ErbB4 Monoclonal Antibody (H4.72.8 (Ab72)), Biotin | Invitrogen MA5-13013 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ErbB4 Monoclonal Antibody (H4.72.8 (Ab72)), Biotin

catalog :

MA5-13013

quantity :

500 µL

price :

US 405.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

H4.72.8 (Ab72)

reactivity :

black ferret, human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Jay J, Brunhoeber P, Smith M, Williams R, Sugarman M, Free H, et al. Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4). Histopathology. 2013;62:563-77 pubmed publisher
immunohistochemistry; rat; 1:1000	Janssen M, Leiva Salcedo E, Buonanno A. Neuregulin directly decreases voltage-gated sodium current in hippocampal ErbB4-expressing interneurons. J Neurosci. 2012;32:13889-95 pubmed publisher
blocking or activating experiments; human	Hua Y, Gorshkov K, Yang Y, Wang W, Zhang N, Hughes D. Slow down to stay alive: HER4 protects against cellular stress and confers chemoresistance in neuroblastoma. Cancer. 2012;118:5140-54 pubmed publisher
blocking or activating experiments; rat	Minor K, Bournat J, Toscano N, Giger R, Davies S. Decorin, erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 regulation of semaphorin 3A in central nervous system scar tissue. Brain. 2011;134:1140-55 pubmed publisher
blocking or activating experiments; black ferret; 20 ug/ml	Poluch S, Juliano S. Populations of radial glial cells respond differently to reelin and neuregulin1 in a ferret model of cortical dysplasia. PLoS ONE. 2010;5:e13709 pubmed publisher
blocking or activating experiments; rat; 30 ug/ml	Zhou Y, Besner G. Heparin-binding epidermal growth factor-like growth factor is a potent neurotrophic factor for PC12 cells. Neurosignals. 2010;18:141-51 pubmed publisher
immunocytochemistry; human	Proietti C, Rosemblit C, Beguelin W, Rivas M, Díaz Flaqué M, Charreau E, et al. Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth. Mol Cell Biol. 2009;29:1249-65 pubmed publisher
blocking or activating experiments; human; 10 ug/ml	Leach R, Kilburn B, Petkova A, Romero R, Armant D. Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor. Am J Obstet Gynecol. 2008;198:471.e1-7; discussion 471.e7-8 pubmed publisher
immunocytochemistry; rat	Longart M, Chatani Hinze M, Gonzalez C, Vullhorst D, Buonanno A. Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons. Brain Res Bull. 2007;73:210-9 pubmed
blocking or activating experiments; human	Wolff G, Chiang P, Smith S, Romero R, Armant D. Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells. Biol Reprod. 2007;77:53-60 pubmed
blocking or activating experiments; human; 5-30 ug/ml immunohistochemistry; human	Starr A, Greif J, Vexler A, Ashkenazy Voghera M, Gladesh V, Rubin C, et al. ErbB4 increases the proliferation potential of human lung cancer cells and its blockage can be used as a target for anti-cancer therapy. Int J Cancer. 2006;119:269-74 pubmed
blocking or activating experiments; human; 10 ug/ml	Armant D, Kilburn B, Petkova A, Edwin S, Duniec Dmuchowski Z, Edwards H, et al. Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor. Development. 2006;133:751-9 pubmed
western blot; human	Horak E, Heitner T, Robinson M, Simmons H, Garrison J, Russeva M, et al. Isolation of scFvs to in vitro produced extracellular domains of EGFR family members. Cancer Biother Radiopharm. 2005;20:603-13 pubmed
blocking or activating experiments; rat; 10 ug/ml immunocytochemistry; rat western blot; rat	Chen J, Tseng H, Dichter M, Zhang H, Greene M. Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons. DNA Cell Biol. 2005;24:553-62 pubmed
	O charoenrat P, Wongkajornsilp A, Rhys Evans P, Eccles S. Signaling pathways required for matrix metalloproteinase-9 induction by betacellulin in head-and-neck squamous carcinoma cells. Int J Cancer. 2004;111:174-83 pubmed
	Mahtouk K, Jourdan M, de Vos J, Hertogh C, Fiol G, Jourdan E, et al. An inhibitor of the EGF receptor family blocks myeloma cell growth factor activity of HB-EGF and potentiates dexamethasone or anti-IL-6 antibody-induced apoptosis. Blood. 2004;103:1829-37 pubmed
	Gilmour L, MacLeod K, McCaig A, Sewell J, Gullick W, Smyth J, et al. Neuregulin expression, function, and signaling in human ovarian cancer cells. Clin Cancer Res. 2002;8:3933-42 pubmed
	O charoenrat P, Modjtahedi H, Rhys Evans P, Court W, Box G, Eccles S. Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells. Cancer Res. 2000;60:1121-8 pubmed
	Elenius K, Paul S, Allison G, Sun J, Klagsbrun M. Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation. EMBO J. 1997;16:1268-78 pubmed

product information

Product Type :

Antibody

Product Name :

ErbB4 Monoclonal Antibody (H4.72.8 (Ab72)), Biotin

Catalog # :

MA5-13013

Quantity :

500 µL

Price :

US 405.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-dependent, Western Blot: 1:50-1:500

Species :

Human

Clone :

H4.72.8 (Ab72)

Isotype :

IgG2a

Storage :

4° C

Description :

ErbB 4 (Her-4, c-erbB-4, v-erbB-4) is a member of the class I receptor kinase family. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

Immunogen :

Extracellular domain of recombinant human c-erbB-4/HER-4 oncoprotein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Western Blot: 1:50-1:500

Aliases :

4ICD; ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; erb-b2 receptor tyrosine kinase 4; Erbb4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; Her4; human epidermal growth factor receptor 4; Mer4; OTTHUMP00000209432; p180erbB4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-4; s80HER4; Tyro-2; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v-erb-b2 avian erythroblastic leukemia viral oncogene 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4

more info or order :

Invitrogen product webpage