Collagen II Monoclonal Antibody (6B3) | Invitrogen MA5-13026 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Collagen II Monoclonal Antibody (6B3)

catalog :

MA5-13026

quantity :

500 µL

price :

US 396.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6B3

reactivity :

tiger salamander, human, mouse, rat, chicken, bovine, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:100; fig 5c	Reppel L, Schiavi J, Charif N, Leger L, Yu H, Pinzano A, et al. Chondrogenic induction of mesenchymal stromal/stem cells from Wharton's jelly embedded in alginate hydrogel and without added growth factor: an alternative stem cell source for cartilage tissue engineering. Stem Cell Res Ther. 2015;6:260 pubmed publisher
immunohistochemistry; bovine; 1:100	Petrera M, De Croos J, Iu J, Hurtig M, Kandel R, Theodoropoulos J. Supplementation with platelet-rich plasma improves the in vitro formation of tissue-engineered cartilage with enhanced mechanical properties. Arthroscopy. 2013;29:1685-92 pubmed publisher
western blot; human; 1:500	van Deemter M, Kuijer R, Harm Pas H, Jacoba van der Worp R, Hooymans J, Los L. Trypsin-mediated enzymatic degradation of type II collagen in the human vitreous. Mol Vis. 2013;19:1591-9 pubmed
immunohistochemistry; human	Hartlev L, Nyengaard J, Thomsen J, Boel L, Stengaard Pedersen K, Hauge E. Application of design-based stereology for estimation of absolute volume and surface area of the articular and calcified cartilage compartments of undecalcified human femoral heads. J Microsc. 2013;251:133-43 pubmed publisher
immunohistochemistry; mouse	Craft A, Ahmed N, Rockel J, Baht G, Alman B, Kandel R, et al. Specification of chondrocytes and cartilage tissues from embryonic stem cells. Development. 2013;140:2597-610 pubmed publisher
immunocytochemistry; bovine; 1:50	Nosikova Y, Santerre J, Grynpas M, Kandel R. Annulus fibrosus cells can induce mineralization: an in vitro study. Spine J. 2013;13:443-53 pubmed publisher
immunohistochemistry; rat; 1:2000	Bach Gansmo F, Irvine S, Brüel A, Thomsen J, Birkedal H. Calcified cartilage islands in rat cortical bone. Calcif Tissue Int. 2013;92:330-8 pubmed publisher
immunocytochemistry; mouse	Komada Y, Yamane T, Kadota D, Isono K, Takakura N, Hayashi S, et al. Origins and properties of dental, thymic, and bone marrow mesenchymal cells and their stem cells. PLoS ONE. 2012;7:e46436 pubmed publisher
immunohistochemistry; bovine; 1:500	Nosikova Y, Santerre J, Grynpas M, Gibson G, Kandel R. Characterization of the annulus fibrosus-vertebral body interface: identification of new structural features. J Anat. 2012;221:577-89 pubmed publisher
immunohistochemistry; bovine; 1:100	Taylor D, Ahmed N, Hayes A, Ferguson P, Gross A, Caterson B, et al. Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes. J Histochem Cytochem. 2012;60:576-87 pubmed publisher
immunohistochemistry; mouse	Knutsson J, Bagger Sjoback D, von Unge M. Structural tympanic membrane changes in secretory otitis media and cholesteatoma. Otol Neurotol. 2011;32:596-601 pubmed publisher
immunohistochemistry; human; 1:100	Rui Y, Du L, Wang Y, Wang Y, Lui P, Tang T, et al. Bone morphogenetic protein 2 promotes transforming growth factor ?3-induced chondrogenesis of human osteoarthritic synovium-derived stem cells. Chin Med J (Engl). 2010;123:3040-8 pubmed
immunohistochemistry; human	Díaz Prado S, Muiños López E, Hermida Gómez T, Rendal Vázquez M, Fuentes Boquete I, de Toro F, et al. Multilineage differentiation potential of cells isolated from the human amniotic membrane. J Cell Biochem. 2010;111:846-57 pubmed publisher
immunocytochemistry; mouse	Rogerson F, Chung Y, Deutscher M, Last K, Fosang A. Cytokine-induced increases in ADAMTS-4 messenger RNA expression do not lead to increased aggrecanase activity in ADAMTS-5-deficient mice. Arthritis Rheum. 2010;62:3365-73 pubmed publisher
immunocytochemistry; human; 1 ug/ml	Nigro J, White J, Ramshaw J, Haylock D, Nilsson S, Werkmeister J. The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells. Biomaterials. 2010;31:5689-99 pubmed publisher
immunohistochemistry; human	Markway B, Tan G, Brooke G, Hudson J, Cooper White J, Doran M. Enhanced chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in low oxygen environment micropellet cultures. Cell Transplant. 2010;19:29-42 pubmed publisher
immunohistochemistry; human; 1:100	Hu J, Feng K, Liu X, Ma P. Chondrogenic and osteogenic differentiations of human bone marrow-derived mesenchymal stem cells on a nanofibrous scaffold with designed pore network. Biomaterials. 2009;30:5061-7 pubmed publisher
immunocytochemistry; human	Chan B, Hui T, Yeung C, Li J, Mo I, Chan G. Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine. Biomaterials. 2007;28:4652-66 pubmed
immunohistochemistry; bovine; 1:100 western blot; bovine	Gan L, Kandel R. In vitro cartilage tissue formation by Co-culture of primary and passaged chondrocytes. Tissue Eng. 2007;13:831-42 pubmed
immunohistochemistry; mouse	Hausser H, Ruegg M, Brenner R, Ksiazek I. Agrin is highly expressed by chondrocytes and is required for normal growth. Histochem Cell Biol. 2007;127:363-74 pubmed
immunohistochemistry; human; 1 ug/ml	Hashimoto J, Kariya Y, Miyazaki K. Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332). Stem Cells. 2006;24:2346-54 pubmed
immunohistochemistry; mouse	Yamazaki H, Tsuneto M, Yoshino M, Yamamura K, Hayashi S. Potential of dental mesenchymal cells in developing teeth. Stem Cells. 2007;25:78-87 pubmed
immunohistochemistry; tiger salamander	Hutchison C, Pilote M, Roy S. The axolotl limb: a model for bone development, regeneration and fracture healing. Bone. 2007;40:45-56 pubmed
immunohistochemistry; domestic rabbit; 1:4000	Ge Z, Goh J, Lee E. Selection of cell source for ligament tissue engineering. Cell Transplant. 2005;14:573-83 pubmed
	Li Q, Zhao F, Li Z, Duan X, Cheng J, Zhang J, et al. Autologous Fractionated Adipose Tissue as a Natural Biomaterial and Novel One-Step Stem Cell Therapy for Repairing Articular Cartilage Defects. Front Cell Dev Biol. 2020;8:694 pubmed publisher
	Kondo Y, Fu J, Wang H, Hoover C, McDaniel J, Steet R, et al. Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking. JCI Insight. 2018;3: pubmed publisher
	Esseltine J, Shao Q, Brooks C, SAMPSON J, Betts D, SÃ©guin C, et al. Connexin43 Mutant Patient-Derived Induced Pluripotent Stem Cells Exhibit Altered Differentiation Potential. J Bone Miner Res. 2017;32:1368-1385 pubmed publisher
	Séguin C, Grynpas M, Pilliar R, Waldman S, Kandel R. Tissue engineered nucleus pulposus tissue formed on a porous calcium polyphosphate substrate. Spine (Phila Pa 1976). 2004;29:1299-306; discussion 1306-7 pubmed
	Fussenegger M, Meinhart J, Höbling W, Kullich W, Funk S, Bernatzky G. Stabilized autologous fibrin-chondrocyte constructs for cartilage repair in vivo. Ann Plast Surg. 2003;51:493-8 pubmed
	He Q, Li Q, Yang L, Xu J. Immortalization of human articular chondrocytes and induction of their phenotype. Chin Med J (Engl). 2003;116:1351-6 pubmed
	Nakayama N, Duryea D, Manoukian R, Chow G, Han C. Macroscopic cartilage formation with embryonic stem-cell-derived mesodermal progenitor cells. J Cell Sci. 2003;116:2015-28 pubmed

image

image 1 :

Formalin-fixed, paraffin-embedded human fetus stained with Collagen II antibody using peroxidase-conjugate and AEC chromogen. Note staining of matrix in the cartilaginous plates.

product information

Product Type :

Antibody

Product Name :

Collagen II Monoclonal Antibody (6B3)

Catalog # :

MA5-13026

Quantity :

500 µL

Price :

US 396.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Amphibian, Chicken, Human, Mouse

Applications :

Immunohistochemistry (Paraffin): 1-2 µg/mL

Species :

Amphibian, Chicken, Human, Mouse

Clone :

6B3

Isotype :

IgG1

Storage :

4° C

Description :

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Immunogen :

Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1-2 µg/mL

Aliases :

alpha 1 type II procollagen; alpha 1 type IIA collagen; alpha 1 type IIA collagen precursor; alpha collagen type II; alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CB11; CG2A1A; CGA; Chondrocalcin; COL11A3; Col2; Col2a; col2a1; Col2a-1; col2a1.L; col2a1a; col2a1-a; col2a1-b; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen type II alpha 1 chain; collagen type IIA; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital); collagen, type II, alpha 1 L homeolog; COLLII; cyanogen bromide; Del1; disproportionate micromelia; Dmm; Lpk; M100856; MGC131516; pro alpha1 type II collagen; pro-alpha 1(II); pro-alpha-1 type II collagen; procollagen alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; Rgsc856; SEDC; STL1; type II collagen; type II collagen cyanogen bromide fragment CB10; type II collogen cyanogen bromide fragment CB8; unnamed protein product; X-col II; xcol2a1; XELAEV_18013523mg

more info or order :

Invitrogen product webpage