c-Kit Monoclonal Antibody (K45) | Invitrogen MA5-12944 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

c-Kit Monoclonal Antibody (K45)

catalog :

MA5-12944

quantity :

500 µL

price :

US 395.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

K45

reactivity :

human, mouse, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 1	Kim J, Villadsen R. Expression of Luminal Progenitor Marker CD117 in the Human Breast Gland. J Histochem Cytochem. 2018;66:879-888 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 2e	Kondo J, Powell A, Wang Y, Musser M, Southard Smith E, Franklin J, et al. LRIG1 Regulates Ontogeny of Smooth Muscle-Derived Subsets of Interstitial Cells of Cajal in Mice. Gastroenterology. 2015;149:407-19.e8 pubmed publisher
western blot; mouse; 1:100	Wang Y, Shi C, Lu Y, Poulin E, Franklin J, Coffey R. Loss of Lrig1 leads to expansion of Brunner glands followed by duodenal adenomas with gastric metaplasia. Am J Pathol. 2015;185:1123-34 pubmed publisher
immunocytochemistry; human; 1:25 immunohistochemistry; human; 1:25	Balk Møller E, Kim J, Hopkinson B, Timmermans Wielenga V, Petersen O, Villadsen R. A marker of endocrine receptor-positive cells, CEACAM6, is shared by two major classes of breast cancer: luminal and HER2-enriched. Am J Pathol. 2014;184:1198-208 pubmed publisher
immunohistochemistry; pigs	Zhang W, Liu X, Yang L, Zhu D, Zhang Y, Chen Y, et al. Wharton's jelly-derived mesenchymal stem cells promote myocardial regeneration and cardiac repair after miniswine acute myocardial infarction. Coron Artery Dis. 2013;24:549-58 pubmed publisher
immunohistochemistry; human; 1:100	Fang S, Wei J, Pentinmikko N, Leinonen H, Salven P. Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell. PLoS Biol. 2012;10:e1001407 pubmed publisher
immunohistochemistry; human; 5 ug/ml	Lee H, Hennig G, Fleming N, Keef K, Spencer N, Ward S, et al. Septal interstitial cells of Cajal conduct pacemaker activity to excite muscle bundles in human jejunum. Gastroenterology. 2007;133:907-17 pubmed
immunohistochemistry; human; 5 ug/ml	Lee H, Hennig G, Fleming N, Keef K, Spencer N, Ward S, et al. The mechanism and spread of pacemaker activity through myenteric interstitial cells of Cajal in human small intestine. Gastroenterology. 2007;132:1852-65 pubmed
	Yan W, Zhu Z, Pan F, Huang A, Dai G. Overexpression of c-kit(CD117), relevant with microvessel density, is an independent survival prognostic factor for patients with HBV-related hepatocellular carcinoma. Onco Targets Ther. 2018;11:1285-1292 pubmed publisher
	Sinanan A, Hunt N, Lewis M. Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells. Biotechnol Appl Biochem. 2004;40:25-34 pubmed
	Cruz A, Frank B, Edwards S, Dazin P, Peschon J, Fang K. Tumor necrosis factor-alpha-converting enzyme controls surface expression of c-Kit and survival of embryonic stem cell-derived mast cells. J Biol Chem. 2004;279:5612-20 pubmed

image

image 1 :

Frozen human tonsil section stained with CD117 antibody using UltraVision LP and AEC chromogen. Note staining of mast cells.

product information

Product Type :

Antibody

Product Name :

c-Kit Monoclonal Antibody (K45)

Catalog # :

MA5-12944

Quantity :

500 µL

Price :

US 395.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent

Species :

Human

Clone :

K45

Isotype :

IgG2a

Storage :

4° C

Description :

c-Kit, also known as mast/stem cell growth factor receptor (SCFR) or CD117, is a trans membrane type III tyrosine kinase receptor encoded by the c-Kit proto-oncogene, located on chromosome 4 in humans. It is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Immunogen :

Recombinant human extracellular c-kit fragment

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent

Aliases :

belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W

more info or order :

Invitrogen product webpage