ErbB3 Monoclonal Antibody (2B5) | Invitrogen MA5-13040 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ErbB3 Monoclonal Antibody (2B5)

catalog :

MA5-13040

quantity :

500 µL

price :

US 496

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B5

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; loading ...; fig 6 immunocytochemistry; human; loading ...; fig 2b western blot; human; loading ...; fig 6	Szymanska M, Fosdahl A, Raiborg C, Dietrich M, LiestÃ¸l K, Stang E, et al. Interaction with epsin 1 regulates the constitutive clathrin-dependent internalization of ErbB3. Biochim Biophys Acta. 2016;1863:1179-88 pubmed publisher
immunoprecipitation; human; 1 ug/time western blot; human	Printsev I, Yen L, Sweeney C, Carraway K. Oligomerization of the Nrdp1 E3 ubiquitin ligase is necessary for efficient autoubiquitination but not ErbB3 ubiquitination. J Biol Chem. 2014;289:8570-8 pubmed publisher
immunohistochemistry; human	Lipton A, Goodman L, Leitzel K, Cook J, Sperinde J, Haddad M, et al. HER3, p95HER2, and HER2 protein expression levels define multiple subtypes of HER2-positive metastatic breast cancer. Breast Cancer Res Treat. 2013;141:43-53 pubmed
immunoprecipitation; mouse; 1:1000	Brouxhon S, Kyrkanides S, Teng X, O Banion M, Clarke R, Byers S, et al. Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers. Mol Carcinog. 2014;53:893-906 pubmed publisher
western blot; human; 1:100	Zeineldin R, Ning Y, Hudson L. The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2. J Histochem Cytochem. 2010;58:529-41 pubmed publisher
immunoprecipitation; human western blot; human	Najy A, Day K, Day M. The ectodomain shedding of E-cadherin by ADAM15 supports ErbB receptor activation. J Biol Chem. 2008;283:18393-401 pubmed publisher
western blot; human	Jiang X, Borgesi R, McKnight N, Kaur R, Carpenter C, Balk S. Activation of nonreceptor tyrosine kinase Bmx/Etk mediated by phosphoinositide 3-kinase, epidermal growth factor receptor, and ErbB3 in prostate cancer cells. J Biol Chem. 2007;282:32689-98 pubmed
western blot; rat	Liu W, Zscheppang K, Murray S, Nielsen H, Dammann C. The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells. Biochim Biophys Acta. 2007;1772:737-47 pubmed
immunocytochemistry; rat western blot; rat	Zscheppang K, Liu W, Volpe M, Nielsen H, Dammann C. ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells. Am J Physiol Lung Cell Mol Physiol. 2007;293:L429-35 pubmed
western blot; mouse	Zscheppang K, Korenbaum E, Bueter W, Ramadurai S, Nielsen H, Dammann C. ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells. Pediatr Pulmonol. 2006;41:1205-12 pubmed
	Jiang X, Chen S, Asara J, Balk S. Phosphoinositide 3-kinase pathway activation in phosphate and tensin homolog (PTEN)-deficient prostate cancer cells is independent of receptor tyrosine kinases and mediated by the p110beta and p110delta catalytic subunits. J Biol Chem. 2010;285:14980-9 pubmed publisher
	Matsunaga T, Davis J, Greene M. Adult rat otic placode-derived neurons and sensory epithelium express all four erbB receptors: a role in regulating vestibular ganglion neuron viability. DNA Cell Biol. 2001;20:307-19 pubmed

product information

Product Type :

Antibody

Product Name :

ErbB3 Monoclonal Antibody (2B5)

Catalog # :

MA5-13040

Quantity :

500 µL

Price :

US 496

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1 µg/mL

Species :

Human, Mouse, Rat

Clone :

2B5

Isotype :

IgG1

Storage :

4° C

Description :

ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Immunogen :

Recombinant rat c-erbB-3/HER-3 oncoprotein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 1 µg/mL

Aliases :

avian erythroblastosis oncogene B 3; avian erythroblastosis oncogene B 3 receptor; C76256; c-erbB3; c-erbB-3; C-errB-3; erb-b2 receptor tyrosine kinase 3; ERBB3; ErbB-3; Erbb3r; erbB3-S; Glial growth factor receptor; HER3; human epidermal growth factor receptor 3; LCCS2; MDA BF 1; MDA-BF-1; MGC88033; nuc-ErbB3; p180 ErbB3; p180-ErbB3; p45 sErbB3; p45-sErbB3; p85 sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)