ErbB2 (HER-2) Monoclonal Antibody (N12) | Invitrogen MA5-12998 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ErbB2 (HER-2) Monoclonal Antibody (N12)

catalog :

MA5-12998

quantity :

500 µL

price :

US 454.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N12

reactivity :

hamsters, human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:100; fig s7 western blot; mouse; 1:100; fig s7 immunocytochemistry; human; 1:100; fig s8 western blot; human; 1:100; fig s8	Lee H, Noh H, Mun J, Gu C, Sever S, Park S. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Nat Commun. 2016;7:12799 pubmed publisher
immunocytochemistry; human	Bickford L, Sun J, Fu K, Lewinski N, Nammalvar V, Chang J, et al. Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy. Nanotechnology. 2008;19:315102 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Xia W, Liu Z, Zong R, Liu L, Zhao S, Bacus S, et al. Truncated ErbB2 expressed in tumor cell nuclei contributes to acquired therapeutic resistance to ErbB2 kinase inhibitors. Mol Cancer Ther. 2011;10:1367-74 pubmed publisher
immunoprecipitation; human	DeFazio Eli L, Strommen K, Dao Pick T, Parry G, Goodman L, Winslow J. Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action. Breast Cancer Res. 2011;13:R44 pubmed publisher
flow cytometry; human; 4 ug/ml	Larson J, Goodman L, Tan Y, DeFazio Eli L, Paquet A, Cook J, et al. Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens. Patholog Res Int. 2010;2010:814176 pubmed publisher
immunocytochemistry; human	Bickford L, Agollah G, Drezek R, Yu T. Silica-gold nanoshells as potential intraoperative molecular probes for HER2-overexpression in ex vivo breast tissue using near-infrared reflectance confocal microscopy. Breast Cancer Res Treat. 2010;120:547-55 pubmed publisher
immunoprecipitation; hamsters	Gilmer T, Cable L, Alligood K, Rusnak D, Spehar G, Gallagher K, et al. Impact of common epidermal growth factor receptor and HER2 variants on receptor activity and inhibition by lapatinib. Cancer Res. 2008;68:571-9 pubmed publisher
immunocytochemistry; human	Lowery A, Gobin A, Day E, Halas N, West J. Immunonanoshells for targeted photothermal ablation of tumor cells. Int J Nanomedicine. 2006;1:149-54 pubmed
immunocytochemistry; human	Hegde P, Rusnak D, Bertiaux M, Alligood K, Strum J, Gagnon R, et al. Delineation of molecular mechanisms of sensitivity to lapatinib in breast cancer cell lines using global gene expression profiles. Mol Cancer Ther. 2007;6:1629-40 pubmed
immunohistochemistry; human; 2 ug/ml	Oldford S, Robb J, Codner D, Gadag V, Watson P, Drover S. Tumor cell expression of HLA-DM associates with a Th1 profile and predicts improved survival in breast carcinoma patients. Int Immunol. 2006;18:1591-602 pubmed
western blot; human	Horak E, Heitner T, Robinson M, Simmons H, Garrison J, Russeva M, et al. Isolation of scFvs to in vitro produced extracellular domains of EGFR family members. Cancer Biother Radiopharm. 2005;20:603-13 pubmed
	Wang S, Lien H, Xia W, Chen I, Lo H, Wang Z, et al. Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2. Cancer Cell. 2004;6:251-61 pubmed
	Xia W, Liu L, Ho P, Spector N. Truncated ErbB2 receptor (p95ErbB2) is regulated by heregulin through heterodimer formation with ErbB3 yet remains sensitive to the dual EGFR/ErbB2 kinase inhibitor GW572016. Oncogene. 2004;23:646-53 pubmed
	Wu J. C-erbB2 oncoprotein and its soluble ectodomain: a new potential tumor marker for prognosis early detection and monitoring patients undergoing Herceptin treatment. Clin Chim Acta. 2002;322:11-9 pubmed

product information

Product Type :

Antibody

Product Name :

ErbB2 (HER-2) Monoclonal Antibody (N12)

Catalog # :

MA5-12998

Quantity :

500 µL

Price :

US 454.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:50-1:200, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent

Species :

Human

Clone :

N12

Isotype :

IgG1

Storage :

4° C

Description :

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Immunogen :

Intact SKBR-3 breast cancer cells

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:50-1:200, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent

Aliases :

avian erythroblastosis oncogene B 2; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); CD antigen CD340; CD340; c-erb B2/neu protein; c-erbB2; C-erbB-2; c-neu; epidermal growth factor receptor-related protein; erbb 2; ERBB2; erb-b2; Erbb-2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; erb-b2 receptor tyrosine kinase 2; her 2; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; Kiaa3023; metas; Metastatic lymph node gene 19 protein; mKIAA3023; MLN 19; MLN19; NEU; Neu oncogene; NEU proto-oncogene; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; p185neu; phospho ErbB2; proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

more info or order :

Invitrogen product webpage