This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Ku70 Monoclonal Antibody (N3H10)
catalog :
MA5-13110
quantity :
500µL
price :
US 345
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
N3H10
reactivity :
Chinese hamsters, human, mouse, bovine, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, RNA immunoprecipitation, western blot knockout validation
citations: 53
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:500; fig 3
Ngo J, Matsuyama M, Kim C, Poventud Fuentes I, Bates A, Siedlak S, et al. Bax deficiency extends the survival of Ku70 knockout mice that develop lung and heart diseases. Cell Death Dis. 2015;6:e1706 pubmed publisher
  • western blot; mouse; loading ...; fig s4c
Chu S, Chabon J, Matovina C, Minehart J, Chen B, Zhang J, et al. Loss of H3K36 Methyltransferase SETD2 Impairs V(D)J Recombination during Lymphoid Development. iScience. 2020;23:100941 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3e
Tay L, Krishnan V, Sankar H, Chong Y, Chuang L, Tan T, et al. RUNX Poly(ADP-Ribosyl)ation and BLM Interaction Facilitate the Fanconi Anemia Pathway of DNA Repair. Cell Rep. 2018;24:1747-1755 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 5d
Feng W, Kawauchi D, Körkel Qu H, Deng H, Serger E, Sieber L, et al. Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation programme. Nat Commun. 2017;8:14758 pubmed publisher
  • RNA immunoprecipitation; human; fig 3
  • western blot; human; fig 3
Zhang Y, He Q, Hu Z, Feng Y, Fan L, Tang Z, et al. Long noncoding RNA LINP1 regulates repair of DNA double-strand breaks in triple-negative breast cancer. Nat Struct Mol Biol. 2016;23:522-30 pubmed publisher
  • western blot; human; fig 1
Levy E, Stolzenberg M, Bruneau J, Breton S, Neven B, Sauvion S, et al. LRBA deficiency with autoimmunity and early onset chronic erosive polyarthritis. Clin Immunol. 2016;168:88-93 pubmed publisher
  • immunoprecipitation; human; fig 1a
  • western blot; human; fig 1c
Bouley J, Saad L, Grall R, Schellenbauer A, Biard D, Paget V, et al. A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines. Oncotarget. 2015;6:27980-8000 pubmed publisher
  • immunoprecipitation; human; 1:5000; fig 7
He S, Zhao Z, Yang Y, O Connell D, Zhang X, Oh S, et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015;6:7839 pubmed publisher
  • western blot; bovine; fig 1
  • western blot; human; fig 1
Koike M, Yutoku Y, Koike A. Dynamic changes in subcellular localization of cattle XLF during cell cycle, and focus formation of cattle XLF at DNA damage sites immediately after irradiation. J Vet Med Sci. 2015;77:1109-14 pubmed publisher
  • western blot; human; fig 5
Hirakawa H, Fujisawa H, Masaoka A, Noguchi M, Hirayama R, Takahashi M, et al. The combination of Hsp90 inhibitor 17AAG and heavy-ion irradiation provides effective tumor control in human lung cancer cells. Cancer Med. 2015;4:426-36 pubmed publisher
  • western blot; human
Mizutani T, Ju Y, Imamichi Y, Osaki T, Yazawa T, Kawabe S, et al. C/EBP? (CCAAT/enhancer-binding protein ?) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1). Biochem J. 2014;460:459-71 pubmed publisher
  • immunocytochemistry; human; 1:400
  • western blot; human; 1:2000
Calkins A, Iglehart J, Lazaro J. DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1. Nucleic Acids Res. 2013;41:7378-86 pubmed publisher
  • western blot; mouse; fig 2a
Cousin W, Ho M, Desai R, Tham A, Chen R, Kung S, et al. Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage. PLoS ONE. 2013;8:e63528 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Le Guen T, Jullien L, Touzot F, Schertzer M, Gaillard L, Perderiset M, et al. Human RTEL1 deficiency causes Hoyeraal-Hreidarsson syndrome with short telomeres and genome instability. Hum Mol Genet. 2013;22:3239-49 pubmed publisher
  • western blot; human
Cottarel J, Frit P, Bombarde O, Salles B, Négrel A, Bernard S, et al. A noncatalytic function of the ligation complex during nonhomologous end joining. J Cell Biol. 2013;200:173-86 pubmed publisher
  • western blot; human
Datta J, Ghoshal K, Motiwala T, Jacob S. Novel Insights into the Molecular Mechanism of Action of DNA Hypomethylating Agents: Role of Protein Kinase C ? in Decitabine-Induced Degradation of DNA Methyltransferase 1. Genes Cancer. 2012;3:71-81 pubmed publisher
  • western blot; human; 1:1000
Dean J, McClendon A, Knudsen E. Modification of the DNA damage response by therapeutic CDK4/6 inhibition. J Biol Chem. 2012;287:29075-87 pubmed publisher
  • western blot; human
Urano M, Li G, He F, Minami A, Burgman P, Ling C. The effect of DN (dominant-negative) Ku70 and reoxygenation on hypoxia cell-kill: evidence of hypoxia-induced potentially lethal damage. Int J Radiat Biol. 2012;88:515-22 pubmed publisher
  • western blot; human
Fell V, Schild Poulter C. Ku regulates signaling to DNA damage response pathways through the Ku70 von Willebrand A domain. Mol Cell Biol. 2012;32:76-87 pubmed publisher
  • western blot; human; fig 2
Sand Dejmek J, Adelmant G, Sobhian B, Calkins A, Marto J, Iglehart D, et al. Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin. Mol Cancer. 2011;10:74 pubmed publisher
  • western blot; human; tbl 1
Martinez Marignac V, Rodrigue A, Davidson D, Couillard M, Al Moustafa A, Abramovitz M, et al. The effect of a DNA repair gene on cellular invasiveness: XRCC3 over-expression in breast cancer cells. PLoS ONE. 2011;6:e16394 pubmed publisher
  • chromatin immunoprecipitation; human
Brugat T, Nguyen Khac F, Grelier A, Merle Béral H, Delic J. Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells. Blood. 2010;116:239-49 pubmed publisher
  • western blot; human
Ohno M, Kunimoto M, Nishizuka M, Osada S, Imagawa M. Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression. Biochem Biophys Res Commun. 2009;390:738-42 pubmed publisher
  • western blot; human; 1:200
Li S, Kuhne W, Kulharya A, Hudson F, Ha K, Cao Z, et al. Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance. Nucleic Acids Res. 2009;37:6746-53 pubmed publisher
  • western blot; human
Bennett S, Neher T, Shatilla A, Turchi J. Molecular analysis of Ku redox regulation. BMC Mol Biol. 2009;10:86 pubmed publisher
  • immunocytochemistry; human
Murzik U, Hemmerich P, Weidtkamp Peters S, Ulbricht T, Bussen W, Hentschel J, et al. Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11. Mol Biol Cell. 2008;19:2926-35 pubmed publisher
  • western blot; human
Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R. Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo. Cancer Sci. 2008;99:810-5 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Luo M, Rosenke K, Czornak K, Fortunato E. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol. 2007;81:1934-50 pubmed
  • immunoprecipitation; human
  • western blot; human
Drouet J, Frit P, Delteil C, de Villartay J, Salles B, Calsou P. Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends. J Biol Chem. 2006;281:27784-93 pubmed
  • western blot; human
Biard D, Despras E, Sarasin A, Angulo J. Development of new EBV-based vectors for stable expression of small interfering RNA to mimick human syndromes: application to NER gene silencing. Mol Cancer Res. 2005;3:519-29 pubmed
  • western blot; human
Dutta A, Chakraborty A, Saha A, Ray S, Chatterjee A. Interaction of radiation- and bleomycin-induced lesions and influence of glutathione level on the interaction. Mutagenesis. 2005;20:329-35 pubmed
  • western blot; human
Ghoshal K, Datta J, Majumder S, Bai S, Kutay H, Motiwala T, et al. 5-Aza-deoxycytidine induces selective degradation of DNA methyltransferase 1 by a proteasomal pathway that requires the KEN box, bromo-adjacent homology domain, and nuclear localization signal. Mol Cell Biol. 2005;25:4727-41 pubmed
  • western blot; human
Drouet J, Delteil C, Lefrançois J, Concannon P, Salles B, Calsou P. DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks. J Biol Chem. 2005;280:7060-9 pubmed
Luo K, Huang W, Tang S. Sirt3 enhances glioma cell viability by stabilizing Ku70-BAX interaction. Onco Targets Ther. 2018;11:7559-7567 pubmed publisher
Nemoz C, Ropars V, Frit P, Gontier A, Drevet P, Yu J, et al. XLF and APLF bind Ku80 at two remote sites to ensure DNA repair by non-homologous end joining. Nat Struct Mol Biol. 2018;25:971-980 pubmed publisher
Róna G, Roberti D, Yin Y, Pagan J, Homer H, Sassani E, et al. PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. elife. 2018;7: pubmed publisher
Roth M, Wang Z, Chen W. SIRT1 and LSD1 competitively regulate KU70 functions in DNA repair and mutation acquisition in cancer cells. Oncotarget. 2016;7:50195-50214 pubmed publisher
Motiwala T, Zanesi N, Datta J, Roy S, Kutay H, Checovich A, et al. AP-1 elements and TCL1 protein regulate expression of the gene encoding protein tyrosine phosphatase PTPROt in leukemia. Blood. 2011;118:6132-40 pubmed publisher
Gorsler T, Murzik U, Ulbricht T, Hentschel J, Hemmerich P, Melle C. DNA damage-induced translocation of S100A11 into the nucleus regulates cell proliferation. BMC Cell Biol. 2010;11:100 pubmed publisher
Shi L, Qiu D, Zhao G, Corthesy B, Lees Miller S, Reeves W, et al. Dynamic binding of Ku80, Ku70 and NF90 to the IL-2 promoter in vivo in activated T-cells. Nucleic Acids Res. 2007;35:2302-10 pubmed
Audebert M, Salles B, Calsou P. Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining. J Biol Chem. 2004;279:55117-26 pubmed
Udayakumar D, Bladen C, Hudson F, Dynan W. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003;278:41631-5 pubmed
Calsou P, Delteil C, Frit P, Drouet J, Salles B. Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment. J Mol Biol. 2003;326:93-103 pubmed
Bertinato J, Tomlinson J, Schild Poulter C, Haché R. Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription. Gene. 2003;302:53-64 pubmed
Koike M, Kuroiwa A, Koike A, Shiomi T, Matsuda Y. Expression and chromosome location of hamster Ku70 and Ku80. Cytogenet Cell Genet. 2001;93:52-6 pubmed
Bischof O, Kim S, Irving J, Beresten S, Ellis N, Campisi J. Regulation and localization of the Bloom syndrome protein in response to DNA damage. J Cell Biol. 2001;153:367-80 pubmed
Jafri F, Hardin J, Dynan W. A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera. J Immunol Methods. 2001;251:53-61 pubmed
Lynch E, Moreland R, Ginis I, Perrine S, Faller D. Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro. Am J Physiol Cell Physiol. 2001;280:C897-911 pubmed
Bertinato J, Schild Poulter C, Hache R. Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits. J Cell Sci. 2001;114:89-99 pubmed
Frit P, Li R, Arzel D, Salles B, Calsou P. Ku entry into DNA inhibits inward DNA transactions in vitro. J Biol Chem. 2000;275:35684-91 pubmed
Koike M, Awaji T, Kataoka M, Tsujimoto G, Kartasova T, Koike A, et al. Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis. J Cell Sci. 1999;112 ( Pt 22):4031-9 pubmed
Galande S, Kohwi Shigematsu T. Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences. J Biol Chem. 1999;274:20521-8 pubmed
Reddy S, Alcantara O, Boldt D. Analysis of DNA binding proteins associated with hemin-induced transcriptional inhibition. The hemin response element binding protein is a heterogeneous complex that includes the Ku protein. Blood. 1998;91:1793-801 pubmed
product information
Product Type :
Antibody
Product Name :
Ku70 Monoclonal Antibody (N3H10)
Catalog # :
MA5-13110
Quantity :
500µL
Price :
US 345
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Hamster, Human, Mouse, Non-human primate, Xenopus laevis
Applications :
ChIP assay: 2 µg/10^6 cells, Flow Cytometry: 0.25 µg/test, Immunocytochemistry: 1:50-1:200, Immunofluorescence: 1:50 - 1:200, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 2 µg/mL, Western Blot: 0.25-0.5 µg/mL
Species :
Hamster, Human, Mouse, Non-human primate, Xenopus laevis
Clone :
N3H10
Isotype :
IgG2b
Storage :
4° C
Description :
The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Immunogen :
Human placental extract designated as PSE1-PL
Format :
Liquid
Applications w/Dilutions :
ChIP assay: 2 µg/10^6 cells, Flow Cytometry: 0.25 µg/test, Immunocytochemistry: 1:50-1:200, Immunofluorescence: 1:50 - 1:200, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 2 µg/mL, Western Blot: 0.25-0.5 µg/mL
Aliases :
5'-dRP lyase Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, DNA repair protein XRCC6, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa,X-ray repair cross-complementing protein 6, lupus Ku autoantigen protein p70, thyroid autoantigen 70kD (Ku antigen), thyroid
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA