This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
NRG1 Monoclonal Antibody (7D5)
catalog :
MA5-12896
quantity :
500 µL
price :
US 450
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
7D5
reactivity :
human, mouse, rat, dogs, rhesus macaque
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 29
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; fig 2
Yang Z, Jiang Q, Chen S, Hu C, Shen H, Huang P, et al. Differential changes in Neuregulin-1 signaling in major brain regions in a lipopolysaccharide-induced neuroinflammation mouse model. Mol Med Rep. 2016;14:790-6 pubmed publisher
  • immunohistochemistry; rhesus macaque; 1:100
Zhao W. Expression and Localization of Neuregulin-1 (Nrg1) and ErbB2/ErbB4 Receptors in Main Endocrine Organs of the Rhesus Monkey. Int J Endocrinol Metab. 2013;11:162-6 pubmed publisher
  • immunohistochemistry; rat; 1:20
  • western blot; rat; 1:500
Zhao W, Ren S. Neuregulin-1 (Nrg1) is mainly expressed in rat pituitary gonadotroph cells and possibly regulates prolactin (PRL) secretion in a juxtacrine manner. J Neuroendocrinol. 2011;23:1252-62 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Hedhli N, Huang Q, Kalinowski A, Palmeri M, Hu X, Russell R, et al. Endothelium-derived neuregulin protects the heart against ischemic injury. Circulation. 2011;123:2254-62 pubmed publisher
  • immunocytochemistry; rat; 1:100
  • western blot; rat; 1:500
Zhao W, Shen Y, Ren S. Endogenous expression of Neuregulin-1 (Nrg1) as a potential modulator of prolactin (PRL) secretion in GH3 cells. Cell Tissue Res. 2011;344:313-20 pubmed publisher
  • western blot; human
Finigan J, Faress J, Wilkinson E, Mishra R, Nethery D, Wyler D, et al. Neuregulin-1-human epidermal receptor-2 signaling is a central regulator of pulmonary epithelial permeability and acute lung injury. J Biol Chem. 2011;286:10660-70 pubmed publisher
  • western blot; mouse; 1:1000
Kato T, Kasai A, Mizuno M, Fengyi L, Shintani N, Maeda S, et al. Phenotypic characterization of transgenic mice overexpressing neuregulin-1. PLoS ONE. 2010;5:e14185 pubmed publisher
  • immunoprecipitation; human
  • ELISA; human; 2 ug/ml
  • western blot; human; 1:1000
Moondra V, Sarma S, Buxton T, Safa R, Cote G, Storer T, et al. Serum Neuregulin-1beta as a Biomarker of Cardiovascular Fitness. Open Biomark J. 2009;2:1-5 pubmed
  • western blot; dogs
Doggen K, Ray L, Mathieu M, Mc Entee K, Lemmens K, De Keulenaer G. Ventricular ErbB2/ErbB4 activation and downstream signaling in pacing-induced heart failure. J Mol Cell Cardiol. 2009;46:33-8 pubmed publisher
  • western blot; human
Lok J, Sardi S, Guo S, Besancon E, Ha D, Rosell A, et al. Neuregulin-1 signaling in brain endothelial cells. J Cereb Blood Flow Metab. 2009;29:39-43 pubmed publisher
  • immunohistochemistry; mouse; 1:400
Kagitani Shimono K, Mohri I, Yagi T, Taniike M, Suzuki K. Peripheral neuropathy in the twitcher mouse: accumulation of extracellular matrix in the endoneurium and aberrant expression of ion channels. Acta Neuropathol. 2008;115:577-87 pubmed publisher
  • immunohistochemistry; human; 1:50
  • western blot; human
Sigala F, Georgopoulos S, Papalambros E, Chasiotis D, Vourliotakis G, Niforou A, et al. Heregulin, cysteine rich-61 and matrix metalloproteinase 9 expression in human carotid atherosclerotic plaques: relationship with clinical data. Eur J Vasc Endovasc Surg. 2006;32:238-45 pubmed
  • immunoprecipitation; rat
Lemmens K, Segers V, Demolder M, De Keulenaer G. Role of neuregulin-1/ErbB2 signaling in endothelium-cardiomyocyte cross-talk. J Biol Chem. 2006;281:19469-77 pubmed
  • immunohistochemistry; human
Bernstein H, Lendeckel U, Bertram I, Bukowska A, Kanakis D, Dobrowolny H, et al. Localization of neuregulin-1alpha (heregulin-alpha) and one of its receptors, ErbB-4 tyrosine kinase, in developing and adult human brain. Brain Res Bull. 2006;69:546-59 pubmed
  • immunoprecipitation; human; 2 ug/time
  • western blot; human; 1:200
Hansen M, Roehm P, Chatterjee P, Green S. Constitutive neuregulin-1/ErbB signaling contributes to human vestibular schwannoma proliferation. Glia. 2006;53:593-600 pubmed
  • immunohistochemistry; human; 1:50
Panutsopulos D, Arvanitis D, Tsatsanis C, Papalambros E, Sigala F, Spandidos D. Expression of heregulin in human coronary atherosclerotic lesions. J Vasc Res. 2005;42:463-74 pubmed
  • immunohistochemistry; human; 1:10
Prentice L, Shadeo A, Lestou V, Miller M, DeLeeuw R, Makretsov N, et al. NRG1 gene rearrangements in clinical breast cancer: identification of an adjacent novel amplicon associated with poor prognosis. Oncogene. 2005;24:7281-9 pubmed
  • immunohistochemistry; human; 1:100
Fountzilas G, Pectasides D, Kalogera Fountzila A, Skarlos D, Kalofonos H, Papadimitriou C, et al. Paclitaxel and carboplatin as first-line chemotherapy combined with gefitinib (IRESSA) in patients with advanced breast cancer: a phase I/II study conducted by the Hellenic Cooperative Oncology Group. Breast Cancer Res Treat. 2005;92:1-9 pubmed
  • western blot; human; 1:1000
Ritch P, Carroll S, Sontheimer H. Neuregulin-1 enhances survival of human astrocytic glioma cells. Glia. 2005;51:217-28 pubmed
Wang H, Cui W, Chen W, Liu F, Dong Z, Xing G, et al. The laterodorsal tegmentum-ventral tegmental area circuit controls depression-like behaviors by activating ErbB4 in DA neurons. Mol Psychiatry. 2023;28:1027-1045 pubmed publisher
Xu J, Guo C, Liu Y, Wu G, Ke D, Wang Q, et al. Nedd4l downregulation of NRG1 in the mPFC induces depression-like behaviour in CSDS mice. Transl Psychiatry. 2020;10:249 pubmed publisher
Lin W, Ou G, Lin J, Yi S, Yao W, Pan H, et al. Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma. Oncol Lett. 2020;20:326-336 pubmed publisher
Aldaregia J, Errarte P, Olazagoitia Garmendia A, Gimeno M, Uriz J, Gershon T, et al. Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma. Cancers (Basel). 2020;12: pubmed publisher
Elliott K, Millington G, Brugmann S. A novel role for cilia-dependent sonic hedgehog signaling during submandibular gland development. Dev Dyn. 2018;247:818-831 pubmed publisher
Lemmens K, Doggen K, De Keulenaer G. Activation of the neuregulin/ErbB system during physiological ventricular remodeling in pregnancy. Am J Physiol Heart Circ Physiol. 2011;300:H931-42 pubmed publisher
Kalinowski A, Plowes N, Huang Q, Berdejo Izquierdo C, Russell R, Russell K. Metalloproteinase-dependent cleavage of neuregulin and autocrine stimulation of vascular endothelial cells. FASEB J. 2010;24:2567-75 pubmed publisher
Miyazaki Y, Nakanishi Y, Hieda Y. Tissue interaction mediated by neuregulin-1 and ErbB receptors regulates epithelial morphogenesis of mouse embryonic submandibular gland. Dev Dyn. 2004;230:591-6 pubmed
Bao J, Wolpowitz D, Role L, Talmage D. Back signaling by the Nrg-1 intracellular domain. J Cell Biol. 2003;161:1133-41 pubmed
Hansen M, Vijapurkar U, Koland J, Green S. Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins. Hear Res. 2001;161:87-98 pubmed
product information
Product Type :
Antibody
Product Name :
NRG1 Monoclonal Antibody (7D5)
Catalog # :
MA5-12896
Quantity :
500 µL
Price :
US 450
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunohistochemistry (Paraffin): 1:25-1:50, Immunoprecipitation: 2 µg/mg protein lysate, Western Blot: 1-2 µg/mL
Species :
Human, Mouse, Rat
Clone :
7D5
Isotype :
IgG2a
Storage :
4° C
Description :
Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.
Immunogen :
Recombinant extracellular domain of rat NDF protein
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunohistochemistry (Paraffin): 1:25-1:50, Immunoprecipitation: 2 µg/mg protein lysate, Western Blot: 1-2 µg/mL
Aliases :
6030402G23Rik; Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; D230005F13Rik; GGF; GGF2; GGFII; Glial growth factor; glial growth factor 2; heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); HGL; HRG; HRG1; HRGA; HRGalpha; MST131; MSTP131; NDF; Neu differentiation factor; neuregulin 1; neuregulin 1 type III beta 3; Neuregulin-1; NRG1; NRG-1; NRG1-IT2; Pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; Sensory and motor neuron-derived factor; SMDF; type I neuregulin 1; type III neuregulin 1
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA