CDK4 Monoclonal Antibody (DCS-35), Biotin | Invitrogen MA5-12981 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CDK4 Monoclonal Antibody (DCS-35), Biotin

catalog :

MA5-12981

quantity :

500 µL

price :

US 484

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

DCS-35

reactivity :

human, mouse, rat, pigs

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; mouse	Naoe H, Chiyoda T, Ishizawa J, Masuda K, Saya H, Kuninaka S. The APC/C activator Cdh1 regulates the G2/M transition during differentiation of placental trophoblast stem cells. Biochem Biophys Res Commun. 2013;430:757-62 pubmed publisher
western blot; human	Leake K, Singhal J, Nagaprashantha L, Awasthi S, Singhal S. RLIP76 regulates PI3K/Akt signaling and chemo-radiotherapy resistance in pancreatic cancer. PLoS ONE. 2012;7:e34582 pubmed publisher
western blot; human	Wang Y, Wang X, Yang Z, Zhu G, Chen D, Meng Z. Menthol inhibits the proliferation and motility of prostate cancer DU145 cells. Pathol Oncol Res. 2012;18:903-10 pubmed
immunoprecipitation; human immunoprecipitation; mouse	Mori M, Kitazume M, Ose R, Kurokawa J, Koga K, Osuga Y, et al. Death effector domain-containing protein (DEDD) is required for uterine decidualization during early pregnancy in mice. J Clin Invest. 2011;121:318-27 pubmed publisher
immunoprecipitation; human	Rocha A, Paternot S, Coulonval K, Dumont J, Soares P, Roger P. Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation. Mol Biol Cell. 2008;19:4814-25 pubmed publisher
immunoprecipitation; human western blot; human	LARREA M, Liang J, Da Silva T, Hong F, Shao S, Han K, et al. Phosphorylation of p27Kip1 regulates assembly and activation of cyclin D1-Cdk4. Mol Cell Biol. 2008;28:6462-72 pubmed publisher
immunoprecipitation; human western blot; human	Arakawa T, Yamamura T, Hattori T, Hayashi H, Mori A, Yoshida A, et al. Contribution of extracellular signal-regulated kinases to the IL-1-induced growth inhibition of human melanoma cells A375. Int Immunopharmacol. 2008;8:80-9 pubmed
western blot; rat	Xie T, Niu Y, Ge K, Lu S. Regulation of keratinocyte proliferation in rats with deep, partial-thickness scald: modulation of cyclin D1-cyclin-dependent kinase 4 and histone H1 kinase activity of M-phase promoting factor. J Surg Res. 2008;147:9-14 pubmed
western blot; human	Hudson T, Hartle D, Hursting S, Nunez N, Wang T, Young H, et al. Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms. Cancer Res. 2007;67:8396-405 pubmed
western blot; mouse; 1:200	Landis M, Brown N, Baker G, Shifrin A, Das M, Geng Y, et al. The LxCxE pRb interaction domain of cyclin D1 is dispensable for murine development. Cancer Res. 2007;67:7613-20 pubmed
immunoprecipitation; mouse	Pajalunga D, Mazzola A, Salzano A, Biferi M, De Luca G, Crescenzi M. Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007;176:807-18 pubmed
western blot; rat	Shan Y, Zhou W, Fu X, Yan H, Yang W, Liu S, et al. The role of p28GANK in rat oval cells activation and proliferation. Liver Int. 2006;26:240-7 pubmed
immunoprecipitation; mouse	Yu Q, Sicinska E, Geng Y, Ahnström M, Zagozdzon A, Kong Y, et al. Requirement for CDK4 kinase function in breast cancer. Cancer Cell. 2006;9:23-32 pubmed
immunoprecipitation; mouse western blot; mouse	Landis M, Pawlyk B, Li T, Sicinski P, Hinds P. Cyclin D1-dependent kinase activity in murine development and mammary tumorigenesis. Cancer Cell. 2006;9:13-22 pubmed
western blot; human; 1:200	Raynaud F, Whittaker S, Fischer P, McClue S, Walton M, Barrie S, et al. In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202. Clin Cancer Res. 2005;11:4875-87 pubmed
immunoprecipitation; human	Munoz Alonso M, Acosta J, Richard C, Delgado M, Sedivy J, Leon J. p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells. J Biol Chem. 2005;280:18120-9 pubmed
western blot; rat	Rodier G, Makris C, Coulombe P, Scime A, Nakayama K, Nakayama K, et al. p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2. J Cell Biol. 2005;168:55-66 pubmed
	James M, Ray A, Leznova D, Blain S. Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol. 2008;28:498-510 pubmed
	Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari K, Abraham T, et al. p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma. Int J Cancer. 2004;109:710-6 pubmed
	Whittaker S, Walton M, Garrett M, Workman P. The Cyclin-dependent kinase inhibitor CYC202 (R-roscovitine) inhibits retinoblastoma protein phosphorylation, causes loss of Cyclin D1, and activates the mitogen-activated protein kinase pathway. Cancer Res. 2004;64:262-72 pubmed
	Gingras B, Rodier G, Giasson E, Coulombe P, Chassagne C, Meloche S. Expression of angiotensin type II receptor downregulates Cdk4 synthesis and inhibits cell-cycle progression. Oncogene. 2003;22:2633-42 pubmed
	Ilyin G, Glaise D, Gilot D, Baffet G, Guguen Guillouzo C. Regulation and role of p21 and p27 cyclin-dependent kinase inhibitors during hepatocyte differentiation and growth. Am J Physiol Gastrointest Liver Physiol. 2003;285:G115-27 pubmed
	Lavictoire S, Parolin D, Klimowicz A, Kelly J, Lorimer I. Interaction of Hsp90 with the nascent form of the mutant epidermal growth factor receptor EGFRvIII. J Biol Chem. 2003;278:5292-9 pubmed
	Kogure T, Takasaki Y, Takeuchi K, Yamada H, Nawata M, Ikeda K, et al. Autoimmune responses to proliferating cell nuclear antigen multiprotein complexes involved in cell proliferation are strongly associated with their structure and biologic function in patients with systemic lupus erythematosus. Arthritis Rheum. 2002;46:2946-56 pubmed
	Lewis J, Thomas T, Klinge C, Gallo M, Thomas T. Regulation of cell cycle and cyclins by 16alpha-hydroxyestrone in MCF-7 breast cancer cells. J Mol Endocrinol. 2001;27:293-307 pubmed
	Latella L, Sacco A, Pajalunga D, Tiainen M, Macera D, D ANGELO M, et al. Reconstitution of cyclin D1-associated kinase activity drives terminally differentiated cells into the cell cycle. Mol Cell Biol. 2001;21:5631-43 pubmed
	Tsutsui T, Hesabi B, Moons D, Pandolfi P, Hansel K, Koff A, et al. Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity. Mol Cell Biol. 1999;19:7011-9 pubmed

product information

Product Type :

Antibody

Product Name :

CDK4 Monoclonal Antibody (DCS-35), Biotin

Catalog # :

MA5-12981

Quantity :

500 µL

Price :

US 484

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Porcine, Rat

Applications :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human, Mouse, Porcine, Rat

Clone :

DCS-35

Isotype :

IgG1, kappa

Storage :

4° C

Description :

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Immunogen :

Purified recombinant cdk4 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; p34. /cdk4; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)