ErbB4 Monoclonal Antibody (H4.77.16 (Ab77)) | Invitrogen MA5-12888 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ErbB4 Monoclonal Antibody (H4.77.16 (Ab77))

catalog :

MA5-12888

quantity :

500 µL

price :

US 500.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H4.77.16 (Ab77)

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:300; loading ...; fig 2a	Muller T, Braud S, Jüttner R, Voigt B, Paulick K, Sheean M, et al. Neuregulin 3 promotes excitatory synapse formation on hippocampal interneurons. EMBO J. 2018;37: pubmed publisher
immunohistochemistry; mouse; 1:300; fig 6	Lasiene J, Komine O, Fujimori Tonou N, Powers B, Endo F, Watanabe S, et al. Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons. Acta Neuropathol Commun. 2016;4:15 pubmed publisher
flow cytometry; human	Parente Pereira A, Whilding L, Brewig N, van der Stegen S, Davies D, Wilkie S, et al. Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin. J Immunol. 2013;191:2437-45 pubmed publisher
immunohistochemistry; human	Jay J, Brunhoeber P, Smith M, Williams R, Sugarman M, Free H, et al. Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4). Histopathology. 2013;62:563-77 pubmed publisher
flow cytometry; human	Davies D, Foster J, van der Stegen S, Parente Pereira A, Chiapero Stanke L, Delinassios G, et al. Flexible targeting of ErbB dimers that drive tumorigenesis by using genetically engineered T cells. Mol Med. 2012;18:565-76 pubmed publisher
immunocytochemistry; rat	Longart M, Chatani Hinze M, Gonzalez C, Vullhorst D, Buonanno A. Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons. Brain Res Bull. 2007;73:210-9 pubmed
blocking or activating experiments; human; 5-30 ug/ml immunohistochemistry; human	Starr A, Greif J, Vexler A, Ashkenazy Voghera M, Gladesh V, Rubin C, et al. ErbB4 increases the proliferation potential of human lung cancer cells and its blockage can be used as a target for anti-cancer therapy. Int J Cancer. 2006;119:269-74 pubmed
ELISA; human	Horak E, Heitner T, Robinson M, Simmons H, Garrison J, Russeva M, et al. Isolation of scFvs to in vitro produced extracellular domains of EGFR family members. Cancer Biother Radiopharm. 2005;20:603-13 pubmed
immunohistochemistry; human; 1:100	Akayama Y, Takekida S, Ohara N, Tateiwa H, Chen W, Nakabayashi K, et al. Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum. Hum Reprod. 2005;20:2708-14 pubmed
	Mitchell R, Mikolajczak M, Kersten C, Fleetwood Walker S. ErbB1-dependent signalling and vesicular trafficking in primary afferent nociceptors associated with hypersensitivity in neuropathic pain. Neurobiol Dis. 2020;142:104961 pubmed publisher
	del Pino I, Brotons Mas J, Marques Smith A, Marighetto A, Frick A, Marin O, et al. Abnormal wiring of CCK+ basket cells disrupts spatial information coding. Nat Neurosci. 2017;20:784-792 pubmed publisher
	Dragowska W, Warburton C, Yapp D, Minchinton A, Hu Y, Waterhouse D, et al. HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization. Mol Cancer Res. 2004;2:606-19 pubmed
	Fleisig H, El Din El Husseini A, Vincent S. Regulation of ErbB4 phosphorylation and cleavage by a novel histidine acid phosphatase. Neuroscience. 2004;127:91-100 pubmed
	Shirakawa K, Tsuda H, Heike Y, Kato K, Asada R, Inomata M, et al. Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer. Cancer Res. 2001;61:445-51 pubmed
	Aigner A, Hsieh S, Malerczyk C, Czubayko F. Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol. Toxicology. 2000;144:221-8 pubmed
	Kunisue H, Kurebayashi J, Otsuki T, Tang C, Kurosumi M, Yamamoto S, et al. Anti-HER2 antibody enhances the growth inhibitory effect of anti-oestrogen on breast cancer cells expressing both oestrogen receptors and HER2. Br J Cancer. 2000;82:46-51 pubmed
	Tang C, Concepcion X, Milan M, Gong X, Montgomery E, Lippman M. Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo. Cancer Res. 1999;59:5315-22 pubmed

product information

Product Type :

Antibody

Product Name :

ErbB4 Monoclonal Antibody (H4.77.16 (Ab77))

Catalog # :

MA5-12888

Quantity :

500 µL

Price :

US 500.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 2 µg/mL, Immunoprecipitation: 2 µg/mg protein lysate

Species :

Human, Mouse, Rat

Clone :

H4.77.16 (Ab77)

Isotype :

IgG1

Storage :

4° C

Description :

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

Immunogen :

Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 2 µg/mL, Immunoprecipitation: 2 µg/mg protein lysate

Aliases :

4ICD; ALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; erb-b2 receptor tyrosine kinase 4; Erbb4; ERBB4 intracellular domain; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; Her4; human epidermal growth factor receptor 4; Mer4; OTTHUMP00000209432; p180erbB4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-4; s80HER4; Tyro-2; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v-erb-b2 avian erythroblastic leukemia viral oncogene 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4