DNA-PK Monoclonal Antibody (18-2), Biotin | Invitrogen MA5-13235 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DNA-PK Monoclonal Antibody (18-2), Biotin

catalog :

MA5-13235

quantity :

500 µL

price :

US 456.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

18-2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 42

Published Application/Species/Sample/Dilution	Reference
western blot; human	Mauro M, Rego M, Boisvert R, Esashi F, Cavallo F, Jasin M, et al. p21 promotes error-free replication-coupled DNA double-strand break repair. Nucleic Acids Res. 2012;40:8348-60 pubmed
western blot; human	Kang K, Zhu C, Wong Y, Gao Q, Ty A, Wong M. Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair. Int J Radiat Oncol Biol Phys. 2012;83:e43-52 pubmed publisher
immunoprecipitation; rat western blot; rat; 1:400	Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, De Stefano M, et al. Sublethal doses of ?-amyloid peptide abrogate DNA-dependent protein kinase activity. J Biol Chem. 2012;287:2618-31 pubmed publisher
western blot; human	Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res. 2011;39:9605-19 pubmed publisher
immunocytochemistry; human western blot; human	Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, et al. A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia. J Cell Sci. 2011;124:1943-51 pubmed publisher
immunocytochemistry; human; 1:200 western blot; human; 1:100	Ruiz Herrera A, Smirnova A, Khoriauli L, Nergadze S, Mondello C, Giulotto E. Gene amplification in human cells knocked down for RAD54. Genome Integr. 2011;2:5 pubmed publisher
blocking or activating experiments; human; 200 ug/ml	Choi Y, Nash K, Byrne B, Muzyczka N, Song S. The effect of DNA-dependent protein kinase on adeno-associated virus replication. PLoS ONE. 2010;5:e15073 pubmed publisher
immunohistochemistry; mouse western blot; mouse immunocytochemistry; human	Papeta N, Zheng Z, Schon E, Brosel S, Altintas M, Nasr S, et al. Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice. J Clin Invest. 2010;120:4055-64 pubmed publisher
western blot; human	Takai H, Xie Y, de Lange T, Pavletich N. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev. 2010;24:2019-30 pubmed publisher
western blot; human; 1:500	Yasaei H, Slijepcevic P. Defective Artemis causes mild telomere dysfunction. Genome Integr. 2010;1:3 pubmed publisher
western blot; human	Yu X, Wang H, Wang P, Chen B, Wang Y. The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival. J Cell Physiol. 2011;226:369-74 pubmed publisher
western blot; human	Velkova A, Carvalho M, Johnson J, Tavtigian S, Monteiro A. Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle. 2010;9:1421-33 pubmed
western blot; human	Choul Li S, Drobecq H, Aumercier M. DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms. Biochem Biophys Res Commun. 2009;390:839-44 pubmed publisher
western blot; human	Britton S, Frit P, Biard D, Salles B, Calsou P. ARTEMIS nuclease facilitates apoptotic chromatin cleavage. Cancer Res. 2009;69:8120-6 pubmed publisher
immunoprecipitation; human; 1:5000	Daya S, Cortez N, Berns K. Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway. J Virol. 2009;83:11655-64 pubmed publisher
western blot; mouse	Meulle A, Salles B, Daviaud D, Valet P, Muller C. Positive regulation of DNA double strand break repair activity during differentiation of long life span cells: the example of adipogenesis. PLoS ONE. 2008;3:e3345 pubmed publisher
chromatin immunoprecipitation; human	Schild Poulter C, Su A, Shih A, Kelly O, Fritzler M, Goldstein R, et al. Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases. Rheumatology (Oxford). 2008;47:165-71 pubmed publisher
western blot; human	Wu P, Frit P, Malivert L, Revy P, Biard D, Salles B, et al. Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell. J Biol Chem. 2007;282:31937-43 pubmed
western blot; human	Hegarat N, Cardoso G, Rusconi F, Francois J, Praseuth D. Analytical biochemistry of DNA--protein assemblies from crude cell extracts. Nucleic Acids Res. 2007;35:e92 pubmed
immunocytochemistry; human western blot; human	Despras E, Pfeiffer P, Salles B, Calsou P, Kuhfittig Kulle S, Angulo J, et al. Long-term XPC silencing reduces DNA double-strand break repair. Cancer Res. 2007;67:2526-34 pubmed
western blot; mouse	Kiefer K, Oshinsky J, Kim J, Nakajima P, Bosma G, Bosma M. The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination. Proc Natl Acad Sci U S A. 2007;104:2843-8 pubmed
western blot; human; 1 ug/ml	Shackelford D. DNA end joining activity is reduced in Alzheimer's disease. Neurobiol Aging. 2006;27:596-605 pubmed
	Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L. Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies. Exp Mol Pathol. 2004;77:1-6 pubmed
	Perrault R, Wang H, Wang M, Rosidi B, Iliakis G. Backup pathways of NHEJ are suppressed by DNA-PK. J Cell Biochem. 2004;92:781-94 pubmed
	Loong S, Korzh S, Price A. Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis. Oncogene. 2004;23:5562-6 pubmed
	Sawchuk D, Mansilla Soto J, Alarcon C, Singha N, Langen H, Bianchi M, et al. Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule. J Biol Chem. 2004;279:29821-31 pubmed
	Repasky J, Corbett E, Boboila C, Schatz D. Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination. J Immunol. 2004;172:5478-88 pubmed
	Song S, Lu Y, Choi Y, Han Y, Tang Q, Zhao G, et al. DNA-dependent PK inhibits adeno-associated virus DNA integration. Proc Natl Acad Sci U S A. 2004;101:2112-6 pubmed
	Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay J. The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination. J Exp Med. 2004;199:315-21 pubmed
	Onclercq Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor Gueret M. Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing. Nucleic Acids Res. 2003;31:6272-82 pubmed
	Wang H, Perrault A, Takeda Y, Qin W, Wang H, Iliakis G. Biochemical evidence for Ku-independent backup pathways of NHEJ. Nucleic Acids Res. 2003;31:5377-88 pubmed
	Udayakumar D, Bladen C, Hudson F, Dynan W. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003;278:41631-5 pubmed
	Friesland S, Kanter Lewensohn L, Tell R, Munck Wikland E, Lewensohn R, Nilsson A. Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy. Head Neck. 2003;25:313-21 pubmed
	Lebrasseur N, Cote G, Miller T, Fielding R, Sawyer D. Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle. Am J Physiol Cell Physiol. 2003;284:C1149-55 pubmed
	Cho N, Cordon Cardo C, Li G, Kim S. Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults. J Pathol. 2002;198:21-9 pubmed
	Ma Y, Pannicke U, Schwarz K, Lieber M. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002;108:781-94 pubmed
	Chen L, Trujillo K, Sung P, Tomkinson A. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase. J Biol Chem. 2000;275:26196-205 pubmed
	Jhappan C, Yusufzai T, Anderson S, Anver M, Merlino G. The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase. Mol Cell Biol. 2000;20:4075-83 pubmed
	Brown K, Lataxes T, Shangary S, Mannino J, Giardina J, Chen J, et al. Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism. J Biol Chem. 2000;275:6651-6 pubmed
	Giffin W, Gong W, Schild Poulter C, Hache R. Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription. Mol Cell Biol. 1999;19:4065-78 pubmed
	Shao R, Cao C, Zhang H, Kohn K, Wold M, Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999;18:1397-406 pubmed
	Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver D, Alt F. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity. 1998;9:367-76 pubmed

product information

Product Type :

Antibody

Product Name :

DNA-PK Monoclonal Antibody (18-2), Biotin

Catalog # :

MA5-13235

Quantity :

500 µL

Price :

US 456.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Rat

Applications :

Immunohistochemistry (Paraffin): 1:50-1:100, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Rat

Clone :

18-2

Isotype :

IgG1

Storage :

4° C

Description :

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

Immunogen :

Human DNA-dependent Protein Kinase purified from HeLa cells

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:50-1:100, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

AI326420; AU019811; DNA-dependent protein kinase catalytic subunit; DNAPDcs; DNAPK; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; DOXNPH; doxorubicin nephropathy; dxnph; hyper-radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; I79_017613; IMD26; p350; p460; Prkdc; protein kinase, DNA activated, catalytic polypeptide; protein kinase, DNA-activated, catalytic polypeptide; scid; severe combined immunodeficiency; slip; Xrcc7