Ku70/Ku80 Monoclonal Antibody (162) | Invitrogen MA5-12938 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Ku70/Ku80 Monoclonal Antibody (162)

catalog :

MA5-12938

quantity :

500 uL

price :

US 414.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

162

reactivity :

human, mouse, Xenopus laevis

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 18

Reference
Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed publisher
Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res. 2011;39:9605-19 pubmed publisher
Choi Y, Nash K, Byrne B, Muzyczka N, Song S. The effect of DNA-dependent protein kinase on adeno-associated virus replication. PLoS ONE. 2010;5:e15073 pubmed publisher
De Zio D, Bordi M, Tino E, Lanzuolo C, Ferraro E, Mora E, et al. The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage. Cell Death Differ. 2011;18:516-27 pubmed publisher
Bombarde O, Boby C, Gomez D, Frit P, Giraud Panis M, Gilson E, et al. TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends. EMBO J. 2010;29:1573-84 pubmed publisher
Koike M, Koike A. Accumulation of Ku80 proteins at DNA double-strand breaks in living cells. Exp Cell Res. 2008;314:1061-70 pubmed publisher
Roberts S, Ramsden D. Loading of the nonhomologous end joining factor, Ku, on protein-occluded DNA ends. J Biol Chem. 2007;282:10605-13 pubmed
Drouet J, Frit P, Delteil C, de Villartay J, Salles B, Calsou P. Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends. J Biol Chem. 2006;281:27784-93 pubmed
Liu S, Cerutti A, Casali P, Crow M. Ongoing immunoglobulin class switch DNA recombination in lupus B cells: analysis of switch regulatory regions. Autoimmunity. 2004;37:431-443 pubmed publisher
Audebert M, Salles B, Calsou P. Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining. J Biol Chem. 2004;279:55117-26 pubmed
Sawchuk D, Mansilla Soto J, Alarcon C, Singha N, Langen H, Bianchi M, et al. Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule. J Biol Chem. 2004;279:29821-31 pubmed
Onclercq Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor Gueret M. Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing. Nucleic Acids Res. 2003;31:6272-82 pubmed
Wang H, Perrault A, Takeda Y, Qin W, Wang H, Iliakis G. Biochemical evidence for Ku-independent backup pathways of NHEJ. Nucleic Acids Res. 2003;31:5377-88 pubmed
Schild Poulter C, Matheos D, Novac O, Cui B, Giffin W, Ruiz M, et al. Differential DNA binding of Ku antigen determines its involvement in DNA replication. DNA Cell Biol. 2003;22:65-78 pubmed
Novac O, Matheos D, Araujo F, Price G, Zannis Hadjopoulos M. In vivo association of Ku with mammalian origins of DNA replication. Mol Biol Cell. 2001;12:3386-401 pubmed
Frit P, Li R, Arzel D, Salles B, Calsou P. Ku entry into DNA inhibits inward DNA transactions in vitro. J Biol Chem. 2000;275:35684-91 pubmed
Camara Clayette V, Thomas D, Rahuel C, Barbey R, Cartron J, Bertrand O. The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit. Nucleic Acids Res. 1999;27:1656-63 pubmed
Shao R, Cao C, Zhang H, Kohn K, Wold M, Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999;18:1397-406 pubmed

product information

Product Type :

Antibody

Product Name :

Ku70/Ku80 Monoclonal Antibody (162)

Catalog # :

MA5-12938

Quantity :

500 uL

Price :

US 414.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Xenopus laevis

Applications :

Flow Cytometry: 0.5 ug/test, Gel Shift: 1mg/mL, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Paraffin): 1-2 ug/mL

Species :

Human, Mouse, Non-human primate, Xenopus laevis

Clone :

162

Isotype :

IgG2a

Storage :

4 C

Description :

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008].

Immunogen :

Human B cell nuclei from plasmacytoid 2p68 cells

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 ug/test, Gel Shift: 1mg/mL, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Paraffin): 1-2 ug/mL

Aliases :

5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit of Ku antigen; 70kDa; 86 kDa subunit of Ku antigen; AI314015; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC75; CTC85; CTCBF; DNA repair protein XRCC5; DNA repair protein XRCC6; G22P1; G22P2; KARP1; KARP-1; Ku autoantigen p70 subunit; ku autoantigen protein p70 homolog; Ku autoantigen protein p86 homolog; Ku autoantigen, 70kDa; Ku autoantigen, 80kD); Ku autoantigen, 80kDa; Ku p70; Ku p80; KU70; Ku70 DNA-binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa); KU80; Ku86; Ku86 autoantigen related protein 1; KUB2; Kup70; Kup80; lupus Ku autoantigen protein p70; Lupus Ku autoantigen protein p86; ML8; NFIV; Nuclear factor IV; thyroid autoantigen; thyroid autoantigen 70 kDa; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); thyroid-lupus autoantigen; thyroid-lupus autoantigen p70; TLAA; XELAEV_18023498mg; XELAEV_18044412mg; X-ray repair complementing defective repair in Chinese hamster cells 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kDa); X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog; X-ray repair cross complementation (double-strand-break rejoining; X-ray repair cross complementation (double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross complementing 5; X-ray repair cross complementing 6; X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 6; xrcc5; xrcc5.L; xrcc5-a; Xrcc6; xrcc6.L