EGFR Monoclonal Antibody (EGFR.1), Biotin | Invitrogen MA5-13045 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Monoclonal Antibody (EGFR.1), Biotin

catalog :

MA5-13045

quantity :

500 µL

price :

US 405.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

EGFR.1

reactivity :

human, domestic horse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human western blot; human	Oksvold M, Pedersen N, Forfang L, Smeland E. Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling. FEBS Lett. 2012;586:3575-81 pubmed publisher
immunoprecipitation; human	Freeman D, McDorman K, Ogbagabriel S, Kozlosky C, Yang B, Doshi S, et al. Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer. Mol Cancer. 2012;11:47 pubmed publisher
blocking or activating experiments; human	Minai L, Yeheskely Hayon D, Golan L, Bisker G, Dann E, Yelin D. Optical nanomanipulations of malignant cells: controlled cell damage and fusion. Small. 2012;8:1732-9 pubmed publisher
immunocytochemistry; human immunohistochemistry; human	Bertelsen V, Sak M, Breen K, Rødland M, Johannessen L, Traub L, et al. A chimeric pre-ubiquitinated EGF receptor is constitutively endocytosed in a clathrin-dependent, but kinase-independent manner. Traffic. 2011;12:507-20 pubmed publisher
immunocytochemistry; human; 1:100	Zeineldin R, Ning Y, Hudson L. The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2. J Histochem Cytochem. 2010;58:529-41 pubmed publisher
flow cytometry; human	Wegner C, Wegener C, Malerød L, Pedersen N, Progida C, Prodiga C, et al. Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5. Histochem Cell Biol. 2010;133:41-55 pubmed publisher
flow cytometry; human	Red Brewer M, Choi S, Alvarado D, Moravcevic K, Pozzi A, Lemmon M, et al. The juxtamembrane region of the EGF receptor functions as an activation domain. Mol Cell. 2009;34:641-51 pubmed publisher
flow cytometry; human	Pedersen N, Breen K, Rødland M, Haslekås C, Stang E, Madshus I. Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2. Mol Cancer Res. 2009;7:275-84 pubmed publisher
immunocytochemistry; human	Barr D, Ostermeyer Fay A, Matundan R, Brown D. Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells. J Cell Sci. 2008;121:3155-66 pubmed publisher
flow cytometry; human; 10 mg/ml	Offterdinger M, Bastiaens P. Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane. Traffic. 2008;9:147-55 pubmed
immunohistochemistry; human; 1:25	Goldstein N, Decker D, Severson D, Schell S, Vicini F, Margolis J, et al. Molecular classification system identifies invasive breast carcinoma patients who are most likely and those who are least likely to achieve a complete pathologic response after neoadjuvant chemotherapy. Cancer. 2007;110:1687-96 pubmed
immunocytochemistry; human	Sundelin K, Roberg K, Grenman R, Hakansson L. Effects of cisplatin, alpha-interferon, and 13-cis retinoic acid on the expression of Fas (CD95), intercellular adhesion molecule-1 (ICAM-1), and epidermal growth factor receptor (EGFR) in oral cancer cell lines. J Oral Pathol Med. 2007;36:177-83 pubmed
immunocytochemistry; human	Myromslien F, Grøvdal L, Raiborg C, Stenmark H, Madshus I, Stang E. Both clathrin-positive and -negative coats are involved in endosomal sorting of the EGF receptor. Exp Cell Res. 2006;312:3036-48 pubmed
immunocytochemistry; human	Johannessen L, Pedersen N, Pedersen K, Madshus I, Stang E. Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits. Mol Cell Biol. 2006;26:389-401 pubmed
immunocytochemistry; human	Haslekås C, Breen K, Pedersen K, Johannessen L, Stang E, Madshus I. The inhibitory effect of ErbB2 on epidermal growth factor-induced formation of clathrin-coated pits correlates with retention of epidermal growth factor receptor-ErbB2 oligomeric complexes at the plasma membrane. Mol Biol Cell. 2005;16:5832-42 pubmed
immunocytochemistry; human	Locke M, Heywood M, Fawell S, Mackenzie I. Retention of intrinsic stem cell hierarchies in carcinoma-derived cell lines. Cancer Res. 2005;65:8944-50 pubmed
immunocytochemistry; human	Longva K, Pedersen N, Haslekås C, Stang E, Madshus I. Herceptin-induced inhibition of ErbB2 signaling involves reduced phosphorylation of Akt but not endocytic down-regulation of ErbB2. Int J Cancer. 2005;116:359-67 pubmed
immunohistochemistry; human; 1:100	Odajima T, Sasaki Y, Tanaka N, Kato Mori Y, Asanuma H, Ikeda T, et al. Abnormal beta-catenin expression in oral cancer with no gene mutation: correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index, and clinicopathological features. Hum Pathol. 2005;36:234-41 pubmed
immunohistochemistry; human; 1:200	Lo H, Xia W, Wei Y, Ali Seyed M, Huang S, Hung M. Novel prognostic value of nuclear epidermal growth factor receptor in breast cancer. Cancer Res. 2005;65:338-48 pubmed
	Grøvdal L, Stang E, Sorkin A, Madshus I. Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation. Exp Cell Res. 2004;300:388-95 pubmed
	Witton C, Reeves J, Going J, Cooke T, Bartlett J. Expression of the HER1-4 family of receptor tyrosine kinases in breast cancer. J Pathol. 2003;200:290-7 pubmed
	Yu X, Sharma K, Takahashi T, Iwamoto R, Mekada E. Ligand-independent dimer formation of epidermal growth factor receptor (EGFR) is a step separable from ligand-induced EGFR signaling. Mol Biol Cell. 2002;13:2547-57 pubmed
	Ge H, Gong X, Tang C. Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis. Int J Cancer. 2002;98:357-61 pubmed
	O Bryan J, Lambert Q, Der C. The src homology 2 and phosphotyrosine binding domains of the ShcC adaptor protein function as inhibitors of mitogenic signaling by the epidermal growth factor receptor. J Biol Chem. 1998;273:20431-7 pubmed

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product information

Product Type :

Antibody

Product Name :

EGFR Monoclonal Antibody (EGFR.1), Biotin

Catalog # :

MA5-13045

Quantity :

500 µL

Price :

US 405.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Equine, Human

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: 2 µg/mL

Species :

Equine, Human

Clone :

EGFR.1

Isotype :

IgG2b, kappa

Storage :

4° C

Description :

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

A431 human epidermoid cells

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: 2 µg/mL

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2

more info or order :

Invitrogen product webpage