochemistry; human; 1:150
Javvadi P, Makino H, Das A, Lin Y, Chen D, Chen B, et al. Threonine 2609 phosphorylation of the DNA-dependent protein kinase is a critical prerequisite for epidermal growth factor receptor-mediated radiation resistance. Mol Cancer Res. 2012;10:1359-68 pubmed publisher
  • western blot; human
Mauro M, Rego M, Boisvert R, Esashi F, Cavallo F, Jasin M, et al. p21 promotes error-free replication-coupled DNA double-strand break repair. Nucleic Acids Res. 2012;40:8348-60 pubmed
  • western blot; human
Kang K, Zhu C, Wong Y, Gao Q, Ty A, Wong M. Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair. Int J Radiat Oncol Biol Phys. 2012;83:e43-52 pubmed publisher
  • immunoprecipitation; rat
  • western blot; rat; 1:400
Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, De Stefano M, et al. Sublethal doses of ?-amyloid peptide abrogate DNA-dependent protein kinase activity. J Biol Chem. 2012;287:2618-31 pubmed publisher
  • western blot; human
Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res. 2011;39:9605-19 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, et al. A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia. J Cell Sci. 2011;124:1943-51 pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:100
Ruiz Herrera A, Smirnova A, Khoriauli L, Nergadze S, Mondello C, Giulotto E. Gene amplification in human cells knocked down for RAD54. Genome Integr. 2011;2:5 pubmed publisher
  • blocking or activating experiments; human; 200 ug/ml
Choi Y, Nash K, Byrne B, Muzyczka N, Song S. The effect of DNA-dependent protein kinase on adeno-associated virus replication. PLoS ONE. 2010;5:e15073 pubmed publisher
  • immunocytochemistry; human
  • immunohistochemistry; mouse
  • western blot; mouse
Papeta N, Zheng Z, Schon E, Brosel S, Altintas M, Nasr S, et al. Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice. J Clin Invest. 2010;120:4055-64 pubmed publisher
  • western blot; human
Takai H, Xie Y, de Lange T, Pavletich N. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev. 2010;24:2019-30 pubmed publisher
  • western blot; human; 1:500
Yasaei H, Slijepcevic P. Defective Artemis causes mild telomere dysfunction. Genome Integr. 2010;1:3 pubmed publisher
  • western blot; human
Yu X, Wang H, Wang P, Chen B, Wang Y. The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival. J Cell Physiol. 2011;226:369-74 pubmed publisher
  • western blot; human
Velkova A, Carvalho M, Johnson J, Tavtigian S, Monteiro A. Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle. 2010;9:1421-33 pubmed
  • western blot; human
Choul Li S, Drobecq H, Aumercier M. DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms. Biochem Biophys Res Commun. 2009;390:839-44 pubmed publisher
  • western blot; human
Britton S, Frit P, Biard D, Salles B, Calsou P. ARTEMIS nuclease facilitates apoptotic chromatin cleavage. Cancer Res. 2009;69:8120-6 pubmed publisher
  • immunoprecipitation; human; 1:5000
Daya S, Cortez N, Berns K. Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway. J Virol. 2009;83:11655-64 pubmed publisher
  • western blot; mouse
Meulle A, Salles B, Daviaud D, Valet P, Muller C. Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis. PLoS ONE. 2008;3:e3345 pubmed publisher
  • chromatin immunoprecipitation; human
Schild Poulter C, Su A, Shih A, Kelly O, Fritzler M, Goldstein R, et al. Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases. Rheumatology (Oxford). 2008;47:165-71 pubmed publisher
  • western blot; human
Wu P, Frit P, Malivert L, Revy P, Biard D, Salles B, et al. Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell. J Biol Chem. 2007;282:31937-43 pubmed
  • western blot; human
Hegarat N, Cardoso G, Rusconi F, Francois J, Praseuth D. Analytical biochemistry of DNA--protein assemblies from crude cell extracts. Nucleic Acids Res. 2007;35:e92 pubmed
  • immunocytochemistry; human
  • western blot; human
Despras E, Pfeiffer P, Salles B, Calsou P, Kuhfittig Kulle S, Angulo J, et al. Long-term XPC silencing reduces DNA double-strand break repair. Cancer Res. 2007;67:2526-34 pubmed
  • western blot; mouse
Kiefer K, Oshinsky J, Kim J, Nakajima P, Bosma G, Bosma M. The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination. Proc Natl Acad Sci U S A. 2007;104:2843-8 pubmed
  • western blot; human; 1 ug/ml
Shackelford D. DNA end joining activity is reduced in Alzheimer's disease. Neurobiol Aging. 2006;27:596-605 pubmed
Chen W, Chiang J, Shang Z, Palchik G, Newman C, Zhang Y, et al. DNA-PKcs and ATM modulate mitochondrial ADP-ATP exchange as an oxidative stress checkpoint mechanism. EMBO J. 2023;42:e112094 pubmed publisher
Cisneros Aguirre M, Lopezcolorado F, Tsai L, Bhargava R, Stark J. The importance of DNAPKcs for blunt DNA end joining is magnified when XLF is weakened. Nat Commun. 2022;13:3662 pubmed publisher
Guo G, Gao M, Gao X, Zhu B, Huang J, Tu X, et al. Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling. Nat Commun. 2021;12:2187 pubmed publisher
Guo Z, Wang S, Xie Y, Han Y, Hu S, Guan H, et al. HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response. Cell Death Dis. 2020;11:400 pubmed publisher
Han Y, Jin F, Xie Y, Liu Y, Hu S, Liu X, et al. DNA‑PKcs PARylation regulates DNA‑PK kinase activity in the DNA damage response. Mol Med Rep. 2019;20:3609-3616 pubmed publisher
Aguila B, Morris A, Spina R, Bar E, Schraner J, Vinkler R, et al. The Ig superfamily protein PTGFRN coordinates survival signaling in glioblastoma multiforme. Cancer Lett. 2019;462:33-42 pubmed publisher
Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, et al. An in vitro model of murine middle ear epithelium. Dis Model Mech. 2016;9:1405-1417 pubmed
Adelmant G, Calkins A, Garg B, Card J, Askenazi M, Miron A, et al. DNA ends alter the molecular composition and localization of Ku multicomponent complexes. Mol Cell Proteomics. 2012;11:411-21 pubmed publisher
Sand Dejmek J, Adelmant G, Sobhian B, Calkins A, Marto J, Iglehart D, et al. Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin. Mol Cancer. 2011;10:74 pubmed publisher
He F, Li L, Kim D, Wen B, Deng X, Gutin P, et al. Adenovirus-mediated expression of a dominant negative Ku70 fragment radiosensitizes human tumor cells under aerobic and hypoxic conditions. Cancer Res. 2007;67:634-42 pubmed
Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L. Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies. Exp Mol Pathol. 2004;77:1-6 pubmed
Perrault R, Wang H, Wang M, Rosidi B, Iliakis G. Backup pathways of NHEJ are suppressed by DNA-PK. J Cell Biochem. 2004;92:781-94 pubmed
Loong S, Korzh S, Price A. Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis. Oncogene. 2004;23:5562-6 pubmed
Sawchuk D, Mansilla Soto J, Alarcon C, Singha N, Langen H, Bianchi M, et al. Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule. J Biol Chem. 2004;279:29821-31 pubmed
Repasky J, Corbett E, Boboila C, Schatz D. Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination. J Immunol. 2004;172:5478-88 pubmed
Song S, Lu Y, Choi Y, Han Y, Tang Q, Zhao G, et al. DNA-dependent PK inhibits adeno-associated virus DNA integration. Proc Natl Acad Sci U S A. 2004;101:2112-6 pubmed
Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay J. The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination. J Exp Med. 2004;199:315-21 pubmed
Onclercq Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor Gueret M. Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing. Nucleic Acids Res. 2003;31:6272-82 pubmed
Wang H, Perrault A, Takeda Y, Qin W, Wang H, Iliakis G. Biochemical evidence for Ku-independent backup pathways of NHEJ. Nucleic Acids Res. 2003;31:5377-88 pubmed
Udayakumar D, Bladen C, Hudson F, Dynan W. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003;278:41631-5 pubmed
Friesland S, Kanter Lewensohn L, Tell R, Munck Wikland E, Lewensohn R, Nilsson A. Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy. Head Neck. 2003;25:313-21 pubmed
Calsou P, Delteil C, Frit P, Drouet J, Salles B. Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment. J Mol Biol. 2003;326:93-103 pubmed
Lebrasseur N, Cote G, Miller T, Fielding R, Sawyer D. Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle. Am J Physiol Cell Physiol. 2003;284:C1149-55 pubmed
Cho N, Cordon Cardo C, Li G, Kim S. Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults. J Pathol. 2002;198:21-9 pubmed
Ma Y, Pannicke U, Schwarz K, Lieber M. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002;108:781-94 pubmed
Chen L, Trujillo K, Sung P, Tomkinson A. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase. J Biol Chem. 2000;275:26196-205 pubmed
Jhappan C, Yusufzai T, Anderson S, Anver M, Merlino G. The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase. Mol Cell Biol. 2000;20:4075-83 pubmed
Brown K, Lataxes T, Shangary S, Mannino J, Giardina J, Chen J, et al. Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism. J Biol Chem. 2000;275:6651-6 pubmed
Giffin W, Gong W, Schild Poulter C, Hache R. Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription. Mol Cell Biol. 1999;19:4065-78 pubmed
Shao R, Cao C, Zhang H, Kohn K, Wold M, Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999;18:1397-406 pubmed
Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver D, Alt F. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity. 1998;9:367-76 pubmed
product information
Product Type :
Antibody
Product Name :
DNA-PK Monoclonal Antibody (18-2)
Catalog # :
MA5-13238
Quantity :
500 µL
Price :
US 480
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Rat
Applications :
Immunohistochemistry (Paraffin): 1:50-1:100, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
Species :
Human, Rat
Clone :
18-2
Isotype :
IgG1
Storage :
4° C
Description :
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.
Immunogen :
Human DNA-dependent Protein Kinase purified from HeLa cells
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:50-1:100, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
Aliases :
AI326420; AU019811; DNA-dependent protein kinase catalytic subunit; DNAPDcs; DNAPK; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; DOXNPH; doxorubicin nephropathy; dxnph; hyper-radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; I79_017613; IMD26; p350; p460; Prkdc; protein kinase, DNA activated, catalytic polypeptide; protein kinase, DNA-activated, catalytic polypeptide; scid; severe combined immunodeficiency; slip; Xrcc7
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA